作者机构：
[Jingcheng Dai; Jiangke Yang] School of Life Science and Technology, Wuhan Polytechnic University, Wuhan, 430023, China;[Dingxin Wen; Yong Yang] State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Science, Hubei University, Wuhan, 430062, China;[Hao Li; Jingpeng Yang; Xiongfei Rao] Tobacco Research Institute of Hubei Province, Wuhan , Hubei, 430030, China;[Jiangke Yang] Pilot Base of Food Microbial Resources Utilization of Hubei Province, College of Life Science and Technology, Wuhan Polytechnic University, Wuhan, 430024, China;[Chunlei Yang] Tobacco Research Institute of Hubei Province, Wuhan , Hubei, 430030, China. ycl193737@163.com
通讯机构：
[Chunlei Yang; Jun Yu] T;Tobacco Research Institute of Hubei Province, Wuhan
, Hubei, China<&wdkj&>Tobacco Research Institute of Hubei Province, Wuhan
, Hubei, China
摘要：
BACKGROUND: Hydrogen sulfide (H(2)S) is a novel signaling molecule involved in the growth and development of plants and their response to stress. However, the involvement of H(2)S in promoting the growth and development of tobacco plants is still unclear. RESULTS: In this study, we explored the effect of pre-soaking or irrigating the roots of tobacco plants with 0.0, 2.0, 4.0, 6.0, and 8.0mM of sodium hydrosulfide (NaHS) on endogenous H(2)S production, antioxidant enzymatic and cysteine desulfhydrase activities, seed germination, agronomic traits, photosynthetic pigments contents, and root vigor. The results revealed that exogenous NaHS treatment could significantly promote endogenous H(2)S production by inducing gene expression of D/L-CD and the activities of D/L-CD enzymes. Additionally, a significant increase in the agronomic traits and the contents of photosynthetic pigments, and no significant difference in carotenoid content among tobacco plants treated with 0.0 to 8.0mM of NaHS was observed. Additionally, a significant increase in the germination speed, dry weight, and vigor of tobacco seeds, whereas no significant effect on the percentage of seed germination was observed on NaHS treatment. Furthermore, NaHS treatment could significantly increase the activity of superoxide dismutase (SOD) and peroxidase (POD) enzymes, which reduces damage due to oxidative stress by maintaining reactive oxygen species homeostasis. CONCLUSIONS: These results would aid in enhancing our understanding of the involvement of H(2)S, a novel signaling molecule to promote the growth and development of tobacco plants.
摘要：
Botrytis cinerea is a necrotrophic fungal plant pathogen that causes grey mould and rot diseases in many crops. Here, we show that the B. cinerea BcCrh4 transglycosylase is secreted during plant infection and induces plant cell death and pattern-triggered immunity (PTI), fulfilling the characteristics of a cell death-inducing protein (CDIP). The CDIP activity of BcCrh4 is independent of the transglycosylase enzymatic activity, it takes place in the apoplast and does not involve the receptor-like kinases BAK1 and SOBIR1. During saprophytic growth, BcCrh4 is localized in the endoplasmic reticulumand in vacuoles, but during plant infection, it accumulates in infection cushions (ICs) and is then secreted to the apoplast. Two domains within the BcCrh4 protein determine the CDIP activities: a 20aa domain at the N' end activates intense cell death and PTI, while a stretch of 52aa in the middle of the protein induces a weaker response and suppresses the activity of the 20aa N' domain. Deletion of bccrh4 affected fungal development and IC formation in particular, resulting in reduced virulence. Collectively, our findings demonstrate that BcCrh4 is required for fungal development and pathogenicity, and hint at a dual mechanism that balances the virulence activity of this, and potentially other CDIPs.
摘要：
Metabolic dysfunction is associated with nonalcoholic steatohepatitis (NASH) development. However, omics studies investigating metabolic changes in NASH patients are limited. In this study, metabolomics and lipidomics in plasma, as well as proteomics in the liver, were performed to characterize the metabolic profiles of NASH patients. Moreover, the accumulation of bile acids (BAs) in NASH patients prompted us to investigate the protective effect of cholestyramine on NASH. The liver expression of essential proteins involved in FA transport and lipid droplets was significantly elevated in patients with NASH. Furthermore, we observed a distinct lipidomic remodeling in patients with NASH. We also report a novel finding suggesting an increase in the expression of critical proteins responsible for glycolysis and the level of glycolytic output (pyruvic acid) in patients with NASH. Furthermore, the accumulation of branched chain amino acids, aromatic amino acids, purines, and BAs was observed in NASH patients. Similarly, a dramatic metabolic disorder was also observed in a NASH mouse model. Cholestyramine not only significantly alleviated liver steatosis and fibrosis but also reversed NASH-induced accumulation of BAs and steroid hormones. In conclusion, NASH patients were characterized by perturbations in FA uptake, lipid droplet formation, glycolysis, and accumulation of BAs and other metabolites.
期刊：
BioMed Research International,2023年2023:6540585 ISSN：2314-6133
通讯作者：
Yan Xiong<&wdkj&>Hongbo Cai<&wdkj&>Hongyu Zhang<&wdkj&>Feng Zhu<&wdkj&>Yang Xiang<&wdkj&>Rong Yan<&wdkj&>Xi Chen<&wdkj&>Xiaoyu Ke<&wdkj&>Shunmei Huang<&wdkj&>Weiran Ke<&wdkj&>Yu Han
作者机构：
[Xiong, Yan; Zhu, Feng; Yan, Rong; Cai, Hongbo; Han, Yu; Zhang, Hongyu; Xiang, Yang] School of Biological and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430030, China;[Ke, Weiran] College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China;[Chen, Xi] Department of Nosocomial Infection Management, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China;[Huang, Shunmei] Department of Geriatrics, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China;[Ke, Xiaoyu] Emergency Department and Intensive Care Unit, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
关键词：
Introduction;Materials and Methods;Results;Discussion;Conclusion;Abstract;Data Availability;Additional Points;Ethical Approval;Consent;Disclosure;Conflicts of Interests;Authors’ Contributions;Funding Statement;Acknowledgements;Acknowledgments;Supplementary Materials;Reference;Dataset Description;Dataset Files;Abstract;Introduction;Introduction and Materials;Introduction and Methods;Materials;Materials and Methods;Methods;Results;Discussion;Results and Discussion;Discussion and Conclusion;Results and Conclusion;Conclusion;Conclusions;Data Availability;Additional Points;Ethical Approval;Consent;Disclosure;Conflicts of Interest;Authors’ Contributions;Funding Statement;Acknowledgements;Supplementary Materials;References;Appendix;Abbreviations;Preliminaries;Introduction and Preliminaries;Notation;Proof of Theorem;Proofs;Analysis of Results;Examples;Numerical Example;Applications;Numerical Simulation;Model;Model Formulation;Systematic Palaeontology;Nomenclatural Acts;Taxonomic Implications;Experimental;Synthesis;Overview;Characterization;Background;Experimental;Theories;Calculations;Model Verification;Model Implementation;Geographic location;Study Area;Geological setting;Data Collection;Field Testing;Data and Sampling;Dataset;Literature Review;Related Works;Related Work;System Model;Methods and Data;Experimental Results;Results and Analysis;Evaluation;Implementation;Case Presentation;Case Report;Search Terms;Case Description;Case Series;Background;Limitations;Additional Points;Case;Case 1;Case 2 etc.;Concern Details;Retraction Details;Copyright;Related Articles
摘要：
BACKGROUND: Both high-fat diet (HFD) and 4-nonylphenol (4-NP) could affect fat formation in adipose tissue individually. We investigated whether HFD promote abnormal adipose tissue formation caused by early exposure to 4-NP in life and preliminarily explore the possible mechanisms involved. METHODS: The first-generation rats were treated with HFD on postnatal day after pregnant rats exposure to 5 ug/kg/day 4-NP. Then, the second generation rats started to only receive normal diet without 4-NP or HFD. We analyzed organ coefficient and histopathology of fat tissues, biochemical index, and gene level involved in lipid metabolism in female offspring rats. RESULTS: HFD and 4-NP interaction synergistically increased birth weight, body weight, and organ coefficients of adipose tissue in offspring female rats. HFD accelerately aggravated abnormal lipid metabolism and increased the adipocyte mean areas around the uterus of the offspring female rats induced by prenatal exposure to 4-NP. HFD also facilitate the regulation of gene expression involved lipid metabolism in offspring female rats induced by perinatal exposure to 4-NP, even passed on to the second generation of female rats. Moreover, HFD and 4-NP interaction synergistically declined the gene and protein expression of estrogen receptor (ER) in the adipose tissue of second-generation female rats. CONCLUSION: HFD and 4-NP synergistically regulate the expression of lipid metabolism genes in adipose tissue of F2 female rats and promote adipose tissue generation, leading to obesity in offspring rats, which is closely related to low expression of ER. Therefore, ER genes and proteins may be involved in the synergistic effect of HFD and 4-NP.
作者：
Bi, Kai;Liang, Yong;Mengiste, Tesfaye;Sharon, Amir
期刊：
Trends in Plant Science,2023年28(2):211-222 ISSN：1360-1385
通讯作者：
Amir Sharon
作者机构：
[Bi, Kai] Wuhan Polytech Univ, Coll Life Sci & Technol, Wuhan, Hubei, Peoples R China.;[Liang, Yong; Sharon, Amir] Tel Aviv Univ, Fac Life Sci, Sch Plant Sci & Food Secur, IL-69978 Tel Aviv, Israel.;[Mengiste, Tesfaye] Purdue Univ, Dept Bot & Plant Pathol, 915 West State St, W Lafayette, IN 47907 USA.
通讯机构：
[Amir Sharon] S;School of Plant Sciences and Food Security, Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel
摘要：
Orcinol glucoside (OG), mainly found in the rhizome of the traditional Chinese herb Curculigo orchioides Gaertn, is noted for its antidepressant effects. In this study, an efficient screening pipeline was established for identifying the highly active orcinol synthase (ORS) and UDP-dependent glycosyltransferase (UGT) involved in the biosynthesis of OG by combining transcriptome analysis, structure-based virtual screening, and in vitro enzyme activity assays. By enhancing the downstream pathway, metabolic engineering and fermentation optimization, the OG production in Yarrowia lipolytica was improved 100-fold, resulting in a final yield of 43.46 g/L (0.84 g/g DCW), which is almost 6,400-fold higher than the extraction yield from C. orchioides roots. This study provides a reference for rapid identification of functional genes and high-yield production of natural products.
期刊：
World Journal of Microbiology and Biotechnology,2023年39(3):1-12 ISSN：0959-3993
通讯作者：
Xiaomin Hu<&wdkj&>Hairong Xiong
作者机构：
[Wang, Yawei] Wuhan Polytech Univ, Coll Life Sci & Technol, Wuhan 430048, Peoples R China.;[Xiong, Hairong; Hu, Xiaomin; Zhao, Puying] South Cent Univ Nationalities, Coll Life Sci, Wuhan 430074, Peoples R China.;[Zhou, Ying] Wuhan Sunhy Biol Co Ltd, Wuhan 430205, Peoples R China.
通讯机构：
[Xiaomin Hu; Hairong Xiong] C;College of Life Science, South-Central University for Nationalities, Wuhan, China<&wdkj&>College of Life Science, South-Central University for Nationalities, Wuhan, China
摘要：
Protein hydrolysates are easily digested and utilized by humans and animals, and are less likely to cause allergies. Protein hydrolysis caused by endopeptidases often leads to the exposure of hydrophobic amino acids at the ends of peptides, which consequently causes bitter taste. Microbial aminopeptidases remove the exposed hydrophobic amino acids at the ends of aminopeptides, which improves taste, allowing for easier production. This processe is attacking significant attention from industry and laboratories. Aminopeptidases selectively hydrolyze peptide bonds from the N-terminal of proteins or peptides to produce free amino acids. Aminopeptidases can be classified into leucine, lysine, methionine and proline aminopeptidases by hydrolyzed N-terminal residues; metallo-, serine- and cysteine- aminopeptidases by the reaction mechanisms; dipeptide and triphoptide enzymes by the released number of amino acid residues at the end of hydrolyzed peptides; or acidic, neutral and basic aminopeptidases by their optimal hydrolysis pH. Commercial aminopeptidases are generally produced by microbial fermentation, and are mainly applied in the debittering of protein hydrolysates, the deep hydrolysis of protein, and the production of condiments, cheese, and bioactive peptides, as well as for disease detection in the medical industry.
作者机构：
[骆健美; 黄蓉; 白杰; 张鹤渐; 李从雨] College of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China;[刘晓楠; 黄蓉; 江会锋; 王千; 白杰; 张鹤渐; 李从雨; 张磊] Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China;[张磊] College of Life Science and Technology, Wuhan Polytechnic University, Wuhan 430023, Hubei, China
摘要：
Zearalenone (ZEN) is one of the most prevalent estrogenic mycotoxins, is produced mainly by the Fusarium family of fungi, and poses a risk to the health of animals. Zearalenone hydrolase (ZHD) is an important enzyme capable of degrading ZEN into a non-toxic compound. Although previous research has investigated the catalytic mechanism of ZHD, information on its dynamic interaction with ZEN remains unknown. This study aimed to develop a pipeline for identifying the allosteric pathway of ZHD. Using an identity analysis, we identified hub genes whose sequences can generalize a set of sequences in a protein family. We then utilized a neural relational inference (NRI) model to identify the allosteric pathway of the protein throughout the entire molecular dynamics simulation. The production run lasted 1 microsecond, and we analyzed residues 139-222 for the allosteric pathway using the NRI model. We found that the cap domain of the protein opened up during catalysis, resembling a hemostatic tape. We used umbrella sampling to simulate the dynamic docking phase of the ligand-protein complex and found that the protein took on a square sandwich shape. Our energy analysis, using both molecular mechanics/Poisson-Boltzmann (Generalized-Born) surface area (MMPBSA) and Potential Mean Force (PMF) analysis, showed discrepancies, with scores of -8.45 kcal/mol and -1.95 kcal/mol, respectively. MMPBSA, however, obtained a similar score to that of a previous report.
摘要：
Zymomonas mobilis is an important industrial bacterium ideal for biorefinery and synthetic biology studies. High-throughput CRISPR-based genome editing technologies have been developed to enable targeted engineering of genes and hence metabolic pathways in the model ZM4 strain, expediting the exploitation of this biofuel-producing strain as a cell factory for sustainable chemicals, proteins and biofuels production. As these technologies mainly take plasmid-based strategies, their applications would be impeded due to the fact that curing of the extremely stable plasmids is laborious and inefficient. Whilst counterselection markers have been proven to be efficient for plasmid curing, hitherto only very few counterselection markers have been available for Z. mobilis. We constructed a conditional lethal mutant of the pheS gene of Z. mobilis ZM4, clmPheS, containing T263A and A318G substitutions and coding for a mutated alpha-subunit of phenylalanyl-tRNA synthetase to allow for the incorporation of a toxic analog of phenylalanine, p-chloro-phenylalanine (4-CP), into proteins, and hence leading to inhibition of cell growth. We demonstrated that expression of clmPheS driven by a strong Pgap promoter from a plasmid could render the Z. mobilis ZM4 cells sufficient sensitivity to 4-CP. The clmPheS-expressing cells were assayed to be extremely sensitive to 0.2 mM 4-CP. Subsequently, the clmPheS-assisted counterselection endowed fast curing of genome engineering plasmids immediately after obtaining the desired mutants, shortening the time of every two rounds of multiplex chromosome editing by at least 9 days, and enabled the development of a strategy for scarless modification of the native Z. mobilis ZM4 plasmids. This study developed a strategy, coupling an endogenous CRISPR-based genome editing toolkit with a counterselection marker created here, for rapid and efficient multi-round multiplex editing of the chromosome, as well as scarless modification of the native plasmids, providing an improved genome engineering toolkit for Z. mobilis and an important reference to develope similar genetic manipulation systems in other non-model organisms.
期刊：
Ecotoxicology and Environmental Safety,2022年240:113698 ISSN：0147-6513
通讯作者：
Xiaochun Guo<&wdkj&>Dongru Qiu
作者机构：
[Liu, Ying; Wan, Zhengfen; Guo, Xiaochun; Lu, Shaoyong; Liu, Xiaohui] Chinese Res Inst Environm Sci, State Key Lab Environm Criteria & Risk Assessment, Natl Engn Lab Lake Pollut Control & Ecol Restorat, Beijing 100012, Peoples R China.;[Liu, Xiaohui] Tsinghua Univ, Sch Environm, Beijing 100084, Peoples R China.;[Chen, Jing] Wuhan Polytech Univ, Coll Life Sci & Technol, Wuhan 430070, Peoples R China.;[Chen, Jing; Qiu, Dongru] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
通讯机构：
[Xiaochun Guo] S;[Dongru Qiu] I;Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China<&wdkj&>State Key Laboratory of Environmental Criteria and Risk Assessment, National Engineering Laboratory for Lake Pollution Control and Ecological Restoration, Chinese Research Academy of Environmental Sciences, Beijing 100012, China
摘要：
Feruloyl esterase is a subclass of α/β hydrolase, which could release ferulic acid from biomass residues for use as an efficient additive in food or pharmaceutical industries. In the present study, a feruloyl esterase with broad substrate specificity was characterised and secreted by Bacillus subtilis WB600. After codon usage optimisation and signal peptide library screening, the secretion amount of feruloyl esterase was enhanced by up to 10.2-fold in comparison with the base strain. The site-specific amino acid substitutions that facilitate protein folding further improved the secretion by about 1.5-fold. The purified rationally designed enzyme exhibited maximal activity against methyl ferulate at pH 6.5 and 65 °C. In the solid-state fermentation, the genetically engineered B. subtilis released about 37% of the total alkali-extractable ferulic acid in maize bran. This study provides a promising candidate for ferulic acid production and demonstrates that the secretion of a heterologous enzyme from B. subtilis can be cumulatively improved by changes in protein sequence features.
摘要：
Lotus root (Nelumbo nucifera G.) is a high economic value crop in the world. In this study, the storage characteristics (color, sensory, texture, and fatty acids) of lotus root ("Elian No.5") were evaluated at different harvest periods (September 2018, October 2018, November 2018, December 2018, and January 2019). Moreover, the storage characteristics were evaluated after the shortterm and long-term storage of lotus root at 4 degrees C and 20 degrees C. The hardness of lotus root significantly decreased at both temperatures (4 degrees C and 20 degrees C) during the first 3 days of storage. In contrast, the decrease in hardness delayed at 4 degrees C (beyond 3 days of storage). Further, genes related to hardness at different storage temperatures were identified using the RNA-seq and qRT-PCR. The results of this study provide a reference for lotus root storage and a basis for the molecular breeding of longterm-storable lotus root.
期刊：
Life Sciences,2021年265(2):118761 ISSN：0024-3205
通讯作者：
Huang, Kun
作者机构：
[Li, Yang] Wuhan Polytech Univ, Coll Biol & Pharmaceut Engn, Wuhan 430023, Peoples R China.;[Xiao, Yushuo] Hubei Univ Chinese Med, Sch Pharm, Wuhan 430065, Peoples R China.;[Chen, Yuchen; Huang, Kun] Huazhong Univ Sci & Technol, Sch Pharm, Tongji Med Coll, Wuhan 430030, Peoples R China.;[Huang, Kun] Huazhong Univ Sci & Technol, Tongji Sch Pharm, Tongji Med Coll, Wuhan 430030, Peoples R China.
通讯机构：
[Huang, Kun] H;Huazhong Univ Sci & Technol, Tongji Sch Pharm, Tongji Med Coll, Wuhan 430030, Peoples R China.
关键词：
Virus;Infection diseases;Nanomaterials;Antiviral agents and mechanisms;Nanovaccines
摘要：
Outbreaks and the rapid transmission of viruses, such as coronaviruses and influenza viruses, are serious threats to human health. A major challenge in combating infectious diseases caused by viruses is the lack of effective methods for prevention and treatment. Nanotechnology has provided a basis for the development of novel antiviral strategies. Owing to their large modifiable surfaces that can be functionalized with multiple molecules to realize sophisticated designs, nanomaterials have been developed as nanodrugs, nanocarriers, and nano-based vaccines to effectively induce sufficient immunologic memory. From this perspective, we introduce various nanomaterials with diverse antiviral mechanisms and summarize how nano-based antiviral agents protect against viral infection at the molecular, cellular, and organismal levels. We summarize the applications of nanomaterials for defense against emerging viruses by trapping and inactivating viruses and inhibiting viral entry and replication. We also discuss recent progress in nano-based vaccines with a focus on the mechanisms by which nanomaterials contribute to immunogenicity. We further describe how nanotechnology may improve vaccine efficacy by delivering large amounts of antigens to target immune cells and enhancing the immune response by mimicking viral structures and activating dendritic cells. Finally, we provide an overview of future prospects for nano-based antiviral agents and vaccines.
期刊：
Ecotoxicology and Environmental Safety,2021年221:112427 ISSN：0147-6513
通讯作者：
Liu, X.;Lu, S.
作者机构：
[Wang, Yongqiang; Lu, Shaoyong; Liu, Xiaohui] Chinese Res Inst Environm Sci, Res Ctr Lake Environm,State Environm Protect Key, State Key Lab Environm Criteria & Risk Assessment, Natl Engn Lab Lake Pollut Control & Ecol Restorat, Beijing 100012, Peoples R China.;[Wang, Yongqiang] Harbin Inst Technol, State Key Lab Urban Water Resource & Environm, Harbin 150090, Peoples R China.;[Liu, Xiaohui] Tsinghua Univ, Beijing 100084, Peoples R China.;[Chen, Jing] Wuhan Polytech Univ, Coll Biol & Pharmaceut Engn, Wuhan 430023, Peoples R China.;[Han, Maozhen] Anhui Med Univ, Sch Life Sci, Hefei 230032, Peoples R China.
通讯机构：
[Shaoyong Lu; Xiaohui Liu] S;State Key Laboratory of Environmental Criteria and Risk Assessment, National Engineering Laboratory for Lake Pollution Control and Ecological Restoration, State Environmental Protection Key Laboratory for Lake Pollution Control, State Environmental Protection Scientific Observation and Research Station for Lake Dongtinghu (SEPSORSLD), Research Centre of Lake Environment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China<&wdkj&>Tsinghua University, Beijing 100084, China<&wdkj&>State Key Laboratory of Environmental Criteria and Risk Assessment, National Engineering Laboratory for Lake Pollution Control and Ecological Restoration, State Environmental Protection Key Laboratory for Lake Pollution Control, State Environmental Protection Scientific Observation and Research Station for Lake Dongtinghu (SEPSORSLD), Research Centre of Lake Environment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China
关键词：
Ebinur Lake Basin;Antibiotics;Antibiotic resistance genes;Microbial community
作者机构：
[Lu, Jian; Liu, Tian] Guangxi Univ, Life Sci & Technol Coll, Nanning 530004, Guangxi, Peoples R China.;[Lu, Lina; Liu, Yuqian; Jiang, Huifeng; Liu, Tian; Guo, Zhaokuan; Liu, Xiaonan; Zhu, Xiaoxi; Cheng, Jian] Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China.;[Lu, Lina; Liu, Yuqian; Jiang, Huifeng; Liu, Tian; Guo, Zhaokuan; Liu, Xiaonan; Cheng, Jian] Natl Ctr Technol Innovat Synthet Biol, Tianjin 300308, Peoples R China.;[Liu, Yuqian] South China Univ Technol, Sch Biol & Biol Engn, Guangzhou 510006, Guangdong, Peoples R China.;[Fan, Guanwei; Li, Lan] Tianjin Univ Tradit Chinese Med, Teaching Hosp 1, Tianjin 300193, Peoples R China.
通讯机构：
[Xiaonan Liu; Huifeng Jiang] K;[Nengzhong Xie] N;[Jian Lu] L;Life Science and Technology College, Guangxi University, Nanning, Guangxi 530004, China<&wdkj&>Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China<&wdkj&>National Center of Technology Innovation for Synthetic Biology, Tianjin 300308, China<&wdkj&>Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China<&wdkj&>University of Chinese Academy of Sciences, Beijing 100049, China<&wdkj&>National Center of Technology Innovation for Synthetic Biology, Tianjin 300308, China<&wdkj&>National Engineering Research Center for Non-Food Biorefinery, State Key Laboratory of Non-Food Biomass and Enzyme Technology, Guangxi Biomass Engineering Technology Research Center, Guangxi Academy of Sciences, Nanning, Guangxi 530007, China
摘要：
Polydatin, with better structural stability and biological activities than resveratrol, is mainly extracted from the traditional Chinese medicinal plant Polygonum cuspidatum. In this study, based on the transcriptome analysis of P. cuspidatum, we identified the key glycosyltransferase of resveratrol and achieved the biosynthesis of polydatin from glucose by incorporation with the resveratrol biosynthesis module, UDP-glucose supply module, and glycosyltransferase expression module. Through metabolic engineering and fermentation optimization, the production of polydatin reached 545 mg/L, and the dry cell weight was 27.83 mg/g DCW, which was about twice that of extracted from the P. cuspidatum root (11.404 mg/g DCW). Therefore, it is possible to replace the production mode of polydatin from plant extraction to microbial chassis in the future.
关键词：
anthraquinone;azo dye;low-voltage;pulse electrolysis;Reactive Blue 19
摘要：
Wastewater produced by the textile industry containing azo dyes and anthraquinone dyes is significant source of pollution to the environment and is toxic for aquatic life. To overcome the high-energy cost of traditional electrochemical oxidation, a custom-built power supply device for the degradation of anthraquinone and azo dyes by low voltage of 15.0-20.0 V pulsed discharge was investigated. Titanium coated with mixed oxide (Ti/IrO2 -RuO2 -SnO2 ) plates and pure titanium plates were used as the anode and cathode, respectively, for the generation of chlorine in the dye solution. For the anthraquinone dye Reactive Blue 19, 60.0% of the chemical oxygen demand (COD) and 22.0% of the total organic carbon (TOC) were removed using this system. A comparison of the direct current electrolysis and pulsed discharge revealed that using the pulsed discharge method reduced the energy cost by 68.6%. UV-visible, LC-MS, and GC-MS were used to identify the intermediate compounds formed during the degradation of Reactive Blue 19. The results indicate that in the process of oxidation by chlorine/hypochlorite, the chromophore group was first oxidized to -NH2 , followed by decolorization via chlorination of the aromatic rings. The results confirm that low-voltage pulse electrolysis can be used for the degradation of industrial dyes in waste effluents. PRACTITIONER POINTS: Low-voltage pulse electrolysis can be used for the degradation of industrial dyes and/or dyes in waste effluents. For anionic dye Reactive Blue 19, 60.0% of COD and 22.0% of TOC were removed using low-voltage (20.0 V) pulse electrolysis. The pulsed discharge method reduced the energy cost of this degradation process by 68.6% compared with direct current electrolysis. The intermediate compounds formed during the degradation of Reactive Blue 19 were confirmed by UV-visible spectroscopy, LC-MS, and GC-MS.
摘要：
A reduction in the use of aluminum (Al)-based flocculants in the treatment of drinking water is considered essential for human health reasons. In this study, a novel composite flocculant, made of carboxymethylpullulan-AlCl3 , is evaluated in a lab-scale, jar test system for the flocculation of kaolin. The results showed that the coagulation efficiency of carboxymethylpullulan-AlCl3 was more effective in reducing turbidity than the solo use of carboxymethylpullulan or AlCl3 . The optimum treatment conditions assessed by a response surface methodology were obtained at pH 6.50, 13.03 mg/L carboxymethylpullulan, and 94.87 mg/L AlCl3 . Zeta potential measurements and photometric dispersion analysis demonstrated that AlCl3 had a more significant influence on charge neutralization than carboxymethylpullulan, whilst carboxymethylpullulan facilitated absorption and the development of particle bridges. Thus, the composite flocculant possessed both advantages that enhanced flocculation, and decreased the dosage of AlCl3 , thereby reducing the potential for secondary environment pollution. When 90 mg/L carboxymethylpullulan-AlCl3 was added to the model kaolin suspension characterized by a turbidity of 50 nephelometric turbidity units, the zeta potential and the maximum flocculating activity were determined as -2.28 mV and 98.0%, respectively. The results provide insight into the development of an environment-friendly composite flocculant prepared from water-dissolved polysaccharide and inorganic flocculants. PRACTITIONER POINTS: A novel composite flocculant CMP-AlCl3 was achieved by combining CMP and AlCl3 for water treatment. The coagulation efficiency of CMP-AlCl3 was more effective in reducing turbidity than the solo use of CMP or AlCl3 . The flocculation efficiency and mechanism were investigated by Zeta potential analysis, surface morphology, electron microscopy, and coagulation.
