摘要:
Polyethyleneimine (PEI) is a non-viral gene vector of frequent concern because of its high transfection efficiency. However, its clinical application is limited by the cytotoxicity caused by high positive charge density. Therefore, reducing toxicity and maintaining high transfection efficiency through appropriate strategies are of great significance to promote the development of PEI as gene vector. In order to reduce the toxicity of PEI, this work studied the feasibility of encapsulating PEI/DNA complexes by using the coordination between tannic acid (TA) and metal ions (Mn+). Firstly, TA formed a coating layer on the surface of PEI/DNA complex through electrostatic action, and then the quaternary complex (Mn+(TA/PEI/DNA)) system was prepared by the coordination between metal ions and TA. In order to better construct a gene delivery system with high transfection efficiency and low toxicity, the effects of PEI molecular weight, mass ratio of PEI/DNA, mass ratio of TA, and types of metal ions on the transfection efficiency, cytotoxicity, particle size, zeta potential, and cell uptake pathway of the quaternary complex were studied. The results showed that the quaternary complex based on PEI70k and encapsulated by coordination between TA and potassium ions showed better safety than PEI70k/DNA while maintaining high transfection efficiency. This showed that the strategy of coordination encapsulation of TA and ions to improve the safety of PEI as gene carrier is feasible, which provides a new reference for the design of gene delivery system based on PEI.
关键词:
chemical pesticides;biopesticides;rice–crayfish integrated system;combination use
摘要:
Biopesticides are perceived as a feasible alternative to chemical pesticides, providing an effective approach to pest management while mitigating the undesirable effects of chemical pesticide overuse. Yet, due to the distinctive attributes of the two types of pesticides, farmers often adopt a combination of both. This study aimed to probe the interdependent relationship underlying the mixed use of chemical pesticides and biopesticides by farmers in the rice–crayfish integrated system (RCIS) in Hubei province, China. We also sought to identify influencing factors and associated consequences of this practice. Given that the adoption of chemical pesticides and biopesticides by farmers is not mutually exclusive, a multivariable probit model was utilized to estimate simultaneous pesticide applications. Survey data from 736 RCIS farmers revealed that 10.50% of the sample farmers employ a combination of chemical pesticides and biopesticides. A substitution relationship was observed between the adoption of chemical pesticides and biopesticides by farmers, whereas no significant complementary relationship was found in the application of the two types of pesticides. The primary motivation for the mixed use of pesticides by farmers is to achieve superior pest control efficacy. Notably, when integrating the use of pesticides, farmers pay less attention to factors such as resource endowments and multiple production objectives. Further analysis indicated that farmers display significant concern for pesticide attributes including insecticidal efficacy, toxicity, insecticidal spectrum, and validity period. The mixed use of pesticides has resulted in a decreased frequency of pesticide application but has escalated the cost. While the mixed use of pesticides elevated the selling price of rice, no significant improvement was observed in yield and output value. The insights garnered from this study offer strategic implications for policymakers on how to enhance the judicious adoption of pesticides by farmers.
摘要:
Lotus (Nelumbo nucifera Gaertn.), an important aquatic plant in horticulture and ecosystems, has been cultivated for more than 7000 years and domesticated into three different subgroups: flower lotus, rhizome lotus, and seed lotus. To explore the domesticated regions of each subgroup, re-sequencing data of 371 lotus accessions collected from the public database were aligned to the genome of 'China-Antique (CA)'. Unmapped reads were used to build the dispensable genome of each subgroup using a metagenome-like assembly strategy. More than 27 Mb of the dispensable genome in these three subgroups and the wild group was assembled, of which 11,761 genes were annotated. Some of the contigs in the dispensable genome were similar to the genomic segments of other lotus accessions other than 'CA'. The annotated genes in each subgroup played essential roles in specific developmental processes. Dissection of selective signals in three cultivated subgroups also demonstrated that subgroup-specific metabolic pathways, such as the brassinosteroids metabolism enrichment in FL, associated with these selected genes in each subgroup and the contigs in dispensable genome nearly located in the domesticated regions of each subgroup, respectively. Our data presented a valuable resource for facilitating lotus genomic studies, complemented the helpful information to the reference genome, and shed light on the selective signals of domesticated subgroups.
作者机构:
[Cheng, Shuiyuan; Rao, Shen; Cong, Xin; Rao, S; Liu, Haodong; Liu, Xiaomeng; Cheng, Hua] Wuhan Polytech Univ, Natl R&D Ctr Se Rich Agr Prod Proc Technol, Sch Modern Ind Selenium Sci & Engn, Wuhan 430048, Peoples R China.;[Cong, Xin; Gong, Jue] Enshi Se Run Mat Engn Technol Co Ltd, Enshi 445000, Peoples R China.
通讯机构:
[Cong, X ; Rao, S] W;Wuhan Polytech Univ, Natl R&D Ctr Se Rich Agr Prod Proc Technol, Sch Modern Ind Selenium Sci & Engn, Wuhan 430048, Peoples R China.;Enshi Se Run Mat Engn Technol Co Ltd, Enshi 445000, Peoples R China.
摘要:
Cardamine violifolia, a species belonging to the Brassicaceae family, is a novel vegetable crop that is rich in glucosinolates. However, the specific glucosinolate profiles in this species remain unknown. In the present study, four parts of C. violifolia were collected including central leaves (CLs), outer leaves (OLs), petiole (P), and root (R). The highest level of total glucosinolate was observed in the R. A total of 19 glucosinolates were found in C. violifolia. The predominant glucosinolate compounds were 3-methylbutyl glucosinolate, 6-methylsulfinylhexyl glucosinolate, Indol-3-ylmethyl glucosinolate, 4-methoxyglucobrassicin, and neoglucobrassicin. A transcriptome analysis showed that 16 genes, including BCAT1, BCAT3-6, CYP79A2, CYP79B2-3, CYP83A1, CYP83B1, and SOT17-18, and nine metabolites, such as valine, tryptophan, and 1-methylpropyl glucosinolate, were enriched in the glucosinolate biosynthesis pathway. These genes may be involved in the regulation of glucosinolate accumulation among the four parts. A weighted gene co-expression analysis showed that five genes were predicted to regulate glucosinolate accumulation, including ABC transporter G family member 19, 3-ketoacyl-CoA synthase 19, and pyruvate decarboxylase 1. This study deepens our understanding of the nutrient quality of C. violifolia and provides insights into the regulatory mechanism of glucosinolate accumulation in C. violifolia.
通讯机构:
[Liu, Gang] C;College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, People's Republic of China. Electronic address:
关键词:
Whey protein isolate;Short-chain inulin;Glycosylation;Cyanidin-3-glucoside;Pickering emulsion;Curcumin;Physicochemical stability
摘要:
Protein-polysaccharide-polyphenol delivery systems function as a promising tool to deliver bioactive ingredients aiming to improve their solubility and bioavailability. In this study, whey protein isolate (WPI), short-chain inulin (SCI), and cyanidin-3-glucoside (C3G) were first used to stabilize Pickering emulsions. The physicochemical properties and stability of curcumin encapsulated or not in Pickering emulsions were explored. Results showed that glycosylation and C3G reduced surface and interfacial tension on protein surfaces and inhibited the aggregation of emulsion droplets, thereby reducing the emulsion's particle size. WPI-SCI/C3G stabilized Pickering emulsion had the best stability. The CLSM results showed that the WPI-SCI and WPI-SCI/C3G stabilized emulsions were uniformly dispersed, suggesting that glycosylation and the interaction between protein and C3G enhanced the adsorption capacity of the interfacial protein and improved the stability of the Pickering emulsion. The retention rates of curcumin-loaded WPI-SCI- (67.34%) and WPI-SCI/C3G- (77.07%) stabilized Pickering emulsions on day 8 of storage were higher than those in WPI- (33.97%) and WPI/C3G- (37.02%) stabilized emulsions, and the degradation half-life was also extended from 7days to >15days. These findings provide a theoretical basis for the application of WPI Pickering emulsion and indicate a useful means for the delivery of bioactive components.
摘要:
Inulin exhibits the abilities to increase the survival rate of yeast in frozen dough during the storage time and improve the quality of the bread prepared therefrom. Summary Effects of inulin on yeast in frozen dough and bread prepared from the frozen dough were examined in this study. The number of hydrogen bonds increased accordingly and both short‐chain and long‐chain inulin in the dough could combine with more water and reduce water migration. And both these inulin types improved the survival rate and fermentability of yeast. Inulin addition to dough frozen for 4 weeks increased yeast survival by 32–36.6% compared with doughs lacking inulin. The fermentation ability in doughs stored with short‐chain inulin was greater than those stored with long‐chain inulin although these compounds significantly increased the specific volume, improved the slice structure and reduced bread hardness. The quality improvement using short‐chain inulin was significantly better than that of long‐chain inulin. Therefore, our results demonstrated that the addition of inulin could effectively reduce the damage to yeast cells and improve the quality of frozen dough and bread.
作者:
Fang, Chao;Chen, Xiqu;Zhang, Juanjuan;Xia, Xiaodong;Weng, George J.
期刊:
Journal of Physics D: Applied Physics,2023年56(4):045301 ISSN:0022-3727
通讯作者:
George J Weng
作者机构:
[Fang, Chao; Chen, Xiqu] Wuhan Polytech Univ, Dept Elect & Elect Engn, Wuhan 430023, Hubei, Peoples R China.;[Zhang, Juanjuan] Lanzhou Univ, Key Lab Mech Environm & Disaster Western China, Minist Educ China, Lanzhou 730000, Gansu, Peoples R China.;[Zhang, Juanjuan] Lanzhou Univ, Coll Civil Engn & Mech, Dept Mech & Engn Sci, Lanzhou 730000, Gansu, Peoples R China.;[Xia, Xiaodong] Cent South Univ, Sch Civil Engn, Changsha 410083, Peoples R China.;[Weng, George J.] Rutgers State Univ, Dept Mech & Aerosp Engn, New Brunswick, NJ 08903 USA.
通讯机构:
[George J Weng] D;Department of Mechanical and Aerospace Engineering, Rutgers University, New Brunswick, NJ 08903, United States of America
关键词:
graphene films;EMI shielding;electromagnetic plane wave;path;wrinkles;Monte Carlo
摘要:
In this paper, the electromagnetic interference shielding effectiveness (EMI SE) of multilayer graphene films is studied by the Monte Carlo (MC) method. First, we use the transfer matrix method to study the transmission and reflection of electric fields of the incident light passing through the graphene plane and the dielectric interface, and then determine the reflection and transmission probability of the light. In this way, the path of light can be obtained by the MC method. From the distance and emission direction of a large number of lights passing through the film, the expected value of the emitted light field is calculated and the EMI SE is determined. The obtained results of SE are shown to be in good agreement with several sets of experimental data. This model also takes into account the influence of wrinkles of graphene. It is found that EMI SE first increases and then decreases with the increase of wrinkle. The effects of film thickness, conductivity, and dielectric constant on EMI SE in the X-band frequency range are also investigated.
摘要:
Abstract: Foliar application of selenium (Se) fertilizer has been widely used in the production of Se-rich rice. However, the effect of Se-nanoparticles (SeNPs) fertilizer on rice quality remains largely unknown. Two bioSeNPs fertilizers were applied at different Se concentrations to explore the effect of the foliar application of SeNPs fertilizer on various rice grain qualities. The results showed that Se treatments resulted in 1.93–9.09 and 1.89–7.73 folds of total Se contents in brown and polished rice relative to the CK, respectively. Moreover, the Se treatments led to 1.04–2.33 folds increases in Cd contents, 14.6–26.4% decreases in As contents, a 13.9–16.7% reduction in Cr contents and no changes in Pb contents to that of the CK in rice grains. In addition, SeNPs exhibited no obvious impact on rice eating and cooking quality, and only the XY1 treatment could slightly improve the fatty acid content. Interestingly, Se treatments enhanced the contents of more than half of volatile organic compounds in brown rice. In general, SeNPs fertilizer XY at 6.4 g/ha was identified as the optimum choice for improvement in grain Se contents and grain qualities. Keywords: rice; selenium; grain qualities; volatile organic compounds
通讯机构:
[Feng-Lei Jiang] S;Sauvage Center for Molecular Sciences, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, P. R. China
通讯机构:
[Hongsen Xu] H;Hubei Key Laboratory of Animal Nutrition and Feed Science, School of Animal Science and Nutritional Engineering, Wuhan Polytechnic University, Wuhan 430023, China
摘要:
This study investigated the effects of dietary chitosan on growth, antioxidant, immunity, intestinal morphology and resistance against Aeromonas hydrophila of hybrid sturgeon (Acipenser baerii female x Acipenser schrenckii male). Sturgeons (18.18 +/- 0.08 g) were randomly divided into four groups, fed with chitosan-supplemented diets for 8 weeks and then infected with A. hydrophila. The results showed significant differences of body weight gain, specific growth rate and feed conversion ratio in sturgeon fed chitosan and control diets. The oral administration of chitosan significantly increased the acid phosphatase, alkaline phosphatase, lysozyme, myeloperoxidase, su-peroxide dismutase, glutathione peroxidase and catalase activities, as well as the complement 3 and 4 contents and disease resistance against A. hydrophila. Moreover, enhancement of muscular thickness and goblet cells in mid intestine and increase of muscular thickness and villus height in spiral valve were observed in the chitosan supplemented groups. In addition, dietary chitosan-supplemented diets mitigated the changes of antioxidant and immune activity induced by A. hydrophila challenge, as well as prevented fish from bacterial invasion. The optimal dose was 3.00 g chitosan/kg diet for hybrid sturgeon.
摘要:
With the convergence of China's "entrepreneurial society" and the "digital age," part-time entrepreneurial behavior (PEB) has received widespread attention as a new form of labor relations and entrepreneurial paths. Entrepreneurial self-efficacy (ESE) is an important indicator of entrepreneurs' confidence in their skills and in coping with uncertain entrepreneurial challenges. However, studies on how ESE affects different types of PEB have not been thoroughly carried out. To fill the existing research gap, the primary objective of this paper is to thoroughly investigate the individual impact of three key factors, namely organizational management self-efficacy (OMSE), opportunity development self-efficacy (ODSE), and risk-taking self-efficacy (RTSE), on economic profit-driven part-time entrepreneurial behavior (EPEB) and self-value-driven part-time entrepreneurial behavior (SPEB), respectively. Based on the "cognition-environment-behavior" logic of triadic reciprocal determinism, this study also aims to examine the moderating effect of entrepreneurial resources (ERs) and attempt to understand how external factors regulate internal factors (OMSE, ODSE, and RTSE) to form the results of EPEB and SPEB. This study adopted a quantitative research approach with a questionnaire survey, taking 457 part-time entrepreneurs in 11 major cities as subjects. The main results show that (1) OMSE and ODSE have a positive effect on EPEB, while RTSE has an inverted U-shaped effect on it; (2) ODSE and RTSE have a positive effect on SPEB, and OMSE has an inverted U-shaped effect on it; and (3) ERs play a positive moderating role in the above processes. The results can not only effectively guide part-time entrepreneurs in PEB but also provide a reference for the government to improve part-time entrepreneurship policies.
摘要:
Photothermal therapy (PTT) is considered to be one of the promising methods to combat pathogenic bacteria. However, traditional PTT is prone to generate undesired temperature increase to surrounding normal tissues, which limits the application of PTT. Herein, an acid-responsive PTT system (Au nanoparticles system: AuNPs-S) was constructed based on the photothermal feature of spherical gold nanoparticles (AuNPs) and the low pH of the bacterial infected site. AuNPs-S is composed of two kinds of AuNPs: AuNPs modified with Asp-Asp-Asp-Asp-Asp-Cys (peptide A) were denoted as AuNPs-A; AuNPs modified with 2,3-dimethylmaleic anhydride (DA) grafted Lys-Gly-Gly-Lys-Gly-Gly-Lys-Cys (peptide B) were denoted as AuNPs-B/DA. AuNPs-B/DA with an acid-responsive moiety showed a charge-convertible feature. The negatively charged AuNPs-B/DA became positively charged AuNPs-B at low pH, aggregating with the negatively charged AuNPs-A via an electrostatic interaction, reaching the threshold to the interparticle plasmonic coupling effect among AuNPs, thereby killing bacteria precisely under the irradiation of near-infrared (NIR) light through the elevated temperature at the targeted area. This acid-responsive PTT strategy supplies an excellent mode for combating bacterial infections with no vital damage to normal tissues.
期刊:
IEEE Transactions on Knowledge and Data Engineering,2023年35(1):264-275 ISSN:1041-4347
通讯作者:
Yuan, C.
作者机构:
[Liao, Tingting] Chinese Acad Sci, Inst Automat, Ctr Biometr & Secur Res CBSR, Natl Lab Pattern Recognit NLPR,CASIA, Beijing 100190, Peoples R China.;[Liao, Tingting] Univ Chinese Acad Sci UCAS, Sch Artificial Intelligence, Beijing 100049, Peoples R China.;[Lei, Zhen] Chinese Acad Sci, Hong Kong Inst Sci & Innovat, Ctr Artificial Intelligence & Robot, Hongkong 999077, Peoples R China.;[Zhu, Tianqing] Univ Technol Sydney, Fac Engn & Informat Technol, Ultimo, NSW 2007, Australia.;[Li, Yaqin; Yuan, Cao; Zeng, Shan] Wuhan Polytech Univ, Coll Math, Comp Sci Dept, Wuhan 430023, Peoples R China.
通讯机构:
[Yuan, C.] W;Wuhan Polytechnic University, China
关键词:
K nearest neighbor;distance metric learning;prototype reduction
摘要:
K Nearest Neighbor (kNN) has gained popularity in machine learning due to its simplicity and good performance. However, kNN faces two problems with classification tasks. The first is that an appropriate distance measurement is required to compute distances between test sample and training samples. The other is the highly computational complexity due to the requirement of searching the nearest neighbors in the whole training data. In order to mitigate these two problems, we propose a novel method named KCNN to enhance the performance of kNN. KCNN uses convolutional neural networks (CNN) to learn a suitable distance metric as well as prototype reduction to learn a reduced set of prototypes which can represent the original set. It has several superiorities compared with related methods. The combination of CNN and kNN empowers it to extract discriminative hierarchical features with which kNN can easily classify. KCNN learns spatial information on an image instead of considering it as a vector to learn distance metric. Moreover, KCNN simultaneously learns a reduced set of prototypes, which help improve classification efficiency and avoid noisy samples of the massive training set. The proposed method has a better robustness and convergence than CNN, especially when projecting input data into a rather low-dimension space.
摘要:
Abstract: Genetically modified (GM) technology is of great significance for increasing crop production, protecting biodiversity, and reducing environmental pollution. However, with the frequent occurrence of safety events regarding GM foods, more and more disputes have arisen over the potential safety of transgenic technology. It is particularly necessary to find a fast and accurate method for transgenic product identification. In this research, mid-infrared spectroscopy, coupled with chemometric methods, was applied to discriminate GM maize from its non-GM parent. A total of 120 GM maize and 120 non-GM maize samples were prepared, and the spectral information in the range of 400–4000 cm−1 was collected. After acquiring the spectra, wavelet transform (WT) was used to preprocess the data, and k-means was carried out to split all samples into calibration and prediction sets in the ratio of 2:1. Principal component analysis (PCA) was then conducted to qualitatively distinguish the two types of samples, and an apparent cluster was observed. Since the full spectrum covered a large amount of data and redundant information, we adopted the successive projections algorithm (SPA) to select optimal wavelengths for further analysis. Chemometrics, including partial least squares-discriminant analysis (PLS-DA), the k-nearest neighbor algorithm (KNN), and the extreme learning machine (ELM), were performed to establish classification models based on full spectra and optimal wavelengths. The overall results indicated that ELM models based on full spectra and optimal spectra showed better accuracy and reliability, with a 100% recognition rate in the calibration set and a 98.75% recognition rate in the prediction set. It has been confirmed that mid-infrared spectroscopy, combined with chemometric methods, can be a novel approach to identify transgenic maize. Keywords: genetically modified maize; identification; mid-infrared spectroscopy; chemometric methods
期刊:
International Journal of Geomechanics,2023年23(3):06023002 ISSN:1532-3641
通讯作者:
Juehao Huang
作者机构:
[Huang, Juehao] Chinese Acad Sci, Inst Rock & Soil Mech, State Key Lab Geomech & Geotech Engn, Wuhan 430071, Peoples R China.;[Huang, Juehao; Yan, Xiaoling; Chen, Jian] Univ Chinese Acad Sci, Sch Engn Sci, Beijing 100049, Peoples R China.;[Chen, Jian; Fu, Xiaodong] Inst Rock & Soil Mech, Chinese Acad Sci, State Key Lab Geomech & Geotech Engn, Wuhan 430071, Peoples R China.;[Chen, Jian] Inst Rock & Soil Mech, Chinese Acad Sci, Hubei Key Lab Geoenvironm Engn, Wuhan 430071, Peoples R China.;[Chen, Jian] Ningbo Univ Technol, Soft Soil Res Ctr, State Key Lab Geomech & Geotech Engn, Ningbo 315211, Peoples R China.
通讯机构:
[Juehao Huang] A;Assistant Professor, State Key Laboratory of Geomechanics and Geotechnical Engineering, Institute of Rock and Soil Mechanics, Chinese Academy of Sciences, Wuhan 430071, China;Assistant Professor, School of Engineering Science, Univ. of Chinese Academy of Sciences, Beijing 100049, China
摘要:
The mechanical behaviors of subgrade soils will change after cyclic loading, resulting in a modification of foundation bearing capacity and the development of additional settlement. Recognizing this, cyclic triaxial tests were conducted to remolded laterite clay, and reconsolidation was allowed. During the process, the cyclic loading was applied under undrained and partially drained conditions, respectively. Results show that the variations of both postcyclic undrained strength and elastic modulus versus degree of reconsolidation are different: the postcyclic elastic modulus increases with an increase of the degree of reconsolidation, while the undrained strength decreases a little under the condition of the large degree of reconsolidation. Furthermore, there are specific degrees of reconsolidation, making the postcyclic undrained strength and elastic modulus equal to the corresponding test results of the specimen without reconsolidation. Moreover, the greater the number of cycles, the larger the elastic modulus of the specimens with full reconsolidation, and the lower the elastic modulus of the specimens without reconsolidation. The undrained strengths of the specimens with full reconsolidation are all higher than those of the specimens without cyclic loading, whereas those of the specimens without reconsolidation are all lower. In addition, both postcyclic undrained strength and elastic modulus between cyclic loading under undrained and partially drained conditions are compared: the elastic moduli of the specimens under partially drained conditions are greater than those obtained under undrained conditions, while the undrained strengths obtained under partially drained conditions are equal to that under undrained conditions at a certain number of cycles.
关键词:
Bacillus cereus;Flow cytometry;Propidium monoazide;Fluorescence in situ hybridization
摘要:
Fluorescence-labeled antibodies and viability indicators are routinely employed in conjunction with flow cytometry (FCM) to rapidly quantify viable target bacteria, ensuring food safety. However, the specific detection of Bacillus cereus, a prominent bacterium causing food poisoning, proves challenging because of its close phylogenetic relationship with other Bacillus species. Moreover, the accuracy of viability indicators in identifying viable B. cereus has been underexplored. This study established a rapid and specific method for quantifying viable B. cereus. The membrane-permeable dye propidium monoazide was identified as an effective means to differ-entiate between viable and non-viable B. cereus cells. Subsequently, fluorescence in situ hybridization was introduced to specifically identify viable B. cereus cells. Furthermore, FCM was utilized for the rapid and accurate quantification of labeled viable B. cereus cells. Following optimization, the method demonstrated notable specificity and robust anti-interference ability, with B. cereus recovery rates in milk powder and chicken breast after pretreatment ranging from 96.3% to 97.7%. Viable B. cereus cells were accurately quantified within 1.5 h, with a linear range of 102 to 107 cells/g. In conclusion, this developed method enables the rapid and specific quantification of viable B. cereus, offering a novel approach to the detection of specific foodborne pathogens.
作者机构:
[Yu, Denghang; Cai, Fangfang; Li, Shuheng] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Safe, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Zhang, Hang] Hubei Water Resources & Hydropower Sci & Technol i, Hubei Water Resources Res Inst, Wuhan 430070, Peoples R China.;[Li, Renhui] Wenzhou Univ, Sch Life & Environm Sci, Wenzhou 325035, Peoples R China.
通讯机构:
[Fangfang Cai] H;Hubei Key Laboratory of Animal Nutrition and Feed Science, Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Wuhan Polytechnic University, Wuhan 430023, China
摘要:
Abstract: Cartusia hunanesis sp. nov. was isolated from a stream in China, and two strains (ZJJ02 and ZJJ03) of which were inquired using morphological features, ecological evidence, and molecular data consisting of the 16S rRNA gene and 16S–23S rRNA intergenic transcribed spacer (ITS) region. Cartusia hunanesis varies from the type species Cartusia fontana by having only a single trichome in the sheath and large granules near the cross wall. The investigated strains of C. hunanesis were revealed to be a sister clade of C. fontana, according to the phylogenetic analysis based on the 16S rRNA gene. In addition, the Cartusia was clustered with the family Oculatellaceae members, genera Pegethrix and Elainella. These two strains displayed 97.6% similarity to C. fontana. The ITS region of C. hunanesis was found to be considerably distinct from that of C. fontana in terms of the secondary structure, which demonstrated that C. hunanesis is a novel species owing to the divergences in its morphological and genetic data compared with the related C. fontana. Keywords: cyanobacteria taxonomy; polyphasic approach; Cartusia; new species; 16S rRNA gene; 16S–23S ITS