作者机构：
[Shu H.; Zhou K.; Lyu X.; He Z.] College of Mathematics and Computer Science, Wuhan Polytechnic University, Wuhan, 430023, China;[Chen X.] School of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, 430023, China
通讯机构：
[Kang Zhou] C;College of Mathematics and Computer Science, Wuhan Polytechnic University, Wuhan, China
关键词：
Evolutionary Algorithm;Tissue P systerm;Tri-objective VRPTW
作者机构：
[He, Yi; Wang, Yuchan; Gao, Chao; Long, Pengcheng] Wuhan Polytech Univ, Natl R&D Ctr Se rich Agr Prod Proc, Hubei Engn Res Ctr Deep Proc Green Serich Agr Prod, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.;[He, Yi; Zhu, Lisha; Hu, Zhuoyu] Wuhan Polytech Univ, Sch Food Sci & Engn, Key Lab Deep Proc Major Grain & Oil, Hubei Key Lab Proc & Transformat Agr Prod,Minist E, Wuhan 430023, Peoples R China.;[Wang, Liling] Tarim Univ, Coll Food Sci & Engn, Alar 843300, Peoples R China.;[Shao, Yanchun] Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Peoples R China.;[Wang, Bin] Shihezi Univ, Sch Food Sci & Technol, Shihezi 832003, Peoples R China.
通讯机构：
[He, Y ] ;Wuhan Polytech Univ, Natl R&D Ctr Se rich Agr Prod Proc, Hubei Engn Res Ctr Deep Proc Green Serich Agr Prod, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.
摘要：
Monascus spp., a fungus of medicine food homology, produces various beneficial secondary metabolites such as monacolin K (MK) during fermentation. In this study, the mixed fermentation of Monascus with rice, Pueraria, and yam has been systematically investigated. Single-factor and response surface methodology (RSM) were carried out to maximize MK content, and the optimized fermentation results showed that the content of MK reached 1.40 +/- 0.04 mg/g. The optimal fermentation process determined the ratio of 8: 1: 1 for rice, Pueraria, and yam, respectively, the inoculation amount was 106 spores/mL, fermentation time was 15 days. Further analysis of the content of active substance in the fermentation process revealed that the beta-glycosidase activity increased, the amylase enzyme activity decreased, the dioscin content decreased by 19.5%, and the diosgenin content increased by 175%, suggesting that there was a dynamic equilibrium relationship among the changes of active substances. This study aims at provide a basis and reference for the research of Monascus fermented foods by medicine food homology raw materials.
摘要：
In this study, for the first time, a novel separation and preconcentration method of direct immersion dual-drop microextraction (DIDDME) was proposed for the species of inorganic chromium (Cr(III) and Cr(VI)) followed by graphite furnace atomic absorption spectrometry detection. The methodology is based on that two organic drops hold on the needle tips of microsyringes were concurrently immersed in a stirred sample solution. Each drop contains a chelating reagent, which can react with a specific species under the same pH value. Therefore, Cr(III) and Cr(VI) can be selectively extracted into different drops. This procedure did not require tedious and complicated pre-oxidation/pre-reduction and centrifugation/filtration operations, which may lead to the risk of sample contamination and analysis errors. Main parameters influencing separation, preconcentration and identification of the target species were investigated. An enrichment factor of 400-fold was obtained for Cr(III) and Cr(VI). Under the optimized conditions, detection limits for this method were 1.1ngL(-1) and 1.4ngL(-1) for Cr(III) and Cr(VI) with relative standard deviations of 5.1 and 6.3%, respectively. This procedure was applied for the separation, preconcentration and determination of Cr(III) and Cr(VI) in environmental water samples and certified reference materials with satisfactory results. Recoveries of spiked experiments ranged from 86.0 to 112%.
作者机构：
[Jia, Jilai; Zhou, Jiaojiao; Cai, Jie; Zhou, JJ; Lv, Xuqin; Xie, Fang] Wuhan Polytech Univ, Sch Modern Industryfor SeleniumScience & Engn, NationalR&D CenterforSe richAgriculturalProductsPr, Wuhan 430023, Peoples R China.;[Cai, Jie; Lv, Xuqin] Wuhan Polytech Univ, Hubei Key Lab Proc & Transformat Agr Prod, Key Lab Deep Proc Major Grain & Oil, Minist Educ, Wuhan 430023, Peoples R China.;[Du, Xiaoping] Minist Agr & Rural Affairs, Key Lab Se Enriched Prod Dev & Qual Control, Ankang R&D Ctr Se Enriched Prod, Ankang 725000, Shaanxi, Peoples R China.
通讯机构：
[Cai, J ; Zhou, JJ] W;Wuhan Polytech Univ, Sch Modern Industryfor SeleniumScience & Engn, NationalR&D CenterforSe richAgriculturalProductsPr, Wuhan 430023, Peoples R China.;Wuhan Polytech Univ, Hubei Key Lab Proc & Transformat Agr Prod, Key Lab Deep Proc Major Grain & Oil, Minist Educ, Wuhan 430023, Peoples R China.
关键词：
mercury ion detection;colorimetric biosensor;DNA;graphene oxide;platinum nanoparticles
摘要：
Mercury ions (Hg2+) have persistent bioaccumulation and high toxicity, which can severely damage the ecological system and human health. Therefore, sensitive methods are required for Hg2+ analysis. Herein, a DNA-mediated growth strategy was developed for the synthesis of platinum nanoparticles (PtNPs) on the surface of graphene oxide (GO). The resulting GO-DNA-PtNPs displayed excellent peroxidase-mimicking activity in a sequence-dependent manner. The peroxidase-mimicking activity could be significantly inhibited by Hg2+ because of the specific trapping of Hg2+ by thymine bases (T–Hg2+–T) and in situ reduction of Hg2+ by PtNPs on the GO surface. Under optimal conditions, there was a good linear range from 1 pM to 1 μM with a detection limit of 2.2 pM for Hg2+ analysis. This sensor demonstrated excellent analytical performance for assessing Hg2+ in complex lake waters. This work provides an effective strategy for the preparation of PtNP-based nanozymes and broadens the applications of PtNPs for biosensing.
摘要：
This study evaluated the changes in phenolic and reactive oxygen species metabolism, and microbial diversity in fresh-cut lotus roots following ethanol treatment to elucidate the potential mechanisms of ethanol in controlling microbial growth and browning. Activity suppression and gene expression downregulation of phenylalanine ammonia-lyase, polyphenol oxidase, and peroxidase took place with ethanol treatment, resulting in the decreased accumulation of total phenols and soluble quinones. The total phenol and soluble quinone contents of ethanol-treated slices were 12.63% and 30.22% lower than those in the control group, respectively, after 12 d of storage. Ethanol treatment changed the level of eleven individual phenols, two of which were associated with antioxidant ability. Besides, the ethanol-treated slices exhibited the reduced production of reactive oxygen species, increased activities of superoxide dismutase and catalase, improved ascorbic acid contents and 1,1-Diphenyl-2-picrylhydrazyl scavenging rate, and the upregulated transcriptional levels of NnGR and NnGST. Moreover, the integrated transcriptomic and metabolomic analyses show the biosynthesis of syringin (an anti-oxidant in various medicinal plants) might be stimulated by ethanol treatment. Ethanol treatment inhibited the abundance of Pantoea, Pseudomonas, Tolumonas and Stenotrophomonas. These findings suggested ethanol treat-ment is a simple and potential technology for preserving the quality and antioxidant ability of fresh-cut products.
通讯机构：
[Wu, Y ; Wang, XD; Fu, Y] W;Wuhan Polytech Univ, Coll Food Sci & Engn, Xuefu South Rd 68, Wuhan 430023, Peoples R China.
关键词：
Frozen raw noodle;Frozen storage quality improvement;Modified starch;Texture
摘要：
The impacts of both structural variations induced by low temperature and physiochemical changes induced by modified starch on the qualities of frozen raw noodles (FRNs) were investigated during long-term freezing storage. The addition of modified starch was a potentially effective method to delay the loss of FRNs qualities during storage. In this study, hydroxypropyl starch (HPS) and hydroxypropyl distarch phosphate (HPDSP) were added to improve the cooking and textural characteristics of FRNs. The cooking loss rate of FRNs with the addition of 12%HPS was consistent with that of the control (4.39 % and 4.37 %, respectively), while after 8 weeks of storage showed the significant decrease effect (5.01 % and 5.78 %, respectively). In addition, adding HPS or HPDSP could change the colour and lustre of FRNs to that preferred by consumers. When 6 % HPS or HPDSP were added, the FRNs showed the lowest of freezable water content during storage. The test results of FTIR showed the secondary structure of FRNs was maintained with the introduction of HPS or HPDSP during refrigeration, and the microstructure was improved during the frozen storage period. Consequently, the results provided a theoretical basis and new insight for the production and transportation of FRNs.
摘要：
Tribolium castaneum (Herbst) is a worldwide grain storage pest controlled by chemical control methods of phosphine fumigation, which results in many hazards, damages human health, makes pests resistant to pesticides, and pollutes the environment. In recent years, the popularity of botanical insecticides has continued to rise, and plant essential oils (EO) are considered potential alternatives for developing insecticides. In the current study, we selected the Chamaecyparis obtusa EO to determine its insecticidal effects and component analysis on T. castaneum. Through gas chromatography-ion mobility spectrometry (GC-IMS) technology, cedrol was the most obvious compound in the signal peak of the volatile components detected in the C. obtusa EO. The results of the bioassay showed that the C. obtusa EO had certain contact activity against T. castaneum, and the LD(50) was 52.54μg/adult. At three concentrations (0.41,1.62, 2.83 uL/cm(2)), the repellent rates of C. obtusa EO against T. castaneum were all above 80% at 15, 30, 60, and 120min, respectively, indicating that the repellent effect was strong. Meanwhile, the C. obtusa EO exhibited fumigant toxicity against T. castaneum with LC(50) values of 7.09μg/L air. In addition, C. obtusa EO significantly increased the activity of AChE, CarE, POD, CAT, T-SOD, and chitinase in T. castaneum. Finally, the mechanism of C. obtusa EO on T. castaneum adults was explored based on transcriptome sequencing. We found that the DEGs focused on the chitin metabolic process and some aging genes in T. castaneum. Therefore, C. obtusa EO could be used as potential eco-friendly candidates for stored grain pest management.
摘要：
Photothermal therapy (PTT) is considered to be one of the promising methods to combat pathogenic bacteria. However, traditional PTT is prone to generate undesired temperature increase to surrounding normal tissues, which limits the application of PTT. Herein, an acid-responsive PTT system (Au nanoparticles system: AuNPs-S) was constructed based on the photothermal feature of spherical gold nanoparticles (AuNPs) and the low pH of the bacterial infected site. AuNPs-S is composed of two kinds of AuNPs: AuNPs modified with Asp-Asp-Asp-Asp-Asp-Cys (peptide A) were denoted as AuNPs-A; AuNPs modified with 2,3-dimethylmaleic anhydride (DA) grafted Lys-Gly-Gly-Lys-Gly-Gly-Lys-Cys (peptide B) were denoted as AuNPs-B/DA. AuNPs-B/DA with an acid-responsive moiety showed a charge-convertible feature. The negatively charged AuNPs-B/DA became positively charged AuNPs-B at low pH, aggregating with the negatively charged AuNPs-A via an electrostatic interaction, reaching the threshold to the interparticle plasmonic coupling effect among AuNPs, thereby killing bacteria precisely under the irradiation of near-infrared (NIR) light through the elevated temperature at the targeted area. This acid-responsive PTT strategy supplies an excellent mode for combating bacterial infections with no vital damage to normal tissues.
