摘要：
Our previous study revealed that green tea polysaccharide conjugate (gTPC) has emulsion effect, but its emulsifying ability is weak. In order to improve the emulsification ability of gTPC, gTPC and bovine serum albumin (BSA) were combined to form five different mass proportions of the TPC/BSA (TB) complex: TPC/BSA: 5:1, 5:2, 5:3, 5:4, and 5:5 w/w. We observed that the 5:5 w/w TB emulsion was more hydrophobic and surface-active. Furthermore, the emulsions prepared using 50.00wt% medium-chain triglycerides exhibited the best stability. In addition, the TB emulsion exhibited stability in adverse environments of pH, salt, and heat; in particular, under salt conditions, no significant changes were observed in zeta potential. Subsequently, in vitro simulated digestion experiments were performed to investigate the use of TB emulsions for β-carotene encapsulation. We observed that the encapsulation efficiency for β-carotene was approximately 90.0%; it was subsequently released in the intestine.
作者机构：
[Cao, Jie; Xu, Feng; Wang, Li-Na; Zhou, Xian; Zhang, Wei-wei; Ye, Jia-Bao] Yangtze Univ, Coll Hort & Gardening, Jingzhou, Peoples R China.;[Cheng, Shuiyuan; Liu, Xiao-Meng] Wuhan Polytech Univ, Natl R&D Ctr Se Rich Agr Prod Proc Technol, Sch Modern Ind Selenium Sci & Engn, Wuhan 430048, Peoples R China.;[Xu, Feng; Xu, F] Yangtze Univ, Coll Hort & Gardening, Jingzhou 434025, Hubei, Peoples R China.
通讯机构：
[Xu, F ] Y;Yangtze Univ, Coll Hort & Gardening, Jingzhou 434025, Hubei, Peoples R China.
摘要：
4-Coumarate-CoA ligase (4CL) gene plays vital roles in plant growth and development, especially the regulation of lignin metabolism and flavonoid synthesis. To investigate the potential function of 4CL in the lignin biosynthesis of Ginkgo biloba, this study identified two 4CL genes, Gb4CL1 and Gb4CL2, from G. biloba genome. Based on the phylogenetic tree analysis, Gb4CL1 and Gb4CL2 protein were classified into Class I, which has been confirmed to be involved in lignin biosynthesis. Therefore, it can be inferred that these two genes may also participate in lignin metabolism. The tissue-specific expression patterns of these two genes revealed that Gb4CL1 was highly expressed in microstrobilus, whereas Gb4CL2 was abundant in immature leaves. The onion transient expression assay indicated that Gb4CL1 was predominantly localized in the nucleus, indicating its potential involvement in nuclear functions, while Gb4CL2 was observed in the cell wall, suggesting its role in cell wall-related processes. Phytohormone response analysis revealed that the expression of both genes was upregulated in response to indole acetic acid, while methyl jasmonate suppressed it, gibberellin exhibited opposite effects on these genes. Furthermore, Gb4CL1 and Gb4CL2 expressed in all tissues containing lignin that showed a positive correlation with lignin content. Thus, these findings suggest that Gb4CL1 and Gb4CL2 are likely involved in lignin biosynthesis. Gb4CL1 and Gb4CL2 target proteins were successfully induced in Escherichia coli BL21 with molecular weights of 85.5 and 89.2kDa, proving the integrity of target proteins. Our findings provided a basis for revealing that Gb4CL participated in lignin synthesis in G. biloba.
摘要：
The production and sales of electric vehicle lithium-ion batteries (EV LIB) have experienced rapid growth in the past decade, resulting in an extensive and intricate supply chain network (SCN). The SCNs orchestrated by different focal firms are interdependent due to the joint suppliers and customers. This interdependence, coupled with geographical segmentation, introduces vulnerability to the global EV LIB SCN. Focal firms need management insights from the multiplexity of the global EV LIB SCN to address the potential vulnerability. This investigation utilizes the empirical data regarding the supplier-buyer relationships of the top six EV LIB manufacturers in 2020 to construct the multilayered EV LIB SCN. Then, a focal firm-specific analysis is conducted to explore the multiplexity of the global EV LIB SCN, including identifying the influential firms, measuring the structure of SCNs led by different focal firms, and distinguishing the dependence and discrepancies of different focal firms' SCNs. Furthermore, an SCN vulnerability (SCNV) index was designed to assess the vulnerability of such a multilayered SCN in two disruption scenarios, i.e., single-country blockade and intercountry blockade. The structural analysis can assist focal firms in allocating vulnerability-coping resources more effectively. Besides, the assessment of SCNV can assist managers in identifying potential risk sources beyond their own SCNs.
摘要：
The use of microalgae-bacteria consortia (MBC) for wastewater treatment has garnered attention as their interactions impart greater environmental adaptability and stability compared with that obtained by only microalgae or bacteria use, thereby improving the efficiency of pollutant removal and bio-product productivity. Additionally, the value-added bio-products produced via biorefineries can improve economic competitiveness and environmental sustainability. Therefore, this review focuses on the interaction between microalgae and bacteria that leads to nutrient exchange, gene transfer and signal transduction to comprehensively understand the interaction mechanisms underlying their strong adaptability. In addition, it includes recent research in which MBC has been efficiently used to treat various wastewater. Moreover, the review summarizes the use of MBC-produced biomass in a biorefining context to produce biofuel, biomaterial, high-value bio-products and bio-fertilizer. Overall, more effort is needed to identify the symbiotic mechanism in MBC to provide a foundation for circular bio-economy and environmentally friendly development programmes.
摘要：
In the realm of practical problem-solving, multi-objective optimisation problems with redundant variables and indefinite objective functions (MOPRVIF) are becoming increasingly prevalent. MOPRVIF involve determining the optimal decision variables that optimise multiple objectives, leveraging the relational data of a set of variables and multiple objectives. For these problems, this paper focuses on the following two issues: one is the demand for a unified computational model to solve this problem; the other is how to improve the algorithm's deep intelligent search capability. In this regard, this paper designs a dual data-driven multi-objective optimisation method. The method used consisted of four parts: elimination of redundant variables (ERV), objective function construction (OFC), selection evolution operator (SEO), and multi-objective evolutionary algorithm (MOEA). MOEA was the main focus of the method. ERV is data preparation and variable selection according to multiple objectives. OFC involves constructing the relationship model between variables and objectives, and a high-accuracy model is important for guaranteeing reliable results. Furthermore, SEO can adjust the evolution operator during a deep search. This is an important guarantee for deep, intelligent search. MOEA combined OFC and SEO to form the final solution algorithm-Dual Data Driven Multi-Objective Evolutionary Algorithm (DDMOEA). DDMOEA was explored using two different disciplinary problems of drug compound optimisation and wild blueberry cultivation and benchmarks were selected. The first two problem domains are distinct. The first problem is more complex than the second; however, both encompass redundant variables and indefinite objective functions. Benchmarks are utilised independently to gauge the profound intelligent search capability. The experiments affirm that the dual data -driven optimization approach proposed in this paper is effective, practical, and scalable.
摘要：
Four typical forest types in Sygera Mountain, namely, Pinus armandii (PA), Picea likiangensis var. linzhiensis (PLL), Abies georgei var. smithii (AGS), and Juniperus saltuaria (JS) were considered using methods such as analysis of variance and stoichiometry. Effects of changes of nutrient content and stoichiometric ratio of various organs of arbor plants were evaluated. Compared with global plant nutrients, this study area had lower N and P contents. The growth of the Juniperus saltuaria forest was jointly restricted by N and P, and as the environmental gradient decreased, the growth of the other three forest types changed from N and P joint restrictions to N restrictions. The distribution order of element content in various organs among different forest types was consistent as follows: leaf>branch>root>trunk. There was a strong domestication reaction between N and P contents. This led to a significantly higher C/N ratio of the square branch Berlin compared to the other three forest types, while the C/P and N/P ratios were opposite. This may be due to the "optimal allocation principle" of nutrients in various organs under different environmental conditions. The nutrient allocation patterns of plant organs in different forest types were not the same, which reflects the differences in life strategies and nutrient utilization among different forest species.
摘要：
As an advanced time-frequency (TF) decomposition (TFD) method, variational nonlinear chirplet mode decomposition (VNCMD) decomposes the original signal into a series of nonlinear chirplet modes (NCMs), such that the inherent characteristic information contained in the signal can be revealed effectively. However, the decomposition ability of VNCMD is largely affected by the prior instantaneous frequency (IF) and the pre-set parameters. In practical engineering applications, the presence of noise and interference components often complicates the accurate determination of prior IFs and appropriate decomposition parameters. Considering the above issues, in order to precisely extract the NCMs and realize the effective analysis of mechanical vibration signals, this paper mainly focuses on the drawbacks of accurate prior IF and the decomposition parameters of VNCMD, and proposed an improved version via local maximum synchrosqueezing transform and a recursive mode extracting scheme. The performance of the proposed method is evaluated through simulation cases, and the results demonstrate its effectiveness. Finally, the proposed method is successfully applied to bearing data analysis and rub-impact fault detection.
摘要：
Two-dimensional transition metal disulfides (TMDs) heterojunction is a very promising non-precious metal catalyst that has been widely used as a catalyst for hydrogen evolution reactions. In this paper, the effects of different positions of Se vacancies on the electronic structure and hydrogen evolution reaction electrocatalyst of MoSe2/WSe2 heterojunction are investigated by first-principles calculations. After the introduction of vacancy, defect states appear between the conduction band bottom and the Fermi energy level of the heterojunction. This is more favorable for the electron transfer between H and the substrate. The results of the density of states calculations indicate that the defect states appear because the d orbitals of the transition metal atoms containing the Se vacancy layer hybridize with the p orbitals of the Se atoms. The differential charge density indicates that the Se vacancies promote the charge transfer of MoSe2/WSe2. In addition, calculations of the Gibbs free energy for the hydrogen evolution reaction of heterojunctions show that Se vacancies (especially the outer vacancies) enhance the electrocatalytic hydrogen precipitation performance of heterojunctions. The results provide a new way to regulate the hydrogen evolution properties of MoSe2/WSe2 heterojunctions.
摘要：
To improve the emulsifying property of sodium caseinate (NaCas) as stabilizer of oil-in-water emulsions and encapsulation of bioactive compounds, three hexaglycerol mono-fatty acid esters were chosen as small molecular weight surfactants to complex with NaCas. Hexaglycerol monooleate (HGMO) was found to be the optimal surfactant and the optimal mass ratio was 1:1, through characterization of particle size, Zeta-potential, and turbidity. Fluorescence and FTIR spectra indicated that the hydrophobic interaction and hydrogen bond provided driving forces to the formation of stable complex. The complexation of HGMO to NaCas increased the surface hydrophobicity and decreased surface tension compared with NaCas, and strengthened the EAI and ESI. The NaCas-HGMO complex had good stabilization on rice bran oil-in-water emulsions, in a wide pH and ionic strength, and the forwarding Cur encapsulation in O/W emulsions dramatically reduced the degradation during storage at 4celcius. Therefore, the present NaCas-HGMO complex might be employed as an effective emulsifier to stable O/W emulsions that load lipophilic bioactives in functional foods or beverages.
作者机构：
[Zhang, Qi; Ge, Feng; Yang, Mingkun; Zhao, Jindong; Jia, Kun; Liu, Xin; Cao, Gaoxiang] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China.;[Zhang, Qi; Ge, Feng; Yang, Mingkun; Jia, Kun; Cao, Gaoxiang] Univ Chinese Acad Sci, Coll Adv Agr Sci, Beijing 100049, Peoples R China.;[Liu, Xin] Wuhan Polytech Univ, Sch Anim Sci & Nutr Engn, Wuhan 430070, Peoples R China.;[Zhao, Jindong] Peking Univ, Coll Life Sci, State Key Lab Prot & Plant Genet Engn, Beijing 100871, Peoples R China.
通讯机构：
[Ge, F; Zhao, JD ] C;[Ge, F ] U;Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China.;Univ Chinese Acad Sci, Coll Adv Agr Sci, Beijing 100049, Peoples R China.;Peking Univ, Coll Life Sci, State Key Lab Prot & Plant Genet Engn, Beijing 100871, Peoples R China.
摘要：
Lysine acetylation is a conserved regulatory posttranslational protein modification that is performed by lysine acetyltransferases (KATs). By catalyzing the transfer of acetyl groups to substrate proteins, KATs play critical regulatory roles in all domains of life; however, no KATs have yet been identified in cyanobacteria. Here, we tested all predicted KATs in the cyanobacterium Synechococcus sp. PCC 7002 (Syn7002) and demonstrated that A1596, which we named cyanobacterial Gcn5-related N-acetyltransferase (cGNAT2), can catalyze lysine acetylation in vivo and in vitro. Eight amino acid residues were identified as the key residues in the putative active site of cGNAT2, as indicated by structural simulation and site-directed mutagenesis. The loss of cGNAT2 altered both growth and photosynthetic electron transport in Syn7002. In addition, quantitative analysis of the lysine acetylome identified 548 endogenous substrates of cGNAT2 in Syn7002. We further demonstrated that cGNAT2 can acetylate NAD(P)H dehydrogenase J (NdhJ) in vivo and in vitro, with the inability to acetylate K89 residues, thus decreasing NdhJ activity and affecting both growth and electron transport in Syn7002. In summary, this study identified a KAT in cyanobacteria and revealed that cGNAT2 regulates growth and photosynthesis in Syn7002 through an acetylation-mediated mechanism. The cyanobacterial Gcn5-related N-acetyltransferase regulates the growth and photosynthesis of Synechococcus PCC 7002 through an acetylation-mediated mechanism.
摘要：
Although it is widely accepted that LTBI transitioning to ATB results from a disruption of host immune balance and a weakening of protective immune responses, the exact underlying immunological mechanisms that promote this conversion are not well characterized. This review systematically explores the immunoregulatory mechanisms in LTBI and ATB, along with their practical applications. Abstract Nearly one‐fourth of the global population is infected by Mycobacterium tuberculosis (Mtb), and approximately 90%–95% remain asymptomatic as latent tuberculosis infection (LTBI), an estimated 5%–10% of those with latent infections will eventually progress to active tuberculosis (ATB). Although it is widely accepted that LTBI transitioning to ATB results from a disruption of host immune balance and a weakening of protective immune responses, the exact underlying immunological mechanisms that promote this conversion are not well characterized. Thus, it is difficult to accurately predict tuberculosis (TB) progression in advance, leaving the LTBI population as a significant threat to TB prevention and control. This article systematically explores three aspects related to the immunoregulatory mechanisms and translational research about LTBI: (1) the distinct immunocytological characteristics of LTBI and ATB, (2) LTBI diagnostic markers discovery related to host anti‐TB immunity and metabolic pathways, and (3) vaccine development focus on LTBI. This article is categorized under: Infectious Diseases > Molecular and Cellular Physiology Infectious Diseases > Genetics/Genomics/Epigenetics Immune System Diseases > Genetics/Genomics/Epigenetics
期刊：
Journal of Cleaner Production,2024年435:140388 ISSN：0959-6526
通讯作者：
Zhou, J;Zhang, WJ;Ning, XP
作者机构：
[Zhou, Ju; Zhou, J] Luoyang Normal Univ, Business Sch, Luoyang 471934, Peoples R China.;[Xu, Ning] Henan Normal Univ, Sch Polit Sci & Publ Adm, Xinxiang 453007, Peoples R China.;[Zhang, WJ; Zhang, Wenjie] Wuhan Polytech Univ, Sch Management, Wuhan 430048, Peoples R China.;[Ning, XP; Ning, Xueping] Shanghai Univ Int Business & Econ, Sch Int Business, Shanghai 201620, Peoples R China.
通讯机构：
[Zhang, WJ ] W;[Ning, XP ] S;[Zhou, J ] L;Luoyang Normal Univ, Business Sch, Luoyang 471934, Peoples R China.;Wuhan Polytech Univ, Sch Management, Wuhan 430048, Peoples R China.
关键词：
New-type urbanization pilot policy;Agricultural low-carbon development;Agricultural technological efficiency;Difference-in-differences model
摘要：
Advancing agricultural low -carbon development (ALCD) holds substantial real -world significance for the lowcarbon sustainable growth of the global economy. Traditional urbanization, focusing on the "one-way" concentration of elements in cities and pursuing urban expansion's scale and speed, poses a threat to ALCD. In contrast, new -type urbanization (NTU), which emphasizes a "two-way" concentration of elements in both rural and urban areas, provides an approach to address this issue. Nevertheless, no research has investigated the relationship between NTU, particularly the new -type urbanization pilot policy (NTUPP), and ALCD. To fill this void, this research employs panel data from 210 Chinese cities from 2006 to 2019, using a difference -indifferences (DID) model to investigate the impact and mechanisms of NTUPP on ALCD for the first time. The study reveals: (1) NTUPP significantly boosts the level of ALCD, with cities that have implemented NTUPP showing an average enhancement of 19.13% in ALCD levels compared to those without NTUPP. (2) NTUPP chiefly fosters ALCD by improving agricultural technological efficiency rather than advancing technological progress. (3) Relative to smaller cities, northern cities, eastern cities, resource -based cities, major grainproducing areas, cities with substantial government intervention, and cities with lower levels of financial development, NTUPP is more efficacious in enhancing ALCD in larger cities, southern cities, central and western cities, non -resource -based cities, non -major grain -producing areas, cities with minimal government intervention, and cities with advanced financial development. (4) NTUPP advances ALCD in local cities and boosts ALCD in neighboring cities via spatial spillover effects. This research aids the Chinese government in clarifying the implementation effects of the new -type urbanization pilot policy based on its findings, offering theoretical underpinnings and practical evidence for government departments in shaping and assessing the policy, and providing insights and evidence for other nations worldwide to advance sustainable agricultural development via enhanced urbanization efforts.
摘要：
We explored the resource acquisition and growth strategies of plants adapting to different environments, focusing on the typical forest types of Sygera Mountain: Pinus armandii, Picea likiangensis var. Linzhiensis, Abies georgei var. Smithii, and Juniperus saltuaria. Then, we analyzed the nutrient content and stoichiometric ratios of C, N, P, and K in different plant organs (leaves, branches, trunks, and roots) to examine the stoichiometric characteristics and nutrient balance mechanisms in these forests. Results show that within the same forest type, different plant organs exhibit high C and low N, P, and K levels. N content in all organs followed the order leaves > branches > roots > trunks. During the growth phase, the concentrations of P and K in PLL and AGS follow the order branches > leaves > roots > trunks. In the dormant phase, the distribution in different organs had the order leaves > branches > roots > trunks. C content remained relatively stable over time. In the same organ across different forest types, increase in nitrogen content in plant leaves is an active adaptation of JS plants, indicating that JS has a conservative growth strategy and can adapt to environmental stress. Owing to the influence of seasons, the evolution process of N and P content fluctuates, allocating nutrients to supporting and transporting organs for resource optimization and allocation. The N and P content were lower in the growth phase than in the dormant phase. Seasonal variations in the C/N, C/P, and C/K ratios in different forests were inversely correlated with changes in N, P, and K content in plant organs, supporting the "growth rate hypothesis." Stoichiometric analysis suggests that different limiting elements exist in organs across various forest types. Principal component analysis indicates that the seasonal patterns of stoichiometric ratios in the organs of different forest types show species-specific characteristics, reflecting the evolutionary nutrient utilization strategies of plant genera. In summary, plant growth in different Sygera Mountain forest types is limited by N and P, with a high tendency toward nitrogen limitation. The nutrient utilization and distribution differences among various organs during different growth stages are primarily influenced by the limited availability of environmental nutrients and inherent physiological characteristics of the plants.
期刊：
Construction and Building Materials,2024年411:134273 ISSN：0950-0618
通讯作者：
Zheng, ZS;Huang, YJ
作者机构：
[Zeng, Chen] Wuhan Polytech Univ, Sch Civil Engn & Architecture, Wuhan 430023, Peoples R China.;[Liu, Gang; Wang, Xiang; Zheng, ZS; Zheng, Zhi-shan] Wuhan Municipal Engn Design & Res Inst Co Ltd, Wuhan 430023, Peoples R China.;[Zhang, Hui; Huang, Yu-jie] North Univ China, Sch Environm & Safety Engn, Taiyuan 030051, Peoples R China.
通讯机构：
[Zheng, ZS ] W;[Huang, YJ ] N;Wuhan Municipal Engn Design & Res Inst Co Ltd, Wuhan 430023, Peoples R China.;North Univ China, Sch Environm & Safety Engn, Taiyuan 030051, Peoples R China.
关键词：
Non-uniform corrosion of steel;3D mesoscale model;Mass transfer;Electrochemical corrosion;Bi-directional erosion
摘要：
Chloride-induced corrosion of steel in reinforced concrete (RC) exhibits complicated mechanisms such as chlo-ride diffusion, oxygen diffusion, electrochemical polarization, dissolution and deposition of steel. This work investigates the 3D non-uniform corrosion behavior of steel in RC by integrating all the possible mechanisms and mesoscale heterogeneities, for the first time. The anodic Tafel slope is used to describe the depassivation of steel induced by chloride, while the dynamic process of oxygen supply and consumption is considered as well as the inhibition of the cathodic reaction caused by oxygen concentration. The coupling of mass transfer and electro-chemical corrosion in RC is developed and validated. Mesoscale RC models with realistic aggregate, mortar, steel and interface are generated through a physically-based approach similar to casting procedures. The simulation results show that the 3D mesoscale RC model can simultaneously capture the non-uniform corrosion patterns of steel along the circumferential and longitudinal directions, offering significant application value in accurately predicting steel corrosion morphology. The random distribution of aggregates affects the local non-uniform corrosion of steel, but has little influence on the overall cumulative corrosion level. The local non-uniform corrosion of steel gradually weakens with the increase in chloride erosion time. The non-uniform corrosion of side steel is enhanced with the increase of the steel diameter and the decrease of the protective layer thickness. Bi-directional erosion mainly enhances the uniform corrosion of corner steel.
摘要：
Large scale of Cd-contaminated soil has become a serious threat to environmental safety and human health, whereas field applicability of existing remediation technologies has encountered numerous obstacles, such as poor stability, secondary pollution, long operation time, and high chemical cost. Herein, a novel activated calcite with significantly enhanced immobilization stability of Cd in soil has been synthesized based on mechanochemical technology. Our results demonstrate that the passivation rate of bioavailable Cd in Cd-contaminated soil (initial concentration 3 mg/kg) could be maintained at 65 % after 50 days of passivation cultured with activated calcite. Mechanistic studies have revealed that the outstanding performance is attributed to the enhancement of the CO32– and Ca2+ release properties of calcite through mechanical activation, leading to a 550 % increase in Ca2+ release. The CO32– fraction binds in situ to the free Cd in the soil, forming a stable carbonate-bound state. Simultaneously, the OH– released from the continuous hydrolysis of CO32– effectively regulates and stabilizes the soil pH within the neutral range. Further, SEM-EDS and sequential extraction procedures (SEP) analyses implied that the specific adsorption of Cd by in-situ formed iron oxides in soil was visibly related to the release of OH– from activated calcite and immobilized the free state Cd in the more stable iron oxide-bound state. The current work shows the high sustainability and stability of Cd-contaminated soil immobilization by activated calcite materials.
摘要：
The application of rapeseed protein in human foods is limited by residual antinutritive components and poor sensory quality. The effects of five extraction protocols on rapeseed protein yield, sensory, functional and nutritional properties were systematically evaluated in this study. In particular, the potential of weakly acidic salt (pH 6.5, 150mmol·L(-1) MgCl(2)) extraction as a mild method for recovering edible rapeseed protein was investigated compared with conventional alkali extraction. All salt-extracted proteins showed above 40% extraction yield and low antinutritional factor contents. They also had ideal amino acid patterns and better in vitro gastroduodenal digestibility than alkaline-extracted proteins. Additionally, the lighter color and odor, as well as better solubility, emulsion activity, foaming property, and water/oil holding capacity were found in weakly acidic salt extraction-ultrafiltered proteins. These findings suggest that weakly acidic salt extraction-ultrafiltration could be used for obtaining edible rapeseed protein, while extraction yield should be improved for scale application.
通讯机构：
[Liu, YJ; Liu, Y ] T;Tiangong Univ, Sch Chem, State Key Lab Separat Membranes & Membrane Proc, Tianjin 300387, Peoples R China.;Wuhan Univ, Coll Chem & Mol Sci, Wuhan 430072, Peoples R China.
关键词：
CRISPR/Cas9;Controlled-release;Immunotherapy;Metabolic therapy;Theranostic;Triple-negative breast cancer (TNBC)
摘要：
Metabolic heterogeneity and the tumor immunosuppressive microenvironment (TIME) of triple-negative breast cancer (TNBC) hinder therapeutic effectiveness. Although emerging metabolic therapy and immunotherapy show promise, they are limited by off-target effects and immune escape. Here, a redox-activatable, sequentially-releasing nanoparticle (AMANC@M) for tumor-targeted delivery of anticancer agents and CRISPR/Cas9 has been developed. AMANC@M can reverse the TIME through dual metabolic inhibition, thereby enhancing TNBC therapy. AMANC@M demonstrates excellent biosafety and targets tumors precisely through biomimetic hybrid membrane-mediated homologous homing and the enhanced permeability and retention (EPR) effect. Once internalized into tumor cells, the CRISPR/Cas9 system ("energy nanolock") is released through glutathione (GSH) cleavage and effectively knocks down the expression of lactate dehydrogenase A (LDHA) to suppress glycolysis. After peeling off of the gene editing shell, a newly synthesized targeted drug, CPI-Z2 ("nutrihijacker" and "energy nanolock"), is released in a controlled manner to block the mitochondrial tricarboxylic acid (TCA) cycle. Nitric oxide (NO) produced from loaded L-arginine enhances the efficiency of CPI-Z2 and reduces drug resistance. Combined with NO therapy, both blockades of nutrients and energy production transform the hypoxia and acidic TIME into an immunocompetent tumor microenvironment (TME) for tumor elimination. Furthermore, AMANC@M offers capabilities for photothermal (PT) therapy and provides clear imaging through PT, photoacoustic (PA), or computed tomography (CT) signals in tumor tissue. Thus, this study provides a new and promising sequentially stimuli-responsive targeting strategy for nanoparticle development, making it a potential treatment candidate for TNBC and other tumors.
摘要：
This study aimed to find a unique method to assess the textural properties of Niangao (glutinous rice cakes), to determine the relationship between the textural properties of rice cakes and the indicators of glutinous rice, and to identify the key indicators that significantly affect the textural properties of Niangao. The study encompassed the analysis of the chemical composition and pasting characteristics of 22 glutinous rice varieties, revealing the substantial impact of variety on lipid content, straight-chain starch content, and pasting performance. Subsequently, the textural features of the resulting Niangao were subjected to principal component analysis (PCA) to derive a mathematical method for evaluating their textural attributes, with the obtained scores employed in hierarchical cluster analysis (HCA) to identify 12 key textural characteristics. Further analysis using stepwise linear regression (SLR) demonstrated that the regression model incorporating final and peak viscosities of the glutinous rice significantly predicted the composite score of the Niangao's textural properties. This highlights the importance of final and peak viscosities as key indicators for assessing the textural quality of Niangao.
通讯机构：
[Mu, D ; Wang, C ] B;Beijing Jiaotong Univ, Sch Econ & Management, Beijing, Peoples R China.;Beijing Univ Technol, Coll Econ & Management, Beijing, Peoples R China.
关键词：
EV LIBs;Geopolitical disruptions;Supply chain network;Vulnerability assessment;Disruption ripple effect
摘要：
In the rapidly expanding global electric vehicle lithium -ion battery supply chain network (EV LIB SCN), intricate intercontinental and interrelated connections render it susceptible to geopolitical disturbances. The complex supplier-buyer dynamics within this network facilitate the propagation of disruptions, complicating the identification and evaluation of the consequences of geopolitical disruptions. Existing recommendations, grounded in macro -level material flow data, offer limited guidance for EV LIB -associated enterprises in managing such disruptions. To address this gap, this study constructs a meso-level EV LIB SCN incorporating realistic supplier-buyer relationship data among relevant firms. A supply chain network vulnerability (SCNV) index has been designed to quantify the disruption impact. Furthermore, a geopolitical disruption diffusion (GDD) model has been developed to simulate the disruption propagation in two distinct scenarios, i.e., single -region blockade and inter -region blockade. This study broadens the scope of EV LIB SCN investigations from macro- to meso-level analyses, and the evaluation of both direct and indirect impacts of geopolitical disruptions enhances comprehension of geopolitical disruption risks in global supply chain networks.
