作者机构：
[Yi, Liu; Xu, Zhao] School of Electrical and Electronic Engineering, Wuhan Polytechnic University, Wuhan, 430023, Hubei, China;[Lisha, Xu] School of Physics, Hubei University, Wuhan, 430062, China;[Yunjun, Mei] School of Chemical and Environmental Engineering, Wuhan Polytechnic University, Wuhan, 430023, Hubei, China;[Luocheng, Chen] Hubei Sino-Australian Nano Material Technology Co., Ltd., Guangshui, 432700, China;[Kuibo, Yin; Anqi, Zheng] SEU-FEI Nano-Pico Center, Key Laboratory of MEMS of Ministry of Education, Southeast University, Nanjing, 210096, People's Republic of China
摘要：
In the last few decades, nanoparticles have been a prominent topic in various fields, particularly in agriculture, due to their unique physicochemical properties. Herein, molybdenum copper lindgrenite Cu(3)(MoO(4))(2)(OH)(2) (CM) nanoflakes (NFs) are synthesized by a one-step reaction involving α-MoO(3) and CuCO(3)⋅Cu(OH)(2)⋅xH(2)O solution at low temperature for large scale industrial production and developed as an effective antifungal agent for the oilseed rape. This synthetic method demonstrates great potential for industrial applications. Infrared spectroscopy and X-ray diffraction (XRD) results reveal that CM samples exhibit a pure monoclinic structure. TG and DSC results show the thermal stable properties. It can undergo a phase transition form copper molybdate (Cu(3)Mo(2)O(9)) at about 300°C. Then Cu(3)Mo(2)O(9) nanoparticlesdecompose into at CuO and MoO(3) at 791°C. The morphology of CM powder is mainly composed of uniformly distributed parallelogram-shaped nanoflakes with an average thickness of about 30nm. Moreover, the binding energy of CM NFs is measured to be 2.8eV. To assess the antifungal properties of these materials, both laboratory and outdoor experiments are conducted. In the pour plate test, the minimum inhibitory concentration (MIC) of CM NFs against Sclerotinia sclerotiorum (S. sclerotiorum) is determined to be 100ppm, and the zone of inhibiting S. sclerotiorum is 14mm. When the concentration is above 100nm, the change rate of the hyphae circle slows down a little and begins to decrease until to 200ppm. According to the aforementioned findings, the antifungal effects of a nano CM NFs solution are assessed at different concentrations (0ppm (clear water), 40ppm, and 80ppm) on the growth of oilseed rape in an outdoor setting. The results indicate that the application of CM NFs led to significant inhibition of S. sclerotiorum. Specifically, when the nano CM solution was sprayed once at the initial flowering stage at a concentration of 80ppm, S. sclerotiorum growth was inhibited by approximately 34%. Similarly, when the solution was sprayed once at the initial flowering stage and once at the rape pod stage, using a concentration of 40ppm, a similar level of inhibition was achieved. These outcomes show that CM NFs possess the ability to bind with more metal ions due to their larger specific surface area. Additionally, their semiconductor physical properties enable the generation of reactive oxygen species (ROS). Therefore, CM NFs hold great potential for widespread application in antifungal products.
作者机构：
[Wang, Ruonan; Cao, Qiang; Wang, Xiaoliang; Li, Fengchang] The Institute of Technological Sciences, Wuhan University, Wuhan 430072, China;[Tian, Xiaoyu] School of Mechanical Engineering, Wuhan Polytechnic University, Wuhan 430048, China
通讯机构：
[Qiang Cao] T;The Institute of Technological Sciences, Wuhan University, Wuhan 430072, China
关键词：
Beam shaping;Femtosecond lasers;Lithium niobate;Nonlinear frequency conversion;Nonlinear photonic crystals;Ultrafast lasers
摘要：
We experimentally extend the nonlinear Gaussian to flat-top beam shaping from one to two dimensions through a three-dimensional nonlinear photonic crystal. Employing a near-infrared femtosecond laser, we induce a modification inside lithium niobate to achieve a second-order nonlinear optical coefficient modulation in three dimensions. The flat-topped truncation of wavefront has been adjusted in a mutual perpendicular coordinate separately. Among the generated flat-topped beams, the optimal flatness is 97.1%, and the nonlinear conversion efficiency is 10(-2) at the peak power of 37 kW with the interaction length of 630 µm. By adding an extra dimension, our work simultaneously enables full-wavefront flat-top distribution and nonlinear frequency conversion.
作者机构：
[Liu, Gang; Zhou, Wenhao; Zhang, Haizhi; Zhou, Lian; Li, Yaochang; Qin, Xinguang] College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China;[Liu, Gang; Zhang, Haizhi] Key Laboratory for Deep Processing of Major Grain and Oil (Wuhan Polytechnic University), Ministry of Education 430023, China;[Qin, Xinguang] Key Laboratory for Deep Processing of Major Grain and Oil (Wuhan Polytechnic University), Ministry of Education 430023, China. Electronic address: qxg198304@163.com
通讯机构：
[Qin, Xinguang] K;Key Laboratory for Deep Processing of Major Grain and Oil (Wuhan Polytechnic University), Ministry of Education 430023, China. Electronic address:
关键词：
Accelerated oxidation;Emulsion stability;Glycosylation;Lipid storage;Whey protein isolate
摘要：
Glycosylated protein was obtained by the reaction of whey protein isolate(WPI) with inulin of different polymerization degrees and was used to stabilize a pomegranate seed oil emulsion. The physicochemical and antioxidative properties of the emulsions were assessed, and the impacts of accelerated oxidation on pomegranate seed oil were examined. The interfacial tension of WPI and short-chain inulin (SCI)-glycosylated conjugate (WPI-SCI) gradually decreased with increasing glycosylation reaction time. Emulsions stabilized by WPI-SCI (72h) were the most stable, with a thick interfacial film on the surface of the droplets. After accelerated oxidation for 72h, WPI-SCI inhibited the oxidation of oil in the emulsion. GC-IMS results showed that the production of harmful volatile components in oil was inhibited, and the peroxide strength was less than 30mmol/kg oil. This study contributes to understanding of stable storage of lipids.
摘要：
Hybrid composites are widely regarded as the most promising choice for anode material in sodium -ion batteries. However, integrating high capacity and excellent cyclic stability into composites remains challenging. This work demonstrates a thin -liquid -layer polymerization approach to fabricate an integrated porous polypyrrole/carbon composite (PPy/C) for a high-performance sodium storage anode. By adjusting the monomer concentration reasonably and combining with heterostructure engineering, the optimized PPy/C was used as the anode for sodium -ion batteries, achieving rapid electrochemical kinetics and satisfactory reversible capacity. The reaction mechanism between PPy and Na+ in the Faraday reaction was revealed by ex -situ electrochemical impedance spectroscopy (EIS). DFT calculations illustrate that the synergistic effect between porous carbon and PPy can regulate the electron transfer mode around Na atoms, which contributes to anchoring Na in the PPy/C composite structure. Moreover, the PPy/C offers an ultrahigh capacity of 552 mA h g-1 at 0.1 A g-1 and a capacity retention rate of 86.5 % after 200 cycles. A full cell assembled with PPy/C and Na3V2(PO4)3 demonstrates a high energy density of 243.1 W h kg-1 at 125 W kg- 1 power density. This work would offer valuable thought for advancing the practical application of conductive polymer/carbon composites.
摘要：
The photocatalytic valorization of glucose has attracted more and more attention for producing value-added chemicals. Herein, value-added chemicals like arabinose and formic acid were obtained by selective photocatalytic oxidation of glucose irradiated with visible light, which was realized using Bi2O3/TiO2 composite as the photocatalyst and atmospheric O2 as the oxidant in water. It was found that the photocatalytic performance of Bi2O3/TiO2 composite in the glucose oxidation was obviously superior to that of pure Bi2O3 and pure TiO2, the significantly enhanced photocatalytic efficiency was mainly attributed to the promotion of photogenerated charge separation and adsorption capacity towards glucose. The influences of mass ratio of Bi2O3 to TiO2 and initial concentration of glucose on the glucose oxidation were further investigated with many details. Additionally, recycling experiments showed that the Bi2O3/TiO2 composite exhibited a favorable stability in the photocatalytic process, and the roles of various active species for glucose oxidation were also determined by the active species trapping experiments. This work could provide some valuable viewpoints for producing value-added chemicals from biomass by photocatalysis.
摘要：
Weeds are a significant threat to agricultural productivity and the environment. The increasing demand for sustainable weed control practices has driven innovative developments in alternative weed control technologies aimed at reducing the reliance on herbicides. The barrier to adoption of these technologies for selective in-crop use is availability of suitably effective weed recognition. With the great success of deep learning in various vision tasks, many promising image-based weed detection algorithms have been developed. This paper reviews recent developments of deep learning techniques in the field of image-based weed detection. The review begins with an introduction to the fundamentals of deep learning related to weed detection. Next, recent advancements in deep weed detection are reviewed with the discussion of the research materials including public weed datasets. Finally, the challenges of developing practically deployable weed detection methods are summarized, together with the discussions of the opportunities for future research. We hope that this review will provide a timely survey of the field and attract more researchers to address this inter-disciplinary research problem.
作者机构：
[Xia, Yang; Ho, Wingkei] Educ Univ Hong Kong, Dept Sci & Environm Studies, Tai Po, Hong Kong 999077, Peoples R China.;[Xia, Yang; Ho, Wingkei] Educ Univ Hong Kong, Ctr Environm & Sustainable Dev CESD, Tai Po, Hong Kong 999077, Peoples R China.;[Zhu, Bicheng; Yu, Jiaguo; Yu, JG] China Univ Geosci, Fac Mat Sci & Chem, Lab Solar Fuel, Wuhan 430074, Peoples R China.;[Xia, Yang] Wuhan Inst Technol, Sch Chem Engn & Pharm, Minist Educ, Key Lab Green Chem Proc, Wuhan 430205, Peoples R China.;[Ho, Wingkei] City Univ Hong Kong, State Key Lab Marine Pollut, Kowloon, Tat Chee Ave, Hong Kong, Peoples R China.
通讯机构：
[Ho, WK ] E;[Yu, JG ] C;Educ Univ Hong Kong, Dept Sci & Environm Studies, Tai Po, Hong Kong 999077, Peoples R China.;Educ Univ Hong Kong, Ctr Environm & Sustainable Dev CESD, Tai Po, Hong Kong 999077, Peoples R China.;China Univ Geosci, Fac Mat Sci & Chem, Lab Solar Fuel, Wuhan 430074, Peoples R China.
摘要：
Sluggish charge kinetics and moderate adsorption-desorption ability of gas molecules are major limitations for photocatalytic NOx elimination of bulk g-C3N4. A hierarchical porous g-C3N4 photocatalyst modified with N vacancies and charge channels (KCNN) was prepared by thermal polymerisation in KCl medium followed by quenching to increase the photocatalytic efficiency. The optimized KCNN sample exhibits highly enhanced photocatalytic NO removal rate (70.5%), which is superior to those of bulk g-C3N4 (38.1%), porous g-C3N4 (54.5%) and K-doped g-C3N4 (58.6%), respectively. X-ray photoelectron spectroscopy and electron paramagnetic resonance data reveal the successful formation of N vacancy in g-C3N4 framework. The enhanced activity of KCNN is ascribed to the enlarged surface area, expanded light absorption, low charge recombination efficiency and strong oxidation capability, respectively. In situ DRIFTS and density functional theory results suggest that the introduction of N vacancies and K+ ions enable control over NO adsorption and activation, leading to the implementation of a preferred pathway (NO -> NO+ -> NO3-) and reduction in the emission of toxic in-termediates. This work presents a potential idea for improving the charge transfer of layered materials and optimising the diffusion/adsorption/activation of gas molecules for photocatalytic NO oxidation.
作者机构：
[Cai, Jie; Xie, F; Xie, Fang; Zhang, Die] Wuhan Polytech Univ, Natl R&D Ctr Se Rich Agr Prod Proc, Hubei Engn Res Ctr Deep Proc Green Se Rich Agr Pro, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.;[Cai, Jie; Zhang, Die] Wuhan Polytech Univ, Key Lab Deep Proc Major Grain & Oil, Hubei Key Lab Proc & Transformat Agr Prod, Minist Educ, Wuhan 430023, Peoples R China.
通讯机构：
[Cai, J; Xie, F ] W;Wuhan Polytech Univ, Natl R&D Ctr Se Rich Agr Prod Proc, Hubei Engn Res Ctr Deep Proc Green Se Rich Agr Pro, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.
作者机构：
[Shu, Jing; Hu, Jie; Wang, Zhen; Liang, Hongtan; Xu, Bing; Zhang, Zhipeng; Zhang, Lei; Fan, Guozhi; Pan, Dehua] Hubei Provincial Engineering Technology Research Center of Agricultural and Sideline Resources, Chemical Engineering and Utilization, School of Chemistry and Environmental Engineering, Wuhan Polytechnic University Wuhan 430023 China xubing200806@163.com
通讯机构：
[Xu, Bing] H;Hubei Provincial Engineering Technology Research Center of Agricultural and Sideline Resources, Chemical Engineering and Utilization, School of Chemistry and Environmental Engineering, Wuhan Polytechnic University Wuhan 430023 China
摘要：
Polyimide aerogels (PIAs) not only possess excellent thermodynamic properties but also have a high porosity structure, making them an exceptional protective and thermal insulation material, and further broadening their application scope in aerospace and other cutting-edge fields. In this work, a series of anisotropic polyimide aerogels (3,3',4,4'-biphenyltetracarboxylic dianhydride (S-BPDA), p-phenylenediamine (PDA), 4,4'-diaminodiphenyl ether (ODA)) with excellent properties were prepared. These PIAs were obtained by unidirectional freeze-drying and thermal amination of two different precursor solutions mixed in proportion. These PIAs possess an irregularly oval tubular structure, exhibiting pronounced anisotropy. (PIA-2 exhibits outstanding flexible resilience in the radial direction. It can still regain its original form after half an hour of compression by a universal testing machine, yet it cannot do so in the axial direction. The thermal diffusivity of PIA-5 in the radial direction at room temperature is as low as 0.067 mm(2) s(-1), and even at 200 °C, the thermal diffusivity is as low as 0.057 mm(2) s(-1). Meanwhile, the thermal diffusivity in the axial direction at room temperature is 0.11 mm(2) s(-1), surpassing the value of 0.106 mm(2) s(-1) of aerogels prepared from monomeric raw materials and dried under supercritical conditions). PIAs exhibit outstanding thermal stability (the axial strength and modulus retention of PIA-8 at 200 °C are as high as 52.63% and 44.82%), and its weight loss temperature of 5% is as high as 603 °C and it has a glass softening temperature of 387 °C. PIAs also demonstrate exceptional flame retardancy in imitation flame retardant experiments and exhibit outstanding thermal insulation performance when heated on a 150 °C heating plate for 10 minutes (the radial surface temperature of PIA-5 was only 49.9 °C). These anisotropic PIAs materials exhibit outstanding flexible resilience, and thermal protection performance, holding significant importance for their widespread adoption as thermal insulation materials in aerospace, high-precision electronic components, and other domains.
摘要：
To investigate crack propagation and the coalescence mechanism of a rock bridge under unloading condition induced by intensive excavation of rock mass, the direct shear test with unloading normal stress and corresponding particle flow code (PFC) simulation were conducted on the sandstone specimen containing a parallel fissure pair considering different fissure inclinations (varied from 0 degrees to 90 degrees) and initial shear stresses (varied from 4 to 7 MPa). Three failure patterns (i.e., shear failure, tensile failure, and tensile-shear mixed failure) are identified as experimental and numerical results. The failure pattern transforms in the order of a shear, tensile, and tensile-shear mixed failure pattern as the fissure inclination increases. Three displacement field types are summarized and correspond to different failure patterns. Comparing the shear strength, cracking process, and microscopic displacement field in the direct shear test with unloading normal stress and the conventional direct shear test, normal unloading weakens the shear strength of the specimen under the selected stress conditions (initial normal stress is 20 MPa, initial shear stress ranges from 4 to 7 MPa). Rebound deformation in the process of unloading promotes the high proportion of tensile cracks for the tested fissure inclinations.
作者：
Ji, Yan;Cheng, Rui;Zhou, Xuan;Zhang, Jiaqi;Liu, Xiaodan;...
期刊：
Fish & Shellfish Immunology,2024年148:109466 ISSN：1050-4648
通讯作者：
Zhang, Chi
作者机构：
[Ji, Yan; Zhang, Jiaqi] Hubei Key Laboratory of Animal Nutrition and Feed Science, School of Animal Science and Nutritional Engineering, Wuhan Polytechnic University, Wuhan, 430023, China;[Cheng, Rui] Key Laboratory of Ecological Impacts of Hydraulic-Projects and Restoration of Aquatic Ecosystem of Ministry of Water Resources, Institute of Hydroecology, MWR &CAS, Wuhan, 430070, China;[Zhou, Xuan] Technology Center of Wuhan Customs, Wuhan, 430050, China;[Liu, Xiaodan] College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China;[Sheng, Suhong] Huzhou Shengjiang Fishery Co., LTD, Huzhou, 313018, China
通讯机构：
[Zhang, Chi] H;Hubei Key Laboratory of Animal Nutrition and Feed Science, School of Animal Science and Nutritional Engineering, Wuhan Polytechnic University, Wuhan, 430023, China. Electronic address:
摘要：
To evade host antiviral response, viruses have evolved to take advantage of their noncoding RNAs (ncRNAs). Snakehead vesiculovirus (SHVV), a newly isolated fish rhabdovirus from diseased hybrid snakehead, has caused high mortality to the cultured snakehead fish during the past years in China. However, little is known about the mechanisms of its pathogenicity. Our study revealed that overexpression of the 30-nt leader RNA promoted SHVV replication. RNA-protein binding investigation revealed that SHVV leader RNA could interact with host 40S ribosomal protein S8 (RPS8) and 60S ribosomal protein L13a (L13a). Furthermore, we found that SHVV infection upregulated RPS8 and L13a, and in turn, overexpression of RPS8 or L13a inhibited, while knockdown of RPS8 or L13a promoted, SHVV replication, suggesting that RPS8 and L13a acted as host antiviral factors in response to SHVV infection. In addition, our study revealed that RPS8- or L13a-mediated inhibition of SHVV replication could be restored by co-transfection with leader RNA, suggesting that the interaction between leader RNA and RPS8 or L13a might affect the anti-SHVV effects of RPS8 and L13a. Taken together, these results suggest that SHVV leader RNA can interact with the host antiviral factors RPS8 and L13a, and promote SHVV replication. This study provides a better understanding of the molecular mechanism of the pathogenesis of SHVV and a potential antiviral strategy against SHVV infection.
摘要：
The contribution systematically reviews the conceptual proposition, mechanism, catalyst species, applications, existing controversies and future developmental trends of self-immobilized polyolefin catalysts (SIPCs). To avoid the adverse effects in heterogenization of single site catalysts, the concept of self-immobilization was designed in order to not only keep the single site characteristics of catalysts but also improve polyolefin morphology control to reliver reactor fouling. The self-immobilized polyolefin catalysts have extended their territory from early-transition-metal to late-transition-metal ones, and been applied to produce high value-added polyolefins. Due to the lack of direct evidences, the classic self-immobilization mechanism invokes huge controversies, and the nature of self-immobilization needs to be explored in depth and explained by a more insightful theory. Fortunately, the self-immobilized polyolefin catalysts have been developed into the flexible immobilization modes of more robust product morphology control.
摘要：
Microplastics released from plastic-based filter bags during tea brewing have attracted widespread attention. Laser confocal micro-Raman and direct classical least squares were used to identify and estimate micron-sized microplastics. Characteristic peaks from pyrolysis-gas chromatography/mass spectrometry of polyethylene terephthalate, polypropylene, and nylon 6 were selected to construct curves for quantification submicron-sized microplastics. The results showed that microplastics released from tea bags in the tea infusions ranged from 80 to 1288 pieces (micron-sized) and 0 to 63.755μg (submicron-sized) per filter bag. Nylon 6 woven tea bags released far fewer microplastics than nonwoven filter bags. In particular, a simple strategy of three pre-washes with room temperature water significantly reduced microplastic residues with removal rates of 76%-94% (micron-sized) and 80%-87% (submicron-sized), respectively. The developed assay can be used for the quantitative evaluation of microplastics in tea infusions, and the pre-washing reduced the risk of human exposure to microplastics during tea consumption.
摘要：
The work aimed to explore the effect of supercritical carbon dioxide (SCCD) treatment on the enzyme activity, color, carotenoid, sugar and phenolic profile, and antioxidant activity of fresh-cut pumpkins. The polyphenol oxidase (PPO) and peroxidase (POD) activities of SCCD-treated pumpkin cubes were reduced by 21% and 18%, respectively. The lightness value of SCCD-treated samples declined compared to the untreated samples, whereas the redness and yellowness values in treated samples were enhanced. The lutein, alpha-carotene, beta-carotene and total carotenoids, glucose, sorbitol, and other polysaccharides in the SCCD-treated samples showed a fluctuation trend. Similarly, the treated sample's total phenolic contents (TPC), ABTS (2,2 '- azino-bis(3ethylbenzothiazoline-6-sulfonic acid, DPPH (2,2-diphenyl-1-picrylhydrazyl), and superoxide radical scavenging activities were increased and then reduced. An enhancement was noted in the coumaryloquinic acid I and II, caffeic acid glucoside, 4-hydroxybenzoic acid, and p-coumaric acid of SCCD-treated samples. The work demonstrated that the SCCD technique at moderate conditions could obtain higher retention of nutritional composition in fresh -cut pumpkins.
摘要：
Ultrasound (US)-mediated sonodynamic therapy (SDT) has received extensive attention in pathogen elimination for non-invasiveness and high spatial and temporal accuracy. Considering that hydrogel can provide a healing-friendly environment for wounds, in this work, hybrid hydrogels are constructed by embedding Ag doped TiO(2) nanoparticles in chitosan-polyvinyl alcohol hydrogels for enhanced sonodynamic antibacterial therapy. With metal silver doped, TiO(2) nanoparticles sonosensitivity is improved to generate more reactive oxygen species (ROS), which endows hybrid hydrogels with high-efficient antibacterial properties. In vivo results show that hybrid hydrogel dressing can prevent infection and promote wound closure within 2days. The healing ratio excess 95% with no pus produced at the end of treatment. The therapeutic mechanism was identified that heterojunction formed in Ag doped TiO(2) facilitates the separation of charge carriers under US irradiation, leading to elevating ROS generation. The generated ROS promote hybrid hydrogels sonodynamic antibacterial therapeutic efficacy to thoroughly eliminate pathogen via disrupting bacterial cell membrane integrity, decreasing membrane fluidity and increasing membrane permeability. Besides, biofilm formation could be effectively inhibited. This work developed a hybrid hydrogel with amplified SDT effect for wound healing, which is expected to provide inspiration of hybrid hydrogels design and Ti-based nanomaterials sonosensitivity enhancement.
作者机构：
[Gong, Zhiyong; Li, Yuzhi; Liu, Xin; Fang, Bolong] College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China;[Fang, Bolong] Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, Wuhan Polytechnic University, Wuhan 430023, China. Electronic address: fangbl555@whpu.edu.cn;[Gong, Zhiyong; Li, Yuzhi; Liu, Xin] Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, Wuhan Polytechnic University, Wuhan 430023, China;[Lai, Weihua; Peng, Juan] State Key Laboratory of Food Science and Resources, School of Food Science and Technology, Nanchang University, Nanchang 330047, China;[Li, Yuzhi] Key Laboratory of Detection Technology of Focus Chemical Hazards in Animal-derived Food for State Market Regulation, Wuhan 430075, China
通讯机构：
[Fang, Bolong] K;Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, Wuhan Polytechnic University, Wuhan 430023, China. Electronic address:
关键词：
E. coli O157:H7;ELISA;Fluorescence enhancement;Fluorescence quenching;PCN-224
摘要：
In this study, a type of luminescent porous coordination network-224 (PCN-224) in alkaline conditions was synthesized with the dramatic fluorescence enhancement by 20.4 times, which was explained by the fact that the decrease of Zr(4+) content in alkaline conditions resulted in the partial recovery of the electron cloud density of 4,4',4'',4'''-(Porphine-5,10,15,20-tetrayl) tetrakis(benzoic acid) (TCPP). Given the large overlap between the excitation spectrum of PCN-224 and the absorption band of Ag nanoparticles (Ag NPs), the coating of the Ag layer on PCN-224 triggered the fluorescence quenching effect, which was applied to "turn off" fluorescence immunoassay for sensitive detection of Escherichia coli O157:H7 (E. coli O157:H7) in milk. The proposed immunoassay reached a low limit of detection (LOD) of 3.3×10(2) CFU mL(-1), 29.7 times more sensitive than the conventional ELISA. It will provide a novel alternative strategy for sensitively detecting pathogenic bacteria in the field of food safety.
作者机构：
[Qian, Shenxian; Chen, Zhenzhen; Chen, Can; Shi, Pengfei; Li, Yiwei] Department of Hematology, Affiliated Hangzhou First People's Hospital, West Lake University, School of Medicine, Hangzhou, China;[Li, TongYu] Department of Hematology, Ningbo First Hospital, Ningbo, China;[Li, Yun] Team of Neonatal & Infant Development, Health and Nutrition, NDHN, School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, China;[Li, Yun] Kindstar Global Precision Medicine Institute, Wuhan, China;[Li, Yun] Department of Scientific Research Project, Wuhan Kindstar Medical Laboratory Co., Ltd., Wuhan, China
通讯机构：
[Yun Li] T;[Shenxian Qian] D;Department of Hematology, Affiliated Hangzhou First People's Hospital, West Lake University, School of Medicine, Hangzhou, China<&wdkj&>Team of Neonatal & Infant Development, Health and Nutrition, NDHN, School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, China<&wdkj&>Kindstar Global Precision Medicine Institute, Wuhan, China<&wdkj&>Department of Scientific Research Project, Wuhan Kindstar Medical Laboratory Co., Ltd., Wuhan, China
关键词：
B cell immune;Biomarker;DLBCL;GPX4;Single cells
摘要：
At present, GPX4's role in the occurrence and development of diffuse large B lymphoma (DLBCL) is rarely reported. This study's purpose is to explore GPX4's significance in the diagnosis, treatment, and pathological mechanisms of DLBCL. The TIMER 2.0, GEPIA, and GEO databases were used to analyze GPX4's expression levels in DLBCL tissue, peripheral blood, and single cells, and evaluate its potential performance as a therapeutic and diagnostic marker. Cell experiments validate GPX4's role in DLBCL cells. And revealed the potential mechanism of GPX4's action from three aspects: immunity, pathogenic gene expression, and protein interaction. The results indicate that GPX4 can be used as a biomarker for treatment and diagnosis (FC>1.5, P<0.05, AUC>0.8, KM-P value<0.05). In single cell data, GPX4 also showed high expression in immune cells. Besides, cell experiments have confirmed that GPX4's high expression can inhibit DLBCL cells' proliferation. Meanwhile, we found a negative correlation between GPX4 and the 16 core DLBCL's pathogenic genes, and a significant negative correlation with immune B cell infiltration. In summary, GPX4 can serve as a potential therapeutic and diagnostic marker for DLBCL. GPX4's high expression can lead to a good prognosis in DLBCL patients, which may be related to its inhibition of cancer cell proliferation, high expression of key pathogenic genes, and infiltration of immune B cells.
