期刊:
International Journal of Biological Macromolecules,2024年:128892 ISSN:0141-8130
通讯作者:
Chang, Chao;Wu, Jine
作者机构:
[Wu, Jine; Chang, Chao; Gu, Xiaolian; Jiang, Xiaoyu; Li, Wanbing] College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China;[Chang, Chao] Key Laboratory of Intensive Processing of Staple Grain and Oil, Ministry of Education, Key Laboratory for Processing and Transformation of Agricultural Products, Wuhan Polytechnic University, Wuhan 430023, Hubei, China. Electronic address: changchao2000@163.com;[Wu, Jine] Key Laboratory of Intensive Processing of Staple Grain and Oil, Ministry of Education, Key Laboratory for Processing and Transformation of Agricultural Products, Wuhan Polytechnic University, Wuhan 430023, Hubei, China. Electronic address: wujinec@163.com
通讯机构:
[Wu, Jine; Chang, Chao] K;Key Laboratory of Intensive Processing of Staple Grain and Oil, Ministry of Education, Key Laboratory for Processing and Transformation of Agricultural Products, Wuhan Polytechnic University, Wuhan 430023, Hubei, China. Electronic address:
关键词:
Adding sequence;Curcumin;Heating pH;Self-aggregated;Zein/whey protein isolate (WPI)/pectin nanoparticles
摘要:
In this work, pectin was employed as a coating material to fabricate zein/whey protein isolate (WPI)/pectin complex nanoparticles via a pH-adjusted and heat-induced electrostatic adsorption process for potential oral administration applications of curcumin. Factors such as the order of raw material addition, heating temperature and pH, and zein concentration were comprehensively examined. In addition to electrostatic interactions, Fourier transform infrared and fluorescence spectroscopy indicated that hydrophobic interactions and hydrogen bonds were also involved in the development of complex nanoparticles. The complex nanoparticles obtained not only improved the antioxidant activity of curcumin in aqueous phase, but also contributed to its controlled release under gastrointestinal conditions. Our findings revealed that the heating pH and adding sequence of raw materials had a notable impact on the properties of complex nanoparticles, and that pectin coating had an exceptional stabilizing effect on complex nanoparticles under gastrointestinal circumstances. This study provides novel insights and perspectives for the preparation of polysaccharide-protein complex nanoparticles, signifying the potential use of zein/WPI/pectin complex nanoparticles as delivery vehicles in the functional food and pharmaceutical industries.
作者机构:
[Zhang, Qi; Ge, Feng; Yang, Mingkun; Zhao, Jindong; Jia, Kun; Liu, Xin; Cao, Gaoxiang] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China.;[Zhang, Qi; Ge, Feng; Yang, Mingkun; Jia, Kun; Cao, Gaoxiang] Univ Chinese Acad Sci, Coll Adv Agr Sci, Beijing 100049, Peoples R China.;[Liu, Xin] Wuhan Polytech Univ, Sch Anim Sci & Nutr Engn, Wuhan 430070, Peoples R China.;[Zhao, Jindong] Peking Univ, Coll Life Sci, State Key Lab Prot & Plant Genet Engn, Beijing 100871, Peoples R China.
通讯机构:
[Ge, F; Zhao, JD ] C;[Ge, F ] U;Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China.;Univ Chinese Acad Sci, Coll Adv Agr Sci, Beijing 100049, Peoples R China.;Peking Univ, Coll Life Sci, State Key Lab Prot & Plant Genet Engn, Beijing 100871, Peoples R China.
摘要:
Lysine acetylation is a conserved regulatory posttranslational protein modification that is performed by lysine acetyltransferases (KATs). By catalyzing the transfer of acetyl groups to substrate proteins, KATs play critical regulatory roles in all domains of life; however, no KATs have yet been identified in cyanobacteria. Here, we tested all predicted KATs in the cyanobacterium Synechococcus sp. PCC 7002 (Syn7002) and demonstrated that A1596, which we named cyanobacterial Gcn5-related N-acetyltransferase (cGNAT2), can catalyze lysine acetylation in vivo and in vitro. Eight amino acid residues were identified as the key residues in the putative active site of cGNAT2, as indicated by structural simulation and site-directed mutagenesis. The loss of cGNAT2 altered both growth and photosynthetic electron transport in Syn7002. In addition, quantitative analysis of the lysine acetylome identified 548 endogenous substrates of cGNAT2 in Syn7002. We further demonstrated that cGNAT2 can acetylate NAD(P)H dehydrogenase J (NdhJ) in vivo and in vitro, with the inability to acetylate K89 residues, thus decreasing NdhJ activity and affecting both growth and electron transport in Syn7002. In summary, this study identified a KAT in cyanobacteria and revealed that cGNAT2 regulates growth and photosynthesis in Syn7002 through an acetylation-mediated mechanism. The cyanobacterial Gcn5-related N-acetyltransferase regulates the growth and photosynthesis of Synechococcus PCC 7002 through an acetylation-mediated mechanism.
摘要:
The application of rapeseed protein in human foods is limited by residual antinutritive components and poor sensory quality. The effects of five extraction protocols on rapeseed protein yield, sensory, functional and nutritional properties were systematically evaluated in this study. In particular, the potential of weakly acidic salt (pH 6.5, 150mmol·L(-1) MgCl(2)) extraction as a mild method for recovering edible rapeseed protein was investigated compared with conventional alkali extraction. All salt-extracted proteins showed above 40% extraction yield and low antinutritional factor contents. They also had ideal amino acid patterns and better in vitro gastroduodenal digestibility than alkaline-extracted proteins. Additionally, the lighter color and odor, as well as better solubility, emulsion activity, foaming property, and water/oil holding capacity were found in weakly acidic salt extraction-ultrafiltered proteins. These findings suggest that weakly acidic salt extraction-ultrafiltration could be used for obtaining edible rapeseed protein, while extraction yield should be improved for scale application.
摘要:
Although tremendous progress has recently been made in multi-walled carbon nanotubes (MWCNT) and titania, the photocatalytic degradation efficiency is still greatly limited by the ideal cobalt dopant concentration and temperature. Herein, Co-CNT/TiO2 composites were used to improve the photocatalytic degradation efficiency of malachite green (MG) solution. Consequently, compared to the binary CNT/TiO2 and pure TiO2 samples, the ternary Co-CNT/TiO2 samples showed much better photocatalytic activity. The sample calcined at 500 degrees C showed better reactivity, and the ideal cobalt dopant concentration for the highest photocatalytic degradation was 0.5%.
摘要:
Selenium-enriched Cardamine violifolia (SEC), a cruciferous plant, exerts excellent antioxidant and anti-inflammatory capacity, but its effect on hepatic function is unclear. This study investigated the effect and potential mechanism of SEC on hepatic injury induced by lipopolysaccharide (LPS). Twenty-four weaned piglets were randomly allotted to treatment with SEC (0.3 mg/kg Se) and/or LPS (100 mu g/kg). After 28 days of the trial, pigs were injected with LPS to induce hepatic injury. These results indicated that SEC supplementation attenuated LPS-induced hepatic morphological injury and reduced aspartate aminotransferase (AST) and alkaline phosphatase (ALP) activities in plasma. SEC also inhibited the expression of pro-inflammatory cytokines such as interleukin 6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha) after the LPS challenge. In addition, SEC improved hepatic antioxidant capacity via enhancing glutathione peroxidase (GSH-Px) activity and decreasing malondialdehyde (MDA) concentration. Moreover, SEC downregulated the mRNA expression of hepatic myeloid differentiation factor 88 (MyD88) and nucleotide-binding oligomerization domain proteins 1 (NOD1) and its adaptor molecule receptor interacting protein kinase 2 (RIPK2). SEC also alleviated LPS-induced hepatic necroptosis by inhibiting RIPK1, RIPK3, and mixed-lineage kinase domain-like (MLKL) expression. These data suggest that SEC potentially mitigates LPS-induced hepatic injury via inhibiting Toll-like receptor 4 (TLR4)/NOD2 and necroptosis signaling pathways in weaned piglets.
期刊:
Science of The Total Environment,2024年912:168859 ISSN:0048-9697
通讯作者:
Wang, Qiao
作者机构:
[Liu, Yan; Peng, Qiuhong; Gong, Zhiyong; Duan, Shuo; Li, Yuzhi; Wang, Qiao; Liu, Jin; Yin, Jiaojiao; Liu, Xin; Peng, Lingfeng] College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China;[Liu, Yan; Gong, Zhiyong; Duan, Shuo; Yin, Jiaojiao; Liu, Xin] Key Laboratory for Deep Processing of Major Grain and Oil Ministry of Education, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, Wuhan Polytechnic University, Wuhan 430023, China;[Liu, Xiaofang] Hubei Provincial Key Laboratory for Applied Toxicology, Hubei Provincial Center for Disease Control and Prevention, Wuhan 430079, China;[Li, Yuzhi] Key Laboratory of Detection Technology of Focus Chemical Hazards in Animal-derived Food for State Market Regulation, Wuhan 430075, China;[Wang, Qiao] Key Laboratory for Deep Processing of Major Grain and Oil Ministry of Education, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, Wuhan Polytechnic University, Wuhan 430023, China. Electronic address: wangqiao8577@163.com
通讯机构:
[Wang, Qiao] K;Key Laboratory for Deep Processing of Major Grain and Oil Ministry of Education, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, Wuhan Polytechnic University, Wuhan 430023, China. Electronic address:
摘要:
Organophosphate flame retardants (OPFRs), a novel class of persistent pollutants, are widely distributed in the environment, and their potential health risks have garnered significant global attention in recent years. Crayfish is a popular freshwater crustacean product in China primarily sourced from the middle and lower reaches of the Yangtze River. The purpose of this study was to investigate the exposure levels of OPFRs in crayfish, assess the health and safety risks associated with crayfish consumption, and explore the bioaccumulation of OPFRs in environmental water and sediment on crayfish. Ultra-high performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was employed to analyze 7 common OPFRs in 106 crayfish samples and 76 environmental samples. The results revealed that OPFRs were detected at a high frequency of 100% in crayfish, with tripropyl phosphate (TPP) being the predominant pollutant found in edible portions while also exhibiting secondary contamination within the crayfish food chain. Monte Carlo modeling combined with @risk risk assessment software demonstrated that TPP present in crayfish muscles had the most substantial impact on health effects, however, overall OPFR exposure did not pose significant risks to human health. Furthermore, analysis of OPFRs bioenrichment ability indicated that crayfish predominantly accumulated these compounds within their edible parts from surrounding environmental water sources, particularly highlighting TPP's potential for bioaccumulation.
摘要:
Latex clearing protein from Streptomyces sp. strain K30 (Lcp(K30)) is a natural oxidoreductase that can catalyse the cleavage of rubber through dioxygenation. It has significant potential applications in polymer degradation. However, its limited expression in engineered strains restricts its utility. This study aimed to enhance the soluble expression and enzyme activity of Lcp(K30) in E. coli BL21 (DE3) by optimizing fermentation conditions and making molecular modifications. The enzyme activity reached 5.05U·mL(-1) by optimizing the induction conditions, adding cofactors, and using chemical chaperones, which was 237.1% of the initial case. Further enhancements in soluble expression were achieved through site mutations guided by the PROSS server, resulting in 8 out of 13 mutants with increased protein expression, a high positive mutation rate of 61.5%. Subsequently, combined mutants were created by merging single mutants with enhanced protein expression and enzyme activity. The top three double mutants, G91D/S149A, G91D/A210H, and G91D/H296P, displayed expression levels at 173.3%, 173.3%, and 153.3% of the wild-type Lcp(K30), respectively. These mutants also exhibited enhanced fermentation enzyme activity, reaching 149.5%, 250.0%, and 420.2% compared to the wild-type, along with improved specific activities. This study provides insights for the efficient production of Lcp(K30) and a practical foundation for its application.
通讯机构:
[Tang, ZY ] N;[Liu, Y ; Guo, J] T;Tiangong Univ, Sch Chem, State Key Lab Separat Membranes & Membrane Proc, Tianjin 300387, Peoples R China.;Natl Ctr Nanosci & Technol, CAS Ctr Excellence Nanosci, CAS Key Lab Nanosyst & Hierarch Fabricat, Beijing 100190, Peoples R China.
摘要:
Highly enantioretentive alcoholysis of epoxides is an important way to synthesize enantiopure beta-alkoxy alcohols, which are irreplaceable intermediates demanded by biomedicines, fine chemicals and other industries. In this report, we exploit a series of Zr-based metal-organic frameworks (Zr-MOFs) as the catalysts to achieve high activity and enantioretentivity in the alcoholysis of styrene oxide via modulating their assembly fashions. It is explored that hcp-UiO-66 not only exhibits a similar to 10 fold improved catalytic activity than both hxl-CAU-26 and fcc-UiO-66 of varied assemblies but also maintains superior product enantioretentivity. Theoretic calculations together with experimental proof discloses the origin of distinct catalytic activity caused by different assembly fashions. This assembly modulation strategy offers a potential protocol for seeking high-performance catalysts among MOFs by virtue of their rich polymorphisms. Modulation of the assembly fashion among metal-organic frameworks enabling efficient enantioretentive conversion of epoxide.
摘要:
Abstract Botrytis cinerea is a necrotrophic fungal plant pathogen that causes grey mould and rot diseases in many crops. Here, we show that the B. cinerea BcCrh4 transglycosylase is secreted during plant infection and induces plant cell death and pattern‐triggered immunity (PTI), fulfilling the characteristics of a cell death‐inducing protein (CDIP). The CDIP activity of BcCrh4 is independent of the transglycosylase enzymatic activity, it takes place in the apoplast and does not involve the receptor‐like kinases BAK1 and SOBIR1. During saprophytic growth, BcCrh4 is localized in the endoplasmic reticulum and in vacuoles, but during plant infection, it accumulates in infection cushions (ICs) and is then secreted to the apoplast. Two domains within the BcCrh4 protein determine the CDIP activities: a 20aa domain at the N′ end activates intense cell death and PTI, while a stretch of 52aa in the middle of the protein induces a weaker response and suppresses the activity of the 20aa N′ domain. Deletion of bccrh4 affected fungal development and IC formation in particular, resulting in reduced virulence. Collectively, our findings demonstrate that BcCrh4 is required for fungal development and pathogenicity, and hint at a dual mechanism that balances the virulence activity of this, and potentially other CDIPs.
摘要:
Solid‐state fermentation of faba bean (Vicia faba L.) meal. Summary Solid‐state fermentation (SSF) is widely used to improve the utility value in the food and feed industries. In the present research, Lactobacillus plantarum and Saccharomycopsis fibuligera were used for SSF, and then, the nutritional values, anti‐nutritional factors (ANFs) and antioxidant activities of faba bean meal (FBM) were comparatively analysed. The results revealed that SSF with S. fibuligera and L. plantarum effectively reduce the tannin and phytic acid contents and improve the nutritional quality and antioxidant capacity in FBM. Meanwhile, the effects of SSF with L. plantarum on degrading tannin and increasing titratable acidity, trichloroacetic acid‐soluble protein and in vitro protein digestibility were better treated with S. fibuligera. However, antioxidant activities and the amount of phenol and flavonoid were improved more in FBM treated with S. fibuligera than those fermented with L. plantarum. Taken together, the results indicated that both L. plantarum and S. fibuligera could be used for SSF of FBM, and SSF with those two microorganisms has great potential in improving the nutritional values, antioxidant capacities and decreasing ANFs contents of FBM.
摘要:
A series of cross-linked AEMs (c-DQPPO/PVA) are synthesized by using rigid polyphenylene oxide and flexible poly(vinyl alcohol) as the backbones. Dual cations are grafted on the PPO backbone to improve the ion exchange capacity (IEC), while glutaraldehyde is introduced to enhance compatibility and reduce swelling ratio of AEMs. In addition to the enhanced mechanical properties resulting from the rigid-flexible cross-linked network, c-DQPPO/PVA AEMs also exhibit impressive ionic conductivity, which can be attributed to their high IEC, good hydrophilicity of PVA, and well-defined micro-morphology. Additionally, due to confined dimension behavior and ordered micro-morphology, c-DQPPO/PVA AEMs demonstrate excellent chemical stability. Specifically, c-DQPPO/PVA-7.5 exhibits a wet-state tensile strength of 12.5 MPa and an elongation at break of 53.0 % at 25 degrees C. Its OH- conductivity and swelling degree at 80 degrees C are measured to be 125.7 mS cm(-1) and 8.2 %, respectively, with an IEC of 3.05 mmol g(-1). After 30 days in a 1 M NaOH solution at 80 degrees C, c-DQPPO/PVA-7.5 experiences degradation rates of 12.8 % for tensile strength, 27.4 % for elongation at break, 14.7 % for IEC, and 19.2 % for ion conductivity. With its excellent properties, c-DQPPO/PVA-7.5 exhibits a peak power density of 0.751 W cm(-2) at 60 degrees C in an H-2-O-2 fuel cell.
期刊:
Journal of Materials Science,2024年 ISSN:0022-2461
通讯作者:
Li, B
作者机构:
[Wu, Aolin; Li, B; Li, Bin; Su, Lihua; Liu, Haiying; Du, Li; Zhou, Mengjing] Wuhan Polytech Univ, Sch Mech Engn, Wuhan 430023, Hubei, Peoples R China.
通讯机构:
[Li, B ] W;Wuhan Polytech Univ, Sch Mech Engn, Wuhan 430023, Hubei, Peoples R China.
摘要:
Preparation of composite hydrogels by in situ polymerization (SP) using choline chloride (ChCl), acrylamide (AM), and urea (U) as raw materials for deep eutectic solvent (DES), with the introduction of PVA and PVDF as fillers into DES. Characterization of the composite hydrogels was conducted using Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). This study analyzed the pressure sensitivity, piezoelectricity, swelling behavior, and mechanical properties of the composite hydrogels. The results indicate that due to the dipole-dipole interactions between PVA and PVDF, the composite hydrogels exhibit excellent pressure sensitivity and piezoelectric performance. When the ratio of PVA to PVDF is 1:1, the composite hydrogels exhibit optimal performance, with a 3.37-fold increase in pressure sensitivity and a 6.49-fold increase in piezoelectricity compared to other ratios. This research provides a method for preparing composite hydrogels with excellent piezoelectric performance and pressure sensitivity.
摘要:
Polyaniline-dodecylbenzene sulfonic acid (PANI-DBSA) particles were synthesized through in situ lotion polymerization of aniline and DBSA as raw materials, followed by combining with epoxy resin (E-44) and castor oil (CO) to prepare a PANI mixture (PANI-DBSA/CO) which was used as a nanofiller to create a nano polyaniline/polyurethane (PANI-PU) interpenetrating polymer network (IPN) coating. SEM, XRD and Fourier transform infrared spectroscopy were used to characterize the PANI-DBSA and PANI-PU IPN composite coatings that were produced. The coatings' physical and mechanical characteristics were investigated, as well as the system's corrosion behaviour in 3.5 wt% NaCl solution, utilizing electrochemical impedance spectroscopy and electrokinetic polarization. Acid, alkali and saltwater immersion investigations were used to further evaluate the coatings' medium resistance. The results show that when PANI-DBSA is added at 1 wt%, the contact angle reaches 102.6 degrees, the adhesion reaches S1 grade after 720 h immersion, the tensile strength is 16.22 MPa and the elongation reaches 208%. The impedance value of the composite coating grew by 569 times with the addition of 1 wt% PANI, and the resistance duration to 10 wt% H2SO4, 10 wt% NaOH and 3.5 wt% NaCl reached more than 90 days. The addition of PANI to IPN coatings can increase corrosion resistance and extend their application range. (c) 2023 Society of Industrial Chemistry.
期刊:
International Journal of Biological Macromolecules,2024年:127864 ISSN:0141-8130
通讯作者:
Wang, Haibo
作者机构:
[Xu, Chengzhi; Hu, Wenjing; Wei, Benmei; Zhu, Lian; Kou, Huizhi; Zhang, Juntao] School of Chemistry and Environmental Engineering, Wuhan Polytechnic University, Wuhan, Hubei, China;[Xiao, Xiao] School of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, Hubei, China;[Wang, Haibo] College of Life Science and Technology, Hubei Key Laboratory of Quality Control of Characteristic Fruits and Vegetables, Hubei Engineering University, Xiaogan, Hubei, China. Electronic address: wanghaibo@whpu.edu.cn
通讯机构:
[Wang, Haibo] C;College of Life Science and Technology, Hubei Key Laboratory of Quality Control of Characteristic Fruits and Vegetables, Hubei Engineering University, Xiaogan, Hubei, China. Electronic address:
摘要:
Customized control of the biological response between the material matrix and cells is a crucial aspect in the development of the next generation of collagen materials. This study aims to investigate the effects of ultrahigh pressure treatment on the interaction between collagen and cells by subjecting bovine tendon collagen to different intensities of ultrahigh pressure field. The results indicate that ultrahigh pressure treatment alters the spatial folding of collagen, causing distortion of its triple helical conformation and exposing more free amino groups and hydrophobic regions. As a result, collagen's cell adhesion capability and ability to promote cell migration are significantly enhanced. Optimal cell adhesion and migration capabilities are observed in collagen samples treated at 500 MPa for 15 min. However, further increasing the intensity of the ultrahigh pressure treatment leads to severe damage to the triple-helical structure of collagen, along with re-aggregation of free amino groups and hydrophobic moieties, thereby reducing collagen's cell adhesion capability and ability to promote cell migration. Therefore, ultrahigh pressure treatment offers a promising method to effectively regulate collagen-cell adhesion and promote cell migration without the need for external components. This provides a potential means for the customized enhancement of collagen-based material interfaces.
作者机构:
[Wang, Yong] Department of Civil and Environmental Engineering, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China. Electronic address: ryan-yong.wang@polyu.edu.hk;[Sun, Xiaohao] Department of Civil and Environmental Engineering, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China. Electronic address: xiaohsun@polyu.edu.hk;[Miao, Linchang] Institute of Geotechnical Engineering, Southeast University, Nanjing 210096, Jiangsu, China. Electronic address: Lc.miao@seu.edu.cn;[Wang, Hengxing] Institute of Geotechnical Engineering, Southeast University, Nanjing 210096, Jiangsu, China. Electronic address: 230198266@seu.edu.cn;[Wu, Linyu] School of Civil Engineering and Architecture, Wuhan Polytechnic University, Wuhan 430023, Hubei, China. Electronic address: 22113073@whpu.edu.cn
通讯机构:
[Sun, Xiaohao; Wang, Yong] D;[Wang, Hengxing; Miao, Linchang] I;[Kawasaki, Satoru] F;[Wu, Linyu] S;Department of Civil and Environmental Engineering, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China. Electronic address: ryan-yong.
关键词:
EICP;Factors to improve resistance;MICP;Prospective applications;Soil erosion
摘要:
In recent years, the application of microbially induced calcite precipitation (MICP) and enzyme-induced carbonate precipitation (EICP) techniques have been extensively studied to mitigate soil erosion, yielding substantial achievements in this regard. This paper presents a comprehensive review of the recent progress in erosion control by MICP and EICP techniques. To further discuss the effectiveness of erosion mitigation in-depth, the estimation methods and characterization of erosion resistance were initially compiled. Moreover, factors affecting the erosion resistance of MICP/EICP-treated soil were expounded, spanning from soil properties to treatment protocols and environmental conditions. The development of optimization and upscaling in erosion mitigation via MICP/EICP was also included in this review. In addition, this review discussed the limitations and correspondingly proposed prospective applications of erosion control via the MICP/EICP approach. The current review presents up-to-date information on the research activities for improving erosion resistance by MICP/EICP, aiming at providing insights for interdisciplinary researchers and guidance for promoting this method to further applications in erosion mitigation.