脂多糖对仔猪肠道、肝脏和肌肉组织NODs信号通路关键基因表达的影响
作者:
涂治骁;刘玉兰;吴欢听;王秀英;朱惠玲;...
作者机构:
武汉轻工大学动物营养与饲料科学湖北省重点实验室,湖北武汉430023
会议名称:
中国畜牧兽医学会动物营养分会第七届中国猪营养学术研讨会
会议时间:
2015-10-01
会议地点:
重庆
会议论文集名称:
中国畜牧兽医学会动物营养分会第七届中国猪营养学术研讨会论文集
关键词:
脂多糖;仔猪
摘要:
本试验旨在研究脂多糖(Lipopolysaccharide,LPS)刺激对仔猪核苷酸结合寡聚化结构域蛋白(NODs)信号通路关键基因表达的影响.试验选取12头杜×长×大断奶仔猪,分成2个处理组,每个处理组6个重复.试验组注射100 μg/kg体重的LPS,对照组注射等量的生理盐水.注射LPS或生理盐水4h后,屠宰仔猪,取空肠、回肠、肝脏、背最长肌和腓肠肌,应用实时荧光定量PCR技术测定NODs信号通路关键基因mRNA表达水平.包括NOD1、NOD2、受体相互作用蛋白激酶2 (RIPK2)、核因子-κB(NF-κB)、肿瘤坏死因子-α(TNF-α)、矢车菊苷β1(ACAP1)和Erbb2相互作用蛋白(ERBB2IP)mRNA表达水平.结果表明:与对照组相比,LPS刺激显著提高了肝脏NOD1、NOD2、RIPK2、NF-κB和TNF-α,背最长肌ⅣOD2、RIPK2和TNF-α,腓肠肌NOD2、RIPK2、NF-κB和TNF-α,空肠RIPK2和TNF-α的mRNA表达量(P<0.05),LPS刺激有提高回肠RIPK2和NF-κB mRNA表达量的趋势(P<0.10);同时,LPS刺激显著降低组织中的负调控因子,如空肠、回肠和肝脏ACAP1,空肠ERBB2IP的mRNA的表达量(P<0.05).因此,综上表明,LPS激活仔猪肠道、肝脏和肌肉组织中NODs信号通路关键基因的表达.
语种:
中文
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天冬酰胺通过TLR4和NOD信号通路对LPS诱导的仔猪肝脏损伤的调控作用
作者:
吴欢听;刘玉兰;石海峰;李爽;皮定安;...
作者机构:
[吴欢听; 刘玉兰; 石海峰; 李爽; 皮定安; 冷炜博; 朱慧玲; 侯永清] 武汉轻工大学动物营养与饲料科学湖北省重点实验室,武汉430023
会议名称:
中国畜牧兽医学会动物营养学分会第七届中国饲料营养学术研讨会
会议时间:
2014-10-16
会议地点:
郑州
会议主办单位:
中国畜牧兽医学会动物营养学分会
会议论文集名称:
中国畜牧兽医学会动物营养学分会第七届中国饲料营养学术研讨会论文集
关键词:
天冬酰胺;仔猪;肝脏损伤;炎性介质
摘要:
本试验研究了天冬酰胺(Asn)对脂多糖(LPS)刺激仔猪肝脏损伤的保护作用,并从toll样受体4(TLR4)和核苷酸结合寡聚域蛋白(NOD)信号通路的角度探讨其机理。选取48头断奶仔猪,分为4个处理:(1)对照组(基础日粮);(2)LPS组(基础日粮+LPS);(3)0.5%Asn组(基础日粮+0.5%Asn+LPS);(4)1.0%Asn组(基础日粮+1.0%Asn+LPS)。实验第20天,2、
语种:
中文
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Dietary supplementation with tributyrin alleviates intestinal injury in piglets challenged with intrarectal administration of acetic acid
作者:
Hou, Yongqing* ;Wang, Lei;Yi, Dan;Ding, Binying;Chen, Xing;...
期刊:
British Journal of Nutrition ,2014年111(10):1748-1758 ISSN:0007-1145
通讯作者:
Hou, Yongqing
作者机构:
[Liu, Yulan; Hou, Yongqing; Chen, Xing; Wang, Lei; Wang, Qingjing; Ding, Binying; Yin, Yulong; Yi, Dan; Zhu, Huiling] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Gong, Joshua] Agr & Agri Food Canada, Guelph Food Res Ctr, Guelph, ON N1G 5C9, Canada.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.
通讯机构:
[Hou, Yongqing] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
关键词:
Acetic acid;Colon;Intestinal injury;Piglets;Tributyrin
摘要:
Tributyrin (TBU) is a good dietary source of butyrate and has beneficial effects on the maintenance of normal intestinal morphology. The present study tested the hypothesis that dietary TBU supplementation could alleviate intestinal injury in the acetic acid (ACA)-induced porcine model of colitis. A total of eighteen piglets (25Â d old) were randomly allocated to one of three treatment groups (control, ACA and TBU). The control and ACA groups were fed a basal diet and the TBU group was fed the basal diet supplemented with 0·1Â % TBU. On day 15 of the trial, under anaesthesia, a soft catheter was inserted into the rectum of piglets (20-25Â cm from the anus), followed by administration of either saline (control group) or ACA (10Â ml of 10Â % ACA solution for ACA and TBU groups). On day 22 of the trial, after venous blood samples were collected, piglets were killed to obtain mid-ileum and mid-colon mucosae. Compared with the control group, the ACA group exhibited an increase (P<Â 0·05) in lymphocyte counts, creatinine, PGE2, and malondialdehyde concentrations and diamine oxidase and inducible NO synthase activities in the plasma and lymphocyte density in the colon and a decrease in insulin concentrations and glutathione peroxidase activity, ileal villus height:crypt depth ratios and goblet cell numbers in the colon. These adverse effects of ACA were attenuated by TBU supplementation. Moreover, TBU prevented the ACA-induced increase in caspase-3 levels while enhancing claudin-1 protein and epidermal growth factor receptor (EGFR) mRNA expression in the colonic mucosa. Collectively, these results indicate that dietary supplementation with 0·1Â % TBU alleviates ACA-induced intestinal injury possibly by inhibiting apoptosis, promoting tight-junction formation and activating EGFR signalling. © 2014 The Authors.
语种:
英文
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Aspartate alleviates liver injury and regulates mRNA expressions of TLR4 and NOD signaling-related genes in weaned pigs after lipopolysaccharide challenge
作者:
Leng, Weibo;Liu, Yulan* ;Shi, Haifeng;Li, Shuang;Zhu, Huiling;...
期刊:
Journal of Nutritional Biochemistry ,2014年25(6):592-599 ISSN:0955-2863
通讯作者:
Liu, Yulan
作者机构:
[Liu, Yulan; Leng, Weibo; Shi, Haifeng; Li, Shuang; Hou, Yongqing; Pi, Dingan; Zhu, Huiling] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Gong, Joshua] Agr & Agri Food Canada, Guelph Food Res Ctr, Guelph, ON N1G 5C9, Canada.
通讯机构:
[Liu, Yulan] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
关键词:
Aspartate;Weaned pigs;Lipopolysaccharide;Pro-inflammatory cytokines;Toll-like receptor 4;Nucleotide-binding oligomerization domain protein;Liver injury
摘要:
Pro-inflammatory cytokines play a critical role in many models of liver injury. In addition, aspartate (Asp) plays an important role in many biological and physiological processes including liver physiology. We hypothesized that Asp could alleviate lipopolysaccharide (LPS)-induced liver injury. Forty-eight weanling pigs were assigned to four treatments including: (1) non-challenged control; (2) LPS challenged control; (3) LPS+0.5% Asp; (4) LPS+1.0% Asp. After 20-d feeding with control (0% Asp), 0.5% or 1.0% Asp supplemented diets, pigs were injected with saline or LPS. At 4 (early phase) and 24 h (late phase) post-injection, blood and liver samples were obtained. Asp attenuated liver injury indicated by reduced serum aspartate aminotransferase activity and increased ratio of serum alanine aminotransferase and aspartate aminotransferase at 24 h, and less severe histological liver damage induced by LPS challenge at 4 or 24 h. In addition, Asp supplementation to LPS challenged pigs decreased mRNA expressions of tumor necrosis factor (TNF)-alpha and cyclooxygenase-2 linearly and quadratically at 4 h, and increased mRNA expressions of these pro-inflammatory mediators linearly and quadratically at 24 h. Finally, Asp decreased mRNA expression of toll-like receptor 4 (TLR4) signaling related genes (TLR4, myeloid differentiation factor 88, IL-1 receptor-associated kinase 1, TNF-alpha receptor-associated factor (6), nucleotide-binding oligomerization domain protein (NOD) signaling related genes (NOD1, NOD2 and receptor-interacting serine/threonine-protein kinase 2) and nuclear factor-kappa B p65 linearly or quadratically at 4 h. However, Asp increased mRNA expressions of these signaling molecules linearly or quadratically at 24 h. These results indicate that, at early and late phases of LPS challenge, Asp exerts opposite regulatory effects on mRNA expression of hepatic pro-inflammatory cytokines and TLR4 and NOD signalling related genes, and improves liver integrity. (C) 2014 Elsevier Inc. All rights reserved.
语种:
英文
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天冬氨酸对脂多糖刺激断奶仔猪肠道形态结构和黏膜抗氧化能力的影响
作者:
冷炜博;刘玉兰;李爽;石海峰;朱惠玲;...
期刊:
中国畜牧杂志 ,2014年50(11):32-36 ISSN:0258-7033
作者机构:
武汉轻工大学,动物营养与饲料科学湖北省重点实验室,湖北武汉430023;[侯永清; 李爽; 冷炜博; 皮定安; 朱惠玲; 刘玉兰; 石海峰] 武汉轻工大学
关键词:
天冬氨酸;脂多糖;肠黏膜;抗氧化;断奶仔猪
摘要:
本试验旨在研究天冬氨酸(ASP)对脂多糖(LPS)刺激断奶仔猪肠道结构和黏膜抗氧化能力的影响。选取(21±1)d日龄断奶、平均体重(7.37±0.04)kg的杜×长×大三元杂交仔猪24头,随机分为对照组(基础日粮)、LPS组(基础日粮+LPS)、0.5%ASP组(基础日粮+0.5%ASP+LPS)、1.0%ASP组(基础日粮+1.0%ASP+LPS)4组,试验期24 d。试验第24天,分别给3个试验组的猪注射100μg/kg体重的LPS,对照组注射等量的生理盐水。注射后24 h屠宰,取小肠样品待测。结果表明:0.5%或1.0%ASP可显著缓解LPS刺激导致的空肠绒毛高度降低、隐窝深度升高和绒毛高度/隐窝深度的降低,增加回肠的绒毛高度、绒毛高度/隐窝深度(P<0.05);0.5%ASP对空肠超氧化物歧化酶、回肠谷胱甘肽过氧化物酶活性有提高的趋势(P<0.10);0.5%或1.0%ASP显著降低了空肠和回肠丙二醛含量(P<0.05)。以上研究结果显示,ASP有提高仔猪肠黏膜抗氧化相关酶的酶活趋势,降低过氧化物的产生,从而改善肠道形态结构。
语种:
中文
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Dietary supplementation of aspartate enhances intestinal integrity and energy status in weanling piglets after lipopolysaccharide challenge
作者:
Pi, Dingan;Liu, Yulan* ;Shi, Haifeng;Li, Shuang;Odle, Jack;...
期刊:
Journal of Nutritional Biochemistry ,2014年25(4):456-462 ISSN:0955-2863
通讯作者:
Liu, Yulan
作者机构:
[Liu, Yulan; Leng, Weibo; Shi, Haifeng; Li, Shuang; Hou, Yongqing; Chen, Feng; Pi, Dingan; Zhu, Huiling] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Odle, Jack; Lin, Xi] N Carolina State Univ, Dept Anim Sci, Lab Dev Nutr, Raleigh, NC 27695 USA.
通讯机构:
[Liu, Yulan] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
关键词:
Aspartate;Intestine;Energy status;Weanling piglets;Lipopolysaccharide
摘要:
The intestine has a high requirement for ATP to support its integrity, function and health, and thus, energy deficits in the intestinal mucosa may play a critical role in intestinal injury. Aspartate (Asp) is one of the major sources of ATP in mammalian enterocytes via mitochondrial oxidation. We hypothesized that dietary supplementation of Asp could attenuate lipopolysaccharide (LPS)-induced intestinal damage via modulation of intestinal energy status. Twenty-four weanling piglets were allotted to one of four treatments: (1) nonchallenged control, (2) LPS-challenged control, (3) LPS+0.5% Asp treatment, and (4) LPS+1.0% Asp treatment. On day 19, pigs were injected with saline or LPS. At 24 h postinjection, pigs were killed and intestinal samples were obtained. Asp attenuated LPS-induced intestinal damage indicated by greater villus height and villus height/crypt depth ratio as well as higher RNA/DNA and protein/DNA ratios. Asp improved intestinal function indicated by increased intestinal mucosal disaccharidase activities. Asp also improved intestinal energy status indicated by increased ATP, ADP and total adenine nucleotide contents, adenylate energy charge and decreased AMP/ATP ratio. In addition, Asp increased the activities of tricarboxylic acid cycle key enzymes including citrate synthase, isocitrate dehydrogenase and alpha-oxoglutarate dehydrogenase complex. Moreover, Asp down-regulated mRNA expression of intestinal AMP-activated protein kinase alpha 1 (AMPK alpha 1), AMPK alpha 2, silent information regulator 1 (SIRT1) and peroxisome proliferator activated receptor gamma coactivator-1 alpha (PGC1 alpha) and decreased intestinal AMPK alpha phosphorylation. These results indicate that Asp may alleviate LPS-induced intestinal damage and improve intestinal energy status. (C) 2014 Elsevier Inc. All rights reserved.
语种:
英文
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脂多糖刺激对仔猪下丘脑-垂体-肾上腺轴Toll样受体4信号通路关键基因表达的影响
作者:
陈少魁;刘玉兰;李权;朱惠玲;侯永清;...
期刊:
动物营养学报 ,2014年26(11):3356-3361 ISSN:1006-267X
作者机构:
[陈少魁; 刘玉兰; 李权; 朱惠玲; 侯永清; 吴欢听; 王秀英; 刘称] 武汉轻工大学
关键词:
仔猪;脂多糖;HPA轴;TLR4信号通路
摘要:
本试验旨在研究脂多糖(LPS)刺激对仔猪下丘脑-垂体-肾上腺(HPA)轴Toll样受体4(TLR4)信号通路关键基因表达的影响。选取12头断奶仔猪,分成2个组,每个组6个重复。试验组注射100μg/kg体重的LPS,对照组注射等量的生理盐水。注射LPS或生理盐水4 h后采血,测定血浆中与应激相关激素的含量;采血后屠宰仔猪,取下丘脑、垂体、肾上腺,测定TLR4信号通路关键基因的mRNA表达水平,包括TLR4、髓样分化因子88(My D88)、白细胞介素-1受体相关激酶1(IRAK1)、肿瘤坏死因子受体相关因子6(TRAF6)和核转录因子-κB(NF-κB)mRNA表达水平。结果表明:与对照组相比,1)LPS刺激导致血浆肿瘤坏死因子-α、皮质醇和促肾上腺皮质激素含量显著上升(P<0.05)。2)LPS刺激导致TLR4信号通路关键基因TLR4在下丘脑、垂体、肾上腺中mRNA表达水平显著升高(P<0.05);My D88在垂体、肾上腺中mRNA表达水平显著升高(P<0.05),在下丘脑中mRNA表达水平有升高的趋势(P<0.10);IRAK1在垂体、肾上腺中mRNA表达水平有升高的趋势(P<0.10);TRAF6在下丘脑、垂体、肾上腺中mRNA表达水平显著升高(P<0.05);NF-κB在垂体中mRNA表达水平显著升高(P<0.05)。试验结果表明LPS刺激激活了HPA轴,诱导了HPA轴的TLR4信号通路关键基因的表达。
语种:
中文
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天冬酰胺对脂多糖刺激仔猪肠黏膜结构及抗氧化能力的影响
作者:
皮定安;刘玉兰;李爽;石海峰;朱惠玲;...
期刊:
中国畜牧杂志 ,2014年50(05):60-64 ISSN:0258-7033
作者机构:
武汉轻工大学动物营养与饲料科学湖北省重点实验室,湖北武汉,430023;[丁斌鹰; 侯永清; 李爽; 冷炜博; 陈逢; 朱惠玲; 刘玉兰; 皮定安; 石海峰] 武汉轻工大学
关键词:
天冬酰胺;脂多糖;肠黏膜结构;抗氧化;仔猪
摘要:
试验旨在研究天冬酰胺(Asn)对脂多糖(LPS)刺激仔猪肠道结构及抗氧化能力的影响。选用24头断奶仔猪,分为对照组、LPS组、0.5%Asn组(0.5%Asn+LPS)和1.0%Asn组(1.0%Asn+LPS)。第24天,各组分别注射LPS或等量生理盐水24 h后屠宰。结果表明:LPS刺激导致空肠绒毛高度显著降低,日粮中添加0.5%或1.0%Asn显著降低了空肠、回肠的隐窝深度,提高了空肠、回肠的绒毛高度和绒毛高度/隐窝深度的比值(P<0.05);LPS刺激也导致空肠超氧化物歧化酶(SOD)活性和氧化型谷胱甘肽(GSSG)/还原性谷胱甘肽(GSH)比值显著升高(P<0.05);日粮中添加0.5%或1.0%Asn显著提高了空肠、回肠GSH的含量(P<0.05),降低了空肠、回肠GSSG/GSH的比值(P<0.01)和丙二醛(MDA)的含量(P<0.05)。结果显示,Asn缓解了LPS刺激引起的肠道结构损伤和抗氧化能力下降。
语种:
中文
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Effects of l-proline on the growth performance, and blood parameters in weaned lipopolysaccharide (LPS)-challenged pigs
作者:
Kang, Ping;Zhang, Lili;Hou, Yongqing* ;Ding, Binying;Yi, Dan;...
期刊:
Animal Bioscience ,2014年27(8):1150-1156 ISSN:2765-0189
通讯作者:
Hou, Yongqing
作者机构:
[Zhang, Lili; Liu, Yulan; Kang, Ping; Hou, Yongqing; Wang, Lei; Ding, Binying; Yin, Yulong; Yi, Dan; Zhu, Huiling] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Yin, Yulong] Chinese Acad Sci, Inst Subtrop Agr, Changsha 410125, Hunan, Peoples R China.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.
通讯机构:
[Hou, Yongqing] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
关键词:
Kang, Ping;Zhang, Lili;Hou, Yongqing;Ding, Binying;Yi, Dan;Wang, Lei;Zhu, Huiling;Liu, Yulan;Yin, Yulong;Wu, Guoyao;L-proline;Growth Performance;Blood Parameters;Gastrointestinal Tract Digestibility;Early Weaned Pigs;Lipopolysaccharide
摘要:
This trail was conducted to study the effect of L-proline on the growth performance, and blood parameter in the weaned lipopolysaccharide (LPS)-challenged pigs. Thirty six pigs (9.13±0.85 kg) were assigned randomly to dietary treatments in a 2×3 factorial arrangement in a 20-d growth assay. Factors were intraperitoneal injection with saline or LPS, and three dietary L-proline supplement levels (0%, 0.5%, or 1.0%). On d 10, blood samples were collected at 3 h after LPS (100 μg LPS/kg body weight [BW]) or saline injection. On d 20 of the trial, all pigs were orally administrated D-xylose (0.1 g/kg BW) at 2 h, and blood samples were collected at 3 h after LPS or saline injection. As a result, dietary supplementation with 0.5% proline had a tendency to increase average daily gain (ADG) in piglets during d 10 to 20 (p = 0.088). Without LPS challenge, dietary supplementation with 1.0% proline had no effect on growth hormone (GH) concentrations on d 10 (p>0.05), but decreased it after LPS challenge (p<0.05). There was LPS challenge× proline interaction for GH concentrations on d 10 (p<0.05). Dietary supplementation with 1.0% proline decreased glucagon concentration on d 10 after LPS challenge (p<0.05). In addition, dietary supplementation with proline increased superoxide dismutase (SOD) activity significantly on d 10 and 20 (p<0.05), and 1.0% proline increased heat shock proteins-70 concentration on d 10 (p<0.05). Moreover, proline supplementation increased diamine oxidase (DAO) concentrations after LPS challenge (p<0.05). There was LPS challenge×proline interaction for DAO (p<0.05). Furthermore, dietary supplementation with 1.0% proline increased the D-xylose level when no LPS challenge (p<0.05). These results indicate that proline supplementation could improve growth performance, increase SOD activities, and has a positive effect on the gastrointestinal tract digestibility in early weaned pigs. © 2014 by Asian-Australasian Journal of Animal Sciences.
语种:
英文
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Dietary N-acetylcysteine supplementation alleviates liver injury in lipopolysaccharide-challenged piglets
作者:
Yi, Dan;Hou, Yongqing* ;Wang, Lei;Ding, Binying;Yang, Zhengguo;...
期刊:
British Journal of Nutrition ,2014年111(1):46-54 ISSN:0007-1145
通讯作者:
Hou, Yongqing
作者机构:
[Long, Minhui; Liu, Yulan; Yang, Zhengguo; Hou, Yongqing; Wang, Lei; Ding, Binying; Li, Jiao; Yi, Dan] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.;[Wu, Guoyao] China Agr Univ, State Key Lab Anim Nutr, Beijing 100193, Peoples R China.
通讯机构:
[Hou, Yongqing] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
关键词:
N-Acetylcysteine;Piglets;Lipopolysaccharide;Liver injury
摘要:
The present study was carried out to determine whether N-acetylcysteine (NAC) could modulate liver injury in a lipopolysaccharide (LPS)-challenged piglet model. For this purpose, eighteen piglets were randomly assigned to the control, LPS or NAC group. Piglets in the control and LPS groups were fed a basal diet, whereas those in the NAC group were fed the basal diet supplemented with 500mg/kg NAC. On days 10, 13 and 20 of the trial, the LPS-and NAC-treated piglets were intraperitoneally administered LPS (100μg/kg body weight), while the control group was administered the same volume of saline. On day 20 of the trial, blood samples were obtained 3h after LPS or saline injection. On day 21, the piglets were killed to collect liver samples. Dietary NAC supplementation attenuated LPS-induced liver histomorphological abnormalities. Compared with the control group, in the LPS-challenged piglets, the activities of alanine aminotransferase and aspartate aminotransferase and the concentrations of H2O2, TNF-α, IL-6 and PGE2 were dramatically increased in the plasma and the activity of superoxide dismutase in the plasma and that of glutathione peroxidase in the liver were significantly decreased. The LPS challenge also increased the concentration of AMP and the ratio of AMP:ATP, but decreased adenylate energy charges and the levels of ATP and ADP. These adverse effects of the LPS challenge were ameliorated by NAC supplementation. Moreover, NAC inhibited the LPS-induced increases in the abundance of liver heat shock protein 70 and NF-κB proteins. In conclusion, these results suggest that dietary NAC supplementation alleviates LPS-induced liver injury by reducing the secretion of pro-inflammatory cytokines, increasing the antioxidative capacity and improving energy metabolism. © 2013 The Authors.
语种:
英文
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TLR4信号通路关键基因在断奶仔猪不同组织中的mRNA表达
作者:
陈逢;刘玉兰;李权;朱惠玲;侯永清;...
期刊:
中国畜牧杂志 ,2013年49(17):53-57 ISSN:0258-7033
作者机构:
[陈逢; 刘玉兰; 李权; 朱惠玲; 侯永清; 易丹; 陈洪波] 武汉工业学院动物营养与饲料科学湖北省重点实验室
会议名称:
中国畜牧兽医学会动物营养学分会第十一次全国动物营养学术研讨会
会议时间:
2012-10-19
会议地点:
长沙
会议论文集名称:
中国畜牧兽医学会动物营养学分会第十一次全国动物营养学术研讨会论文集
关键词:
TLR4信号通路;实时荧光定量PCR;断奶仔猪
摘要:
本试验旨在研究Toll样受体4(TLR4)信号通路关键基因在断奶仔猪不同组织的分布情况。选择12头杜×长×大断奶仔猪,屠宰,取脾脏、胸腺、肠道淋巴结、下丘脑、垂体、肾上腺、肝脏、腓肠肌、皮下脂肪、空肠和回肠组织。应用实时荧光定量PCR技术测定TLR4信号通路关键基因,包括TLR4、髓样分化因子(MyD88)、IL-1受体相关激酶1(IRAK1)、肿瘤坏死因子受体相关因子6(TRAF6)、核因子-κB(NF-κB)和肿瘤坏死因子(TNF)-α在各组织的mRNA表达水平。结果表明:TLR4信号通路关键基因在所检测的11个组织中均有表达,并且组织分布规律基本一致。总体看来,主要在免疫组织(脾脏、胸腺、淋巴结)表达量较高,在皮下脂肪和肠道(空肠、回肠)表达量居中,在其他组织中表达量较低。TLR4信号通路关键基因在不同组织中表达差异较大,可能与仔猪各组织对病原的识别和抵抗能力有关。
语种:
中文
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Fish Oil Alleviates Activation of the Hypothalamic-Pituitary-Adrenal Axis Associated with Inhibition of TLR4 and NOD Signaling Pathways in Weaned Piglets after a Lipopolysaccharide Challenge
作者:
Liu, Yulan* ;Chen, Feng;Li, Quan;Odle, Jack;Lin, Xi;...
期刊:
The Journal of Nutrition ,2013年143(11):1799-1807 ISSN:0022-3166
通讯作者:
Liu, Yulan
作者机构:
[Liu, Yulan; Li, Quan; Hou, Yongqing; Shi, Haifeng; Chen, Feng; Pi, Dingan; Hong, Yu; Zhu, Huiling] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan, Peoples R China.;[Odle, Jack; Lin, Xi] N Carolina State Univ, Dept Anim Sci, Lab Dev Nutr, Raleigh, NC 27695 USA.
通讯机构:
[Liu, Yulan] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan, Peoples R China.
摘要:
Long-chain n-3 (ω-3) polyunsaturated fatty acids exert beneficial effects in neuroendocrine dysfunctions in animal models and clinical trials. However, the mechanism(s) underlying the beneficial effects remains to be elucidated. We hypothesized that dietary treatment with fish oil (FO) could mitigate LPS-induced activation of the hypothalamic-pituitary-adrenal (HPA) axis through inhibition of Toll-like receptor 4 and nucleotide-binding oligomerization domain protein signaling pathways. Twentyfour weaned pigs were used in a 2 × 2 factorial design, and the main factors consisted of diet (5% corn oil vs. 5% FO) and immunological challenge (saline vs. LPS). After 21 d of dietary treatment with 5% corn oil or FO diets, pigs were treated with saline or LPS. Blood samples were collected at 0 (preinjection), 2, and 4 h postinjection, and then pigs were humanely killed by intravenous injection of 40 mg/kg body weight sodium pentobarbital for tissue sample collection. FO led to enrichment of eicosapentaenoic acid and docosahexaenoic acid and total n-3 polyunsaturated fatty acids in hypothalamus, pituitary gland, adrenal gland, spleen, and thymus. FO decreased plasma adrenocorticotrophin and cortisol concentrations as well as mRNA expressions of hypothalamic corticotropin releasing hormone and pituitary proopiomelanocortin. FO also reduced mRNA expression of tumor necrosis factor-α in hypothalamus, adrenal gland, spleen, and thymus, and of cyclooxygenase 2 in hypothalamus. Moreover, FO downregulated the mRNA expressions of Toll-like receptor 4 (TLR4) and its downstream molecules, including cluster differentiation factor 14,myeloid differentiation factor 2, myeloid differentiation factor 88, interleukin- 1 receptor-associated kinase 1, tumor necrosis factor-α receptor-associated factor 6, and nuclear factor kappa-light-chainenhancer of activated B cells p65, and also decreased the mRNA expressions of nucleotide-binding oligomerization domain 1, nucleotide-binding oligomerization domain 2, and their adaptor molecule receptor-interacting serine/threonine-protein kinase 2. These results suggested that FOattenuates the activation of the HPA axis induced by LPS challenge. The beneficial effects of FO on the HPA axis may be associated with decreasing the production of brain or peripheral proinflammatory cytokines through inhibition of TLR4 and nucleotide-binding oligomerization domain protein signaling pathways. © 2013 American Society for Nutrition.
语种:
英文
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鱼油对脂多糖刺激仔猪肠黏膜能量代谢的影响
作者:
王秀英;刘玉兰;李藏兰;陈逢;朱惠玲;...
期刊:
中国畜牧杂志 ,2013年49(23):22-25 ISSN:0258-7033
作者机构:
1. 武汉轻工大学动物营养与饲料科学湖北省重点实验室;2. 中国农业大学动物科技学院;3. 福建高龙实业有限公司;[李藏兰] 中国农业大学;[丁斌鹰; 侯永清; 李爽; 王秀英; 朱惠玲; 陈逢; 刘玉兰; 石海峰] 武汉轻工大学
关键词:
鱼油;脂多糖;肠黏膜;能量代谢;仔猪
摘要:
试验研究鱼油对脂多糖(LPS)刺激仔猪肠黏膜能量代谢的影响。选用32头(28±3)d(8.91±0.74)kg的杜洛克×长白×大白仔猪,采用2×2因子设计,主因子包括日粮处理(5%鱼油或5%玉米油)和免疫应激(注射LPS或生理盐水),试验期19 d。试验第19天,每日粮组一半的猪注射100μg/kg BW的LPS,另一半注射生理盐水作对照。注射LPS 4 h后屠宰仔猪,取小肠样品待测。结果表明:鱼油对肠黏膜能量代谢的影响与LPS刺激不存在显著的互作关系;LPS刺激导致结肠三磷酸腺苷(ATP)水平显著下降(P<0.05);鱼油显著提高了结肠三磷酸腺苷(ATP)、一磷酸腺苷(AMP)、腺苷酸池(TAN)水平(P<0.05),对结肠AMP/ATP比值有提高的趋势(P<0.10),鱼油也显著降低了结肠能荷(EC)水平(P<0.05);鱼油和LPS刺激对空肠和回肠能量代谢指标均无显著影响。以上研究结果显示,鱼油可以缓解LPS应激引起的仔猪结肠黏膜能量代谢障碍,从而保护肠黏膜屏障。
语种:
中文
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Fish Oil Increases Muscle Protein Mass and Modulates Akt/FOXO, TLR4, and NOD Signaling in Weanling Piglets After Lipopolysaccharide Challenge
作者:
Liu, Yulan* ;Chen, Feng;Odle, Jack;Lin, Xi;Zhu, Huiling;...
期刊:
The Journal of Nutrition ,2013年143(8):1331-1339 ISSN:0022-3166
通讯作者:
Liu, Yulan
作者机构:
[Liu, Yulan; Shi, Haifeng; Hou, Yongqing; Chen, Feng; Zhu, Huiling] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan, Peoples R China.;[Odle, Jack; Lin, Xi] N Carolina State Univ, Dept Anim Sci, Lab Dev Nutr, Raleigh, NC 27695 USA.;[Yin, Jingdong] China Agr Univ, Coll Anim Sci & Technol, State Key Lab Anim Nutr, Beijing 100094, Peoples R China.
通讯机构:
[Liu, Yulan] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan, Peoples R China.
摘要:
Proinflammatory cytokines play a key role in the pathophysiology of muscle atrophy. In addition, n3 polyunsaturated fatty acids (PUFAs) exert an inhibitory effect on proinflammatory cytokines affecting many inflammatory diseases. We hypothesized that dietary supplementation of fish oil could attenuate lipopolysaccharide (LPS)-induced muscle atrophy.Weanling pigswere used in a 2 × 2 factorial design and themain factors included diet (5% corn oil or 5%fish oil) and immunological challenge (LPS or saline). After 21 d of treatment with either fish oil or corn oil, pigs received an i.p. injection of either saline or LPS. At 4 h postinjection, blood and muscle samples were obtained. Fish oil led to enrichment of eicosapentaenoic acid, docosahexaenoic acid, and total n3 PUFAs in muscles. Fish oil increased muscle protein mass, indicated by a higher protein:DNA ratio in gastrocnemius and longissimus dorsi (LD) muscles. In addition, fish oil increased Akt1 mRNA abundance and decreased Forkhead Box O (FOXO) 1 and FOXO4 mRNA abundance. Fish oil also increased phosphorylation of Akt and FOXO1 in gastrocnemius and LD muscles. Fish oil decreased the mRNA abundance of muscle atrophy F-box (MAFbx) and muscle RING finger 1 in gastrocnemius and LD muscles. Moreover, fish oil reduced the plasma tumor necrosis factor (TNF) a, muscle TNFa, and prostaglandin E2 concentrations, and muscle TNFa and cyclooxygenase 2 (COX2) mRNA abundance. Finally, fish oil downregulated the mRNA abundance ofmuscle toll-like receptor (TLR4) and its downstream signalingmolecules [myeloid differentiation factor 88 (MyD88), TNFa receptor-associated factor 6 (TRAF6), and NF-kB p65], and nucleotide-binding oligomerization domain protein (NOD1), NOD2, and their adaptor molecule [receptor-interacting serine/threonine-protein kinase 2 (RIPK2)]. These results indicate fish oil may suppress muscle proinflammatory cytokine production via regulation of TLR and NOD signaling pathways and therefore improve muscle protein mass, possibly through maintenance of Akt/FOXO signaling. © 2013 American Society for Nutrition.
语种:
英文
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Fish oil attenuates liver injury caused by LPS in weaned pigs associated with inhibition of TLR4 and nucleotide-binding oligomerization domain protein signaling pathways:
作者:
Chen, Feng;Liu, Yulan* ;Zhu, Huiling;Hong, Yu;Wu, Zhifeng;...
期刊:
Innate Immunity ,2013年19(5):504-515 ISSN:1753-4259
通讯作者:
Liu, Yulan
作者机构:
[Liu, Yulan; Wu, Zhifeng; Li, Quan; Hou, Yongqing; Chen, Feng; Hong, Yu; Ding, Binying; Yi, Dan; Zhu, Huiling; Chen, Hongbo] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
通讯机构:
[Liu, Yulan] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
关键词:
Fish oil;lipopolysaccharide;weaned pigs;liver injury;pro-inflammatory cytokines;Toll-like receptor 4;nucleotide-binding oligomerization domain protein
摘要:
This study evaluated whether fish oil exerted a hepatoprotective effect in a LPS-induced liver injury model via regulation of TLR4 and nucleotide-binding oligomerization domain protein (NOD) signaling pathways. Twenty-four piglets were used in a 2 × 2 factorial design, and the main factors included diet (5% corn oil or 5% fish oil) and immunological challenge (LPS or saline). Fish oil resulted in enrichment of eicosapentaenoic acid, docosahexaenoic acid and total (n-3) polyunsaturated fatty acids in liver. Less severe liver injury was observed in pigs fed fish oil, as evidenced by improved serum biochemical parameters and less severe histological liver damage. In addition, higher expression of liver tight junction proteins, and lower hepatocyte proliferation and higher hepatocyte apoptosis were observed in pigs fed fish oil. The improved liver integrity in pigs fed fish oil was concurrent with reduced hepatic mRNA expression of TLR4, myeloid differentiation factor 88, IL-1 receptor-associated kinase 1 and TNF-α receptor-associated factor 6, and NOD1, NOD2 and receptor-interacting serine/threonine-protein kinase 2, as well as reduced hepatic protein expression of NF-κB p65, leading to reduced hepatic pro-inflammatory mediators. These results indicate that fish oil improves liver integrity partially via inhibition of TLR4 and NOD signaling pathways under an inflammatory condition. © 2013 The Author(s).
语种:
英文
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Protective effects of N-acetylcysteine on acetic acid-induced colitis in a porcine model
作者:
Wang, Qingjing;Hou, Yongqing* ;Yi, Dan;Wang, Lei;Ding, Binying;...
期刊:
BMC Gastroenterology ,2013年13(1):1-11 ISSN:1471-230X
通讯作者:
Hou, Yongqing
作者机构:
[Long, Minhui; Liu, Yulan; Hou, Yongqing; Chen, Xing; Wang, Lei; Wang, Qingjing; Ding, Binying; Yi, Dan] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.;[Wu, Guoyao] China Agr Univ, State Key Lab Anim Nutr, Beijing 100193, Peoples R China.
通讯机构:
[Hou, Yongqing] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
关键词:
N-acetylcysteine;Acetic acid;Colon injury;Claudin-1;Epidermal growth factor;Amphiregulin
摘要:
Background: Ulcerative colitis is a chronic inflammatory disease and involves multiple etiological factors. Acetic acid (AA)-induced colitis is a reproducible and simple model, sharing many characteristics with human colitis. N-acetylcysteine (NAC) has been widely used as an antioxidant in vivo and in vitro. NAC can affect several signaling pathways involving in apoptosis, angiogenesis, cell growth and arrest, redox-regulated gene expression, and inflammatory response. Therefore, NAC may not only protect against the direct injurious effects of oxidants, but also beneficially alter inflammatory events in colitis. This study was conducted to investigate whether NAC could alleviate the AA-induced colitis in a porcine model.Methods: Weaned piglets were used to investigate the effects of NAC on AA-induced colitis. Severity of colitis was evaluated by colon histomorphology measurements, histopathology scores, tissue myeloperoxidase activity, as well as concentrations of malondialdehyde and pro-inflammatory mediators in the plasma and colon. The protective role of NAC was assessed by measurements of antioxidant status, growth modulator, cell apoptosis, and tight junction proteins. Abundances of caspase-3 and claudin-1 proteins in colonic mucosae were determined by the Western blot method. Epidermal growth factor receptor, amphiregulin, tumor necrosis factor-alpha (TNF-α), and toll-like receptor 4 (TLR4) mRNA levels in colonic mucosae were quantified using the real-time fluorescent quantitative PCR.Results: Compared with the control group, AA treatment increased (P < 0.05) the histopathology scores, intraepithelial lymphocyte (IEL) numbers and density in the colon, myeloperoxidase activity, the concentrations of malondialdehyde and pro-inflammatory mediators in the plasma and colon, while reducing (P < 0.05) goblet cell numbers and the protein/DNA ratio in the colonic mucosa. These adverse effects of AA were partially ameliorated (P < 0.05) by dietary supplementation with NAC. In addition, NAC prevented the AA-induced increase in caspase-3 protein, while stimulating claudin-1 protein expression in the colonic mucosa. Moreover, NAC enhanced mRNA levels for epidermal growth factor and amphiregulin in the colonic mucosa.Conclusion: Dietary supplementation with NAC can alleviate AA-induced colitis in a porcine model through regulating anti-oxidative responses, cell apoptosis, and EGF gene expression. © 2013 Wang et al.; licensee BioMed Central Ltd.
语种:
英文
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Effect of l-arginine on intestinal mucosal immune barrier function in weaned pigs after Escherichia coli LPS challenge:
作者:
Zhu, H. L.;Liu, Y. L.* ;Xie, X. L.;Huang, J. J.;Hou, Y. Q.
期刊:
Innate Immunity ,2013年19(3):242-252 ISSN:1753-4259
通讯作者:
Liu, Y. L.
作者机构:
[Zhu, H. L.; Liu, Y. L.; Hou, Y. Q.; Xie, X. L.; Huang, J. J.] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
通讯机构:
[Liu, Y. L.] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
关键词:
Lipopolysaccharide;arginine;intestinal mucosal immune barrier function;weaned pigs
摘要:
The effects of l-arginine (Arg) supplementation on intestinal mucosal immune barrier function in weaned pigs after Escherichia coli LPS challenge were evaluated. Twenty-four weaned pigs were allotted to four treatments including: (i) non-challenged control; (ii) LPS-challenged control; (iii) LPS + 0.5% Arg; and (iv) LPS + 1.0% Arg. On d 16, pigs in the LPS, LPS + 0.5% Arg and LPS + 1.0% Arg groups were challenged by injection with 100 μg/kg of body mass LPS, whereas the control group were given sterile saline. At 48 h post-challenge, all pigs were sacrificed for evaluation of small intestinal morphology and mucosal immune barrier function. In the jejunum and ileum, LPS caused villous atrophy and intestinal morphology disruption, whereas 0.5% or 1.0% Arg supplementation mitigated villus atrophy and intestinal morphology impairment caused by LPS challenge. Arg (0.5%) supplementation increased the numbers of IgA-secreting cells, CD8+ and CD4+ T cells in the ileum (P < 0.05). Arg supplementation prevented the elevation of mast cell numbers induced by LPS challenge (P < 0.05). Dietary supplementation of Arg caused a decreased lymphocyte apoptosis of Peyer's patches in pigs challenged by LPS (P < 0.05). These results indicated that Arg supplementation protects and enhances intestinal mucosal immune barrier function and maintains intestinal integrity in weaned pigs after E. coli LPS challenge. © The Author(s) 2012 Reprints and permissions: sagepub.co.uk/journalsPermissions.nav.
语种:
英文
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Effects of Oral Administration of Spermine on the Development of Small Intestine and Growth Performance of Weaned Pigs
作者:
Kang, Ping;Wang, Meng;Hou, Yongqing* ;Yin, Yulong;Ding, Binying;...
期刊:
Journal of Animal and Veterinary Advances ,2012年11(15):2782-2787 ISSN:1680-5593
通讯作者:
Hou, Yongqing
作者机构:
[Liu, Yulan; Kang, Ping; Hou, Yongqing; Wang, Lei; Qiu, Yinsheng; Wang, Meng; Yin, Yulong; Ding, Binying; Yi, Dan; Zhu, Huiling] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Yin, Yulong] Chinese Acad Sci, Inst Subtrop Agr, Key Lab Agroecol Proc Subtrop Reg, Changsha 410125, Hunan, Peoples R China.;[Gong, Joshua] Agr & Agri Food Canada, Guelph Food Res Ctr, Guelph, ON N1G 5C9, Canada.
通讯机构:
[Hou, Yongqing] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
关键词:
D-xylose;Growth performance;Intestinal morphology;Intestinal protein/DNA;Piglets;Spermine
摘要:
The effects of spermine on the development of small intestine and growth performance of newly weaned pigs were investigated. Thirty pigs were used and the treatment of spermine was through oral administration for the 1 st 3 days after weaning with the dose of 0, 300, 600, 900 or 1200 umol per pig daily. The administration of spermine improved Average Daily Feed Intake (ADFI) and Average Daily Gam (ADG) in the periods 0-14 (p<0.05) but hadno significant effects on the ratio of Feed to Gain (F/G) and protein/DNA contents of the duodenal or ileal mucosa. The protein/DNA content of the jejunum was significantly increased at the doses of 300 and 600 umol day"1 spermine but was declined at the higher doses. The villus height in the duodenum (p<0.01) and ileum (p<0.05) increased with an increase in spermine doses. The crypt depth was reduced by spermine in the jejunum and the ratio of villus height/crypt depth was increased by spermine in the duodenum, jejunum and ileum (p<0.05). Additionally, the treatment of spermine at tested doses showed no effect on D-xylose absorption by the small intestine. These results indicate that oral administration of spermine for 3 days after weaning increased ADG and ADFI, enhanced the development of the small intestinal mucosa and improved intestinal morphology. © Medwell Journals, 2012.
语种:
英文
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Protective effects of N-acetylcysteine on intestinal functions of piglets challenged with lipopolysaccharide
作者:
Hou, Yongqing* ;Wang, Lei;Zhang, Wei;Yang, Zhenguo;Ding, Binying;...
期刊:
Amino Acids ,2012年43(3):1233-1242 ISSN:0939-4451
通讯作者:
Hou, Yongqing
作者机构:
[Liu, Yulan; Zhang, Wei; Hou, Yongqing; Yang, Zhenguo; Wang, Lei; Qiu, Yinsheng; Ding, Binying; Zhu, Huiling] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.
通讯机构:
[Hou, Yongqing] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
关键词:
Intestinal functions;Lipopolysaccharide;N-acetylcysteine;Piglets
摘要:
The neonatal small intestine is susceptible to damage by endotoxin, but effective methods for prevention and treatment are lacking. N-acetylcysteine (NAC) is a widely used precursor of L-cysteine for animal cells and plays an important role in protecting cells against oxidative stress. This study was conducted with the lipopolysaccharide (LPS)-challenged piglet model to determine the effects of NAC on intestinal function. Eighteen piglets were randomly allocated into control, LPS and LPS + NAC groups. The control and LPS groups were fed a corn- and soybean meal-based diet, and the LPS + NAC group was fed the basal diet +500 mg/kg NAC. On days 10, 13 and 20 of the trial, the LPS and LPS + NAC groups received intraperitoneal administration of LPS (100 lg/kg BW), whereas the control piglets received saline. On day 20 of the trial, D-xylose (0.1 g<kg BW) was orally administrated to all piglets 2 h after LPS or saline injection, and blood samples were collected 1 h thereafter. One hour blood xylose test was used to measure intestinal absorption capacity and mucosal integrity, and diamine oxidase (DAO) was used as a marker of intestinal injury. On day 21 of the trial, pigs were killed to obtain the intestinal mucosa. Compared to the control, LPS challenge reduced (P<0.05) the concentrations of D-xylose (a marker of intestinal absorption) in plasma, activities of DAO in the jejunal mucosa, the ratio of villus height to crypt depth in the jejunal mucosa, RNA/DNA and protein/DNA in the jejunal and ileal mucosae, while increasing (P<0.05) DAO activity in plasma and caspase-3 expression in the intestinal mucosa. The adverse effects of LPS were partially ameliorated (P<0.05) by NAC supplementation. Moreover, NAC prevented the LPS-induced decrease in claudin-1 and occludin expression in the jejunal and ileal mucosae. Collectively, these results indicate that dietary NAC supplementation alleviates the mucosal damage and improves the absorptive function of the small intestine in LPS-challenged piglets. © 2011 Springer-Verlag.
语种:
英文
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Dietary L-arginine supplementation alleviates liver injury caused by Escherichia coli LPS in weaned pigs:
作者:
Li, Quan;Liu, Yulan* ;Che, Zhengquan;Zhu, Huiling;Meng, Guoquan;...
期刊:
Innate Immunity ,2012年18(6):804-814 ISSN:1753-4259
通讯作者:
Liu, Yulan
作者机构:
[Che, Zhengquan; Liu, Yulan; Li, Quan; Meng, Guoquan; Hou, Yongqing; Chen, Feng; Yin, Yulong; Zhu, Huiling] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Liu, Yulan; Yin, Yulong] Chinese Acad Sci, Inst Subtrop Agr, Changsha, Hunan, Peoples R China.
通讯机构:
[Liu, Yulan] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
关键词:
Arginine;weaned pigs;lipopolysaccharide;Toll-like receptor 4;nuclear factor-kappa B;liver injury
摘要:
This study was conducted to evaluate whether dietary supplementation with L-arginine (Arg) could attenuate Escherichia coli LPS-induced liver injury through the TLR4 signaling pathway in weaned pigs. Eighteen weaned pigs were allotted to three treatments: non-challenged control, LPS challenged control and LPS-+-0.5% Arg. On d 18, pigs were injected with LPS at 100-μg/kg of body weight (BW) or sterile saline. Blood samples were obtained at 4-h post-injection. Pigs were then sacrificed for the collection of liver samples. Arg supplementation (0.5%) alleviated liver morphological impairment, including hepatocyte caryolysis, karyopycnosis and fibroblast proliferation induced by LPS challenge; it mitigated the increase of serum aspartate aminotransferase and alkaline phosphatase activities induced by LPS (P-<-0.05); it prevented the increase of hepatic TNF-α, malondialdehyde contents and mast cell number induced by LPS administration (P-<-0.05); and it attenuated the elevation of hepatic NF-κB and TLR4-positive cell percentages (P-<-0.05). These results indicate that Arg supplementation has beneficial effects in attenuating hepatic morphological and functional injury induced by LPS challenge in piglets. Additionally, it is possible that the protective effects of Arg on the liver are associated with a decreased release of liver pro-inflammatory cytokines and free radicals through inhibiting TLR4 signaling. © The Author(s) 2012 Reprints and permissions: sagepub.co.uk/journalsPermissions.nav.
语种:
英文
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