Asparagine reduces the mRNA expression of muscle atrophy markers via regulating protein kinase B (Akt), AMP-activated protein kinase α, toll-like receptor 4 and nucleotide-binding oligomerisation domain protein signalling in weaning piglets after lipopolysaccharide challenge
作者：
Wang, Xiuying;Liu, Yulan* ;Wang, Shuhui;Pi, Dingan;Leng, Weibo;...
期刊：
British Journal of Nutrition ,2016年116(7):1188-1198 ISSN：0007-1145
通讯作者：
Liu, Yulan
作者机构：
[Zhang, Jing; Liu, Yulan; Leng, Weibo; Shi, Haifeng; Li, Shuang; Wang, Shuhui; Pi, Dingan; Wang, Xiuying; Zhu, Huiling] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Odle, Jack; Lin, Xi] North Carolina State Univ, Dept Anim Sci, Lab Dev Nutr, Raleigh, NC 27695 USA.
通讯机构：
[Liu, Yulan] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
关键词：
Asparagine;Lipopolysaccharides;Muscle atrophy;Pro-inflammatory cytokines
摘要：
Pro-inflammatory cytokines are critical in mechanisms of muscle atrophy. In addition, asparagine (Asn) is necessary for protein synthesis in mammalian cells. We hypothesised that Asn could attenuate lipopolysaccharide (LPS)-induced muscle atrophy in a piglet model. Piglets were allotted to four treatments (non-challenged control, LPS-challenged control, LPS+0·5 % Asn and LPS+1·0 % Asn). On day 21, the piglets were injected with LPS or saline. At 4 h post injection, piglet blood and muscle samples were collected. Asn increased protein and RNA content in muscles, and decreased mRNA expression of muscle atrophy F-box (MAFbx) and muscle RING finger 1 (MuRF1). However, Asn had no effect on the protein abundance of MAFbx and MuRF1. In addition, Asn decreased muscle AMP-activated protein kinase (AMPK) α phosphorylation, but increased muscle protein kinase B (Akt) and Forkhead Box O (FOXO) 1 phosphorylation. Moreover, Asn decreased the concentrations of TNF-α, cortisol and glucagon in plasma, and TNF-α mRNA expression in muscles. Finally, Asn decreased mRNA abundance of muscle toll-like receptor (TLR) 4 and nucleotide-binding oligomerisation domain protein (NOD) signalling-related genes, and regulated their negative regulators. The beneficial effects of Asn on muscle atrophy may be associated with the following: (1) inhibiting muscle protein degradation via activating Akt and inactivating AMPKα and FOXO1; and (2) decreasing the expression of muscle pro-inflammatory cytokines via inhibiting TLR4 and NOD signalling pathways by modulation of their negative regulators. © The Authors 2016.
语种：
英文
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LPS通过AKT/FOXO1信号通路诱导C2C12肌管细胞MuRF1基因转录
作者：
刘妍;梁辉煌;刘玉兰;唐中林;汪文俊;...
期刊：
畜牧兽医学报 ,2016年47(2):374-380 ISSN：0366-6964
作者机构：
武汉轻工大学, 动物营养与饲料科学湖北省重点实验室, 武汉, 430023;中国农业科学院北京畜牧兽医研究所, 北京, 100193;中南民族大学生命科学学院, 武汉, 430074;[刘妍; 梁辉煌; 刘玉兰; 张晶] 武汉轻工大学, 动物营养与饲料科学湖北省重点实验室, 武汉, 430023;[唐中林] 中国农业科学院北京畜牧兽医研究所, 北京, 100193
会议名称：
中国畜牧兽医学会动物营养学分会第十二次动物营养学术研讨会
会议时间：
2016-10-21
会议地点：
中国湖北武汉
关键词：
脂多糖;C2C12肌管细胞;肌肉蛋白降解;MuRF1基因;MAFbx基因
摘要：
以C2C12肌管细胞为试验材料,探讨脂多糖(LPS)诱导的炎症反应对肌管蛋白降解相关基因及信号通路的影响。选取不同质量浓度(10、100、1 000ng?mL-1)的LPS分别刺激C2C12肌管细胞30min和3h,通过RTqPCR检测炎症因子TNF-α和IL-6的基因转录,确定LPS最佳刺激浓度以建立细胞炎症模型。用最佳浓度LPS分别刺激C2C12肌管细胞0min、30min、1h、3h、6h、12h、24h,RT-qPCR检测MAFbx基因和MuRF基因在不同时间点的转录量变化。此外,1 000ng?mL-1 LPS刺激C2C12肌管细胞12h后,Western blot检测AKT/FOXO1、mTOR和P38信号通路相关蛋白质的表达情况。结果表明:LPS刺激C2C12肌管细胞的最佳浓度为1 000ng?mL-1,在作用30min和3h时均能显著上调TNF-α、IL-6的基因转录,而在刺激12和24h时MuRF1、IL-1β、IL-6和TLR4的基因转录显著上调。此外,1 000ng?mL-1 LPS刺激C2C12肌管细胞12h时,仅AKT和FOXO1蛋白磷酸化水平明显降低。基于以上结果,推测LPS通过调控AKT/FOXO1信号通路诱导C2C12肌管细胞MuRF1转录。
语种：
中文
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Fish oil enhances intestinal barrier function and inhibits corticotropin-releasing hormone/corticotropin-releasing hormone receptor 1 signalling pathway in weaned pigs after lipopolysaccharide challenge
作者：
Zhu, Huiling;Liu, Yulan* ;Chen, Shaokui;Wang, Xiuying;Pi, Dingan;...
期刊：
British Journal of Nutrition ,2016年115(11):1947-1957 ISSN：0007-1145
通讯作者：
Liu, Yulan
作者机构：
[Zhang, Jing; Liu, Yulan; Leng, Weibo; Chen, Shaokui; Kang, Ping; Chen, Feng; Pi, Dingan; Wang, Xiuying; Zhu, Huiling] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
通讯机构：
[Liu, Yulan] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
关键词：
Lipopolysaccharides;Fish oil;Intestinal barrier function;Weaned pigs
摘要：
Stress induces injury in intestinal barrier function in piglets. Long-chain n-3 PUFA have been shown to exhibit potential immunomodulatory and barrier protective effects in animal models and clinical trials. In addition, corticotropin-releasing hormone (CRH)/CRH receptor (CRHR) signalling pathways play an important role in stress-induced alterations of intestinal barrier function. We hypothesised that fish oil could affect intestinal barrier function and CRH/CRHR signalling pathways. In total, thirty-two weaned pigs were allocated to one of four treatments. The experiment consisted of a 2×2 factorial design, and the main factors included immunological challenge (saline or lipopolysaccharide (LPS)) and diet (5 % maize oil or 5 % fish oil). On d 19 of the trial, piglets were treated with saline or LPS. At 4 h after injection, all pigs were killed, and the mesenteric lymph nodes (MLN), liver, spleen and intestinal samples were collected. Fish oil decreased bacterial translocation incidence and the number of translocated micro-organisms in the MLN. Fish oil increased intestinal claudin-1 protein relative concentration and villus height, as well as improved the intestinal morphology. In addition, fish oil supplementation increased intestinal intraepithelial lymphocyte number and prevented elevations in intestinal mast cell and neutrophil numbers induced by LPS challenge. Moreover, fish oil tended to decrease the mRNA expression of intestinal CRHR1, CRH and glucocorticoid receptors. These results suggest that fish oil supplementation improves intestinal barrier function and inhibits CRH/CRHR1 signalling pathway and mast cell tissue density. © The Authors 2016.
语种：
英文
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鱼油提高LPS应激仔猪小肠屏障功能、抑制CRH/CRHR1信号通路
作者：
朱惠玲;刘玉兰;陈少魁;王秀英;皮定安;...
作者机构：
[朱惠玲; 刘玉兰; 陈少魁; 王秀英; 皮定安; 冷炜博; 陈逢; 张晶; 康萍] 武汉轻工大学动物营养与饲料科学湖北省重点实验室动物营养与饲料安全湖北省协同创新中心
会议名称：
中国畜牧兽医学会动物营养学分会第十二次动物营养学术研讨会
会议时间：
2016-10-21
会议地点：
中国湖北武汉
会议论文集名称：
中国畜牧兽医学会动物营养学分会第十二次动物营养学术研讨会论文集
关键词：
脂多糖;鱼油;小肠屏障功能;断奶仔猪
摘要：
应激可引起仔猪小肠屏障功能障碍。大量研究表明,糖皮质激素释放激素(CRH)/糖皮质激素释放激素受体1(CRHR1)信号通路在应激诱导小肠屏障功能障碍中发挥重要作用。临床试验及动物模型研究表明,n-3多不饱和脂肪酸具有免疫调节及屏障保护功能。本试验用脂多糖(LPS)刺激建立免疫应激模型,研究鱼油对LPS应激仔猪小肠屏障功能及CRH/CRHR1信号通路的影响,旨在为缓解仔猪免疫应激提供理论依据。选用3
语种：
中文
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Effects of full-fat rice bran inclusion in diets on growth performance and meat quality of Sichuan goose
作者：
Sun, W.;Kang, P.* ;Xie, M.;Hou, S. S.;Wu, T.;...
期刊：
British Poultry Science ,2016年57(5):655-662 ISSN：0007-1668
通讯作者：
Kang, P.
作者机构：
[Liu, Y. L.; Hou, Y. Q.; Wu, T.; Kang, P.; Sun, W.; Mei, H. M.; Wu, L. Y.] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Sun, W.; Xie, M.; Hou, S. S.] Chinese Acad Agr Sci, Inst Anim Sci, Beijing, Peoples R China.
通讯机构：
[Kang, P.] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
摘要：
This trial was conducted to study the effect of full-fat rice bran inclusion in diets on growth performance, carcass and meat quality and fatty acid composition in Sichuan goose. A total of 204 Sichuan white male geese (28-d-old, 984 ± 15 g) were used in the 42-d assay. Full-fat rice bran inclusion in diets was 0%, 6%, 12% and 18%, respectively. On d 70, two geese from each pen were randomly selected and killed for measuring the carcass and meat quality and the meat fatty acid composition. The results showed that full-fat rice bran inclusion had no effect on average daily gain during 28–56 d, but rice bran inclusion at 18% increased average daily gain during 57–70 d. In addition, the full-fat rice bran supplementation decreased the subcutaneous fat yield, and the inclusion of full-fat rice bran in amounts of 12% and 18% decreased the half-eviscerated carcass yield, eviscerated carcass yield and crude fat content in goose meat. Moreover, full-fat rice bran supplementation had no effect on the content of total saturated fatty acid (SFA), but decreased the content of total monounsaturated fatty acid (MUFA). The inclusion of full-fat rice bran in amounts of 12–18% increased the content of total polyunsaturated fatty acid (PUFA) and total n-6 in goose meat and in the amount of 18% increased n-3 fatty acids content in goose meat. The results indicated that the rice bran inclusion had a positive effect in geese by stimulating growth performance and improving meat quality and fatty acid composition of goose meat. © 2016 British Poultry Science Ltd.
语种：
英文
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Glycine enhances muscle protein mass associated with maintaining Akt-mTOR-FOXO1 signaling and suppressing TLR4 and NOD2 signaling in piglets challenged with LPS
作者：
Liu, Yulan* ;Wang, Xiuying;Wu, Huanting;Chen, Shaokui;Zhu, Huiling;...
期刊：
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY ,2016年311(2):R365-R373 ISSN：0363-6119
通讯作者：
Liu, Yulan
作者机构：
[Wu, Huanting; Zhang, Guolong; Zhang, Jing; Hu, Chien-An Andy; Liu, Yulan; Chen, Shaokui; Hou, Yongqing; Wang, Xiuying; Zhu, Huiling] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Peoples R China.;[Hu, Chien-An Andy] Univ New Mexico, Sch Med, Dept Biochem & Mol Biol, Albuquerque, NM 87131 USA.;[Zhang, Guolong] Oklahoma State Univ, Dept Anim Sci, Stillwater, OK 74078 USA.
通讯机构：
[Liu, Yulan] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Peoples R China.
关键词：
glycine;lipopolysaccharide;muscle;pro-inflammatory cytokines
摘要：
Pro-inflammatory cytokines play a critical role in the pathophysiology of muscle atrophy. We hypothesized that glycine exerted an anti-inflammatory effect and alleviated lipopolysaccharide (LPS)-induced muscle atrophy in piglets. Pigs were assigned to four treatments including the following: 1) nonchallenged control, 2) LPS-challenged control, 3) LPS+1.0% glycine, and 4) LPS+2.0% glycine. After receiving the control, 1.0 or 2.0% glycine-supplemented diets, piglets were treated with either saline or LPS. At 4 h after treatment with saline or LPS, blood and muscle samples were harvested. We found that 1.0 or 2.0% glycine increased protein/DNA ratio, protein content, and RNA/DNA ratio in gastrocnemius or longissimus dorsi (LD) muscles. Glycine also resulted in decreased mRNA expression of muscle atrophy F-box (MAFbx) and muscle RING finger 1 (MuRF1) in gastrocnemius muscle. In addition, glycine restored the phosphorylation of Akt, mammalian target of rapamycin (mTOR), eukaryotic initiation factor 4E binding protein 1 (4E-BP1), and Forkhead Box O 1 (FOXO1) in gastrocnemius or LD muscles. Furthermore, glycine resulted in decreased plasma tumor necrosis factor-α (TNF-α) concentration and muscle TNF-α mRNA abundance. Moreover, glycine resulted in decreased mRNA expresson of Toll-like receptor 4 (TLR4), nucleotide-binding oligomerization domain protein 2 (NOD2), and their respective downstream molecules in gastrocnemius or LD muscles. These results indicate glycine enhances muscle protein mass under an inflammatory condition. The beneficial roles of glycine on the muscle are closely associated with maintaining Akt-mTOR-FOXO1 signaling and suppressing the activation of TLR4 and/or NOD2 signaling pathways. © 2016 the American Physiological Society.
语种：
英文
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天冬酰胺对脂多糖诱导的仔猪肌肉蛋白质合成和降解的调控作用
作者：
王秀英;皮定安;冷炜博;朱惠玲;石海峰;...
作者机构：
[王秀英; 皮定安; 冷炜博; 朱惠玲; 石海峰; 李爽; 刘玉兰] 武汉轻工大学动物营养与饲料科学湖北省重点实验室
会议名称：
中国畜牧兽医学会动物营养学分会第十二次动物营养学术研讨会
会议时间：
2016-10-21
会议地点：
中国湖北武汉
关键词：
天冬酰胺;脂多糖;仔猪;肌肉
摘要：
本试验旨在研究天冬酰胺(Asn)对脂多糖(LPS)刺激仔猪肌肉蛋白质降解的调控作用,并从AMP激活蛋白激酶(AMPK)、蛋白激酶B(Akt)/叉头转录因子(FOXO)、Toll样受体4(TLR4)和核苷酸结合寡聚域受体(NOD)信号通路的角度探讨其机制。选用24头断奶仔猪,分为4个处理组:1)对照组(基础饲粮);2)LPS组(基础饲粮+LPS);3)0.5%Asn组(基础饲粮+0.5%Asn+LP
语种：
中文
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谷氨酸对仔猪肝脏能量代谢及关键酶和相关调节因子mRNA表达的影响
作者：
汪龙梅;王秀英;吴欢听;王海波;陈少魁;...
期刊：
中国畜牧杂志 ,2016年52(23):38-43 ISSN：0258-7033
作者机构：
[汪龙梅; 王秀英; 吴欢听; 王海波; 陈少魁; 朱惠玲; 刘玉兰] 武汉轻工大学动物营养与饲料科学湖北省重点实验室
会议名称：
中国畜牧兽医学会动物营养学分会第十二次动物营养学术研讨会
会议时间：
2016-10-21
会议地点：
中国湖北武汉
关键词：
谷氨酸;脂多糖;肝脏;能量代谢;仔猪
摘要：
本试验旨在研究谷氨酸(Glu)对脂多糖(LPS)刺激仔猪肝脏能量代谢、能量代谢关键酶和相关调节因子mRNA表达的影响。选用24头(28土3)d日龄健康三元杂交断奶仔猪,随机分为4个处理组,每个处理6个重复,每个重复1头猪。4个处理组分别为:1)对照组(基础饲粮);2)LPS组(基础饲粮+LPS);3)1.0%Glu组(基础饲粮+1.0%Glu+LPS);4)2.0%Glu组(基础饲粮+2.0%Gl
语种：
中文
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三乳酸甘油酯对冷应激肉鸡肠道抗氧化能力和能量代谢状况的影响
作者：
廖满;段瑞;谢佳倩;周建;杨书慧;...
期刊：
中国畜牧杂志 ,2016年(13):47-54 ISSN：0258-7033
作者机构：
动物营养与饲料安全湖北省协同创新中心动物营养与饲料科学湖北省重点实验室武汉轻工大学动物科学与营养工程学院
关键词：
乳酸甘油酯;冷应激;抗氧化;能量代谢;肉鸡
摘要：
本试验研究三乳酸甘油酯对冷应激肉鸡肠道的抗氧化能力及能量代谢状况的影响。试验选用150只1日龄Cobb肉鸡,随机分为3个处理组:对照组、冷应激组、乳酸甘油酯组,分别饲喂基础日粮、基础日粮、基础日粮+500mg/kg三乳酸甘油酯,每组5个重复,每个重复10只鸡。试验第1周各组温度控制在32~34℃,第2周控制在30~32℃,试验第3~6周冷应激组、乳酸甘油酯组控制在(10±2)℃,而对照组维持在(26±2)℃,湿度50%~60%。结果表明:与对照组相比,冷应激处理降低了肉鸡生长性能、十二指肠过氧化氢酶(CAT)、空肠超氧化物歧化酶(SOD)、CAT及谷胱甘肽过氧化物酶(GSH-Px)的活力(P<0.05),同时也降低了十二指肠的三磷酸腺苷(ATP)、腺苷酸池(TAN)和能荷(EC)含量,回肠的ATP、二磷酸腺苷(ADP)和EC含量及空肠血红素加氧酶-1(HMOX-1)m RNA相对表达量(P<0.05),但提高了十二指肠和回肠AMP/ATP比值,同时也提高了十二指肠、空肠、回肠AMP依赖的蛋白激酶-α1(AMPK-α1)m RNA相对表达量及十二指肠低氧诱导因子-1(HIF-1)m RNA相对表达量(P<0.05);与冷应激组相比,日粮中添加500 mg/kg三乳酸甘油酯对冷应激肉鸡生长性能并无影响,但提高了冷应激肉鸡肠道SOD活力、十二指肠CAT和空肠GSH-Px活力、十二指肠和回肠ATP、EC含量、十二指肠和回肠血红素加氧酶-1(HMOX-1)m RNA及空肠HIF-1、COXⅢm RNA相对表达量(P<0.05),同时降低了十二指肠和回肠AMP/ATP比值,十二指肠、空肠及回肠AMPK-α1m RNA相对表达量、十二指肠和回肠HIF-1 m RNA相对表达量及空肠PGC-1αm RNA相对表达量(P<0.05);本试验结果表明,三乳酸甘油酯可提高冷应激肉鸡的肠道抗氧化能力、改善肠道的能量代谢状况,这可能与三乳酸甘油酯调控HIF-1/HMOX-1和AMPK信号通路有关。
语种：
中文
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谷氨酸通过TLR4、NOD和mTOR信号通路对LPS诱导的断奶仔猪肠道损伤的调控作用
作者：
王秀英;吴欢听;朱惠玲;刘玉兰
作者机构：
[王秀英; 吴欢听; 朱惠玲; 刘玉兰] 武汉轻工大学动物营养与饲料科学湖北省重点实验室
会议名称：
中国畜牧兽医学会动物营养学分会第十二次动物营养学术研讨会
会议时间：
2016-10-21
会议地点：
中国湖北武汉
关键词：
谷氨酸;仔猪;肠道
摘要：
本试验旨在研究谷氨酸(Glu)对脂多糖(LPS)诱导的仔猪肠道损伤的保护作用,并从哺乳动物雷帕霉素靶蛋白(mTOR)、Toll样受体4(TLR4)和核苷酸结合寡聚域受体(NOD)信号通路的角度探讨其作用机制。选择24头断奶仔猪[杜×长×大,平均体重(7.02±0.21)kg],分为4个处理组:1)对照组;2)LPS组;3)LPS+1.0%Glu组;4)LPS+2.0%Glu组。试验期为28天。在正
语种：
中文
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全脂米糠部分替代玉米对四川白鹅生产性能及肉品质的影响
作者：
孙伟;康萍;谢明;侯水生;吴涛;...
作者机构：
[孙伟; 康萍; 谢明; 侯水生; 吴涛; 梅慧敏; 刘玉兰; 侯永清; 吴灵英] 武汉轻工大学动物科学与营养工程学院;[孙伟; 康萍; 谢明; 侯水生; 吴涛; 梅慧敏; 刘玉兰; 侯永清; 吴灵英] 中国农业科学院北京畜牧兽医研究所
会议名称：
中国畜牧兽医学会动物营养学分会第十二次动物营养学术研讨会
会议时间：
2016-10-21
会议地点：
中国湖北武汉
关键词：
全脂米糠;鹅;胴体和肉品质;脂肪酸组成
摘要：
本试验的目的是研究全脂米糠部分替代玉米后对四川白鹅生产性能及肌肉品质和脂肪酸组成的影响。204只四川白鹅[公鹅,28日龄,(984±15)g]随机分配到四个处理组,进行为期42天的饲养试验。其全脂米糠的添加量分别为0%、6%、12%和18%。每个处理6个重复,每个重复8~9只鹅。在试验第43天,每个重复随机挑选2只鹅,屠宰取胸肌以备分析。研究结果显示,全脂米糠部分替代玉米后对28~56日龄的白鹅日
语种：
中文
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Fatty acids, inflammation and intestinal health in pigs
作者：
Liu, Yulan*
期刊：
畜牧与生物技术杂志(英文版) ,2015年6(1):321-329 ISSN：1674-9782
通讯作者：
Liu, Yulan
作者机构：
[Liu, Yulan; Liu, YL] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
通讯机构：
[Liu, Yulan] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
关键词：
Fatty acids;Inflammation;Intestine;Pigs
摘要：
The intestine is not only critical for nutrient digestion and absorption, but also is the largest immune organ in the body. However, in pig production, inflammation induced by numerous factors, such as pathogen infection and stresses (e.g., weaning), results in intestinal mucosal injury and dysfunction, and consequently results in poor growth of pigs. Dietary fatty acids not only play critical roles in energy homeostasis and cellular membrane composition, but also exert potent effects on intestinal development, immune function, and inflammatory response. Recent studies support potential therapeutic roles for specific fatty acids (short chain and medium chain fatty acids and long chain polyunsaturated fatty acids) in intestinal inflammation of pigs. Results of these new lines of work indicate trophic and cytoprotective effects of fatty acids on intestinal integrity in pigs. In this article, we review the effect of inflammation on intestinal structure and function, and the role of specific fatty acids on intestinal health of pigs, especially under inflammatory conditions.
语种：
英文
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Asparagine attenuates hepatic injury caused by lipopolysaccharide in weaned piglets associated with modulation of Toll-like receptor 4 and nucleotide-binding oligomerisation domain protein signalling and their negative regulators
作者：
Wu, Huanting;Liu, Yulan* ;Pi, Dingan;Leng, Weibo;Zhu, Huiling;...
期刊：
British Journal of Nutrition ,2015年114(2):189-201 ISSN：0007-1145
通讯作者：
Liu, Yulan
作者机构：
[Wu, Huanting; Liu, Yulan; Leng, Weibo; Hou, Yongqing; Li, Shuang; Shi, Haifeng; Pi, Dingan; Wang, Xiuying; Zhu, Huiling] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Peoples R China.
通讯机构：
[Liu, Yulan] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Peoples R China.
关键词：
Asparagine;Hepatic injury;Weaned piglets;Lipopolysaccharide;Toll-like receptor 4;Nucleotide-binding oligomerisation domain protein
摘要：
Pro-inflammatory cytokines play a key role in many models of hepatic damage. In addition, asparagine (Asn) plays an important role in immune function. We aimed to investigate whether Asn could attenuate lipopolysaccharide (LPS)-induced liver damage. Forty-eight castrated barrows were allotted to four groups including: (1) non-challenged control; (2) LPS-challenged control; (3) LPS+0·5 % Asn; and (4) LPS+1·0 % Asn. After 19 d feeding with control, 0·5 or 1·0 % Asn diets, pigs were injected with LPS or saline. Blood and liver samples were obtained at 4 h (early stage) and 24 h (late stage) post-injection. Asn alleviated liver injury, indicated by reduced serum aspartate aminotransferase and alkaline phosphatase activities linearly and quadratically; it increased claudin-1 protein expression linearly and quadratically at 24 h, and less severe liver morphological impairment at 4 or 24 h. In addition, Asn decreased mRNA expression of TNF-α and heat shock protein 70 (HSP70) linearly and quadratically at 4 h; it increased TNF-α mRNA expression, and HSP70 protein expression linearly and quadratically at 24 h. Moreover, Asn increased inducible NO synthase activity linearly and quadratically. Finally, Asn down-regulated the mRNA expression of Toll-like receptor 4 (TLR4) signalling molecules (TLR4, IL-1 receptor-associated kinase 1 (IRAK1), TNF-α receptor-associated factor 6), nucleotide-binding oligomerisation domain protein (NOD) signalling molecules (NOD1, NOD2 and their adaptor molecule receptor-interacting serine/threonine-protein kinase 2 (RIPK2)), and NF-κB p65 linearly or quadratically at 4 h. Oppositely, Asn up-regulated mRNA expressions of TLR4 and NOD signalling molecules (TLR4, myeloid differentiation factor 88, IRAK1, NOD2 and RIPK2), and their negative regulators (radioprotective 105, single Ig IL-1R-related molecule, Erbb2 interacting protein and centaurin β1) linearly or quadratically at 24 h. These results indicate that, in early and late stages of LPS challenge, Asn improves liver integrity and exerts different regulatory effects on mRNA expression of TLR4 and NOD signalling molecules. © The Authors 2015.
语种：
英文
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Analysis of MicroRNA Expression Profiles in Weaned Pig Skeletal Muscle after Lipopolysaccharide Challenge
作者：
Zhang, Jing;Fu, Shu-Lin;Liu, Yan;Liu, Yu-Lan* ;Wang, Wen-Jun
期刊：
International Journal of Molecular Sciences ,2015年16(9):22438-22455 ISSN：1422-0067
通讯作者：
Liu, Yu-Lan
作者机构：
[Liu, Yan; Zhang, Jing; Liu, Yu-Lan] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Fu, Shu-Lin] Wuhan Acad Agr Sci & Technol, Wuhan Inst Anim Husb & Vet Sci, Wuhan 430208, Peoples R China.;[Liu, Yan; Wang, Wen-Jun] South Cent Univ Nationalities, Coll Life Sci, Wuhan 430074, Peoples R China.
通讯机构：
[Liu, Yu-Lan] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
关键词：
LPS;microRNA;pig;skeletal muscle wasting;small RNA sequencing
摘要：
MicroRNAs (miRNAs) constitute a class of non-coding RNAs that play a crucial regulatory role in skeletal muscle development and disease. Several acute inflammation conditions including sepsis and cancer are characterized by a loss of skeletal muscle due primarily to excessive muscle catabolism. As a well-known inducer of acute inflammation, a lipopolysaccharide (LPS) challenge can cause serious skeletal muscle wasting. However, knowledge of the role of miRNAs in the course of inflammatory muscle catabolism is still very limited. In this study, RNA extracted from the skeletal muscle of pigs injected with LPS or saline was subjected to small RNA deep sequencing. We identified 304 conserved and 114 novel candidate miRNAs in the pig. Of these, four were significantly increased in the LPS-challenged samples and five were decreased. The expression of five miRNAs (ssc-miR-146a-5p, ssc-miR-221-5p, ssc-miR-148b-3p, ssc-miR-215 and ssc-miR-192) were selected for validation by quantitative polymerase chain reaction (qPCR), which found that ssc-miR-146a-5p and ssc-miR-221-5p were significantly upregulated in LPS-challenged pig skeletal muscle. Moreover, we treated mouse C2C12 myotubes with 1000 ng/mL LPS as an acute inflammation cell model. Expression of TNF-α, IL-6, muscle atrophy F-box (MAFbx) and muscle RING finger 1 (MuRF1) mRNA was strongly induced by LPS. Importantly, miR-146a-5p and miR-221-5p also showed markedly increased expression in LPS-treated C2C12 myotubes, suggesting the two miRNAs may be involved in muscle catabolism systems in response to acute inflammation caused by a LPS challenge. To our knowledge, this study is the first to examine miRNA expression profiles in weaned pig skeletal muscle challenged with LPS, and furthers our understanding of miRNA function in the regulation of inflammatory muscle catabolism. © 2015 by the authors; licensee MDPI, Basel, Switzerland.
语种：
英文
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The effect of aspartate on the energy metabolism in the liver of weanling pigs challenged with lipopolysaccharide
作者：
Kang, Ping;Liu, Yulan* ;Zhu, Huiling;Li, Shuang;Shi, Haifeng;...
期刊：
European Journal of Nutrition ,2015年54(4):581-588 ISSN：1436-6207
通讯作者：
Liu, Yulan
作者机构：
[Liu, Yulan; Pi, Dinan; Leng, Weibo; Kang, Ping; Li, Shuang; Shi, Haifeng; Hou, Yongqing; Chen, Feng; Yi, Dan; Zhu, Huiling] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
通讯机构：
[Liu, Yulan] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
关键词：
Aspartate;Energy status;Liver;Piglets;Lipopolysaccharide
摘要：
Purpose: This study was conducted to investigate whether aspartate (Asp) could improve liver energy status in the lipopolysaccharide (LPS)-challenged pigs. Methods: Twenty-four weaned pigs were assigned to four treatments: (1) nonchallenged control (control diet and saline-treated); (2) LPS-challenged control (the same control diet and LPS-challenged); (3) LPS + 0.5 % Asp treatment (0.5 % Asp diet and LPS-challenged); and (4) LPS + 1.0 % Asp treatment (a 1.0 % Asp diet and LPS-challenged). On d 19, the pigs were injected intraperitoneally with Escherichia coli LPS at 100 μg/kg body weight, and the same volume of 0.9 % NaCl solution, respectively. All pigs were slaughtered at 24 h after LPS or saline injection, and the liver was collected for further analysis. Results: Dietary supplementation with Asp improved liver energy status evidenced by the increased ATP concentration and adenylate energy charges, and the decreased AMP concentration and AMP/ATP ratio (p < 0.05). Asp supplementation increased the mRNA expression of key enzymes in hepatic glycolysis and tricarboxylic acid (TCA) cycle, including pyruvate kinase and citrate synthase (p < 0.05), and had a tendency to increase hepatic pyruvate dehydrogenase and isocitrate dehydrogenase β mRNA expression (p < 0.10). In addition, Asp increased the mRNA expressions of hepatic AMP-activated protein kinase (AMPK) α1, AMPKα2, silent information regulator (Sirt1), and proliferator-activated receptor-γ coactivator 1α (PGC1α) (p < 0.05). Moreover, Asp increased AMPKα phosphorylation (p < 0.05). Conclusions: These results indicated that dietary supplementation of Asp could improve energy status in LPS-injured liver, which might result from motivating the metabolism pathway of TCA cycle and glycolysis and stimulating the AMPK signaling pathway. © 2014, Springer-Verlag Berlin Heidelberg.
语种：
英文
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L-Glutamine enhances enterocyte growth via activation of the mTOR signaling pathway independently of AMPK
作者：
Yi, Dan;Hou, Yongqing* ;Wang, Lei;Ouyang, Wanjin;Long, Minhui;...
期刊：
Amino Acids ,2015年47(1):65-78 ISSN：0939-4451
通讯作者：
Hou, Yongqing
作者机构：
[Ouyang, Wanjin; Long, Minhui; Liu, Yulan; Hou, Yongqing; Wang, Lei; Wu, Guoyao; Zhao, Di; Ding, Binying; Yi, Dan] Wuhan Polytechn Univ, Hubei Key Lab Anim Nutr & Feed Sci, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Peoples R China.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.
通讯机构：
[Hou, Yongqing] W;Wuhan Polytechn Univ, Hubei Key Lab Anim Nutr & Feed Sci, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Peoples R China.
关键词：
AMPK;IPEC-1;l-Glutamine;mTOR
摘要：
Neonates (including human infants) require l-glutamine (Gln) for optimal intestinal health. This study tested the hypothesis that Gln enhances enterocyte growth via both mammalian target of rapamycin (mTOR) and AMP-activated kinase (AMPK) signaling pathways. Intestinal porcine epithelial cells (IPEC-1) were cultured for 3 days in Gln-free Dulbecco's modified Eagle medium containing 0 or 2 mM Gln. To determine the role of mTOR and AMPK on cell growth, additional experiments were conducted where medium contained 2 mM Gln and 10 nM rapamycin (Rap, an inhibitor of mTOR) or 1 μM compound C (an inhibitor of AMPK). IPEC-1 cell growth increased with increasing concentrations of Gln from 0 to 2 mM. Compared with 0 mM Gln, 2 mM Gln increased (P < 0.05) the amounts of phosphorylated 4E-binding protein 1 (4E-BP1) and ribosomal protein S6 kinase (p70S6 kinase) proteins but did not affect abundances of total or phosphorylated AMPK protein. Gln also increased mRNA levels for Bcl-2, mTOR, p70S6 kinase, 4E-BP1, COX7C, ASCT2, ODC, SGLT-1, CFTR, Na+/K+-ATPase, HSP70, and ZO-1. Similarly, cells cultured with Rap and Gln exhibited higher (P < 0.05) abundances of phosphorylated 4E-BP1 and p70S6 kinase proteins than the Rap-only group, whereas abundances of phosphorylated mTOR and 4E-BP1 proteins were increased when AMPK was inhibited by compound C. Conversely, the amount of phosphorylated AMPK increased when mTOR was inhibited by Rap, suggesting a negative cross-talk between mTOR and AMPK. Collectively, these results indicate that Gln stimulates enterocyte growth by activating the mTOR signaling pathway independently of AMPK. © 2014 Springer-Verlag Wien.
语种：
英文
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脂多糖刺激对仔猪下丘脑-垂体-肾上腺轴TLR4信号通路关键基因表达的影响
作者：
陈少魁;刘玉兰;李权;朱惠玲;侯永清;...
作者机构：
[陈少魁; 刘玉兰; 李权; 朱惠玲; 侯永清; 吴欢听; 王秀英; 刘称] 武汉轻工大学动物营养与饲料科学湖北省重点实验室,武汉430023
会议名称：
中国畜牧兽医学会动物营养学分会第七届中国饲料营养学术研讨会
会议时间：
2014-10-16
会议地点：
郑州
会议主办单位：
中国畜牧兽医学会动物营养学分会
会议论文集名称：
中国畜牧兽医学会动物营养学分会第七届中国饲料营养学术研讨会论文集
关键词：
仔猪;脂多糖;HPA轴;TLR4信号通路
摘要：
本试验旨在研究脂多糖(lipopolysaccharide,LPS)刺激对仔猪下丘脑-垂体-肾上腺(HPA)轴Toll样受体4(toll-like receptor 4,TLR4)信号通路关键基因表达的影响。选取12头(13.76±1.13)kg杜×长×大断奶仔猪,分成2个处理组,每个处理组6个重复。预试10 d后,试验组注射100μg/kg体重的LPS,对照组注射等量的生理盐水。注射LPS或生理
语种：
中文
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天冬氨酸对脂多糖刺激仔猪结肠黏膜结构和屏障功能的影响
作者：
刘称;朱惠玲;刘玉兰;皮定安;冷炜博;...
期刊：
中国畜牧杂志 ,2015年51(01):38-42 ISSN：0258-7033
作者机构：
武汉轻工大学动物营养与饲料科学湖北省重点实验室,湖北武汉,430023;[侯永清; 吴欢听; 王秀英; 冷炜博; 刘称; 皮定安; 刘玉兰; 朱惠玲] 武汉轻工大学
关键词：
天冬氨酸;脂多糖;结肠黏膜;屏障功能;仔猪
摘要：
本试验旨在研究天冬氨酸(ASP)对脂多糖(LPS)刺激仔猪结肠黏膜结构和屏障功能的影响。试验选用24头仔猪,4个处理组分别为对照组、LPS组、0.5%ASP组、1.0%ASP组。试验第19天,分别给LPS组、0.5%ASP组、1.0%ASP组试验猪注射100μg/kg体重的LPS,对照组注射等量的生理盐水。注射后24 h屠宰,取结肠样品待测。结果表明:对照组仔猪结肠形态结构完整;注射LPS后,结肠上皮部分脱落;ASP组轻度脱落,与LPS组相比,0.5%ASP组结肠隐窝深度显著升高(P<0.05);0.5%和1.0%ASP可显著缓解LPS刺激导致的结肠杯状细胞数目的增多(P<0.05);0.5%ASP具有提高结肠中性粒细胞密度的趋势(P<0.10)。0.5%和1.0%ASP可显著缓解LPS刺激导致的结肠和血浆二胺氧化酶活性的降低(P<0.05)。以上研究结果表明,日粮添加ASP可有效阻止LPS对结肠结构和屏障功能的损伤。
语种：
中文
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Beneficial roles of dietary oleum cinnamomi in alleviating intestinal injury
作者：
Wang, Lei;Hou, Yongqing* ;Yi, Dan;Ding, Binying;Zhao, Di;...
期刊：
Frontiers in Bioscience-Landmark ,2015年20(5):814-828 ISSN：2768-6701
通讯作者：
Hou, Yongqing
作者机构：
[Gong, Joshua; Liu, Yulan; Hou, Yongqing; Wang, Lei; Wu, Guoyao; Ding, Binying; Zhao, Di; Yi, Dan; Wang, Zhongxing; Zhu, Huiling] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Peoples R China.;[Gong, Joshua] Agr & Agri Food Canada, Guelph Food Res Ctr, Guelph, ON N1G 5C9, Canada.;[Wu, Guoyao; Assaad, Houssein] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.;[Hou, Yongqing] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
通讯机构：
[Hou, Yongqing] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
关键词：
Intestinal mucosa;Lipopolysaccharide;Oleum cinnamomi;Piglets;Review
摘要：
Cinnamon is a traditional herb used for treatment of many human diseases. The most important chemical compounds of the essential oil are cinnamaldehyde and eugenol. Oleum cinnamomi (OCM, cinnamon oil) is increasingly used as a feed additive to animal diets. Beneficial effects of OCM in protecting tissues from inflammation and injury by endogenous and exogenous agents (such as hydrogen peroxide and lipopolysaccharide (LPS)) may result, in part, from its action on regulating amino acid metabolism in cells to favor the synthesis of glutathione (a major low-molecular-weight antioxidant) from cysteine, glycine and glutamate. In support of this notion, results of recent studies indicate that supplementing OCM (50 mg/kg diet) to a corn- and soybean meal-based diet for piglets weaned at 21 days of age enhances intestinal anti-oxidative capacity and reduces the incidence of diarrhea. Additionally, dietary supplementation with OCM ameliorates LPS-induced mucosal barrier dysfunction and mucosal damage in the small intestine. OCM holds great promise for protecting the gut from injury under conditions of inflammation, infections, and oxidative stress. © 2015, Frontiers in Bioscience. All rights reserved.
语种：
英文
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Asparagine attenuates intestinal injury, improves energy status and inhibits AMP-activated protein kinase signalling pathways in weaned piglets challenged with Escherichia coli lipopolysaccharide
作者：
Wang, Xiuying;Liu, Yulan* ;Li, Shuang;Pi, Dingan;Zhu, Huiling;...
期刊：
British Journal of Nutrition ,2015年114(4):553-565 ISSN：0007-1145
通讯作者：
Liu, Yulan
作者机构：
[Liu, Yulan; Leng, Weibo; Li, Shuang; Hou, Yongqing; Shi, Haifeng; Pi, Dingan; Wang, Xiuying; Zhu, Huiling] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
通讯机构：
[Liu, Yulan] W;Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
关键词：
Asparagine;Intestine;Energy status;Weaned piglets;Lipopolysaccharide
摘要：
The intestine requires a high amount of energy to maintain its health and function; thus, energy deficits in intestinal mucosa may lead to intestinal damage. Asparagine (Asn) is a precursor for many other amino acids such as aspartate, glutamine and glutamate, which can be used to supply energy to enterocytes. In the present study, we hypothesise that dietary supplementation of Asn could alleviate bacterial lipopolysaccharide (LPS)-induced intestinal injury via improvement of intestinal energy status. A total of twenty-four weaned piglets were assigned to one of four treatments: (1) non-challenged control; (2) LPS+0 % Asn; (3) LPS+0.5 % Asn; (4) LPS+1.0 % Asn. On day 19, piglets were injected with LPS or saline. At 24 h post-injection, piglets were slaughtered and intestinal samples were collected. Asn supplementation improved intestinal morphology, indicated by higher villus height and villus height:crypt depth ratio, and lower crypt depth. Asn supplementation also increased the ratios of RNA:DNA and protein:DNA as well as disaccharidase activities in intestinal mucosa. In addition, Asn supplementation attenuated bacterial LPS-induced intestinal energy deficits, indicated by increased ATP and adenylate energy charge levels, and decreased AMP:ATP ratio. Moreover, Asn administration increased the activities of key enzymes involved in the tricarboxylic acid cycle, including citrate synthase, isocitrate dehydrogenase and α-ketoglutarate dehydrogenase complex. Finally, Asn administration decreased the mRNA abundance of intestinal AMP-activated protein kinase-α1 (AMPKα1), AMPKα2, silent information regulator 1 (SIRT1) and PPARγ coactivator-1α (PGC1α), and reduced intestinal AMPKα phosphorylation. Collectively, these results indicate that Asn supplementation alleviates bacterial LPS-induced intestinal injury by modulating the AMPK signalling pathway and improving energy status. Copyright © The Authors 2015.
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英文
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