作者机构：
National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Wuxi 214122, China;State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, China;School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China;Analysis and Testing Center, Jiangnan University, Wuxi 214122, China;[Wangyang Shen] School of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China
通讯机构：
[Rongrong Ma] S;School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China<&wdkj&>State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, China<&wdkj&>Author to whom correspondence should be addressed.
关键词：
small-angle X-ray scattering;starch;radius of gyration
摘要：
Starch retrogradation is a complex process involving changes in the multi-scale structure. In particular, the particle order of retrograded starch is unclear. In this study, we measured the radius of gyration (Rg) and radius of particles (R) of retrograded starch using small-angle X-ray scattering. Retrograded starch included various Rg, and the values of Rg depended on the length and state of the starch chains. With time, the standard deviations of R decreased due to the increase in particle uniformity. Based on these results, a new method for assessing the degree of starch retrogradation was established from the perspective of the particle order. The accuracy of the new method was verified through differential scanning calorimetry and scanning electron microscopy. The microstructures of the samples indicated that the retrograded starch granules contained substructures (primary particles) of different sizes. This study provides a new perspective for analyzing the structure of retrograded starch.
作者机构：
[Han, Qian; Qin, Zhenhua; Zhang, Wei; Wang, Pingping; Shu, Zaixi; Zhu, Lijie; Liu, Xiuying] School of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, Hubei 430028, China;[Han, Qian; Zhang, Wei; Wang, Pingping; Shu, Zaixi; Zhu, Lijie; Liu, Xiuying] Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education Wuhan, Hubei 430028, China;[Zhang, Zexin; Yang, Min; Li, Xuepeng; Bai, Xinwen] College of Food Science and Technology, Bohai University, Jinzhou, Liaoning 121013, China;[Qin, Zhenhua] School of Chemical and Environmental Engineering, Wuhan Polytechnic University, Wuhan, Hubei 430028, China
通讯机构：
[Xiuying Liu; Zhenhua Qin; Lijie Zhu] S;School of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, Hubei 430028, China<&wdkj&>Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education Wuhan, Hubei 430028, China<&wdkj&>School of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, Hubei 430028, China<&wdkj&>School of Chemical and Environmental Engineering, Wuhan Polytechnic University, Wuhan, Hubei 430028, China
关键词：
Amine vapor;Fish freshness;Fluorescent film;Ratiometric indicator;Sensing tag
摘要：
In this study, amine vapor-sensitive films with ratiometric fluorescence attributes were developed. The pH-sensitive fluorescein 8-hydroxypyrene-1,3,6-trisulfonic acid trisodium salt (HPTS) and its tetraphenylethylene derivative (TPB) were selected as ratiometric indicators and incorporated into a polyvinyl alcohol (PVA) matrix to produce HPTS/TPB-PVA films. The films responded well to amine vapors, and the interference of aromatic vapors did not substantially affect the fluorescence signals of the films. Under UV light at a wavelength of 365 nm, the fluorescence of the films changed from dark pink to light pink and finally to yellow when the freshness of the fish was visually checked during storage. In addition, the color difference values of the films showed a positive correlation with the total volatile basic nitrogen (TVB-N), ranging from 12.7 to 24.8 mg/100 g at 25 °C and 8.4 to 25.6 mg/100 g at 4 °C, respectively. This indicates that fluorescent films have good potential for quantifying fish freshness in the near future when connected to an automatic data processing system based on color differences.
期刊：
FRONTIERS IN NUTRITION,2024年11:1334809 ISSN：2296-861X
作者机构：
College of Food Science and Engineering, Wuhan Polytechnic University, China;Key Laboratory for Deep Processing of Major Grain and Oil (Wuhan Polytechnic University), Ministry of Education, China;School of Liquor and Food Engineering, Guizhou University, China
摘要：
The planting area of high-quality indica rice varieties has been growing rapidly in China. However, the storage characteristics of these varieties remains unclear. In this research, different moisture contents (13.5, 14.5, and 15.5%) of high-quality rice (variety Xiadao No.1) were stored at different temperatures (15, 20, 25, and 30°C) for 360 d, and then evaluated for lipid metabolism, redox enzyme activities, fatty acid composition, and sensory attributes. With the prolongation of storage, rice displayed an upward trend in fatty acid value, malondialdehyde content, and cooked rice hardness and a downward trend in contents of total fat and non-starch lipid, peroxidase and catalase activities, and sensory score of cooked rice. The change trends of these quality parameters were aggravated by elevating storage temperature and moisture content. Linoleic acid content of rice generally decreased with prolonged storage. After 300 d of storage, rice with initial moisture content of 13.5% at 30°C showed a fatty acid value of higher than 30 mg KOH/100 g, while rice of other two initial moisture contents reached similar level at 25°C. After the whole storage period, only rice with initial moisture contents of 13.5 and 14.5% stored at 15°C had a sensory score of higher than 60. These results suggested that the aging process of high-quality rice can be inhibited by decreasing the storage temperature and initial moisture content. These results can provide reference for grain storage enterprises to select proper storage condition to store high-quality rice.
摘要：
Ochratoxin A (OTA) poses a great threat to human health, and its rapid detection has drawn wide concerns in agri-food product risk assessment. Herein, on the basis of the aptamer-enhanced peroxidase-mimetic property of two-dimensional few-layer tungsten diselenide (WSe2) nanosheets, a simple label-free colorimetric aptasensor is presented for OTA detection. The enzymatic mimic property of the WSe2 nanosheets could be enhanced through aptamer adsorption because of the enhanced affinity of WSe2 nanosheets to 3,3 ',5,5 '-tetramethylbenzidine. A good linear relationship was obtained in the range from 0.5 to 50 ng/mL, and the limit of detection for OTA was 0.16 ng/mL. The performance of this strategy was comparable to previous strategies for OTA determination. Further, this colorimetric aptasensor displayed satisfying specificity and stability in a complex food matrix including both red wine and wheat flour samples. Hence, the results of this study suggest potential applications of the aptasensor to detect mycotoxins in different agri-food products.
期刊：
Ecotoxicology and Environmental Safety,2024年273:116179 ISSN：0147-6513
作者机构：
[Ding, Jiuyang; Sun, Baofei] Key Laboratory of Human Brain bank for Functions and Diseases of Department of Education of Guizhou Province, Guizhou Medical University,Guiyang 550025,China;[Ding, Jiuyang] Key Laboratory of Endemic and Ethnic Diseases, Ministry of Education, Guizhou Medical University, Guiyang 550004, China;[Ding, Jiuyang] School of Forensic Medicine, Guizhou Medical University,Guiyang 550004,China;[Zheng, Juan; Gao, Yingdong] Department of Reproductive Medicine, Taian Maternity and Child Health Hospital, Taian 271000, China;[Liu, Changyou] Department of Pediatrics, Taian Maternity and Child Health Hospital, Taian 271000, China
关键词：
Blood brain barrier;Chromium;Hypothalamus
摘要：
It has been shown that exposure to hexavalent Chromium, Cr (Ⅵ), via nasal cavity can have neurotoxicological effects and induces behavioral impairment due to the fact that blood brain barrier (BBB) does not cover olfactory bulb. But whether Cr (Ⅵ) can cross the BBB and have a toxicological effects in central nervous system (CNS) remains unclear. Therefore, we investigated the effects of Cr (Ⅵ) on mice treated with different concentrations and exposure time (14 days and 28 days) of Cr (Ⅵ) via intraperitoneal injection. Results revealed that Cr accumulated in hypothalamus (HY) in a timely dependent manner. Much more severer neuropathologies was observed in the group of mice exposed to Cr (Ⅵ) for 28 days than that for 14 days. Gliosis, neuronal morphological abnormalities, synaptic degeneration, BBB disruption and neuronal number loss were observed in HY. In terms of mechanism, the Nrf2 related antioxidant stress signaling dysfunction and activated NF-κB related inflammatory pathway were observed in HY of Cr (Ⅵ) intoxication mice. And these neuropathologies and signaling defects appeared in a timely dependent manner. Taking together, we proved that Cr (Ⅵ) can enter HY due to weaker BBB in HY and HY is the most vulnerable CNS region to Cr (Ⅵ) exposure. The concentration of Cr in HY increased along with time. The accumulated Cr in HY can cause BBB disruption, neuronal morphological abnormalities, synaptic degeneration and gliosis through Nrf2 and NF-κB signaling pathway. This finding improves our understanding of the neurological dysfunctions observed in individuals who have occupational exposure to Cr (Ⅵ), and provided potential therapeutic targets to treat neurotoxicological pathologies induced by Cr (Ⅵ).
关键词：
Air-/oil–water interfaces;Co-extraction;Emulsion/foam;Natural nanoparticle;Plant protein
摘要：
Enrichment of plant proteins with functionality is of great importance for expanding their application in food formulations. This study proposed an innovation to co-enrich soy protein and flaxseed protein to act as efficient interfacial stabilizers for generating foams and emulsions. The structure, interfacial properties, and functionalities of the soy protein-flaxseed protein natural nanoparticles (SFNPs) obtained by alkali extraction-isoelectric precipitation (AE) and salt extraction-dialysis (SE) methods were investigated. Overall, the foamability of AE-SFNPs (194.67%) was 1.45-fold that of SE-SFNPs, due to their more flexible structure, smaller particle size, and suitable surface wettability, promoting diffusion and adsorption at the air-water interface. AE-SFNPs showed higher emulsion stability (140.89min), probably because the adsorbed AE-SFNPs with smaller size displayed soft particle-like properties and stronger interfacial flexibility, and therefore could densely and evenly arrange at the interface, facilitating the formation of a stiff and solid-like interfacial layer, beneficial for more stable emulsion formation. The findings may innovatively expand the applications of SFNPs as food ingredients.
摘要：
Natural polysaccharides extracted from plants have received increasing attention due to their rich bioactivity. In our study, peach gum polysaccharides (PGPs) were extracted by water extraction-alcohol precipitation method. PGPs are typical pyranose polysaccharides with a mean molecular weight of 3.68 x 106 g/mol. The antioxidant activity and hepatoprotective capacity of PGPs were studied. In vitro, assays showed that PGPs scavenged DPPH, OH, and O2- in a dose-dependent manner. PGPs exhibited antioxidative properties against alcohol-induced HL7702 cells, as evidenced by the normalization of MDA, SOD, ROS, and GSH levels. To further elucidate the hepatoprotective mechanism of PGPs, we carried out in vivo experiments in male mice. PGPs exerted hepatoprotective effects in alcohol liver disease (ALD) mice by exerting antioxidant effects, decreasing the inflammatory response and modulating lipid metabolism. In addition, metabolomic analysis indicated that PGPs mainly regulate D-glutamine and D-glutamate metabolism, alanine, aspartate and glutamate metabolism, and arginine biosynthesis to promote hepatic metabolism and maintain body functions. Overall, this study revealed that the hepatoprotective mechanism of PGPs against ALD might be associated with the regulation of oxidative stress and lipid metabolism.
摘要：
In this study, resveratrol-loaded nano-emulsions were added to yogurts, improving the physicochemical properties and functional factors and realizing the development of nutrient-fortified yogurt. Yogurts added with free resveratrol (Y-R), resveratrol-loaded emulsions stabilized by sodium caseinate (Y-NN), decaglycerol monooleate (Y-DN), and sodium caseinate-decaglycerol monooleate (Y-DND) were evaluated for their physicochemical properties, including pH, titratable acidity, syneresis, and textural parameters, with 5-day intervals for 15-day storage. The resveratrol retention rate was analyzed in the Y-R, Y-NN, Y-DN, and Y-NDN groups during 15 days of storage. The dynamic bioaccessibility of resveratrol and the NMR-based nutritional profile of yogurt in the Y-R, Y-NN, Y-DN, and the Y-NDN group were investigated after in vitro digestion. The results demonstrated that the addition of resveratrol emulsion decreased the hardness of yogurt while evaluating its titratable acidity and water-holding capacity, which were characterized by high stability. The stability of resveratrol added in the form of an emulsion was significantly higher than that of the free form. Compared with the other groups, the yogurt formulated with sodium caseinate/decaglycerol monooleate (NaCas/DGMO) emulsion showed the highest resveratrol retention rate, about 70%. In vitro digestion showed that encapsulation effectively and persistently improved the dynamic bioaccessibility of resveratrol. Additionally, NMR-based nutritional profile analysis before and after in vitro digestion demonstrated that resveratrol emulsion nutritional fortification promoted the release of nutrients, improving the nutritional value of yogurt. These findings offered theoretical guidance and technical support for the use of resveratrol nano-emulsions in yogurt.
通讯机构：
[Dai, H ] W;Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Peoples R China.
关键词：
Curcumin;Ratiometric sensor;Electrochemical sensor;Grain products;Heavy metal ions
摘要：
The conventional electrochemical detection in food samples often suffers from low stability and poor reproducibility. The construction of ratiometric sensor is regarded as an effective means to overcome the issues. Herein, poly-curcumin (p-CCM)-Multi walled carbon nanotubes (MWCNTs)-layered double hydroxides (LDHs) was constructed as a novel rationmetric sensing material for detection of Cd2+ and Pb2+ in grain samples. Under the synergistic effect of MWCNTs and the adsorption capacity of LDHs, the p-CCM/MWCNTs/LDHs can significantly enhance the electrochemical responses of heavy metals. In the differential pulse voltammetry test, the ratio of oxidation peak currents between heavy metal ions and curcumin was directly proportional to the concentration of heavy metal ions. This has formed the basis of a new ratiometric electrochemical sensor for the detection of the levels of Cd2+ and Pb2+ in grain products. Under optimal conditions, the limits of detection for Cd2+ and Pb2+ were found to be 0.61 mu g mL(-1) and 0.74 mu g mL(-1), respectively. It is worth mentioning that the method was additionally utilized to detect Cd2+ and Pb2+ in rice and flour samples. The satisfactory recovery outcomes in both samples demonstrated minimal interference of the food matrix with the obtained ratiometric sensor. In this work, a CCM-based ratiometric electrochemical sensor was presented, which could potentially replace the widely used ferrocene-based ratiometric electrochemical sensor, thereby enhancing the exploration of electrode materials for ratiometric electrochemical sensors.
关键词：
Curcumin;emulsion;green tea polysaccharide conjugates;soybean protein isolate
摘要：
Tea processing often generates significant waste, resulting in resource inefficiency. However, extracting green tea polysaccharide conjugate (gTPC) from this waste has shown promising bioactivity. Previous studies have confirmed the emulsifying properties of gTPC. Yet, its use as an emulsifier is limited due to its requirement for high surface activity. The findings revealed that a 0.40 wt% soy protein isolate (SPI) and 0.50 wt% gTPC conjugate required 300 degrees C for complete decomposition, demonstrating the highest stability among other ratios. The Pickering emulsion, using a 0.30 wt% emulsifier ratio, exhibited exceptional stability. Moreover, it showed remarkable zeta potential and particle size stability under stressful conditions (temperature, pH and metal ions). Additionally, it effectively protected curcumin from adverse pH, light and temperature effects, with a retention rate of over 74.00%. These results highlight the gTPC/SPI combination as an effective stabiliser, offering new prospects for gTPC development and utilisation. gTPC and SPI complexes (gTPC/SPI) were prepared and characterised. gTPC/SPI had stronger emulsifying ability than that of the two alone. In emulsions, gTPC/SPI content was low (0.30 wt%) and oil content was high (60 wt%). gTPC/SPI emulsions carried curcumin and protected it in stressed environment. image
摘要：
In the realm of practical problem-solving, multi-objective optimisation problems with redundant variables and indefinite objective functions (MOPRVIF) are becoming increasingly prevalent. MOPRVIF involve determining the optimal decision variables that optimise multiple objectives, leveraging the relational data of a set of variables and multiple objectives. For these problems, this paper focuses on the following two issues: one is the demand for a unified computational model to solve this problem; the other is how to improve the algorithm's deep intelligent search capability. In this regard, this paper designs a dual data-driven multi-objective optimisation method. The method used consisted of four parts: elimination of redundant variables (ERV), objective function construction (OFC), selection evolution operator (SEO), and multi-objective evolutionary algorithm (MOEA). MOEA was the main focus of the method. ERV is data preparation and variable selection according to multiple objectives. OFC involves constructing the relationship model between variables and objectives, and a high-accuracy model is important for guaranteeing reliable results. Furthermore, SEO can adjust the evolution operator during a deep search. This is an important guarantee for deep, intelligent search. MOEA combined OFC and SEO to form the final solution algorithm-Dual Data Driven Multi-Objective Evolutionary Algorithm (DDMOEA). DDMOEA was explored using two different disciplinary problems of drug compound optimisation and wild blueberry cultivation and benchmarks were selected. The first two problem domains are distinct. The first problem is more complex than the second; however, both encompass redundant variables and indefinite objective functions. Benchmarks are utilised independently to gauge the profound intelligent search capability. The experiments affirm that the dual data -driven optimization approach proposed in this paper is effective, practical, and scalable.
作者机构：
[Fuwei Pi; Yue Zou; Shuo Duan; Xin Liu; Zaixi Shu; Xiaodan Liu; Jiahua Wang; Zhenhong Yuan; Huilin Hu] College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, 430023, China;[Shuo Duan; Xin Liu; Zaixi Shu; Xiaodan Liu; Jiahua Wang; Huilin Hu] Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, Wuhan Polytechnic University, Wuhan, 430023, China;[Qi Zhang] School of Grain Science and Technology, Jiangsu University of Science and Technology, Zhenjiang, 212004, China;[Yingli Wang] College of Engineering, Huazhong Agricultural University, Wuhan, 430070, China;[Fuwei Pi] School of Food Science, Jiangnan University, Wuxi, 214122, China
通讯机构：
[Huang Dai] C;College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, China<&wdkj&>Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, Wuhan Polytechnic University, Wuhan, China
摘要：
Aratiometric assay was designed to improve the sensitivity and reliability of electrochemical immunosensors for deoxynivalenol (DON) detection. The indicator signal caused by the Fe-based metal-organic framework nanocomposites loaded with gold nanoparticles and the internal reference signal from the [Fe(CN)(6)](3-/4-) in the electrolyte came together at the immunosensor. When immunoreactivity occurred, the indicator signals decreased as the concentration of DON increased, while the internal reference signals increased slightly. The ratio of the indicator signal to the internal reference signal was available for reproducible and sensitive monitoring of DON. The prepared immunosensor showed excellent performance in the range from 0.5 to 5000 pg mL(-1), and the detection limit was 0.0166 pg mL(-1). The immunosensor achieved satisfactory detection toward DON in spiked and actual samples and has a promising application in the control of DON in grain products.
摘要：
To improve the emulsifying property of sodium caseinate (NaCas) as stabilizer of oil-in-water emulsions and encapsulation of bioactive compounds, three hexaglycerol mono-fatty acid esters were chosen as small molecular weight surfactants to complex with NaCas. Hexaglycerol monooleate (HGMO) was found to be the optimal surfactant and the optimal mass ratio was 1:1, through characterization of particle size, Zeta-potential, and turbidity. Fluorescence and FTIR spectra indicated that the hydrophobic interaction and hydrogen bond provided driving forces to the formation of stable complex. The complexation of HGMO to NaCas increased the surface hydrophobicity and decreased surface tension compared with NaCas, and strengthened the EAI and ESI. The NaCas-HGMO complex had good stabilization on rice bran oil-in-water emulsions, in a wide pH and ionic strength, and the forwarding Cur encapsulation in O/W emulsions dramatically reduced the degradation during storage at 4celcius. Therefore, the present NaCas-HGMO complex might be employed as an effective emulsifier to stable O/W emulsions that load lipophilic bioactives in functional foods or beverages.
摘要：
The application of rapeseed protein in human foods is limited by residual antinutritive components and poor sensory quality. The effects of five extraction protocols on rapeseed protein yield, sensory, functional and nutritional properties were systematically evaluated in this study. In particular, the potential of weakly acidic salt (pH 6.5, 150mmol·L(-1) MgCl(2)) extraction as a mild method for recovering edible rapeseed protein was investigated compared with conventional alkali extraction. All salt-extracted proteins showed above 40% extraction yield and low antinutritional factor contents. They also had ideal amino acid patterns and better in vitro gastroduodenal digestibility than alkaline-extracted proteins. Additionally, the lighter color and odor, as well as better solubility, emulsion activity, foaming property, and water/oil holding capacity were found in weakly acidic salt extraction-ultrafiltered proteins. These findings suggest that weakly acidic salt extraction-ultrafiltration could be used for obtaining edible rapeseed protein, while extraction yield should be improved for scale application.
