关键词：
Near -Infrared spectroscopy;Rapid non-destructive detection;Grass carp fillets;Total volatile basic nitrogen;Machine learning;Orthogonal signal correction (OSC);Particle swarm optimization algorithm;optimized BP neural network (PSO-BP)
摘要：
This research aims to accurately forecast the freshness indicators (TVB-N) of skinned and skinless grass carp fillets by integrating near-infrared spectroscopy (NIR) with machine learning algorithms. By comparing the predictive accuracy of machine learning models for the two types of grass carp fillets, the most effective modeling method is identified. Methodologically, the study first applies orthogonal signal correction (OSC) and the first derivative among other algorithms for spectral pre-processing. Subsequently, competitive adaptive reweighted sampling (CARS), moving window partial least squares (MWPLS), and random frog (RF) are used for the selection of variables. Lastly, partial least squares regression (PLSR), support vector regression (SVR), backpropagation neural networks (BPNN), and particle swarm optimization-enhanced BP neural networks (PSO-BP) are employed to quantitatively analyze the NIR data. The most relevant results reveal that the (OSC+D1)-CARS-PSO-BP model exhibits superior predictive capabilities. Especially when applied to skin-on fish fillets (R2P =0.988, RMSEP=0.092), this model surpasses that for skinless fish fillet data (R2P =0.987, RMSEP=0.096). Therefore, combining near-infrared with machine learning to predict the freshness (TVB-N) of grass carp fillets based on skin-on samples is a more effective non-destructive testing method.
通讯机构：
[Shen, SN ; Li, H] W;Wuhan Univ, Inst Technol Sci, Wuhan 430072, Peoples R China.
关键词：
Laser powder bed fusion;Laser ultrasonic;Thermo-elastic regime;Internal defect
摘要：
In the development of additive manufacturing (AM) technology, laser powder bed fusion (LPBF) is one of the important processing methods. However, the hole defects in the fabricated samples limit the development. Laser ultrasonic (LU) technology plays a major role in the detection of LPBF parts with tiny defects, which has the advantages of non-contact and non-destructive. In this work, the detection of submillimeter internal defects in four typical LPBF alloys by LU technology is studied numerically and experimentally. A multiphysics simulation model of LU detection is established to investigate the propagation characteristics of excited ultrasonic waves in different LPBF alloys and their interaction with submillimeter artificial defects. Simulation results show that the amplitude of longitudinal (L) wave at the defect is the largest in AlSi10Mg alloy, and the amplitude of L wave in the 316L alloy, Ti6Al4V alloy and In718 alloy are very close, but their phases are slightly different. The amplitude of L wave tends to decrease nearly linearly with the increase in defect diameter. Then, four typical LPBF alloys are fabricated and measured by the LU through-transmission detection. The geometric information of artificial holes with a diameter larger than 0.2 mm are clearly characterized by the LU C-scan results, indicating the prominent applicability and feasibility of LU detection on different materials fabricated by LPBF.
作者机构：
[Wang, Baoxin; Zhang, Jing; Zheng, Hao; Dong, Xia; Zhou, Ao; Zhang, Wenhua; Chen, Hongbo] School of Animal Science and Nutritional Engineering, Laboratory of Genetic Breeding, Reproduction and Precision Livestock Farming, Wuhan Polytechnic University, Wuhan 430023, Hubei, China;[Wang, Baoxin; Zhang, Jing; Zheng, Hao; Dong, Xia; Zhou, Ao; Zhang, Wenhua; Chen, Hongbo] Hubei Provincial Center of Technology Innovation for Domestic Animal Breeding, Wuhan 430023, Hubei, China;[Wu, Junjing] Hubei Key Laboratory of Animal Embryo and Molecular Breeding, Institute of Animal Husbandry and Veterinary, Hubei Provincial Academy of Agricultural Sciences, Wuhan, China
关键词：
Introduction;Materials and Methods;Results;Discussion;Conclusion;Abstract;Data Availability;Additional Points;Ethical Approval;Consent;Disclosure;Conflicts of Interests;Authors’ Contributions;Funding Statement;Acknowledgements;Acknowledgments;Supplementary Materials;Reference;Dataset Description;Dataset Files;Abstract;Introduction;Introduction and Materials;Introduction and Methods;Materials;Materials and Methods;Methods;Results;Discussion;Results and Discussion;Discussion and Conclusion;Results and Conclusion;Conclusion;Conclusions;Data Availability;Additional Points;Ethical Approval;Consent;Disclosure;Conflicts of Interest;Authors’ Contributions;Funding Statement;Acknowledgements;Supplementary Materials;References;Appendix;Abbreviations;Preliminaries;Introduction and Preliminaries;Notation;Proof of Theorem;Proofs;Analysis of Results;Examples;Numerical Example;Applications;Numerical Simulation;Model;Model Formulation;Systematic Palaeontology;Nomenclatural Acts;Taxonomic Implications;Experimental;Synthesis;Overview;Characterization;Background;Experimental;Theories;Calculations;Model Verification;Model Implementation;Geographic location;Study Area;Geological setting;Data Collection;Field Testing;Data and Sampling;Dataset;Literature Review;Related Works;Related Work;System Model;Methods and Data;Experimental Results;Results and Analysis;Evaluation;Implementation;Case Presentation;Case Report;Search Terms;Case Description;Case Series;Background;Limitations;Additional Points;Case;Case 1;Case 2 etc.;Concern Details;Retraction Details;Copyright;Related Articles
摘要：
Influenza pandemic with H1N1 (H1N1pdms) causes severe lung damage and "cytokine storm," leading to higher mortality and global health emergencies in humans and animals. Explaining host antiviral molecular mechanisms in response to H1N1pdms is important for the development of novel therapies. In this study, we organised and analysed multimicroarray data for mouse lungs infected with different H1N1pdm and nonpandemic H1N1 strains. We found that H1N1pdms infection resulted in a large proportion of differentially expressed genes (DEGs) in the infected lungs compared with normal lungs, and the number of DEGs increased markedly with the time of infection. In addition, we found that different H1N1pdm strains induced similarly innate immune responses and the identified DEGs during H1N1pdms infection were functionally concentrated in defence response to virus, cytokine-mediated signalling pathway, regulation of innate immune response, and response to interferon. Moreover, comparing with nonpandemic H1N1, we identified ten distinct DEGs (AREG, CXCL13, GATM, GPR171, IFI35, IFI47, IFIT3, ORM1, RETNLA, and UBD), which were enriched in immune response and cell surface receptor signalling pathway as well as interacted with immune response-related dysregulated genes during H1N1pdms. Our discoveries will provide comprehensive insights into host responding to pandemic with influenza H1N1 and find broad-spectrum effective treatment.
通讯机构：
[Sprunck, S ] U;[Xiong, HX ; Sun, MX ] W;Wuhan Univ, Coll Life Sci, State Key Lab Hybrid Rice, Wuhan, Peoples R China.;Univ Regensburg, Cell Biol & Plant Biochem, Regensburg, Germany.;Wuhan Polytech Univ, Sch Life Sci & Technol, Wuhan, Peoples R China.
摘要：
During double fertilization in angiosperms, the pollen tube delivers two sperm cells into an embryo sac; one sperm cell fuses with an egg cell, and the other sperm cell fuses with the central cell. It has long been proposed that the preference for fusion with one or another female gamete cell depends on the sperm cells and occurs during gamete recognition. However, up to now, sperm-dependent preferential fertilization has not been demonstrated, and results on preferred fusion with either female gamete have remained conflicting. To investigate this topic, we generated Arabidopsis thaliana mutants that produce single sperm-like cells or whose egg cells are eliminated; we found that although the three different types of sperm-like cell are functionally equivalent in their ability to fertilize the egg and the central cell, each type of sperm-like cell fuses predominantly with the egg cell. This indicates that it is the egg cell that controls its preferential fertilization. We also found that sperm-activating small secreted EGG CELL 1 proteins are involved in the regulation of egg-cell-dependent preferential fertilization, revealing another important role for this protein family during double fertilization. Preferential fertilization of the egg or central cell during double fertilization has long been controversial. The authors demonstrate preferential sperm–egg fusion in Arabidopsis and show that EGG CELL 1, which is secreted by the egg cell, plays a decisive role in this process.
通讯机构：
[Zhu, ZZ ; Li, SY ] W;Wuhan Polytech Univ, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.;Wuhan Polytech Univ, Key Lab Deep Proc Major Grain & Oil, Minist Educ, Wuhan 430023, Peoples R China.;Wuhan Polytech Univ, Natl R&D Ctr Se Rich Agr Prod Proc Technol, Wuhan 430023, Peoples R China.
关键词：
gluten protein;grape skin anthocyanin extract;microstructure and physicochemical properties
摘要：
Intake of polyphenol-modified wheat products has the potential to reduce the incidence of chronic diseases. In order to determine the modification effect of polyphenols on wheat gluten protein, the effects of grape skin anthocyanin extract (GSAE, additional amounts of 0.1%, 0.2%, 0.3%, 0.4%, and 0.5%, respectively) on the microstructure and physicochemical properties of gluten protein were investigated. The introduction of GSAE improves the maintenance of the gluten network and increases viscoelasticity, as evidenced by rheological and creep recovery tests. The tensile properties of gluten protein were at their peak when the GSAE level was 0.3%. The addition of 0.5% GSAE may raise the denaturation temperature of gluten protein by 6.48 °C-9.02 °C at different heating temperatures, considerably improving its thermal stability. Furthermore, GSAE enhanced the intermolecular hydrogen bond of gluten protein and promoted the conversion of free sulfhydryl groups to disulfide bonds. Meanwhile, the GSAE treatment may also lead to protein aggregation, and the average pore size of gluten samples decreased significantly and the structure became denser, indicating that GSAE improved the stability of the gluten spatial network. The positive effects of GSAE on gluten protein properties suggest the potential of GSAE as a quality enhancer for wheat products.
摘要：
In the practical operation of traditional landfills, compaction clay often experiences cracking, while the HDPE geomembrane may tear and bulge, resulting in a compromised performance of the landfill covering system. To address this issue, a capillary retarding covering material for landfill sites is proposed by utilizing municipal sludge and construction waste particles as substrates and incorporating a small quantity of calcium bentonite. The mechanical characteristics of the covering material were investigated using a standard consolidation test and a triaxial compression test. A permeability test and a soil water characteristic curve (SWCC) test were conducted to examine the permeability and capillary retarding effect of the covering material. Microscopic tests including SEM scanning, laser particle size analysis, and T2 NMR analysis were performed to investigate the connection mode, particle size composition, and pore structure characteristics of the covered particles. Based on the aforementioned research, the following conclusions can be drawn: The cohesion of the covering material ranged from 50 to 150 kPa, while the internal friction angle ranged from 24.23 degrees to 31 degrees. The cohesion was directly proportional to the content of construction waste, whereas the internal friction angle was inversely proportional to calcium bentonite content. The permeability coefficient ranged from 5.04 x 10-6 cm/s to 7.34 x 10-5 cm/s, indicating a certain level of impermeability. Both the sludge and the calcium bentonite contents jointly influenced the final permeability coefficient in a negative correlation manner, with a notable hydraulic hysteresis phenomenon observed. A higher content of construction waste leads to a more pronounced supporting force exerted by the formed skeleton structures within a load pressure range between 0 and 1600 kPa. When considering a mass ratio of municipal sludge: construction waste: calcium bentonite as 30:60:7, respectively, only a decrease in the pore ratio by approximately 13.20% was observed. This study provides valuable data support for designing and applying capillary retarding cover barrier systems in landfills.
摘要：
BACKGROUND: With the aging of human society, more and more elderly patients have to undergo surgery and anesthesia. Clinical observations have indicated from time to time that spinal anesthesia in the elderly appears to last longer than in young people, although there is limited research in this area and the mechanism is unclear at present time. This research work is expected to help understand the decline of local anesthetic metabolism in cerebrospinal fluid of elderly patients so as to help them with precise anesthesia and rapid rehabilitation. METHODS: Twenty patients with spinal anesthesia in orthopedic lower limb surgery were selected to study the rate of drug metabolism in cerebrospinal fluid in two age groups, i.e.,18-30years old and 75-90years old. Ropivacaine in peripheral blood is used as a probe to reflect the speed of drug metabolism in cerebrospinal fluid. The contents of total Aβ protein and hyaluronic acid in the cerebrospinal fluid were investigated as well. RESULTS: The equivalent dose of ropivacaine anesthetizes the elderly group for a longer time. The metabolism rate of ropivacaine in an elderly patient was slower than that of a young patient. No significant difference in total Aβ protein between the two groups was observed while hyaluronic acid in the elderly group was significantly higher than that in the young group. CONCLUSIONS: This study shows that the dose of ropivacaine should be reduced when used for anesthesia in elderly patients. The cumulation of ropivacaine and HA appears to imitate the degeneration of central lymphatic circulation metabolism in elderly people.
期刊：
Colloids and Surfaces A: Physicochemical and Engineering Aspects,2024年688:133671 ISSN：0927-7757
通讯作者：
Pengfei Jia
作者机构：
School of Electrical Engineering, Guangxi University, Nanning, China;School of Electrical and Electronic Engineering, Wuhan Polytechnic University, Wuhan, China;Guangxi Key Laboratory of intelligent Control and Maintenance of Power Equipment, Guangxi University, Nanning, China;[Jianjun Cao; Mingxiang Wang] School of Electrical Engineering, Guangxi University,Nanning,China<&wdkj&>Guangxi Key Laboratory of intelligent Control and Maintenance of Power Equipment, Guangxi University,Nanning,China;[Yiyi Zhang; Jiefeng Liu; Pengfei Jia] School of Electrical Engineering, Guangxi University,Nanning,China
通讯机构：
[Pengfei Jia] S;School of Electrical Engineering, Guangxi University, Nanning, China
摘要：
Insulation performance testing of gas-insulated switchgear (GIS) and treatment of SF6 decomposition products under partial discharge are two crucial studies. In this paper, the adsorption behavior and sensor properties of five characteristic decomposition gases of SF6 (HF, H2S, SO2, SOF2, SO2F2) on intrinsic and CoO-doped SnSe monolayers are investigated in detail based on density-functional theory. The results showed that the adsorption behaviors of HF, H2S, SOF2 and SO2F2 on SnSe monolayers were all physisorption among which the best adsorption was SO2 with an adsorption energy of −0.618 eV, which is a weak chemisorption. After doping with CoO, the adsorption capacity of SnSe monolayer for five gases was significantly enhanced, in which the adsorption energies for SO2 and SOF2 reached −1.356 eV and −1.175 eV, respectively. In addition, the conductivity of the system is greatly improved, with the bandgap changing from 1.129 eV to 0 eV. The microscopic mechanism of the interaction of gas molecules with CoO-SnSe monolayers has been revealed by energy band structure (Eg), density of states (DOS), Milligan charge analysis ( ∆Q) and electrostatic potential. Finally, the sensitivity (S) and desorption time ( τ) of the five adsorption systems were calculated to illustrate the macroscopic gas-sensitive properties of the system. This work will help to explore the application of CoO-SnSe monolayers in SF6 decomposition gas sensing detection and adsorption treatment.
作者机构：
[Hao Li; Shan Zeng; Yaqin Li; Chaoxian Liu] School of Mathematics and Computer Science, Wuhan Polytechnic University, Wuhan 430048, China;Author to whom correspondence should be addressed.;[Yong Ma] Electronic Information School, Wuhan University, Wuhan 430072, China;[Xiaorui Xiong] School of Mathematics and Computer Science, Wuhan Polytechnic University, Wuhan 430048, China<&wdkj&>Author to whom correspondence should be addressed.
通讯机构：
[Xiaorui Xiong] S;School of Mathematics and Computer Science, Wuhan Polytechnic University, Wuhan 430048, China<&wdkj&>Author to whom correspondence should be addressed.
摘要：
The immense representation power of deep learning frameworks has kept them in the spotlight in hyperspectral image (HSI) classification. Graph Convolutional Neural Networks (GCNs) can be used to compensate for the lack of spatial information in Convolutional Neural Networks (CNNs). However, most GCNs construct graph data structures based on pixel points, which requires the construction of neighborhood matrices on all data. Meanwhile, the setting of GCNs to construct similarity relations based on spatial structure is not fully applicable to HSIs. To make the network more compatible with HSIs, we propose a staged feature fusion model called SFFNet, a neural network framework connecting CNN and GCN models. The CNN performs the first stage of feature extraction, assisted by adding neighboring features and overcoming the defects of local convolution; then, the GCN performs the second stage for classification, and the graph data structure is constructed based on spectral similarity, optimizing the original connectivity relationships. In addition, the framework enables the batch training of the GCN by using the extracted spectral features as nodes, which greatly reduces the hardware requirements. The experimental results on three publicly available benchmark hyperspectral datasets show that our proposed framework outperforms other relevant deep learning models, with an overall classification accuracy of over 97%.
摘要：
Factors contributing to the quality deterioration of frozen dough encompass yeast inactivation, gluten network structure disruption, starch crystallinity increment, and ice crystal redistribution. Wheat starch, the primary constituent of dough, comprising approximately 75% of the flour mass fraction, acts a pivotal part in the properties of frozen dough and its final products. This review offers a comprehensive perspective on the mechanisms underlying frozen dough degradation, the physicochemical attributes of various wheat starch types, alterations in the structure and functional profiles of wheat starch during freezing, and correlation between changes in these properties and the decline in frozen dough quality. Significant disparities exist between A-Type and B-Type starch granules, including differences in chemical composition, crystallinity, gel formation, gelatinization, and baking properties. Consequently, the particle sizes of wheat starch may exert varying extent of impact on the degradation of frozen dough.
作者机构：
National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Wuxi 214122, China;State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, China;School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China;Analysis and Testing Center, Jiangnan University, Wuxi 214122, China;[Wangyang Shen] School of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China
通讯机构：
[Rongrong Ma] S;School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China<&wdkj&>State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, China<&wdkj&>Author to whom correspondence should be addressed.
关键词：
small-angle X-ray scattering;starch;radius of gyration
摘要：
Starch retrogradation is a complex process involving changes in the multi-scale structure. In particular, the particle order of retrograded starch is unclear. In this study, we measured the radius of gyration (Rg) and radius of particles (R) of retrograded starch using small-angle X-ray scattering. Retrograded starch included various Rg, and the values of Rg depended on the length and state of the starch chains. With time, the standard deviations of R decreased due to the increase in particle uniformity. Based on these results, a new method for assessing the degree of starch retrogradation was established from the perspective of the particle order. The accuracy of the new method was verified through differential scanning calorimetry and scanning electron microscopy. The microstructures of the samples indicated that the retrograded starch granules contained substructures (primary particles) of different sizes. This study provides a new perspective for analyzing the structure of retrograded starch.
关键词：
air decomposition pollutants;MoS2;ZnO;TiO2;gas sensor;DFT
摘要：
When partial discharges occur in air-insulated equipment, the air decomposes to produce a variety of contamination products, resulting in a reduction in the insulation performance of the insulated equipment. By monitoring the concentration of typical decomposition products (CO, NO, and NO(2)) within the insulated equipment, potential insulation faults can be diagnosed. MoS(2) has shown promising applications as a gas-sensitive semiconductor material, and doping metal oxides can improve the gas-sensitive properties of the material. Therefore, in this work, MoS(2) has been doped using the popular metal oxides (ZnO, TiO(2)) of the day, and its gas-sensitive properties to the typical decomposition products of air have been analyzed and compared using density functional theory (DFT) calculations. The stability of the doped system was investigated using molecular dynamics methods. The related adsorption mechanism was analyzed by adsorption configuration, energy band structure, density of states (DOS) analysis, total electron density (TED) analysis, and differential charge density (DCD) analysis. Finally, the practical application of related sensing performance is evaluated. The results show that the doping of metal oxide nanoparticles greatly improves the conductivity, gas sensitivity, and adsorption selectivity of MoS(2) monolayer to air decomposition products. The sensing response of ZnO-MoS(2) for CO at room temperature (25 °C) reaches 161.86 with a good recovery time (0.046 s). TiO(2)-MoS(2) sensing response to NO(2) reaches 3.5 × 10(6) at 25 °C with a good recovery time (0.108 s). This study theoretically solves the industrial challenge of recycling sensing materials and provides theoretical value for the application of resistive chemical sensors in air-insulated equipment.
作者机构：
[Liu, Yanfeng; Chen, Junsheng; Chen, Jibing; Shi, Qianyu] Wuhan Polytechnic University, No.68 Xuefunan Road Changqing garden Street Wuhan City Hubei Province, Wuhan, 430023, CHINA;[Wang, Hongze] Shanghai Jiao Tong University, Research Institute of Special Materials, School of Materials Science and Engineering, Shanghai Jiao Tong University, State Key Laboratory of Metal-based Composites, Shanghai Jiao Tong University, Shanghai, 200240, CHINA
摘要：
The application of additive manufacturing (AM) technology plays a significant role in various fields, incorporating a wide range of cutting-edge technologies such as aerospace, medical treatment, electronic information, and materials. It is currently widely adopted for medical services, national defense, and industrial manufacturing. In recent years, AM has also been extensively employed to produce bone scaffolds and implant materials. Through AM, products can be manufactured without being constrained by complex internal structures. AM is particularly advantageous in the production of macroscopically irregular and microscopically porous biomimetic bone scaffolds, with short production cycles required. In this paper, AM commonly used to produce bone scaffolds and orthopedic implants is overviewed to analyze the different materials and structures adopted for AM. The applications of antibacterial bone scaffolds and bone scaffolds in biologically relevant animal models are discussed. Also, the influence on the comprehensive performance of product mechanics, mass transfer, and biology is explored. By identifying the reasons for the limited application of existing AM in the biomedical field, the solutions are proposed. This study provides an important reference for the future development of AM in the field of orthopedic healthcare. In conclusion, various AM technologies, the requirements of bone scaffolds and the important role of AM in building bridges between biomaterials, additives, and bone tissue engineering scaffolds are described and highlighted. Nevertheless, more caution should be exercised when designing bone scaffolds and conducting in vivo trials, due to the lack of standardized processes, which prevents the accuracy of results and reduces the reliability of information.
作者机构：
[Yadong Tang; Guoliang Bai; Zhenbin Wu] State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China;School of Resources and Environmental Engineering, Wuhan University of Technology, Wuhan 430070, China;University of Chinese Academy of Sciences, Beijing 100049, China;[Huan Liao] School of Chemistry and Environmental Engineering, Wuhan Polytechnic University, Wuhan 430023, China;[Qingjun Fang; Hang Yang] State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China<&wdkj&>School of Resources and Environmental Engineering, Wuhan University of Technology, Wuhan 430070, China
通讯机构：
[Hang Yang; Yi Zhang] S;State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China<&wdkj&>School of Resources and Environmental Engineering, Wuhan University of Technology, Wuhan 430070, China<&wdkj&>State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China<&wdkj&>University of Chinese Academy of Sciences, Beijing 100049, China
关键词：
West Lake;Overlying water;Spatial variations;Seasonal differences;Sediments
摘要：
A comprehensive investigation of the physicochemical properties of overlying water and sediments in Hangzhou West Lake, China was conducted. A wide range of crucial physical parameters were analyzed, revealing significant spatial and seasonal differences. Total nitrogen (TN), total phosphorus (TP), and chemical oxygen demand (COD) concentrations in overlying water met the Class III limits (TN ≤2 mg/L, TP ≤0.2 mg/L, COD ≤20 mg/L) of the Environmental Quality Standard for Surface Waters (GB3838–2002), indicating relatively good overall water quality. However, concerns were raised regarding potential eutrophication, particularly evident during summer with explosive chlorophyll-a growth in certain lake areas like WH (266 mg/L) and XLH (239 mg/L). Lakes receiving direct diversion showed lower TN and TP versus non-diverted lakes. This research provides valuable insight into the complex nutrient dynamics and conditions of West Lake, improving understanding of lake ecosystem management and sustainable conservation strategies.
摘要：
BACKGROUND: Rosmarinic acid (RA) is an active polyphenol that is widely found in various edible herbs. This study was to explore the potential anti-allergic activities and the underling mechanisms of RA on ovalbumin (OVA)-induced intestinal allergic mice. RESULTS: Forty female BALB/c mice were randomly divided into five groups: control group, model group (OVA sensitized/challenged), RA-Low group (OVA sensitized/challenged, 30 mg/kg RA intervention), RA-Middle group (OVA sensitized/challenged, 90 mg/kg RA intervention) and RA-High group (OVA sensitized/challenged, 270 mg/kg RA intervention). RA effectively attenuated the allergic reactions, including alleviating the allergic symptoms and regulating the hypothermia of mice in the model group. Moreover, the anaphylactic mediators (OVA specific IgE (OVA-sIgE), histamine and mMCP-1) levels of OVA allergic mice were remarkably decreased after RA intervention. The q-PCR analysis showed that RA significantly inhibited the Th2 cytokines expression, while the Th1 and Treg cytokines were remarkably increased. 16S rRNA gene sequence analysis indicated that RA effectively regulated the richness and diversity of the intestinal microbiota in OVA allergic mice. In the phyla level, the relative abundance of Bacteroidetes and Firmicutes and the Firmicutes/ Bacteroidetes ratio were altered by RA intervention. In the genus level, RA showed to regulate the disturbances in the relative abundance of Muribaculaceae, Lactobacillus and Prevotella. CONCLUSION: RA exhibited potential anti-allergic activity in OVA allergic mice by regulating hypersensitive immune responses and the intestinal microbiota structure. These provide important evidence that RA can be developed into a novel functional food derived ingredient against food allergy. This article is protected by copyright. All rights reserved.
作者机构：
Hubei Key Laboratory for Processing and Transformation of Agricultural Products, Wuhan Polytechnic University, Wuhan, 430023, China;Food Safety Research Center, Key Research Institute of Humanities and Social of Hubei Province, Wuhan Polytechnic University, Wuhan, 430023, China;[Hao-Long Zeng] Department of Laboratory Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China;[Jieli Shang; Binmei Jia; Xuanpei Wang; Lin Xu; Xin Liu; Min Fang; Zhiyong Gong; Qing Yang] Hubei Key Laboratory for Processing and Transformation of Agricultural Products, Wuhan Polytechnic University, Wuhan, 430023, China<&wdkj&>Food Safety Research Center, Key Research Institute of Humanities and Social of Hubei Province, Wuhan Polytechnic University, Wuhan, 430023, China
通讯机构：
[Qing Yang] H;Hubei Key Laboratory for Processing and Transformation of Agricultural Products, Wuhan Polytechnic University, Wuhan, 430023, China<&wdkj&>Food Safety Research Center, Key Research Institute of Humanities and Social of Hubei Province, Wuhan Polytechnic University, Wuhan, 430023, China
摘要：
Ovalbumin (OVA), a major allergen in egg-induced food allergy, is widely used in the food industry. This study investigated the structural and antigenic changes of OVA after covalent conjugation with rosmarinic acid (RA). The SDS-PAGE and RA binding equivalent analysis confirmed that both the radical and alkaline methods successfully prepared the OVA-RA conjugates. LC-MS/MS assay identified 20 and 15 modified sites of RA on OVA in the radical and alkaline OVA-RA conjugates, respectively. The structural changes of OVA were indicated by fluorescence spectroscopy, Fourier transform infrared (FTIR) spectroscopy, circular dichroism (CD), and ultraviolet absorption (UV) spectrometry. Following conjugation with RA, the antioxidant capacities of OVA for scavenging DPPH• and ABTS•+ showed significant enhancement. Subsequently, the impact of RA on the antigenicity of OVA was assessed both in vitro and in vivo. The ELISA analysis revealed a notable reduction in the binding capacity of OVA to IgG and IgE after covalent conjugation with RA. Additionally, the passive cutaneous anaphylaxis (PCA) assay demonstrated lower allergenicity in radical and alkaline OVA-RA conjugates compared to native OVA. These findings collectively suggest that OVA-RA conjugates hold promise for the development of hypoallergenic food.
