通讯机构：
[Liang Luo] N;National Engineering Research Center for Nanomedicine, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China<&wdkj&>Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China<&wdkj&>Hubei Key Laboratory of Bioinorganic Chemistry and Materia Medica, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan, China
摘要：
Objectives: Human infection caused by an uncommon Salmonella enterica subsp. diarizonae (hereafter S. diarizonae) is rising. However, knowledge concerning S. diarizonae is still limited. This study aimed to investigate the genomic features of S. diarizonae S499 isolated from a child patient with gastroenteritis symptom in China. Methods: The antimicrobial susceptibility of S. diarizonae S499 was determined by microdilution broth assay. Whole genome was sequenced using Illumina HiSeq X-10 and PacBio RS II platforms and was de novo assembled using Unicycler and SPAdes. Conjugation experiment was performed by a broth mating method. Results: S. diarizonae S499 was a multi-drug resistant (MDR) isolate and showed resistance to all cephalosporin drugs tested. Six plasmids (pS0499A, pS0499B, pS0499C, pS0499D, pS0499E and pS0499F) were identified. A rare gene cassette IS26-bla(CTX-M-55)-wbuc-Delta bla(TEM-1) -IS26-intI1 was repeatedly inserted into pS0499A three times in one locus and reversely inserted into plasmid pS0499D. That enhanced cephalosporin resistance. To the best of our knowledge, this finding has not been reported previously. Both pS0499A and pS0499B contained multiple resistance genes and could transfer to recipient strain E. coli EC600. Conclusion: This article reported the genome features of S. diarizonae S499, which contained four resistant plasmids including a novel plasmid pS0499A with a novel gene cassette rearrangement. These data could contribute to a better understanding of the antimicrobial resistance mechanisms and transmission dynamics of S. diarizonae. (c) 2022 The Author(s). Published by Elsevier Ltd on behalf of International Society for Antimicrobial Chemotherapy. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
作者机构：
[Xiaojie Lan; Qi Wang; Tingjue Wu; Na Li; Hongxun Wang; Ziqiang Zheng [email protected]] College of Life Science and Technology, Wuhan Polytechnic University, Wuhan, China
通讯机构：
[Ziqiang Zheng [email protected]] C;College of Life Science and Technology, Wuhan Polytechnic University, Wuhan, China
期刊：
International Journal of Food Properties,2022年25(1):1203-1214 ISSN：1094-2912
通讯作者：
Shoulei Yan
作者机构：
[Li, Jie; Yan, Shoulei; Liu, Yanzhao; Wang, Jie] Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Peoples R China.;[Li, Jie; Yan, Shoulei] Yangtze River Econ Belt Engn Res Ctr Green Dev Bu, Minist Educ, Wuhan, Peoples R China.;[Li, Jie; Yan, Shoulei] Hubei Aquat Vegetable Preservat Proc Engn Technol, Wuhan, Peoples R China.;[Diao, Ying] Wuhan Polytech Univ, Coll Life Sci & Technol, Wuhan, Peoples R China.;[Hu, Zhongli] Wuhan Univ, Coll Life Sci, Hubei Lotus Rhizome Engn Technol Res Ctr, Wuhan, Peoples R China.
通讯机构：
[Shoulei Yan] C;College of Food Science and Technology, Huazhong Agricultural University, Wuhan, China<&wdkj&>Yangtze River Economic Belt Engineering Research Center for Green Development of Bulk Aquatic Bioproducts Industry of Ministry of Education, Wuhan, China<&wdkj&>Hubei Aquatic Vegetable Preservation Processing Engineering Technology Research Center, Wuhan, China
关键词：
Lotus rhizome starch;Adulteration;Ferrous sulfate;polyphenol;Liquid chromatography mass spectrometry;Colorimetric card
摘要：
Lotus rhizome starch (LRS) is an important traditional processed product of lotus rhizome in China due to its good taste and high nutritional value. However, adulterated lotus rhizome starches are prevalent on the market. In this study, a novel method for the rapid detection of adulteration in LRS based on color reaction between ferrous sulfate (FS) and (-)-gallocatechin (GC) in LRS was studied. The polyphenols in LRS were analyzed by liquid chromatography-mass spectrometry (LC-MS/MS) as GC and (+)-catechin, and GC could show a bluish color with FS solution. LRS showed a bluish color when FS solution (0.01 mol/L) was used as the chromogenic agent, which was not observed for other ten kinds of starches tested in this work. There was an obvious color gradient when FS solution was used to react with adulterated LRS at different proportions, and the change in blue color was obvious when maize starch or cassava starch was adulterated in LRS at the ratio of 6:4. Then, a semi-quantitative method was used to make a color card according to the color gradient, which was then successfully used to detect the adulteration of four kinds of commercial LRS. The results indicate that the proposed method may facilitate the simple, effective and efficient detection of adulteration in LRS.
摘要：
Pseudonocardia species are emerging as important microorganisms of global concern with unique and increasingly significant ecological roles and represent a prominent source of bioactive natural products, but genetic engineering of these organisms for biotechnological applications is greatly hindered due to the limitation of efficient genetic manipulation tools. In this regard, we report here the establishment of an efficient genetic manipulation system for a newly isolated strain, Pseudonocardia alni Shahu, based on plasmid conjugal transfer from Escherichia coli to Pseudonocardia. Conjugants were yielded upon determining the optimal ratio between the donor and recipient cells, and designed genome modifications were efficiently accomplished, including exogenous gene integration based on an integrative plasmid and chromosomal stretch removal by homologous recombination using a suicidal non-replicating vector. Collectively, this work has made the P. alni Shahu accessible for genetic engineering, and provided an important reference for developing genetic manipulation methods in other rare actinomycetes.
摘要：
Cardiovascular complications of patients with type 2 diabetes mellitus (T2DM) threaten the health and life of numerous individuals. Recently, growth factor receptor-binding protein 10 (GRB10) was found to play a pivotal role in vascular complications of T2DM, which participates in the regulation of lipid metabolism of T2DM patients. The genetic variation of GRB10 rs1800504 is closely related to the risk of coronary heart disease in patients with T2DM. The development of GRB10 as a key mediator in the association of lipid metabolism with cardiovascular complications in T2DM is detailed in and may provide new potential concerns for the study of cardiovascular complications in T2DM patients.
