三丁酸甘油酯与三乳酸甘油酯对脂多糖刺激肉鸡生长性能、血液生化指标及肝脏能量状态的影响
作者:
李娇龙;张俊;邱洪仪;杨玲;谭灵琳;...
期刊:
饲料工业 ,2014年35(18):38-43 ISSN:1001-991X
作者机构:
武汉轻工大学动物营养与饲料科学湖北省重点实验室;[杨玲] 武汉泛华生物技术有限公司;[丁斌鹰; 李娇龙; 谭灵琳; 张俊; 邱洪仪] 武汉轻工大学
关键词:
三丁酸甘油酯;三乳酸甘油酯;生长性能;能量状态;脂多糖;肉鸡
摘要:
试验研究了三丁酸甘油酯与三乳酸甘油酯对1~21日龄肉鸡生产性能和对脂多糖(LPS)刺激肉鸡生长性能、血浆GPT、GOT、AKP活性以及肝脏能量状态的影响.试验分2期.第Ⅰ期:选取240只体重基本一致的1日龄Cobb肉公鸡,随机分成3组(对照组、丁酸酯组和乳酸酯组),每组4个重复,每个重复20只鸡.其中对照组饲喂基础日粮,丁酸酯组和乳酸酯组分别饲喂在基础日粮的基础上添加0.1%三丁酸甘油酯、0.1%三乳酸甘油酯的试验日粮.第Ⅱ期:在试验第22 d,试验分为4组,分别为对照组和LPS组(选自试验Ⅰ期对照组,各10只鸡)、丁酸酯组和乳酸酯组(从试验Ⅰ期丁酸酯组和乳酸酯组各选取10只鸡).其中对照组和LPS组饲喂基础日粮,丁酸酯组和乳酸酯组分别饲喂在基础日粮的基础上添加0.1%三丁酸甘油酯、0.1%三乳酸甘油酯的试验日粮.每个组10个重复,每个重复1只鸡.在试验第25、27、29 d上午8:00,对LPS组、丁酸酯组和乳酸酯组的试验肉鸡分别腹腔注射500 μg/kg体重的LPS,对照组注射相同剂量的灭菌生理盐水.第29 d中午12:00采血,第30 d上午8:00屠宰取肝脏.试验Ⅰ期结果显示:饲粮中添加0.1%的三丁酸甘油酯和三乳酸甘油酯对1~21日龄肉鸡生长性能均无显著性影响(P>0.05).试验Ⅱ期结果显示:①饲粮中添加三丁酸甘油酯和三乳酸甘油酯均缓解了LPS刺激引起了日增重的降低(P>0.05),且二种甘油三酯之间无显著性差异(P>0.05).②饲粮中添加三丁酸甘油酯和三乳酸甘油酯均缓解了LPS刺激导致的血浆谷丙转氨酶(GPT)、谷草转氨酶(GOT)的升高(P<0.05),但二种甘油三酯之间并无显著性差异(P>0.05).③饲粮中添加三丁酸甘油酯和三乳酸甘油酯均显著缓解了LPS刺激引起的三磷酸腺苷(ATP)、二磷酸腺苷(AMP)、腺苷酸池(TAN)、能荷(EC)的降低(P<0.05)和AMP/ATP的升高(P<0.05).三乳酸甘油酯还显著升高了AMP水平(P<0.05).由此可见,在本试验条件下,饲粮中添加0.1%的三丁酸甘油酯和三乳酸甘油酯均缓解了LPS刺激引起的肝脏损伤.
语种:
中文
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mTOR信号通路在谷氨酰胺调控猪肠上皮细胞生长中的作用
作者:
欧阳万金;肖航;侯永清;龙民慧;丁斌鹰;...
作者机构:
[欧阳万金; 肖航; 侯永清; 龙民慧; 丁斌鹰; 易丹; 王蕾] 武汉轻工大学动物营养与饲料科学湖北省重点实验室,武汉430023
会议名称:
中国畜牧兽医学会动物营养学分会第七届中国饲料营养学术研讨会
会议时间:
2014-10-16
会议地点:
郑州
会议主办单位:
中国畜牧兽医学会动物营养学分会
会议论文集名称:
中国畜牧兽医学会动物营养学分会第七届中国饲料营养学术研讨会论文集
关键词:
谷氨酰胺;猪小肠上皮细胞;mTOR信号通路
摘要:
谷氨酰胺(L-glutamine,Gln)是肠细胞和免疫细胞等细胞快速分化的优先底物,可刺激细胞增殖,能调控基因表达和细胞信号通路。Gln不仅作为肠上皮细胞的主要能量物质,为小肠上皮细胞的分化和增殖提供能量,而且还可通过调节谷氨酰胺酶活性、相关细胞信号通路和蛋白合成等促进肠上皮细胞的增殖,但其作用机理还不清楚。mTOR(哺乳动物雷帕霉素靶蛋白,mammalian target of rapamyc
语种:
中文
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Dietary supplementation with tributyrin alleviates intestinal injury in piglets challenged with intrarectal administration of acetic acid
作者:
Hou, Yongqing* ;Wang, Lei;Yi, Dan;Ding, Binying;Chen, Xing;...
期刊:
British Journal of Nutrition ,2014年111(10):1748-1758 ISSN:0007-1145
通讯作者:
Hou, Yongqing
作者机构:
[Liu, Yulan; Hou, Yongqing; Chen, Xing; Wang, Lei; Wang, Qingjing; Ding, Binying; Yin, Yulong; Yi, Dan; Zhu, Huiling] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Gong, Joshua] Agr & Agri Food Canada, Guelph Food Res Ctr, Guelph, ON N1G 5C9, Canada.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.
通讯机构:
[Hou, Yongqing] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
关键词:
Acetic acid;Colon;Intestinal injury;Piglets;Tributyrin
摘要:
Tributyrin (TBU) is a good dietary source of butyrate and has beneficial effects on the maintenance of normal intestinal morphology. The present study tested the hypothesis that dietary TBU supplementation could alleviate intestinal injury in the acetic acid (ACA)-induced porcine model of colitis. A total of eighteen piglets (25Â d old) were randomly allocated to one of three treatment groups (control, ACA and TBU). The control and ACA groups were fed a basal diet and the TBU group was fed the basal diet supplemented with 0·1Â % TBU. On day 15 of the trial, under anaesthesia, a soft catheter was inserted into the rectum of piglets (20-25Â cm from the anus), followed by administration of either saline (control group) or ACA (10Â ml of 10Â % ACA solution for ACA and TBU groups). On day 22 of the trial, after venous blood samples were collected, piglets were killed to obtain mid-ileum and mid-colon mucosae. Compared with the control group, the ACA group exhibited an increase (P<Â 0·05) in lymphocyte counts, creatinine, PGE2, and malondialdehyde concentrations and diamine oxidase and inducible NO synthase activities in the plasma and lymphocyte density in the colon and a decrease in insulin concentrations and glutathione peroxidase activity, ileal villus height:crypt depth ratios and goblet cell numbers in the colon. These adverse effects of ACA were attenuated by TBU supplementation. Moreover, TBU prevented the ACA-induced increase in caspase-3 levels while enhancing claudin-1 protein and epidermal growth factor receptor (EGFR) mRNA expression in the colonic mucosa. Collectively, these results indicate that dietary supplementation with 0·1Â % TBU alleviates ACA-induced intestinal injury possibly by inhibiting apoptosis, promoting tight-junction formation and activating EGFR signalling. © 2014 The Authors.
语种:
英文
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几种氨基酸及其衍生物对猪肠上皮细胞IPEC-1细胞活力与生长的影响
作者:
欧阳万金;龙民慧;侯永清;肖航;丁斌鹰;...
期刊:
饲料工业 ,2014年35(3):42-46 ISSN:1001-991X
作者机构:
动物营养与饲料科学湖北省重点实验室武汉轻工大学,湖北武汉,430023
关键词:
氨基酸及其衍生物;细胞活力
摘要:
研究L-谷氨酰胺(L-glutamine,GLN),Nα-乙酰-L-谷氨酰胺(Nα-acetyl-L-glutamine,NAG),L-精氨酸(L-arginine,ARG),N-α-乙酰-L-精氨酸(N-alpha-acetyl-L-arginine,NAA)、瓜氨酸(L-citrulline,CIT),α-酮戊二酸(Alpha-ketoglutaric acid,AKG)和L-脯氨酸(L-Proline,PRO)对猪小肠上皮细胞(Intestinal porcine epithelial cells,IPEC-1)细胞活力与生长的影响,比较这几种氨基酸及其衍生物对IPEC-1细胞生长的作用效果。在IPEC-1定制培养液中分别添加不同浓度的氨基酸或其衍生物培养4 d,用CCK8(cell counting kit 8)法检测细胞的活力,并用细胞计数法检测这些氨基酸或其衍生物对细胞生长的影响。试验的整个4 d,GLN组IPEC-1细胞的数量(P<0.05)和OD值(P<0.05)均显著高于其他组。在试验的第4 d,NAG、AKG、CIT、NAA和ARG组细胞数量均高于对照组(P<0.05或0.01)。培养液中添加PRO对IPEC-1细胞的生长无明显影响。①在这几种氨基酸及其衍生物中,GLN促进IPEC-1细胞生长的作用效果最好。②NAG、AKG、CIT、NAA或ARG均能提高第4 d IPEC-1细胞的数量。
语种:
中文
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三丁酸甘油酯与三乳酸甘油酯对脂多糖刺激肉鸡生长性能、血浆生化指标及肝脏能量状态的影响
作者:
李娇龙;王蕾;张俊;邱洪仪;杨玲;...
作者机构:
[李娇龙; 王蕾; 张俊; 邱洪仪; 谭灵琳; 丁斌鹰] 武汉轻工大学动物营养与饲料科学湖北省重点实验室,武汉430023;[杨玲] 武汉泛华生物技术有限公司,武汉430023
会议名称:
中国畜牧兽医学会动物营养学分会第七届中国饲料营养学术研讨会
会议时间:
2014-10-16
会议地点:
郑州
会议主办单位:
中国畜牧兽医学会动物营养学分会
会议论文集名称:
中国畜牧兽医学会动物营养学分会第七届中国饲料营养学术研讨会论文集
关键词:
三丁酸甘油酯;三乳酸甘油酯;肉鸡;生长性能;能量状态;脂多糖
摘要:
来自饲养环境中的各种因素会对肉鸡生产中造成应激,使其身体机能发生紊乱,生产性能下降,从而对肉鸡生产造成严重的经济损失。脂多糖(LPS)是革兰氏阴性细菌细胞壁特有的结构成分,能够造成机体免疫应激。三丁酸甘油酯和三乳酸甘油酯分别是由丁酸和乳酸与甘油酯化而成的。二者都被证明具有良好的抑菌效果,能抑制炎性因子分泌,还能作为能源物质为机体供能。本试验用LPS多次刺激肉鸡建立应激模型,研究了三丁酸甘油酯与三乳
语种:
中文
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N-乙酰半胱氨酸对脂多糖刺激仔猪血浆中激素水平的影响
作者:
肖航;方秋红;侯永清;丁斌鹰;王蕾;...
作者机构:
[肖航; 方秋红; 侯永清; 丁斌鹰; 王蕾; 陈振强] 武汉轻工大学动物营养与饲料科学湖北省重点实验室,武汉430023
会议名称:
中国畜牧兽医学会动物营养学分会第七届中国饲料营养学术研讨会
会议时间:
2014-10-16
会议地点:
郑州
会议主办单位:
中国畜牧兽医学会动物营养学分会
会议论文集名称:
中国畜牧兽医学会动物营养学分会第七届中国饲料营养学术研讨会论文集
关键词:
N-乙酰半胱氨酸;脂多糖;仔猪;血浆;激素水平
摘要:
动物的生长是由促生长激素轴来调控的,促生长激素轴由生长激素释放因子(GRF)、生长激素(GH)和胰岛素样生长因子(IGF)构成,与动物的生长相关。IGF-1能够直接作用于动物体内的多种组织,促进蛋白质合成,促进细胞增殖,达到促进生长的作用。INS(胰岛素)是促进合成代谢、调节血糖稳定的主要激素,有促进动物体内蛋白质合成的作用。INS和IGF-1的水平提高意味着动物体合成代谢增强。胰高血糖素是促进分
语种:
中文
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天冬酰胺对脂多糖刺激仔猪肠黏膜结构及抗氧化能力的影响
作者:
皮定安;刘玉兰;李爽;石海峰;朱惠玲;...
期刊:
中国畜牧杂志 ,2014年50(05):60-64 ISSN:0258-7033
作者机构:
武汉轻工大学动物营养与饲料科学湖北省重点实验室,湖北武汉,430023;[丁斌鹰; 侯永清; 李爽; 冷炜博; 陈逢; 朱惠玲; 刘玉兰; 皮定安; 石海峰] 武汉轻工大学
关键词:
天冬酰胺;脂多糖;肠黏膜结构;抗氧化;仔猪
摘要:
试验旨在研究天冬酰胺(Asn)对脂多糖(LPS)刺激仔猪肠道结构及抗氧化能力的影响。选用24头断奶仔猪,分为对照组、LPS组、0.5%Asn组(0.5%Asn+LPS)和1.0%Asn组(1.0%Asn+LPS)。第24天,各组分别注射LPS或等量生理盐水24 h后屠宰。结果表明:LPS刺激导致空肠绒毛高度显著降低,日粮中添加0.5%或1.0%Asn显著降低了空肠、回肠的隐窝深度,提高了空肠、回肠的绒毛高度和绒毛高度/隐窝深度的比值(P<0.05);LPS刺激也导致空肠超氧化物歧化酶(SOD)活性和氧化型谷胱甘肽(GSSG)/还原性谷胱甘肽(GSH)比值显著升高(P<0.05);日粮中添加0.5%或1.0%Asn显著提高了空肠、回肠GSH的含量(P<0.05),降低了空肠、回肠GSSG/GSH的比值(P<0.01)和丙二醛(MDA)的含量(P<0.05)。结果显示,Asn缓解了LPS刺激引起的肠道结构损伤和抗氧化能力下降。
语种:
中文
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Effects of l-proline on the growth performance, and blood parameters in weaned lipopolysaccharide (LPS)-challenged pigs
作者:
Kang, Ping;Zhang, Lili;Hou, Yongqing* ;Ding, Binying;Yi, Dan;...
期刊:
Animal Bioscience ,2014年27(8):1150-1156 ISSN:2765-0189
通讯作者:
Hou, Yongqing
作者机构:
[Zhang, Lili; Liu, Yulan; Kang, Ping; Hou, Yongqing; Wang, Lei; Ding, Binying; Yin, Yulong; Yi, Dan; Zhu, Huiling] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Yin, Yulong] Chinese Acad Sci, Inst Subtrop Agr, Changsha 410125, Hunan, Peoples R China.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.
通讯机构:
[Hou, Yongqing] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
关键词:
Kang, Ping;Zhang, Lili;Hou, Yongqing;Ding, Binying;Yi, Dan;Wang, Lei;Zhu, Huiling;Liu, Yulan;Yin, Yulong;Wu, Guoyao;L-proline;Growth Performance;Blood Parameters;Gastrointestinal Tract Digestibility;Early Weaned Pigs;Lipopolysaccharide
摘要:
This trail was conducted to study the effect of L-proline on the growth performance, and blood parameter in the weaned lipopolysaccharide (LPS)-challenged pigs. Thirty six pigs (9.13±0.85 kg) were assigned randomly to dietary treatments in a 2×3 factorial arrangement in a 20-d growth assay. Factors were intraperitoneal injection with saline or LPS, and three dietary L-proline supplement levels (0%, 0.5%, or 1.0%). On d 10, blood samples were collected at 3 h after LPS (100 μg LPS/kg body weight [BW]) or saline injection. On d 20 of the trial, all pigs were orally administrated D-xylose (0.1 g/kg BW) at 2 h, and blood samples were collected at 3 h after LPS or saline injection. As a result, dietary supplementation with 0.5% proline had a tendency to increase average daily gain (ADG) in piglets during d 10 to 20 (p = 0.088). Without LPS challenge, dietary supplementation with 1.0% proline had no effect on growth hormone (GH) concentrations on d 10 (p>0.05), but decreased it after LPS challenge (p<0.05). There was LPS challenge× proline interaction for GH concentrations on d 10 (p<0.05). Dietary supplementation with 1.0% proline decreased glucagon concentration on d 10 after LPS challenge (p<0.05). In addition, dietary supplementation with proline increased superoxide dismutase (SOD) activity significantly on d 10 and 20 (p<0.05), and 1.0% proline increased heat shock proteins-70 concentration on d 10 (p<0.05). Moreover, proline supplementation increased diamine oxidase (DAO) concentrations after LPS challenge (p<0.05). There was LPS challenge×proline interaction for DAO (p<0.05). Furthermore, dietary supplementation with 1.0% proline increased the D-xylose level when no LPS challenge (p<0.05). These results indicate that proline supplementation could improve growth performance, increase SOD activities, and has a positive effect on the gastrointestinal tract digestibility in early weaned pigs. © 2014 by Asian-Australasian Journal of Animal Sciences.
语种:
英文
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Dietary N-acetylcysteine supplementation alleviates liver injury in lipopolysaccharide-challenged piglets
作者:
Yi, Dan;Hou, Yongqing* ;Wang, Lei;Ding, Binying;Yang, Zhengguo;...
期刊:
British Journal of Nutrition ,2014年111(1):46-54 ISSN:0007-1145
通讯作者:
Hou, Yongqing
作者机构:
[Long, Minhui; Liu, Yulan; Yang, Zhengguo; Hou, Yongqing; Wang, Lei; Ding, Binying; Li, Jiao; Yi, Dan] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.;[Wu, Guoyao] China Agr Univ, State Key Lab Anim Nutr, Beijing 100193, Peoples R China.
通讯机构:
[Hou, Yongqing] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
关键词:
N-Acetylcysteine;Piglets;Lipopolysaccharide;Liver injury
摘要:
The present study was carried out to determine whether N-acetylcysteine (NAC) could modulate liver injury in a lipopolysaccharide (LPS)-challenged piglet model. For this purpose, eighteen piglets were randomly assigned to the control, LPS or NAC group. Piglets in the control and LPS groups were fed a basal diet, whereas those in the NAC group were fed the basal diet supplemented with 500mg/kg NAC. On days 10, 13 and 20 of the trial, the LPS-and NAC-treated piglets were intraperitoneally administered LPS (100μg/kg body weight), while the control group was administered the same volume of saline. On day 20 of the trial, blood samples were obtained 3h after LPS or saline injection. On day 21, the piglets were killed to collect liver samples. Dietary NAC supplementation attenuated LPS-induced liver histomorphological abnormalities. Compared with the control group, in the LPS-challenged piglets, the activities of alanine aminotransferase and aspartate aminotransferase and the concentrations of H2O2, TNF-α, IL-6 and PGE2 were dramatically increased in the plasma and the activity of superoxide dismutase in the plasma and that of glutathione peroxidase in the liver were significantly decreased. The LPS challenge also increased the concentration of AMP and the ratio of AMP:ATP, but decreased adenylate energy charges and the levels of ATP and ADP. These adverse effects of the LPS challenge were ameliorated by NAC supplementation. Moreover, NAC inhibited the LPS-induced increases in the abundance of liver heat shock protein 70 and NF-κB proteins. In conclusion, these results suggest that dietary NAC supplementation alleviates LPS-induced liver injury by reducing the secretion of pro-inflammatory cytokines, increasing the antioxidative capacity and improving energy metabolism. © 2013 The Authors.
语种:
英文
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仔猪溃疡性结肠炎模型的建立
作者:
陈星;王清静;王蕾;朱惠玲;丁斌鹰;...
期刊:
饲料工业 ,2013年(19):24-27 ISSN:1001-991X
作者机构:
动物营养与饲料科学湖北省重点实验室武汉轻工大学,湖北武汉,430023;[陈星; 丁斌鹰; 侯永清; 王清静; 王蕾; 朱惠玲] 武汉轻工大学
关键词:
仔猪;结肠炎;乙酸
摘要:
试验旨在建立乙酸诱导仔猪溃疡性结肠炎模型。选取18头健康仔猪(杜洛克×长白×大白),随机分成3组:对照组(3头)、三硝基苯磺酸(TNBS)组(6头)和乙酸组(9头),分别注射30 mg/kg BW戊巴比妥钠,待完全麻醉后,对照组仔猪直肠灌注10 ml无菌生理盐水,TNBS组分别灌注25 mg/kg BW(3头)和50 mg/kg BW(3头)TNBS的50%乙醇溶液,乙酸组分别灌注10 ml 5%(3头)和10%的乙酸(6头)。随时观察仔猪状态与粪便情况,灌注3 d或7 d后屠宰,并取结肠组织固定,制作病理切片观察损伤变化,分别进行黏膜损伤评分,以判定造模是否成功。结果表明,采用直肠灌注不同浓度TNBS(25 mg/kg BW和50 mg/kg BW)、5%乙酸,均只出现短暂的稀便,病理切片观察只有轻微炎性症状,与对照组没有显著差异;灌注10%乙酸的6头仔猪,根据粪便性状及结肠肉眼观察均显示明显的结肠炎症状,同时通过组织学观察发现,3 d后出现溃疡灶,并有明显的炎性细胞浸润等炎症反应,而7 d后可见溃疡灶已脱落,炎性细胞浸润明显减少,大量成纤维细胞增生。由此可见,直肠灌注10%乙酸能成功诱导仔猪结肠炎。
语种:
中文
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N-acetylcysteine reduces inflammation in the small intestine by regulating redox, EGF and TLR4 signaling
作者:
Hou, Yongqing* ;Wang, Lei;Yi, Dan;Ding, Binying;Yang, Zhenguo;...
期刊:
Amino Acids ,2013年45(3):513-522 ISSN:0939-4451
通讯作者:
Hou, Yongqing
作者机构:
[Hou, Yongqing; Yang, Zhenguo; Chen, Xing; Wang, Lei; Qiu, Yinsheng; Ding, Binying; Li, Jiao; Yi, Dan] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Wu, Guoyao] China Agr Univ, State Key Lab Anim Nutr, Beijing 100193, Peoples R China.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.
通讯机构:
[Hou, Yongqing] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
关键词:
Aquaporin;Epidermal growth factor;Intestinal mucosa;Lipopolysaccharide;N-acetylcysteine;Toll like receptor
摘要:
This study determined whether N-acetylcysteine (NAC) could affect intestinal redox status, proinflammatory cytokines, epidermal growth factor (EGF), EGF receptor (EGFR), Toll-like receptor-4 (TLR4), and aquaporin-8 in a lipopolysaccharide (LPS)-challenged piglet model. Eighteen piglets (35-day-old) were randomly allocated into one of the three treatments (control, LPS and NAC). The control and LPS groups were fed a basal diet, and the NAC group received the basal diet +500 mg/kg NAC. On days 10, 13, and 20 of the trial, the LPS- and NAC-treated piglets received intraperitoneal administration of LPS (100 μg/kg BW), whereas the control group received the same volume of saline. On days 10 and 20, venous blood samples were obtained at 3 h post LPS or saline injection. On day 21 of the trial, piglets were killed to obtain the intestinal mucosa for analysis. Compared with the control group, LPS challenge reduced (P < 0.05) the activities of superoxide dismutase, catalase, and glutathione peroxidase in jejunal mucosae, while increasing (P < 0.05) the concentrations of malondialdehyde, H2O2, O2 ·- and the ratio of oxidized to reduced glutathione in jejunal mucosae, and concentrations of TNF-α, cortisol, interleukin-6, and prostaglandin E2 in both plasma and intestinal mucosae. These adverse effects of LPS were attenuated (P < 0.05) by NAC supplementation. Moreover, NAC prevented LPS-induced increases in abundances of intestinal HSP70 and NF-κB p65 proteins and TLR4 mRNA. NAC supplementation enhanced plasma EGF concentration and intestinal EGFR mRNA levels. Collectively, these results indicate that dietary NAC supplementation alleviates LPS-induced intestinal inflammation via regulating redox, EGF, and TLR4 signaling. © 2012 Springer-Verlag.
语种:
英文
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Effects of enzyme complex supplementation to a paddy-based diet on performance and nutrient digestibility of meat-type ducks
作者:
Kang, P.;Hou, Y. Q.;Toms, Derek;Yan, N. D.;Ding, B. Y.* ;...
期刊:
Animal Bioscience ,2013年26(2):253-259 ISSN:2765-0189
通讯作者:
Ding, B. Y.
作者机构:
[Hou, Y. Q.; Ding, B. Y.; Yan, N. D.; Kang, P.] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan, Hubei, Peoples R China.;[Toms, Derek] Univ Guelph, Dept Anim & Poultry Sci, Guelph, ON N1G 2W1, Canada.;[Gong, Joshua] Agr & Agri Food Canada, Guelph Food Res Ctr, Guelph, ON, Canada.
通讯机构:
[Ding, B. Y.] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan, Hubei, Peoples R China.
关键词:
Toms, Derek;Gong, Joshua;Xylanase;beta-Glucanase;Cellulase;Paddy;Ducks
摘要:
Paddy rice is rarely used as a feed because of its high fiber content. In this study, two experiments were conducted to study the effects of supplementing an enzyme complex consisting of xylanase, beta-glucanase and cellulase, to paddy-based diets on the performance and nutrient digestibility in meat-type ducks. In the both experiments, meat-type ducks (Cherry Valley) were randomly assigned to four treatments. Treatment 1 was a basal diet of corn-soybean; treatment 2 was a basal diet of corn-paddy-soybean; treatment 3, had enzyme complex added to the corn-paddy-soybean basal diet at levels of 0.5 g/kg diet; and treatment 4, had enzyme complex added to the corn-paddy-soybean diet at levels of 1.0 g/kg diet. The results showed that the enzyme complex increased the ADG, and decreased the ADFI and F/G significantly (p<0.05) in the ducks, and the ADFI for the ducks fed the corn-paddy-soybean diet showed no difference compared to the ducks fed corn-soybean diets at all stages of the experiment (p<0.05). When corn was partially replaced by paddy, the digestibility of CP and NDF was decreased and increased, respectively (p<0.05), and the level of enzyme complex had a significant effect on both CP and NDF digestibility (p<0.05). As for the AME, addition of enzyme complex increased it significantly (p<0.05), but both diet types and levels of enzyme complex had no effect (p>0.05). The outcome of this research indicates that the application of enzyme complex made up of xylanase, beta-glucanase, and cellulase, in the corn-paddy-soybean diet, can improve performance and nutrition digestibility in meat-type ducks. Copyright © 2013 by Asian-Australasian Journal of Animal Sciences.
语种:
英文
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鱼油对脂多糖刺激仔猪肠黏膜能量代谢的影响
作者:
王秀英;刘玉兰;李藏兰;陈逢;朱惠玲;...
期刊:
中国畜牧杂志 ,2013年49(23):22-25 ISSN:0258-7033
作者机构:
1. 武汉轻工大学动物营养与饲料科学湖北省重点实验室;2. 中国农业大学动物科技学院;3. 福建高龙实业有限公司;[李藏兰] 中国农业大学;[丁斌鹰; 侯永清; 李爽; 王秀英; 朱惠玲; 陈逢; 刘玉兰; 石海峰] 武汉轻工大学
关键词:
鱼油;脂多糖;肠黏膜;能量代谢;仔猪
摘要:
试验研究鱼油对脂多糖(LPS)刺激仔猪肠黏膜能量代谢的影响。选用32头(28±3)d(8.91±0.74)kg的杜洛克×长白×大白仔猪,采用2×2因子设计,主因子包括日粮处理(5%鱼油或5%玉米油)和免疫应激(注射LPS或生理盐水),试验期19 d。试验第19天,每日粮组一半的猪注射100μg/kg BW的LPS,另一半注射生理盐水作对照。注射LPS 4 h后屠宰仔猪,取小肠样品待测。结果表明:鱼油对肠黏膜能量代谢的影响与LPS刺激不存在显著的互作关系;LPS刺激导致结肠三磷酸腺苷(ATP)水平显著下降(P<0.05);鱼油显著提高了结肠三磷酸腺苷(ATP)、一磷酸腺苷(AMP)、腺苷酸池(TAN)水平(P<0.05),对结肠AMP/ATP比值有提高的趋势(P<0.10),鱼油也显著降低了结肠能荷(EC)水平(P<0.05);鱼油和LPS刺激对空肠和回肠能量代谢指标均无显著影响。以上研究结果显示,鱼油可以缓解LPS应激引起的仔猪结肠黏膜能量代谢障碍,从而保护肠黏膜屏障。
语种:
中文
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Fish oil attenuates liver injury caused by LPS in weaned pigs associated with inhibition of TLR4 and nucleotide-binding oligomerization domain protein signaling pathways:
作者:
Chen, Feng;Liu, Yulan* ;Zhu, Huiling;Hong, Yu;Wu, Zhifeng;...
期刊:
Innate Immunity ,2013年19(5):504-515 ISSN:1753-4259
通讯作者:
Liu, Yulan
作者机构:
[Liu, Yulan; Wu, Zhifeng; Li, Quan; Hou, Yongqing; Chen, Feng; Hong, Yu; Ding, Binying; Yi, Dan; Zhu, Huiling; Chen, Hongbo] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
通讯机构:
[Liu, Yulan] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
关键词:
Fish oil;lipopolysaccharide;weaned pigs;liver injury;pro-inflammatory cytokines;Toll-like receptor 4;nucleotide-binding oligomerization domain protein
摘要:
This study evaluated whether fish oil exerted a hepatoprotective effect in a LPS-induced liver injury model via regulation of TLR4 and nucleotide-binding oligomerization domain protein (NOD) signaling pathways. Twenty-four piglets were used in a 2 × 2 factorial design, and the main factors included diet (5% corn oil or 5% fish oil) and immunological challenge (LPS or saline). Fish oil resulted in enrichment of eicosapentaenoic acid, docosahexaenoic acid and total (n-3) polyunsaturated fatty acids in liver. Less severe liver injury was observed in pigs fed fish oil, as evidenced by improved serum biochemical parameters and less severe histological liver damage. In addition, higher expression of liver tight junction proteins, and lower hepatocyte proliferation and higher hepatocyte apoptosis were observed in pigs fed fish oil. The improved liver integrity in pigs fed fish oil was concurrent with reduced hepatic mRNA expression of TLR4, myeloid differentiation factor 88, IL-1 receptor-associated kinase 1 and TNF-α receptor-associated factor 6, and NOD1, NOD2 and receptor-interacting serine/threonine-protein kinase 2, as well as reduced hepatic protein expression of NF-κB p65, leading to reduced hepatic pro-inflammatory mediators. These results indicate that fish oil improves liver integrity partially via inhibition of TLR4 and NOD signaling pathways under an inflammatory condition. © 2013 The Author(s).
语种:
英文
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Protective effects of N-acetylcysteine on acetic acid-induced colitis in a porcine model
作者:
Wang, Qingjing;Hou, Yongqing* ;Yi, Dan;Wang, Lei;Ding, Binying;...
期刊:
BMC Gastroenterology ,2013年13(1):1-11 ISSN:1471-230X
通讯作者:
Hou, Yongqing
作者机构:
[Long, Minhui; Liu, Yulan; Hou, Yongqing; Chen, Xing; Wang, Lei; Wang, Qingjing; Ding, Binying; Yi, Dan] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.;[Wu, Guoyao] China Agr Univ, State Key Lab Anim Nutr, Beijing 100193, Peoples R China.
通讯机构:
[Hou, Yongqing] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
关键词:
N-acetylcysteine;Acetic acid;Colon injury;Claudin-1;Epidermal growth factor;Amphiregulin
摘要:
Background: Ulcerative colitis is a chronic inflammatory disease and involves multiple etiological factors. Acetic acid (AA)-induced colitis is a reproducible and simple model, sharing many characteristics with human colitis. N-acetylcysteine (NAC) has been widely used as an antioxidant in vivo and in vitro. NAC can affect several signaling pathways involving in apoptosis, angiogenesis, cell growth and arrest, redox-regulated gene expression, and inflammatory response. Therefore, NAC may not only protect against the direct injurious effects of oxidants, but also beneficially alter inflammatory events in colitis. This study was conducted to investigate whether NAC could alleviate the AA-induced colitis in a porcine model.Methods: Weaned piglets were used to investigate the effects of NAC on AA-induced colitis. Severity of colitis was evaluated by colon histomorphology measurements, histopathology scores, tissue myeloperoxidase activity, as well as concentrations of malondialdehyde and pro-inflammatory mediators in the plasma and colon. The protective role of NAC was assessed by measurements of antioxidant status, growth modulator, cell apoptosis, and tight junction proteins. Abundances of caspase-3 and claudin-1 proteins in colonic mucosae were determined by the Western blot method. Epidermal growth factor receptor, amphiregulin, tumor necrosis factor-alpha (TNF-α), and toll-like receptor 4 (TLR4) mRNA levels in colonic mucosae were quantified using the real-time fluorescent quantitative PCR.Results: Compared with the control group, AA treatment increased (P < 0.05) the histopathology scores, intraepithelial lymphocyte (IEL) numbers and density in the colon, myeloperoxidase activity, the concentrations of malondialdehyde and pro-inflammatory mediators in the plasma and colon, while reducing (P < 0.05) goblet cell numbers and the protein/DNA ratio in the colonic mucosa. These adverse effects of AA were partially ameliorated (P < 0.05) by dietary supplementation with NAC. In addition, NAC prevented the AA-induced increase in caspase-3 protein, while stimulating claudin-1 protein expression in the colonic mucosa. Moreover, NAC enhanced mRNA levels for epidermal growth factor and amphiregulin in the colonic mucosa.Conclusion: Dietary supplementation with NAC can alleviate AA-induced colitis in a porcine model through regulating anti-oxidative responses, cell apoptosis, and EGF gene expression. © 2013 Wang et al.; licensee BioMed Central Ltd.
语种:
英文
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猪源枯草芽孢杆菌的分离鉴定及生物学特性研究
作者:
窦茂鑫;赵迪;王蕾;丁斌鹰;侯永清;...
期刊:
养殖与饲料 ,2012年(4):11-14 ISSN:1671-427X
作者机构:
武汉工业学院动物科学与营养工程学院/动物营养与饲料科学湖北省重点实验室,武汉,430023;[丁斌鹰; 侯永清; 窦茂鑫; 王蕾; 赵迪; 吴涛] 武汉工业学院
关键词:
枯草芽孢杆菌;生物学特性;耐受性;猪粪便;猪微生态饲料添加剂
摘要:
本研究旨在分离、鉴定出经过猪肠道驯化的枯草芽孢杆菌,研究其耐高温、耐酸、耐胆盐、抑菌性等生物学特性,为猪微生态饲料添加剂的研发提供候选菌株。试验以新鲜的猪粪便为材料,通过细菌培养特征、形态观察、生化鉴定、16SrDNA序列分析,分离、鉴定出5株枯草芽孢杆菌;对所鉴定的枯草芽孢杆菌进行高温处理、酸处理和胆盐处理后,比较其耐高温、耐酸、耐胆盐的能力;通过对常见病原菌的抑菌性的测定,比较其抑菌效果。结果表明:5株枯草芽孢杆菌均具有一定的生物学特性,但性能参差不齐,其中BS7菌株整体特性最好。因此该批从猪粪便中分离的枯草芽孢杆菌中BS7菌株具有较好的生物学特性,可以作为猪微生态饲料添加剂的候选菌株。
语种:
中文
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Effects of Oral Administration of Spermine on the Development of Small Intestine and Growth Performance of Weaned Pigs
作者:
Kang, Ping;Wang, Meng;Hou, Yongqing* ;Yin, Yulong;Ding, Binying;...
期刊:
Journal of Animal and Veterinary Advances ,2012年11(15):2782-2787 ISSN:1680-5593
通讯作者:
Hou, Yongqing
作者机构:
[Liu, Yulan; Kang, Ping; Hou, Yongqing; Wang, Lei; Qiu, Yinsheng; Wang, Meng; Yin, Yulong; Ding, Binying; Yi, Dan; Zhu, Huiling] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Yin, Yulong] Chinese Acad Sci, Inst Subtrop Agr, Key Lab Agroecol Proc Subtrop Reg, Changsha 410125, Hunan, Peoples R China.;[Gong, Joshua] Agr & Agri Food Canada, Guelph Food Res Ctr, Guelph, ON N1G 5C9, Canada.
通讯机构:
[Hou, Yongqing] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
关键词:
D-xylose;Growth performance;Intestinal morphology;Intestinal protein/DNA;Piglets;Spermine
摘要:
The effects of spermine on the development of small intestine and growth performance of newly weaned pigs were investigated. Thirty pigs were used and the treatment of spermine was through oral administration for the 1 st 3 days after weaning with the dose of 0, 300, 600, 900 or 1200 umol per pig daily. The administration of spermine improved Average Daily Feed Intake (ADFI) and Average Daily Gam (ADG) in the periods 0-14 (p<0.05) but hadno significant effects on the ratio of Feed to Gain (F/G) and protein/DNA contents of the duodenal or ileal mucosa. The protein/DNA content of the jejunum was significantly increased at the doses of 300 and 600 umol day"1 spermine but was declined at the higher doses. The villus height in the duodenum (p<0.01) and ileum (p<0.05) increased with an increase in spermine doses. The crypt depth was reduced by spermine in the jejunum and the ratio of villus height/crypt depth was increased by spermine in the duodenum, jejunum and ileum (p<0.05). Additionally, the treatment of spermine at tested doses showed no effect on D-xylose absorption by the small intestine. These results indicate that oral administration of spermine for 3 days after weaning increased ADG and ADFI, enhanced the development of the small intestinal mucosa and improved intestinal morphology. © Medwell Journals, 2012.
语种:
英文
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Protective effects of N-acetylcysteine on intestinal functions of piglets challenged with lipopolysaccharide
作者:
Hou, Yongqing* ;Wang, Lei;Zhang, Wei;Yang, Zhenguo;Ding, Binying;...
期刊:
Amino Acids ,2012年43(3):1233-1242 ISSN:0939-4451
通讯作者:
Hou, Yongqing
作者机构:
[Liu, Yulan; Zhang, Wei; Hou, Yongqing; Yang, Zhenguo; Wang, Lei; Qiu, Yinsheng; Ding, Binying; Zhu, Huiling] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.
通讯机构:
[Hou, Yongqing] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
关键词:
Intestinal functions;Lipopolysaccharide;N-acetylcysteine;Piglets
摘要:
The neonatal small intestine is susceptible to damage by endotoxin, but effective methods for prevention and treatment are lacking. N-acetylcysteine (NAC) is a widely used precursor of L-cysteine for animal cells and plays an important role in protecting cells against oxidative stress. This study was conducted with the lipopolysaccharide (LPS)-challenged piglet model to determine the effects of NAC on intestinal function. Eighteen piglets were randomly allocated into control, LPS and LPS + NAC groups. The control and LPS groups were fed a corn- and soybean meal-based diet, and the LPS + NAC group was fed the basal diet +500 mg/kg NAC. On days 10, 13 and 20 of the trial, the LPS and LPS + NAC groups received intraperitoneal administration of LPS (100 lg/kg BW), whereas the control piglets received saline. On day 20 of the trial, D-xylose (0.1 g<kg BW) was orally administrated to all piglets 2 h after LPS or saline injection, and blood samples were collected 1 h thereafter. One hour blood xylose test was used to measure intestinal absorption capacity and mucosal integrity, and diamine oxidase (DAO) was used as a marker of intestinal injury. On day 21 of the trial, pigs were killed to obtain the intestinal mucosa. Compared to the control, LPS challenge reduced (P<0.05) the concentrations of D-xylose (a marker of intestinal absorption) in plasma, activities of DAO in the jejunal mucosa, the ratio of villus height to crypt depth in the jejunal mucosa, RNA/DNA and protein/DNA in the jejunal and ileal mucosae, while increasing (P<0.05) DAO activity in plasma and caspase-3 expression in the intestinal mucosa. The adverse effects of LPS were partially ameliorated (P<0.05) by NAC supplementation. Moreover, NAC prevented the LPS-induced decrease in claudin-1 and occludin expression in the jejunal and ileal mucosae. Collectively, these results indicate that dietary NAC supplementation alleviates the mucosal damage and improves the absorptive function of the small intestine in LPS-challenged piglets. © 2011 Springer-Verlag.
语种:
英文
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Effects of Dietary Silymarin Supplementation on Growth Performance and Oxidative Status in Carassius auratus gibelio
作者:
Yi, Dan;Gu, Lingfang;Ding, Binying;Li, Ming;Hou, Yongqing* ;...
期刊:
Journal of Animal and Veterinary Advances ,2012年11(18):3399-3404 ISSN:1680-5593
通讯作者:
Hou, Yongqing
作者机构:
[Li, Ming; Gu, Lingfang; Hou, Yongqing; Wang, Lei; Ding, Binying; Yi, Dan] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Gong, Joshua] Agr & Agri Food Canada, Guelph Food Res Ctr, Guelph, ON N1G 5C9, Canada.
通讯机构:
[Hou, Yongqing] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
关键词:
Antioxidant;Body composition;Carassius auratus gibelio;Growth performance;Liver;Silymarin
摘要:
This study was conducted to investigate the effects of dietary silymarin supplementation on growth performance and oxidative status in Carassius auratus gibelio. About 270 fishes (C. auratus gibelio) were randomly assigned to the following groups: the control group was fed a basal diet; flavomycin group and silymarin group were fed a basal diet supplemented with 4 mg kg-1 flavomycin and 100 mg kg-1 silymarin, respectively. After 8 weeks feeding, body weight and feed intake were recorded. Blood, liver and muscle sample were also collected for determining the biochemical parameters, oxidative status, protein, RNA and DNA levels. No significant differences in growth performance and body composition of fishes were observed among three groups. However, dietary supplementation with silymarin increased the levels of blood total protein and globulin as compared to flavomycin group. Fishes receiving silymarin exhibited a lower level of malondialdehyde in serum and liver compared to control and flavomycin groups whereas levels of liver and serum superoxide dismutase were not affected. In addition, dietary silymarin increased the ratio of protein to RNA of liver whereas did not affect the ratio of RNA to DNA in muscle and liver. Collectively, these data suggested that dietary supplementation of 100 mg kg-1 silymarin increased the protein synthesis and potentially improved the antioxidative capacity in C. auratus gibelio. © Medwell Journals, 2012.
语种:
英文
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Diet Supplementation of Silymarin Increased the Antioxidantive Capacity in Cumene Hydroperoxide-Challenged Ducks
作者:
Yi, Dan;Wang, Cong;Sun, Danfeng;Hou, Yongqing;Ding, Binying* ;...
期刊:
Journal of Animal and Veterinary Advances ,2012年11(16):2986-2994 ISSN:1680-5593
通讯作者:
Ding, Binying
作者机构:
[Wang, Cong; Hou, Yongqing; Sun, Danfeng; Wang, Lei; Ding, Binying; Yi, Dan] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Gong, Joshua] Agr & Agri Food Canada, Guelph Food Res Ctr, Guelph, ON, Canada.
通讯机构:
[Ding, Binying] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
关键词:
Silymarin;antioxidantive capacity;cumene hydroperoxide;liver;intestinal mucosa;ducks
摘要:
This study was conducted to investigate the efficacy of Silymarin (SIL) as antioxidant against Cumene Hhydroperoxide (CH) induced oxidative damage in liver and intestinal mucosa of ducks. One hundred and sixty male Cherry Valley ducks (28 days old) were randomly allotted to control, CH, CH + 100 SIL (100 mg kg-1 diet) and CH + 200 SIL (200 mg kg-1 diet) groups. At the age of 42 days, ducks in CH, CH + 100 SIL and CH + 200 SIL groups received intraperitoneal administration of CH (30 mg kg-1 BW) whereas ducks in the control group received the same volume of sterile saline for 5 consecutive days. At the age of 50 days, the blood samples were collected and ducks were killed to obtain liver and intestine for analysis. Compared with the control group, CH challenge increased the levels of plasma Glutamic-Pyruvic Transaminase (GPT), Alkaline Phosphatase (AKP), hepatic and Mucosal Malondialdehyde (MDA) (p<0.05) but reduced the levels of Superoxide Dismutase (SOD), Catalase (CAT) and Glutathione S-Transferase (GST) in liver (p<0.05). CH-treated ducks exhibited a higher crypt depth (p<0.05) and a lower concentration of protein, RNA, DNA and the activities of sucrase and maltase in intestine mucosa (p<0.05). These adverse effects of CH were attenuated (p<0.05) by supplementation of SIL, particularly at the dosage of 200 mg kg-1 diet. These data suggest that dietary supplement with 200 mg kg-1 SIL alleviates hepatic oxidative injury and improves intestinal absorptive capacity in CH-challenged ducks. © Medwell Journals, 2012.
语种:
英文
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