期刊论文

Chen, Jiwang

英文

Peptides

0196-9781

2012

33

1

52-58

10.1016/j.peptides.2011.11.006

National Advanced Science and Technology (863) Key ProgramNational High Technology Research and Development Program of China [2010AA023003]; 111 ProjectMinistry of Education, China - 111 Project [B07029]; earmarked fund for Modern Agro-industry Technology Research System [NYCYTX-49]; Hubei Provincial Ministry of Education [C2010040]; [PCSIRT0627]

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Peptides inhibiting angiotensin-I converting enzyme (ACE, EC. 3.4.15.1) are possible cures of hypertension. Food-derived ACE-inhibitory peptides are particularly attractive because of reduced side effects. Previously, we reported ACE-inhibitory activity of grass carp protein hydrolysates. In this work, we report steps for purifying the ACE-inhibitory peptide from the hydrolysate and its biochemical properties. Following steps of ultrafiltration, macroporous adsorption resin, and two steps of reversed phase high performance liquid chromatography (RE-HPLC), a single Val-Ala-Pro (VAP) tripeptide ...