作者机构：
[Liao, Yangwei; Zhang, Wei; Mu, Yaoqin; Zhang, Lei; Xiao, Xianjin; Hu, Hao; Li, Longjie; Yan, Bei; Ye, Zhengxin] Huazhong Univ Sci & Technol, Inst Reprod Hlth, Tongji Med Coll, Wuhan 430030, People R China.;[Wang, Hongbo; Dong, Kejun; Zhao, Rong; Zhang, Wei; Shu, Wan] Huazhong Univ Sci & Technol, Union Hosp, Tongji Med Coll, Dept Obstet & Gynecol, Wuhan 430000, People R China.;[Lu, Yaping] Sinopharm Genom Technol Co Ltd, Wuhan 430000, People R China.;[Wan, Chong] Tsinghua Univ, Precis Med Ctr, Yangtze Delta Reg Inst, Jiaxing 314006, Peoples R China.;[Sun, Qiangqiang] Life Hlth Care Clin Labs, Beijing 100000, Peoples R China.
通讯机构：
[Longjie Li; Xianjin Xiao] I;[Hongbo Wang] D;Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology , Wuhan 430030, China<&wdkj&>School of Life Science and Technology, Wuhan Polytechnic University , Wuhan 430023, China<&wdkj&>Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology , Wuhan 430000, China<&wdkj&>Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology , Wuhan 430030, China
摘要：
Although CRISPR-Cas12a [clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 12a] combiningpre-amplification technology has the advantage of high sensitivity in biosensing, its generality and specificity are insufficient, which greatly restrains its application range. Here, we discovered a new targeting substrate for LbaCas12a (Lachnospiraceae bacterium Cas12a), namely double-stranded DNA (dsDNA) with a sticky-end region (PAM-SE+ dsDNA). We discovered that CRISPR-Cas12a hadspecial enzymatic properties for this substrate DNA, including the ability to recognize and cleave it without needing a protospacer adjacent motif (PAM) sequence and a high sensitivity to single-base mismatches in that substrate. Further mechanism studies revealed that guide RNA (gRNA) formed a triple-stranded flap structure with the substrate dsDNA. We also discovered the property of low-temperature activation of CRISPR-Cas12a and, by coupling with the unique DNA hybridization kinetics at low temperature, we constructed a complete workflow for low-abundance point mutation detection in real samples, which was fast, convenient and free of single-stranded DNA (ssDNA) transformation. The detection limits were 0.005-0.01% for synthesized strands and 0.01-0.05% for plasmid genomic DNA, and the mutation abundances provided by our system for 28 clinical samples were in accordance with next-generation sequencing results. We believe that our work not only reveals novel information about the target recognition mechanism of the CRISPR-Cas12a system, but also greatly broadens its application scenarios.
摘要：
Purpose
The purpose of this paper is to develop a high-performance composite emulsion cement waterproof coating. The coating has excellent durability and is effective in protecting cement mortar substrates from harmful ions.
Design/methodology/approach
The polymer cement waterproof coatings with different emulsion compounding ratios were tested for mechanical properties and water resistance after alkali immersion, water immersion, thermal aging and UV aging, and the coatings were analyzed by infrared spectroscopy after aging to evaluate its durability. Meanwhile, the coating that presents favorable durability was applied to cement mortar test blocks. The protective effect of the coating on the test blocks was tested by immersion method, and X-ray diffraction analysis was performed on the eroded test blocks.
Findings
The coating with neoprene latex/acrylate latex weight ratio of 90/10 presents favorable durability and has superior overall performance. Besides, when it is applied to cement mortar blocks, the coatings effectively reduced the erosive effect of harmful ions on cement mortar blocks, resulting in much lower mass change ratios and less internal structural damage of the blocks significantly.
Originality/value
The obtained coating will be of great application potential for use in building waterproofing construction. Moreover, the coating can practically prevent chloride ions and sulfate ions from penetrating cement-based materials.
期刊：
International Journal of Clinical Practice,2022年2022 ISSN：1368-5031
通讯作者：
Zhang, H.
作者机构：
[Zhang, Hongping; Zhao, Yinshaung] Wuhan Polytech Univ, Coll Med & Hlth Sci, Wuhan 430023, Peoples R China.;[Han, Mo; Wei, Feng] Ctr Dis Prevent & Control Huangpi Dist Wuhan, Wuhan 430300, Peoples R China.;[Chen, Jianquan] Dept Dis Control Hlth & Family Planning Commiss H, Wuhan 430300, Peoples R China.;[Peng, Songxu] Cent South Univ, Xiangya Sch Publ Hlth, Xiangya Sch Med, Dept Maternal & Child Hlth, Changsha 410008, Peoples R China.;[Du, Yukai] Huazhong Univ Sci & Technol, Tongji Med Coll, Sch Publ Hlth, Dept Social Med & Hlth Management, Wuhan 430030, Peoples R China.
通讯机构：
College of Medicine and Health Science, Wuhan Polytechnic University, Wuhan, China
摘要：
<i>Aim</i>. The aim of the study was to provide evidence for the prevention and reduction of falls in the elderly living in rural areas by analyzing epidemiological data of falls among the rural older people (>65 years old) and identifying the risk and protective factors. <i>Methods</i>. This study analyzed the sociodemographic characteristics, living environment, lifestyle, chronic disease condition, mental health, activities of daily living (ADL), and detailed information of falls of 3752 rural elderly. Rank tests, chi-square tests, and binary logistic regression were used for data analysis. <i>Results</i>. The prevalence of falls was 30.0%, and the 75–84-years age group had the highest fall rate (18.8%). According to the binary logistic regression analysis, six variables, including roughage intake frequency, age, gender, cane use, floor tiles, and IADL, were involved in the fall patterns. Low roughage intake (OR = 2.48, 95% CI 1.24–4.97), female gender (OR = 2.12, 95% CI 1.48–3.05), the use of a cane (OR = 2.11, 95% CI 1.08–4.10), and medium IADL (OR = 2.02, 95% CI 1.89–2.32) were the top four risk factors. <i>Conclusion</i>. The fall in the rural elderly was mainly due to the poor living and working conditions. Routine fall assessment could address several preventable risk factors to reduce the prevalence and mitigate the harm of falls.
通讯机构：
[Yan Liu] C;College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, P. R. China<&wdkj&>Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education, College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan Polytechnic University, Wuhan, P.R. China<&wdkj&>Hubei Key Laboratory for Processing and Transformation of Agricultural Products (Wuhan Polytechnic University), College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, P.R. China
摘要：
MEMS capacitive accelerometer for the Internet of Things (IoT) applications is designed with open-loop structure rather than closed-loop structure to achieve low power consumption. In the open-loop structure, voltage control readout technique is preferred for low cost. However, the voltage control readout technique suffers from low dynamic range and low power efficiency (in terms of $\mathbf {FoM}$ ). In this paper, the voltage control ratiometric (VCR) readout technique is proposed to improve both dynamic range and power efficiency. The VCR readout technique is demonstrated in a readout circuit fabricated in a commercial 0.18 $\mu {\mathrm{ m}}$ 1.8V/5.0V CMOS process. Compared to the traditional voltage readout circuit fabricated with the same CMOS process and tested with the same sensing element, the VCR readout circuit improves full input signal range by $\mathbf {3.5dB}$ (from $\boldsymbol {\pm 8g}$ to $\boldsymbol {\pm 12g}$ ) and the noise floor by $\mathbf {9.5dB}$ (from $\mathrm {\mathbf {804~\mu g/}}\sqrt {\mathbf {Hz}} $ to $\mathbf {270~\mu g/}\sqrt {\mathbf {Hz}} $ ). As a result, the dynamic range is improved by $\mathbf {13.0dB}$ (from $\mathbf {44.0dB}$ to $\mathbf {57.0dB}$ ), the $\mathbf {Fo}\mathbf {M}_{\mathbf {1}}$ is improved from $\mathbf {310pJ}$ to $\mathrm {\mathbf {83pJ }}$ and the $\mathbf {Fo}\mathbf {M}_{\mathbf {2}}$ is improved from $\mathbf {1977~\mu W\cdot \mu g/Hz}$ to $\mathbf {796~\mu W\cdot \mu g/Hz}$ .
作者机构：
[Wang, Qian; Zhao, Lin; Wang, Mali; Zhang, Qipeng] Liaocheng Univ, Sch Geog & Environm, Dept Geog Informat Sci, Liaocheng 252059, Shandong, Peoples R China.;[Wu, Jinjia] Liaocheng Univ, Grand Canal Res Ctr, Grand Canal Res Inst, Liaocheng 252000, Shandong, Peoples R China.;[Zhou, Wei] Southwest Univ, Sch Geog Sci, Chongqing Jinfo Mt Karst Ecosyst Natl Observat &, Chongqing 400715, Peoples R China.;[Deng, Meie] Wuhan Polytech Univ, Sch Management, Wuhan 430048, Peoples R China.
通讯机构：
[Qipeng Zhang] D;Department of Geographic Information Science, School of Geography and Environment, Liaocheng University, Liaocheng 252059, China<&wdkj&>Author to whom correspondence should be addressed.
关键词：
random forest;grassland drought monitoring;SPEI;drought
摘要：
The accuracy of drought monitoring models is crucial for drought monitoring and early warning. Random forest (RF) is being used widely in the field of artificial intelligence. Nonetheless, the application of a random forest model in grassland drought monitoring research is yet to be further explored. In this study, various drought hazard factors were integrated based on remote sensing data, including from the Moderate Resolution Imaging Spectroradiometer (MODIS) and Global Precipitation Measurement (GPM), as multisource remote sensing data. Based on the RF, a comprehensive grassland drought monitoring model was constructed and tested in Inner Mongolia, China, as an example. The critical issue addressed is the construction of a grassland drought disaster monitoring model based on meteorological data and multisource remote sensing data by using an RF model, and the verification of the accuracy and reliability of its monitoring results. The results show that the grassland drought monitoring model could quantitatively monitor the drought situation in Inner Mongolia grasslands. There was a significantly positive correlation between the drought indicators output by the model and the standardized precipitation evapotranspiration index (SPEI) measured in the field. The correlation coefficients (R) between the drought degree were 0.9706 and 0.6387 for the training set and test set, respectively. The consistent rate between the model drought index and the SPEI reached 87.90%. Drought events in Inner Mongolia were monitored from April to September in wet years, normal years, and dry years using the constructed model. The monitoring results of the model constructed in this study were in accordance with the actual drought conditions, reflecting the development and spatial evolution of drought conditions. This study provides a new application method for the comprehensive assessment of grassland drought.
作者机构：
[Yan, Xiaoqin; Chen, Xi; Wang, Guozhen; Ding, Wenping] Wuhan Polytech Univ, Sch Food Sci & Engn, Wuhan 430023, Peoples R China.;[Wang, Bowen] Beijing Technol & Business Univ, Beijing Lab Food Qual & Safety, Beijing 100048, Peoples R China.;[Hu, Xinping] Angel Yeast Co Ltd, Yichang, Hubei Province, Peoples R China.;[Wang, Guozhen] Wuhan Polytech Univ, Wuhan 430023, Peoples R China.
通讯机构：
[Guozhen Wang] S;[Bowen Wang] B;Beijing Laboratory of Food Quality and Safety, Beijing Technology and Business University, Beijing, 100048, China<&wdkj&>School of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, 430023, PR China
摘要：
To investigate the effects of milling methods on the properties of rice flour and steamed rice cakes (SRCs), dry, semi-dry, and wet milling methods were compared. Damaged starch, particle size distribution, water hydration properties, gelatinization properties, gel strength, thermal properties, glass transition temperature, dynamic rheological properties of rice flour, sensory and textural properties of SRCs were evaluated. Semidry-milling rice flour (SF) had much less damaged starch and smaller particle size compared with dry-milling rice flour (DF). The pasting, hydration, and rheological properties of SF were similar to that of wet-milling rice flour (WF). Except for adhesion and appearance, the sensory and textural properties of SF SRCs were significantly better than that of DF SRCs, while comparable to WF SRCs. The results showed that semi-dry milling as an environmental, efficient, and convenient method could provide rice flour suitable to prepare SRCs. In view of sustainable and economic development, semi-dry milling could be a good alternative method in industrial production.
期刊：
Nonlinear Analysis: Real World Applications,2022年63:103411 ISSN：1468-1218
通讯作者：
Hou, Meichen
作者机构：
[Hou, Meichen] Northwest Univ, Sch Math, Xian 710069, Peoples R China.;[Hou, Meichen] Northwest Univ, CNS, Xian 710069, Peoples R China.;[Fan, Lili] Wuhan Polytech Univ, Sch Math & Comp Sci, Wuhan 430023, Peoples R China.
通讯机构：
[Hou, Meichen] N;Northwest Univ, Sch Math, Xian 710069, Peoples R China.;Northwest Univ, CNS, Xian 710069, Peoples R China.
关键词：
Inflow problem;Non-viscous;Contact wave
摘要：
This paper is devoted to studying the inflow problem governed by the non-viscous and heat-conductive gas dynamic system in the one-dimensional half space. We establish the unique global-in-time existence and the asymptotic stability of the viscous contact wave. The contact discontinuity in the linearly degenerate field is less stable, and the dissipative mechanism for non-viscous systems is also weaker than that of viscous systems, these all make the problem more challenging. We used the weighted energy estimates to overcome those difficulties. Some technical discussions were created carefully by taking good advantage of properties of the supersonic region and the viscous contact wave. (C) 2021 Elsevier Ltd. All rights reserved.
通讯机构：
[Yang, Qing] I;[Zeng, Hao-Long] D;Department of Laboratory Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, People's Republic of China.;Institute of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, 430030, People's Republic of China.
摘要：
BACKGROUND: Adrenal tumors, including benign cortical adenoma (BCA) and pheochromocytoma (PCC) or paraganglioma (PGL), have been more frequently detected during imaging examinations in recent years. However, the associated clinical or laboratory characteristics, especially on the Chinese population, still need to be investigated. METHODS: We conducted a retrospective analysis of 491 patients pathologically diagnosed with adrenal tumors, from Jan 19, 2018 to Dec 17, 2019, at a tertiary referral hospital in Wuhan of China. Our findings including 247 (50.3%) BCA cases, and 92 (18.7) PCC/PGL cases and other cases. Both the clinical and laboratory parameters were reviewed and analyzed. RESULTS: Compared with other adrenal tumors, PCC/PGL showed larger tumor diameters and more frequently located on the right side, and were with higher levels of urinary catecholoamines and plasma metanephrines, especially for the 24h urinary vanilmandelic acid (VMA) and plasma normetanephrine (NMN). The optimal diagnostic thresholds were 29.40 ug/24h for VMA (sensitivity, 85%; specificity, 91%) and 0.63nmol/L for NMN (sensitivity, 91%; specificity, 92%). The 24h urinary VMA and plasma NMN also shared abilities to differentiate between different tumor laterality and different tumor size in PCC/PGL cases. In addition, compared with the other benign tumors, BCA were smaller in diameters (20 vs 35mm, p < 0.001), and seemed to be lower in levels of plasma epinephrine, dopamine and serum ACTH. CONCLUSION: 24h Urinary catecholoamines and plasma metanephrines, especially for the 24h urinary VMA and plasma MNM, showed higher diagnostic efficacies for PCC/PGL, and were tightly associated with the tumor laterality and tumor size.
作者机构：
[Cheng, Shuiyuan; He, Yi; Dong, Xingxing; Wang, Zhangqian; Gao, Chao; Li, Mengjun; Wang, Yidan; Zhang, Sishang; Deng, Jieya; Duan, Yuhua] Wuhan Polytech Univ, Natl R&D Ctr Se Rich Agr Prod Proc, Sch Modern Ind Selenium Sci & Engn, Wuhan, Peoples R China.;[Wang, Zhangqian; Wang, Qin] Hubei Univ, Sch Life Sci, State Key Lab Biocatalysis & Enzyme Engn, Wuhan 430062, Peoples R China.;[Yi, Tian] Hubei Acad Agr Sci, Inst Agr Qual Stand & Testing Technol Res, Wuhan 430064, Peoples R China.
通讯机构：
[Chao Gao; Zhangqian Wang] A;Authors to whom correspondence should be addressed.<&wdkj&>National R&D Center for Se-Rich Agricultural Products Processing, School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China<&wdkj&>Authors to whom correspondence should be addressed.<&wdkj&>State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan 430062, China<&wdkj&>National R&D Center for Se-Rich Agricultural Products Processing, School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China
摘要：
Selenium is an essential microelement required for human health. The biotransformation of selenium nanoparticles has attracted increasing attention in recent years. However, little of the literature has investigated the comprehensive evaluation of the strains for practical application and the effect on the functional properties in the existence of Se. The present study showed the selenite reduction strain Bacillus subtilis T5 (up to 200 mM), which could produce high yields of selenium polysaccharides and selenium nanoparticles in an economical and feasible manner. Biosynthesized selenium nanoparticles by B. subtilis T5 were characterized systematically using UV-vis spectroscopy, FTIR, Zeta Potential, DLS, and SEM techniques. The biosynthesized SeNPs exhibited high stability with small particle sizes. B. subtilis T5 also possessed a tolerance to acidic pH and bile salts, high aggregation, negative hemolytic, and superior antioxidant activity, which showed excellent probiotic potential and can be recommended as a potential candidate for the selenium biopharmaceuticals industry. Remarkably, B. subtilis T5 showed that the activity of alpha-amylase was enhanced with selenite treatment to 8.12 U/mL, 2.72-fold more than the control. The genus Bacillus was first reported to produce both selenium polysaccharides with extremely high Se-content (2.302 g/kg) and significantly enhance the activity to promote alpha-amylase with selenium treatment. Overall, B. subtilis T5 showed potential as a bio-factory for the biosynthesized SeNPs and organ selenium (selenium polysaccharide), providing an appealing perspective for the biopharmaceutical industry.
