期刊:
Chemical Physics Letters,2023年813:140322 ISSN:0009-2614
通讯作者:
Chen, Licai(chenlc1219@whut.edu.cn)
作者机构:
[Zhang, Tingting] Wuhan Polytech Univ, Sch Chem & Environm Engn, 68 Xuefu South Rd, Wuhan 430023, Hubei, Peoples R China.;[Li, Mingliang; Chen, Licai; Zhao, Yunliang] Wuhan Univ Technol, Sch Resources & Environm Engn, 34 Wenzhi St, Wuhan 430070, Hubei, Peoples R China.;[Bai, Haoyu] Imperial Coll London, Dept Mat, London SW7, England.;[Wang, Wei] Zhengzhou Univ, Sch Chem Engn, Zhengzhou 450001, Henan, Peoples R China.;[Zhao, Yunliang] Wuhan Clayene Technol Co Ltd, 36 Tangxunhu North Rd, Wuhan 430223, Hubei, Peoples R China.
通讯机构:
[Licai Chen; Yunliang Zhao] S;School of Resources and Environmental Engineering, Wuhan University of Technology, 34 Wenzhi Street, Wuhan, Hubei 430070, China<&wdkj&>School of Resources and Environmental Engineering, Wuhan University of Technology, 34 Wenzhi Street, Wuhan, Hubei 430070, China<&wdkj&>Wuhan Clayene Technology Co., Ltd., 36 Tangxunhu North Road, Wuhan, Hubei 430223, China
作者机构:
[Li, Xiaoqian; Zheng, Kaixin; Han, Shuangxue; He, Zhijun; Liu, Qiong; Ni, Jiazuan; Huang, Yingying] Shenzhen Univ, Coll Life Sci & Oceanog, Shenzhen Key Lab Marine Biotechnol & Ecol, Shenzhen 518055, Peoples R China.;[Han, Shuangxue] Shenzhen Univ, Coll Phys & Optoelect Engn, Shenzhen 518060, Peoples R China.;[Xiao, Peng; Hu, Xia; Xie, Qingguo] Huazhong Univ Sci & Technol, Coll Life Sci & Technol, Wuhan 430074, Peoples R China.;[Liu, Qiong; Ni, Jiazuan] Shenzhen Bay Lab, Shenzhen 518055, Peoples R China.;[Liu, Qiong; Ni, Jiazuan] Shenzhen Fundamental Res Inst, Shenzhen Hong Kong Inst Brain Sci, Shenzhen 518055, Peoples R China.
通讯机构:
[Qingguo Xie] C;[Qiong Liu] S;College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China<&wdkj&>Department of Medical Physics and Biomedical Engineering, Istituto Neurologico Mediterraneo, Neuromed IRCCS, 86077 Pozzilli, Italy<&wdkj&>Shenzhen Key Laboratory of Marine Biotechnology and Ecology, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518055, China<&wdkj&>Shenzhen Bay Laboratory, Shenzhen 518055, China<&wdkj&>Shenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research Institutions, Shenzhen 518055, China
摘要:
Abstract: The reduction of the cerebral glucose metabolism is closely related to the activation of the NOD-like receptor protein 3 (NLRP3) inflammasome in Alzheimer’s disease (AD); however, its underlying mechanism remains unclear. In this paper, 18F-flurodeoxyglucose positron emission tomography was used to trace cerebral glucose metabolism in vivo, along with Western blotting and immunofluorescence assays to examine the expression and distribution of associated proteins. Glucose and insulin tolerance tests were carried out to detect insulin resistance, and the Morris water maze was used to test the spatial learning and memory ability of the mice. The results show increased NLRP3 inflammasome activation, elevated insulin resistance, and decreased glucose metabolism in 3×Tg-AD mice. Inhibiting NLRP3 inflammasome activation using CY-09, a specific inhibitor for NLRP3, may restore cerebral glucose metabolism by increasing the expression and distribution of glucose transporters and enzymes and attenuating insulin resistance in AD mice. Moreover, CY-09 helps to improve AD pathology and relieve cognitive impairment in these mice. Although CY-09 has no significant effect on ferroptosis, it can effectively reduce fatty acid synthesis and lipid peroxidation. These findings provide new evidence for NLRP3 inflammasome as a therapeutic target for AD, suggesting that CY-09 may be a potential drug for the treatment of this disease. Keywords: Alzheimer’s disease; glucose metabolism; NLRP3 inflammasome; insulin resistance; oxidative stress; CY-09
通讯机构:
[Geng, X ] W;Wuhan Polytech Univ, Sch Civil Engn & Architecture, Wuhan 430048, Peoples R China.
关键词:
low cycle fatigue;crack growth;cracked plate;experimental study
摘要:
Abstract: The traditional study on fatigue strength for ship structures usually focuses on high cycle fatigue and ignores low cycle fatigue. However, given the recent trend towards large-scale ship development, the stress and deformation experienced by ship structures are becoming increasingly significant, leading to greater attention being paid to low cycle fatigue damage. Therefore, experimental and numerical studies on crack propagation behavior of cracked plates under low cycle fatigue loads were carried out in this paper, in order to explain the fatigue crack propagation mechanism. The effect of the stress ratio and maximum applied load on the crack propagation behavior was investigated by conducting experimental research on the cracked plate of AH32 steel. The experimental results show that an increasing maximum applied load and decreasing stress ratio will shorten the fatigue life of the cracked plate. Meanwhile, based on the finite element method, the distribution of the stress–strain field at the crack tip and the effect of crack closure were evaluated. The influencing factors such as the stress ratio and crack length were considered in numerical studies, which provided a new way to study the low cycle fatigue crack propagation behavior. Keywords: low cycle fatigue; crack growth; cracked plate; experimental study
期刊:
Journal of Materials Engineering and Performance,2023年32(1):135-143 ISSN:1059-9495
通讯作者:
Jili Liu<&wdkj&>Junsheng Yang
作者机构:
[Li, Jiang; Liu, Jili; Li, Xide; Liu, Yuzuo; Zhu, Yan] Wuhan Univ Technol, Dept Mech & Engn Struct, Wuhan 430070, Peoples R China.;[Fan, Yiquan; Yang, Junsheng; Liu, Yuzuo] Wuhan Polytech Univ, Sch Mech Engn, Wuhan 430048, Peoples R China.;[Liu, Jili] Wuhan Univ Technol, Hubei Key Lab Theory & Applicat Adv Mat Mech, Wuhan 430070, Peoples R China.;[Liu, Bing] Wuhan Second Ship Design & Res Inst, Wuhan 430064, Peoples R China.
通讯机构:
[Jili Liu] D;[Junsheng Yang] S;Department of Mechanics and Engineering Structure, Wuhan University of Technology, Wuhan, China<&wdkj&>Hubei Key Laboratory of Theory and Application of Advanced Materials Mechanics, Wuhan University of Technology, Wuhan, China<&wdkj&>School of Mechanical Engineering, Wuhan Polytechnic University, Wuhan, China
作者机构:
[Guo, Siqi; Cheng, Ningyan; Ge, Binghui; Zhang, Jialin] Anhui Univ, Inst Phys Sci & Informat Technol, Informat Mat & Intelligent Sensing Lab Anhui Prov, Key Lab Struct & Funct Regulat Hybrid Mat,Minist, Hefei 230601, Peoples R China.;[Ren, Long; Ji, Yuan] Wuhan Univ Technol, Int Sch Mat Sci & Engn, State Key Lab Adv Technol Mat Synth & Proc, Wuhan 430070, Peoples R China.;[Liu, Huating] Wuhan Polytech Univ, Sch Elect & Elect Engn, Wuhan 430023, Peoples R China.;[Yang, Ming] Beihang Univ, Sch Phys, Beijing 100191, Peoples R China.
通讯机构:
[Cheng, NY; Ge, BH ] A;[Ren, L ] W;Anhui Univ, Inst Phys Sci & Informat Technol, Informat Mat & Intelligent Sensing Lab Anhui Prov, Key Lab Struct & Funct Regulat Hybrid Mat,Minist, Hefei 230601, Peoples R China.;Wuhan Univ Technol, Int Sch Mat Sci & Engn, State Key Lab Adv Technol Mat Synth & Proc, Wuhan 430070, Peoples R China.
关键词:
carbothermal reduction;electrocatalysts;in situ;structure evolution;transmission electron microscopy
摘要:
Atomic understanding of a chemical reaction can realize the programmable design and synthesis of desired products with specific compositions and structures. Through directly monitoring the phase transition and tracking the dynamic evolution of atoms in a chemical reaction, in situ transmission electron microscopy (TEM) techniques offer the feasibility of revealing the reaction kinetics at the atomic level. Nevertheless, such investigation is quite challenging, especially for reactions involving multi-phase and complex interfaces, such as the widely adopted carbothermal reduction (CTR) reactions. Herein, in-situ TEM is applied to monitor the CTR of Co3O4 nanocubes on reduced graphene oxide nanosheets. Together with the first-principle calculation, the migration route of Co atoms during the phase transition of the CTR reaction is revealed. Meanwhile, the interfacial edge-dislocations/stress-gradient is identified as a result of the atomistic diffusion, which in turn can affect the morphology variation of the reactants. Accordingly, controllable synthesis of Co-based nanostructure with a desirable phase and structure has been achieved. This work not only provides atomic kinetic insight into CTR reactions but also offers a novel strategy for the design and synthesis of functional nanostructures for emerging energy technologies.
作者机构:
[Li, Li; Chen, Xiao-Qian; Yu, Shang-Bin; Li, Chun-Yang; Liu, Qian-Rong; Jiang, Hai-Feng] Huazhong Univ Sci & Technol, Tongji Med Coll, Dept Pathophysiol, Key Lab Neurol,Minist Educ HUST, Wuhan 430030, Peoples R China.;[Lai, Xiao-Jing] Wuhan Polytech Univ, Sch Life Sci & Technol, Wuhan 430023, Peoples R China.;[Yi, Cheng-La] Huazhong Univ Sci & Technol, Tong Ji Hosp, Tong Ji Med Coll, Dept Traumat Surg, Wuhan 430030, Peoples R China.
通讯机构:
[Cheng-La Yi; Xiao-Qian Chen] D;Department of Traumatic Surgery, Tong-ji Hospital, Tong-ji Medical College, Huazhong University of Science and Technology, Wuhan, China<&wdkj&>Department of Pathophysiology, Tongji Medical College;Key Laboratory of Neurological Diseases, The Ministry of Education (HUST), Huazhong University of Science and Technology, Wuhan, China
摘要:
The discovery of neuroglobin (Ngb), a brain- or neuron-specific member of the hemoglobin family, has revolutionized our understanding of brain oxygen metabolism. Currently, how Ngb plays such a role remains far from clear. Here, we report a novel mechanism by which Ngb might facilitate neuronal oxygenation upon hypoxia or anemia. We found that Ngb was present in, co-localized to, and co-migrated with mitochondria in the cell body and neurites of neurons. Hypoxia induced a sudden and prominent migration of Ngb towards the cytoplasmic membrane (CM) or cell surface in living neurons, and this was accompanied by the mitochondria. In vivo, hypotonic and anemic hypoxia induced a reversible Ngb migration toward the CM in cerebral cortical neurons in rat brains but did not alter the expression level of Ngb or its cytoplasm/mitochondria ratio. Knock-down of Ngb by RNA interference significantly diminished respiratory succinate dehydrogenase (SDH) and ATPase activity in neuronal N2a cells. Over-expression of Ngb enhanced SDH activity in N2a cells upon hypoxia. Mutation of Ngb at its oxygen-binding site (His(64)) significantly increased SDH activity and reduced ATPase activity in N2a cells. Taken together, Ngb was physically and functionally linked to mitochondria. In response to an insufficient oxygen supply, Ngb migrated towards the source of oxygen to facilitate neuronal oxygenation. This novel mechanism of neuronal respiration provides new insights into the understanding and treatment of neurological diseases such as stroke and Alzheimer's disease and diseases that cause hypoxia in the brain such as anemia.
关键词:
Sc modification;DED;microstructure;mechanical property;heat-affected zone
摘要:
Abstract: Aluminum alloy is an important material used in railway train structures. It is of great significance to repair aluminum alloy through directional energy deposition to reduce cost and improve the performance of the aluminum alloy. In this study, 7N01 aluminum alloy was repaired by means of laser-directed energy deposition (DED) with the powder of Sc-modified Al-Zn-Mg aluminum alloy as raw material. The microstructure and mechanical properties of the repaired specimens were studied through the metallographic microscope, scanning electron microscope, electron backscatter diffraction, universal tensile test, and Vickers hardness test in combination. The results show that the bonding interface of the repaired aluminum alloy is satisfactory, and the porosity is 2.8%. The grains in the repaired area are the columnar crystals growing vertically along the boundary of the melt pool with an obvious temperature gradient. Fine equiaxed crystals are distributed along the boundary of the melt pool, and Al3(Sc,Zr) particles play a role in grain refinement. The average grain size of the fine grain area in the repair zone next to the fusion zone is 9.1 μm, and the average grain size of the coarse grain area is 20 μm. The average tensile strength in the area of repair approaches 349 MPa, which is 91% that of the base material, and the elongation rate is 10.9%, which is 53.2% that of the base material. The hardness ranges between 122 HV and 131 HV, which is comparable to the base material. However, there is a significant decrease in the tensile strength and hardness of the base material (heat-affected zone). Keywords: Sc modification; DED; microstructure; mechanical property; heat-affected zone
通讯机构:
[Zhang, YP ] W;Wuhan Polytech Univ, Key Lab Deep Proc Major Grain & Oil, Minist Educ, Wuhan 430023, Peoples R China.
关键词:
Rice bran protein particle;Transglutaminase;Interfacial behavior;Molecular structure;Pickering foam
摘要:
The improvement of rice bran protein (RBP) foaming properties by continuous Transglutaminase (TGase) enzymatic cross-linking was studied and the related mechanism was analyzed. Under the conditions of enzyme addition of 0.5 katal/kg of protein and 3 h of enzyme crosslinking time, the foam capacity value and foam volume ratio of BPR were increased to 375% and 79.45% respectively and the liquid drainage rate of RBP samples foam system was decreased. Particle size distribution, zeta potential and surface hydrophobicity indicated that TGase treatment has changed the surface properties and aggregation state of RBP affecting the adsorption of TGase treated RBP on the interface. Meanwhile circular dichroism and intrinsic fluorescence suggested that TGase treatment made the tertiary structure of RBP aggregation more compact. The behavior of the air-water interface revealed that compared to native RBP, TGase cross-linked RBP exhibited a faster and more complex adsorption process, and a higher surface dilatational modulus. Enzyme cross-linked RBP particles mainly stabilized the interfacial membrane by Pickering mechanism, and TGase could catalyze cross-linking of RBP at the interface to enhance the elastic modulus of the interface. This study provides theoretical support for application of protein particles prepared by TGase enzymatic cross-linking in foamed foods.
通讯机构:
[Wei, N ] W;Wuhan Polytech Univ, Coll Med & Hlth Sci, Dept Rehabil Sci, Wuhan, Hubei, Peoples R China.
关键词:
Dynapenia;anthropometric measurements;cut-off values;receiver operating characteristic curve analysis;the five times sit-to-stand test
摘要:
PURPOSE: This study evaluated whether anthropometric measurements and the five times sit-to-stand test could be used to identify dynapenia. The cut-off values of accurate screening tools for identifying dynapenia were also established. MATERIALS AND METHODS: This was a cross-sectional study conducted on individuals ≥ 60 years old (N = 529). All participants underwent handgrip strength measurement, anthropometric measurements and the five times sit-to-stand test. The participants whose handgrip strength was < 28 kg for men and < 18 kg for women were considered to have dynapenia. The association between the recorded variables and dynapenia was determined using logistic regression, and cut-off values were established by performing the Receiver Operating Characteristic curve analysis. RESULTS: The prevalence of dynapenia was 35.42% in men and 25.61% in women. For males, both calf circumference (≤ 35.2 cm) and the five times sit-to-stand test (≥ 14.6 s) could be used as accurate tools for dynapenia. For females, only the five times sit-to-stand test (≥ 11.8 s) had sufficient accuracy to be used as a screening tool for dynapenia. CONCLUSIONS: The five times sit-to-stand test was an accurate screening tool for identifying dynapenia. The calf circumference could be only used as a screening tool in males.