ε-聚赖氨酸盐酸盐对冷鲜草鱼制品中优势腐败菌细胞膜的作用机理研究
作者：
刘伟;向思颖;王宏勋;侯温甫
作者机构：
武汉轻工大学食品科学与工程学院,湖北武汉430023;湖北省生鲜食品工程技术研究中心,湖北武汉430023
会议名称：
第四届全国大宗淡水鱼加工技术与产业发展研讨会
会议时间：
20160917
会议地点：
武汉
会议论文集名称：
第四届全国大宗淡水鱼加工技术与产业发展研讨会论文集
关键词：
ε-聚赖氨酸盐酸盐;腐败菌;细胞膜;冷鲜草鱼;抑菌机理
摘要：
本文以冷鲜草鱼制品中典型的腐败菌为作用对象，探究ε-聚赖氨酸盐酸盐对冷鲜草鱼制品中微生物的细胞膜的作用机理。实验结果表明，ε-聚赖氨酸盐酸盐对假单胞菌、热杀索丝菌、腐败希瓦氏菌及乳酸菌的细胞膜通透性有显著影响，造成了细胞内Ca2+、K+、Mg2+的泄漏。
语种：
中文
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硒唑类衍生物的设计、合成与抗炎活性研究
作者：
乔俊飞;连振凯;蒋臣臣;黄思佳;马蓉蓉;...
作者机构：
武汉轻工大学化学与环境工程学院应用化学系,武汉,430023
会议名称：
第十一届全国化学生物学学术会议
会议时间：
20191118
会议地点：
广州
会议论文集名称：
第十一届全国化学生物学学术会议论文集
关键词：
硒唑;抑制剂;抗炎
摘要：
研究表明许多慢性炎症中存在组成型激活的NF-κB,抑制NF-κB 能减少炎症因子的分泌能够达到抗炎的效果。目前,基于NF-κB 信号通路已经成为抗炎药物开发的新靶点。
语种：
中文
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Research on Public Spaces Reconstruction of Old Communities under the Concept of Micro-renewal
作者：
Xiang Duan;Tai-Meng Xu;Chen-Hao Duan
期刊：
E3S Web of Conferences ,2021年237:04004-null ISSN：2267-1242
通讯作者：
Duan, X.
作者机构：
[Duan X.; Xu T.-M.] School of Engineering and Architecture, Wuhan Ploytechnic University, Wuhan, China;[Duan C.-H.] School of Architecture, Southeast University, Nanjing, China
通讯机构：
[Duan, X.] S;School of Engineering and Architecture, China
会议名称：
3rd International Symposium on Architecture Research Frontiers and Ecological Environment, ARFEE 2020
会议时间：
18 December 2020 through 20 December 2020
摘要：
Community public space is an important place for daily communication between residents. In the context of the current historical change of "urban double repairs", community renewal has raised new heights, and public space renewal has shifted from the surface of the physical space transformation layer to the protection, inheritance and environmental quality of the community. This article summarizes the concept and principles of micro-renewal, analyzes the related influencing factors of the reconstruction of the old community public space, further proposes the strategy of reconstructing the old community public space and enumerate several typical public space renewal schemes in old communities under the micro-renewal concept. © The Authors, published by EDP Sciences, 2021.
语种：
英文
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A hybrid KA-MoM method for scattering from dielectric target above a dielectric rough surface
作者：
Li, L.*
作者机构：
[Li, L.] Wuhan Polytech Univ, Sch Elect & Elect Engn, Wuhan 430048, Hubei, Peoples R China.
会议名称：
International Conference on Optoelectronic Materials and Devices (ICOMD)
会议时间：
DEC 18-20, 2020
会议地点：
Guangzhou, PEOPLES R CHINA
会议主办单位：
[Li, L.] Wuhan Polytech Univ, Sch Elect & Elect Engn, Wuhan 430048, Hubei, Peoples R China.
会议论文集名称：
Proceedings of SPIE
关键词：
Composite scattering;hybrid method;target above the rough surface;KA-MoM method
摘要：
By combining Kirchhoff approximation (KA) with the method of moment (MOM), a hybrid method is proposed to solve the scattering problem of a dielectric target above a dielectric rough surface. The field on the rough surface can be represented by the tapered incident wave and the scattering field of the target as the secondary incident wave using Kirchhoff approximation. Then, the electric field integral equations of the induced current and magnetic current on the target are derived to solve the problem. The field of the rough surface can be directly obtained by the tapered incident wave and the current and magnetic current on the target surface. Therefore, compared with the traditional method of moments, the hybrid algorithm only needs to solve the electric field integral equation of the target. The simulation of bistatic scattering from a cylindrical target on a Gaussian rough surface is carried out, and the effectiveness and effectiveness of the method are verified.
语种：
英文
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ICPM: An Intelligent Compound Prediction Model Based on GA and GRNN
作者：
Chen F.;Zhang C.
作者机构：
[Zhang C.; Chen F.] School of Mathematics and Computer Science, Wuhan Polytechnic University, Wuhan, 430000, China
会议名称：
7th International Conference on Artificial Intelligence and Security, ICAIS 2021
会议时间：
19 July 2021 through 23 July 2021
关键词：
Generalized regression neural network;Genetic Algorithm;Heavy metal content prediction;Parameter optimization;Small sample prediction
摘要：
In order to reduce the prediction error of the heavy metal content of farmland soil by General Regression Neural Network (GRNN), an Intelligent Compound Prediction Model (ICPM) was proposed. As the result of Genetic Algorithm optimization is good or bad, it mainly depends on whether it can guarantee the diversity of the population in the optimization process. Based on this, an Improved Genetic Algorithm (IGA) is proposed. IGA introduces the probability adjustment of the sine function transformation and the better gene replacement criterion into the Genetic Algorithm (GA). In the process of IGA’s optimization, the crossover probability and mutation probability continue to increase, ensuring the continuity of the diversity of the population. ICPM is a combined forecasting model of GRNN and IGA. It optimizes the smoothing factor of GRNN through IGA. The process of repeated optimization of IGA is also the process of repeated learning of existing knowledge by GRNN. ICPM not only ensures the continuous optimization of the population, but also the diversity of the population. Combined with the simulation prediction of the content of heavy metals Cr, Cu and Pb in farmland soil in Dongxihu District of Wuhan City, it proved that ICPM has better prediction performance and better generalization performance than GRNN and other models. © 2021, Springer Nature Switzerland AG.
语种：
英文
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The Study of Wind Turbine Blade Fatigue Test
作者：
Jiaqi Zheng;Qiming Yu;Botao Zhu;Changqing Wu;Yiling Huang;...
期刊：
E3S Web of Conferences ,2021年293:02024-null ISSN：2267-1242
通讯作者：
Zheng, J.
作者机构：
[Yu Q.; Zheng J.; Wu M.; Shanqing He; Wu C.; Zhu B.; Huang Y.] Civil engineering and architectural institute, Wuhan Polytechnic University, Hubei, Wuhan, 430023, China;[He S.] Wuhan Zhongjiao Qingchuan road and Bridge Technology Co, Ltd, Hubei, Wuhan, 430050, China
通讯机构：
[Zheng, J.] C;Civil engineering and architectural institute, Hubei, China
会议名称：
3rd Global Conference on Ecological Environment and Civil Engineering, GCEECE 2021
会议时间：
25 June 2021 through 27 June 2021
摘要：
The fatigue test of wind turbine blade is an important means to verify the fatigue life of wind turbine blade. This paper analyses the problems existing in the fatigue test of wind turbine blade, focuses on the excitation mode, the relationship between excitation amplitude and vibration frequency and the vibration principle, and puts forward feasible solutions in practical operation. © The Authors.
语种：
英文
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Analysis of water saving in the construction process based on green building
作者：
Shiyao Wu;Zuxu Zou;Shuyi Fan
期刊：
E3S Web of Conferences ,2021年272:01006-null ISSN：2267-1242
通讯作者：
Zou, Z.
作者机构：
[Zou Z.; Fan S.; Wu S.] Civil Engineering and Architectural Institute, Wuhan Polytechnic University, Hubei, Wuhan, China
通讯机构：
[Zou, Z.] C;Civil Engineering and Architectural Institute, Hubei, China
会议名称：
2021 International Conference on Environmental Pollution and Governance, ICEPG 2021
会议时间：
21 May 2021 through 23 May 2021
摘要：
Under the premise of ensuring their own food, clothing, housing and transportation, We aim for sustainable development to make the building and nature coordinate with each other and to create a healthier and more comfortable living space. This article analyzes the water resources in the construction process, discusses why they are wasted and how to reduce their waste In addition, a water-saving combination method will be proposed to optimize this situation. © The Authors, published by EDP Sciences, 2021.
语种：
英文
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Multi-dimensional Visualization and Simulation Analysis of COVID-19 Outbreak
作者：
Zeng W.;Zhang Y.G.;Hu K.;Jiang Y.X.
作者机构：
[Hu K.; Jiang Y.X.; Zeng W.; Zhang Y.G.] Department of Electrical and Electronic Engineering, Wuhan Polytechnic University, Hubei, 430023, China
会议名称：
7th International Conference on Artificial Intelligence and Security, ICAIS 2021
会议时间：
19 July 2021 through 23 July 2021
关键词：
COVID-19;Echarts;Java Swing;SEIR model prediction;Visualization
摘要：
The progression of the global COVID-19 epidemic situation is the main focus of attention of all countries in the world. Due to characteristics, such as multi-origins, huge amount, and inaccessibility, of the existing data, an all-round analyzation of the epidemic situation, which is in dire need, is impeded. The aim of the following study is to provide a multi-dimensional analysis of COVID-19 through visualization and dynamic simulation of data. In order to achieve this goal, the study collected related data though multiple platforms and used tools such as Echarts and Java Swing to visualize the data, and then dynamically simulated the transmission model. Moreover, the data of Wuhan has been applied to the SEIR model to study the effect of quarantine on the transmission of COVID-19. Ultimately, the study hopes to demonstrate an effective method of data analyzation that can be applied to prevent and contain similar outbreak in the future. © 2021, Springer Nature Switzerland AG.
语种：
英文
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Blockchain technology in food safety governance
作者：
Wang, Puqing;Yang, Saidi;Li, Teng
作者机构：
[Wang, Puqing; Yang, Saidi] College of Management, Wuhan Polytechnic University, Wuhan, China;[Li, Teng] College of Economics and Management, Huazhong Agricultural University, Wuhan, China
会议名称：
12th International Conference on E-business, Management and Economics, ICEME 2021
会议时间：
July 17, 2021 - July 19, 2021
会议地点：
Virtual, Online, China
摘要：
The blockchain technology is widely considered as 'a symbol of the Fourth Industrial Revolution'. As a 'machine of trust', blockchain can contribute to safer food supply chains, the key of which is the trust between food producers and consumers. The paper presents case studies to examine the effectiveness and advantages of applying blockchain technology in food safety governance, as well as its influences on all related participants in the supply chain. This study demonstrates that there is great potential for the blockchain technology, due to its unique characteristics of openness, transparency, sharing and security, to improve the food safety governance. © 2021 ACM.
语种：
英文
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Research and Application of the Standard Formulation Technology for Three Kinds of Specialized Raw Materials
作者：
Zhou S.;Liu J.;Zhou K.;Zhou J.;Li G.
作者机构：
[Zhou K.; Zhou S.; Liu J.] College of Mathematics and Computer Science, Wuhan Polytechnic University, Wuhan, 430023, China;[Zhou J.] College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, 430023, China;[Li G.] Enterprise School Joint Innovation Center, Qianjiang Jujin Rice Industry Co., Ltd., Hubei, China
会议名称：
15th International Conference on Bio-Inspired Computing: Theories and Applications, BIC-TA 2020
会议时间：
23 October 2020 through 25 October 2020
关键词：
Information entropy theory;Multi-objective optimization model;Prediction equations;Residual analysis;Specialized raw material
摘要：
There are many raw materials for the production of rice wine, rice flour and glutinous rice products, It is one of the problems to be solved at present to select the appropriate proportion of raw materials for the production of these three products. In order to solve this problem, this paper finds out the specialized raw material index standard of the above three products through data analysis method and multi-objective optimization under the condition of meeting the various characteristic demands of the three products, so as to guide the production of enterprises. Based on the index data of three raw materials and products obtained from experimental tests, firstly, the data was standardized, and the noise of sample data was eliminated through residual analysis. Secondly, the optimal prediction equation was found by comparing various prediction methods, so as to make the formulation of raw materials more effective. Finally, a multi-objective optimization model is established. According to the contribution rate of the raw material index of the three products, the information entropy method is used to calculate the weight of the raw material index corresponding to the three products, so that the final solution of the specific raw material index range is more accurate, effective and reasonable. © 2021, Springer Nature Singapore Pte Ltd.
语种：
英文
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HPLC法测定黄连解毒汤中小檗碱和黄岑苷的含量
作者：
王慧;熊春红;李睿智;叶纯;吴仲元;...
作者机构：
武汉轻工大学动物科学与营养工程学院,武汉,430023
会议名称：
中国畜牧兽医学会兽医药理毒理学分会第十四次学术讨论会
会议时间：
20171017
会议地点：
青岛
会议论文集名称：
中国畜牧兽医学会兽医药理毒理学分会第十四次学术讨论会论文集
关键词：
黄连解毒汤;小檗碱;黄芩苷;HPLC法
摘要：
[目的]建立一种测定黄连解毒汤中2 种主要活性成分小檗碱和黄芩苷含量测定的HPLC 定量方法,用于黄连解毒汤浸膏及制剂的质量控制.[方法]Waters 高效液相色谱系统(1525 泵,2489紫外检测器),Diamonsil C18色谱柱(250×4.6 mm,5μm)；以0.46％甲酸一0.3％三乙胺的乙腈溶液(A)和0.46％甲酸一0.3％三乙胺的水溶液(B)为流动相；梯度洗脱：0-37min,A从10％线性升至32％,37-45 min,A 从32％线性升至50％保持5 min 后,将梯度变回A 为10％,再保持5 min；检测波长：260 nm；流速：1.0mL/min；柱温：30℃.
语种：
中文
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Study on the morphology and temperature-sensitivity of poly(N-isopropylacrylamide-co-acrylic acid) microgel by atomic force microscope
作者：
Xiao Wang;Yana Li;Guoquan Zhang;Beihai Wang
期刊：
E3S Web of Conferences ,2021年267:02007-null ISSN：2267-1242
通讯作者：
Li, Y.
作者机构：
[Wang X.; Wang B.; Zhang G.; Li Y.] Wuhan Polytechnic University, Wuhan, 430023, China
通讯机构：
[Li, Y.] W;Wuhan Polytechnic UniversityChina
会议名称：
7th International Conference on Energy Science and Chemical Engineering, ICESCE 2021
会议时间：
21 May 2021 through 23 May 2021
摘要：
A group of crosslinked poly(N-isopropylacrylamide-co-acrylic acid) (P(NIPAM-co-AAc)) microgels with different amount of acrylic acid (AAc) were synthesized by surfactant-free emulsion polymerization. The morphology and environmental sensitivity were studied by atomic force microscope (AFM). It was founded that the microgel particles were monodispersed and the diameter of the dried microgel latex particles was about 800nm. Moreover, the volume of the microgel particles decreased as the environmental temperature increased from 15C to 50C, which revealed the temperature-sensitivity of P(NIPAM-co-AAc)) microgel. © The Authors, published by EDP Sciences, 2021.
语种：
英文
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基于耦合协调度理论的文化消费环境测评研究--以武汉市为例
作者：
张国超;唐培
作者机构：
武汉轻工大学,艺术与传媒学院,湖北武汉,430023
会议名称：
2017第十四届中国文化产业新年论坛
会议时间：
20170107
会议地点：
北京
会议论文集名称：
2017第十四届中国文化产业新年论坛论文集
关键词：
文化消费环境;模糊综合评价;指标体系;耦合协调度
摘要：
耦合协调度理论提供了一条文化消费环境研究的独特路径.在该理论的指导下,本研究构建了文化消费环境评价的指标体系,以武汉市为例演示了模糊综合评价法及耦合协调度计算的过程.研究结果与质性研究相一致,表明该路径具有一定的科学性和有效性.此外,探讨了测评方法的优势、不足及优化思路.
语种：
中文
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Research on the development of green buildings under the high-speed development mode
作者：
Shihan Zhu;Jingjing Liu;Chenjie Wu
期刊：
E3S Web of Conferences ,2021年272:01021-null ISSN：2267-1242
通讯作者：
Liu, J.
作者机构：
[Wu C.; Zhu S.] School of Civil Engineering and Architecture, Wuhan Polytechnic University, China;No. xsky2021146, Wuhan Polytechnic University, China;[Liu J.] School of Civil Engineering and Architecture, Wuhan Polytechnic University, China, Wuhan Polytechnic University, No. xsky2021146, China
通讯机构：
[Liu, J.] S;School of Civil Engineering and Architecture, China
会议名称：
2021 International Conference on Environmental Pollution and Governance, ICEPG 2021
会议时间：
21 May 2021 through 23 May 2021
摘要：
In the rapid development of science and technology today, environmental problems are becoming more and more serious; the construction industry as the first high energy-consuming field with human production and life, the promotion of green buildings is of great significance. Under the high-speed development mode, how to make green buildings develop steadily has become a problem to be solved by various industries in China. Through the literature research method and the social investigation method, this paper analyses the development constraints of green buildings from the perspective of the four participants of market economy: government, real estate enterprises, consumers and practitioners. Respectively put forward some suggestions such as policy regulation, enterprise norms, green talents cultivation and collect feedback. © The Authors, published by EDP Sciences, 2021.
语种：
英文
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Research on Wuhan Sponge City Construction and Water Resumption System
作者：
Hongmin Li;Lu Yan
期刊：
E3S Web of Conferences ,2020年143:01038- ISSN：2267-1242
通讯作者：
Li, H.
作者机构：
[Li H.] School of Civil Engineering and Architecture, Wuhan Polytechnic University, Wuhan, China;[Yan L.] Hubei Audit Office, Wuhan, China
通讯机构：
[Li, H.] S;School of Civil Engineering and Architecture, China
会议名称：
2nd International Symposium on Architecture Research Frontiers and Ecological Environment, ARFEE 2019
会议时间：
20 December 2019 through 22 December 2019
摘要：
Pocket parks are small public spaces between building entities which are available to the public. It can be located in a city, suburb, or country. It has a small area and is relatively flexible. According to the characteristics of China's cities, this article puts forward methods and measures for the landscape reconstruction design of existing urban communities in China. The thesis takes a Residential area of a certain district in Wuhan as a design case. It discusses how to combine the landscape design of the existing community with the rainwater problem, the goals, principles and measures of the landscape reconstruction design of the existing community based on the sponge city concept are proposed. Using rainwater as a resource to collect, purify, store, and recycle, alleviate water shortages and urban flood disasters, and it summarizes the methods and strategies for landscape reconstruction design of existing communities. Finally, according to the problems in the residential area and the actual needs of residents, combined with the current conditions of the site, following the design principles of people-oriented and local conditions, the use of sponge city technology measures on the green landscape, roads and parking lots, rooftop landscape of underground garages, plants Matching and other specific reconstruction designs provide theoretical and design references for the application of the "sponge city" concept in the design of urban residential quarters. © The Authors, published by EDP Sciences, 2020.
语种：
英文
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Structure design and kinematics analysis of lower limb rehabilitation training robot
作者：
Hu, Ao;Yin, Qiang;Li, Qian;Yang, Hongjun;Zhang, Yonglin
作者机构：
[Hu, Ao; Yin, Qiang; Li, Qian; Yang, Hongjun; Zhang, Yonglin] School of Mechanical Engineering, Wuhan Polytechnic University, Wuhan, China
会议名称：
3rd International Conference on E-Business, Information Management and Computer Science, EBIMCS 2020
会议时间：
December 5, 2020 - December 6, 2020
会议地点：
Wuhan, China
摘要：
In this article, a new type of lower limb rehabilitation training robot is designed and related rehabilitation training programs are proposed. The robot system uses a bionic lower limb movement mechanism to drive the hip and ankle joints of the human body, and is combined with a treadmill-type vertical vibration training device to simulate human walking for lower limb rehabilitation training. The overall structure is modeled in three dimensions. In addition, the simplified link mechanism model is used to analyze the robot's motion process by analytical methods, and the motion equations and motion parameters of each component are derived. Meanwhile, simulation software was used to simulate the motion speed of the thigh link and the calf link in the bionic lower limb motion mechanism, and the motion angle range of the hip and knee joints was also simulated. According to the simulation results, the rehabilitation training program is verified and revised to further optimize the design. It lays a theoretical foundation for the movement of the lower limb rehabilitation training robot in the future, and has certain research significance. © 2020 ACM.
语种：
英文
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Simulation of Separation of Rice Bran and Broken Rice in Top-suction Rice Mill with Annular Duct
作者：
Lizong, Wang;Nanhui, Yu;Jijun, Fan;Yonglin, Zhang;Houchang, Pei;...
作者机构：
[Lizong, Wang; Nanhui, Yu; Jijun, Fan; Yonglin, Zhang; Houchang, Pei; Yifan, Zhang] College of Mechanical Engineering, Wuhan Polytechnic University, Wuhan, China
会议名称：
3rd International Conference on E-Business, Information Management and Computer Science, EBIMCS 2020
会议时间：
December 5, 2020 - December 6, 2020
会议地点：
Wuhan, China
摘要：
In view of the defect that the rice bran collected by the traditional rice milling machine will be mixed with broken rice, the top-suction rice mill with annular duct was designed to separate and collect the rice bran and broken rice in the annular duct. The DEM-CFD coupling simulation method and the sliding mesh method were used to simulate the movement of the motion of particles in the new rice mill. The results show that the fluid velocity in the annular duct is between the suspension velocity of rice bran and broken rice when the wind speed of the top suction outlet and the fan is 6 m/s and 10 m/s respectively. The rice bran floated up (the rising velocity is 1 ∼ 3 m/s) along the wall of the annular duct and was discharged from the top suction outlet under the updraft in the duct. Additionally, the partition can effectively prevent the rice bran from falling. The broken rice fell and was discharged from the bottom air inlet with the velocity of 1 m/s. The separation efficiency was 99.6%, which showed that the separation effect was excellent. © 2020 ACM.
语种：
英文
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Technology optimization study on high fragrance cold solubility instant powder of Hubei Qingzhuan tea produced by freeze-drying method
作者：
Hui-hui Li;Qing-qing Xie;Qing-mei Pan;Si-yun He;Hong-wei Sui
期刊：
E3S Web of Conferences ,2020年185:04030-null ISSN：2267-1242
作者机构：
[He S.-Y.; Li H.-H.; Xie Q.-Q.; Sui H.-W.] College of Food Technology, Wuhan Business University, Wuhan, China;[Pan Q.-M.] School of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, China
会议名称：
2020 International Conference on Energy, Environment and Bioengineering, ICEEB 2020
会议时间：
7 August 2020 through 9 August 2020
摘要：
Although Chinese instant tea powder production ranks first in the world, instant dark tea powder was rare in the market. Zhaoliqiao, Changshengchuan and Yangloudong Qingzhuan tea were used to optimize the technology process of high fragrance cold solubility instant powder while absorbance value was taken as detection index by single factor experiments combined with orthogonal experiments, which could provide a beneficial reference for deep utilization of Hubei Qingzhuan tea. The data showed the particle size of tea, tea-water ratio and extracting temperature significantly affected the extraction efficiency while extraction time did little. The extraction efficiency was the highest when using 120-mesh dark tea, extracting with 1:40 tea-water ratio, and extracting temperature of 100. © The Authors, published by EDP Sciences, 2020.
语种：
英文
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Information Flow-Based Security Construction for Compositional InterfaceAutomata
作者：
Mingdi Xu;Zhaoyang Jin;Fan Zhang;Feng Cui
期刊：
Communications in Computer and Information Science ,2020年 1149: 31-43 ISSN：1865-0929
通讯作者：
Zhang, F.
作者机构：
[Jin Z.; Cui F.; Xu M.] Wuhan Digital and Engineering Institute, Wuhan, Hubei 430205, China;[Zhang F.] School of Mathematics and Computer Science, Wuhan Polytechnic University, Wuhan, Hubei 430023, China
通讯机构：
[Zhang, F.] S;School of Mathematics and Computer Science, China
会议名称：
13th Chinese Conference on Trusted Computing and Information Security, CTCIS 2019
会议时间：
24 October 2019 through 27 October 2019
会议论文集名称：
Trusted Computing and Information Security
关键词：
Compositional security;Information flow;Noninterference
摘要：
Information flow has been considered as a critical requirement to solve security related issues for complicated component-based system. However, security conditions are often fragile and general security properties may be not available to enforce the composition. Thus, this paper gives the computation model of interface automata (IA) and studies how the compositional interfaces behave to capture the information leakage with security process algebra (SPA) language. And we find that persistent bisimulation-based non deducibility property is preserved under composition, while it is not fully applicable to IA model. So several sufficient conditions for the property is developed to apply for composition of interface automata. Those conditions are given as theorems and proved efficiently to analyze security. Finally, we cite a classical instance to handle the composition and use an automatic verification software to test the correctness of our algorithms on compositional conditions. © Springer Nature Singapore Pte Ltd 2020.
语种：
英文
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Molecular cloning and characterization of GbMECT and GbMECP gene promoters from Ginkgo biloba
作者：
Cheng Hua;Li Linling;Yuan Honghui;Xu Feng;Cheng Shuiyuan
作者机构：
Economic Forest Germplasm Improvement and Comprehensive Utilization of Resources of Hubei Key Labora;College of Horticulture and gardening, Yangtze University, Jingzhou 434025, China;school of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University,Wuhan 430023, Hubei,
会议名称：
全国第二十一次银杏学术研讨会
会议时间：
201511
会议地点：
山东郯城
会议论文集名称：
全国第二十一次银杏学术研讨会 论文集
关键词：
cis-acting elements;hormone;wounding;diterpenoids;terpene lactones;functional analysis
摘要：
G'inkgolides are key pharmaceutical components in Ginkgo biloba.According to the cDNA sequence of MECPs and MECT gene to design primer, and we obtained the two genes promoter from Ginkgo genomic DNA using genome walking method.In this study, the promoters of MECPs and MECT, with 744 bp and 982 bp in length.The ciselements of GbMECPs and GbMECT promoters were predicted and analyzed by the plant cis-acting regulatory element (CARE) database.We found some major cis-element in the sequence of GbMECT and GbMECPs promoters.The GbMECPs promoter has six TATA box, eight CAAT box;and the GbMECT has five TATA box, seven CAAT boxes.Furthermore, some cis-elements in the promoters of GbMECPs and GbMECT promoter,including hormone, light-regulated elements, UB-B induced-elements and stress-related dehydration-responsive.The qRT-PCR showed that the MECPs gene are mainly involved in responses to CCC and UV-B, and MECTs are mainly responses to wounding treatment,these results also showed the expression model were in line with the cis-element.During the annual growth cycle, the GbMECPs were significantly correlated with sesterpene accumulation in leaves.A fitted quadratic curve showed the best model for relating GbMECPs with sesterpene in leaves.These results will help us to understand the transcriptional regulatory mechanisms involved in key gene expression and ginkgolide accumulation in G.biloba.
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英文
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