仔猪溃疡性结肠炎模型的建立
作者：
陈星;王清静;王蕾;朱惠玲;丁斌鹰;...
期刊：
饲料工业 ,2013年34(19):24-27 ISSN：1001-991X
作者机构：
动物营养与饲料科学湖北省重点实验室武汉轻工大学,湖北武汉,430023;[陈星; 丁斌鹰; 侯永清; 王清静; 王蕾; 朱惠玲] 武汉轻工大学
关键词：
仔猪;结肠炎;乙酸
摘要：
试验旨在建立乙酸诱导仔猪溃疡性结肠炎模型。选取18头健康仔猪（杜洛克×长白×大白）,随机分成3组:对照组（3头）、三硝基苯磺酸（TNBS）组（6头）和乙酸组（9头）,分别注射30 mg/kg BW戊巴比妥钠,待完全麻醉后,对照组仔猪直肠灌注10 ml无菌生理盐水,TNBS组分别灌注25 mg/kg BW（3头）和50 mg/kg BW（3头）TNBS的50%乙醇溶液,乙酸组分别灌注10 ml 5%（3头）和10%的乙酸（6头）。随时观察仔猪状态与粪便情况,灌注3 d或7 d后屠宰,并取结肠组织固定,制作病理切片观察损伤变化,分别进行黏膜损伤评分,以判定造模是否成功。结果表明,采用直肠灌注不同浓度TNBS（25 mg/kg BW和50 mg/kg BW）、5%乙酸,均只出现短暂的稀便,病理切片观察只有轻微炎性症状,与对照组没有显著差异;灌注10%乙酸的6头仔猪,根据粪便性状及结肠肉眼观察均显示明显的结肠炎症状,同时通过组织学观察发现,3 d后出现溃疡灶,并有明显的炎性细胞浸润等炎症反应,而7 d后可见溃疡灶已脱落,炎性细胞浸润明显减少,大量成纤维细胞增生。由此可见,直肠灌注10%乙酸能成功诱导仔猪结肠炎。
语种：
中文
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Fish Oil Alleviates Activation of the Hypothalamic-Pituitary-Adrenal Axis Associated with Inhibition of TLR4 and NOD Signaling Pathways in Weaned Piglets after a Lipopolysaccharide Challenge
作者：
Liu, Yulan* ;Chen, Feng;Li, Quan;Odle, Jack;Lin, Xi;...
期刊：
The Journal of Nutrition ,2013年143(11):1799-1807 ISSN：0022-3166
通讯作者：
Liu, Yulan
作者机构：
[Liu, Yulan; Li, Quan; Hou, Yongqing; Shi, Haifeng; Chen, Feng; Pi, Dingan; Hong, Yu; Zhu, Huiling] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan, Peoples R China.;[Odle, Jack; Lin, Xi] N Carolina State Univ, Dept Anim Sci, Lab Dev Nutr, Raleigh, NC 27695 USA.
通讯机构：
[Liu, Yulan] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan, Peoples R China.
摘要：
Long-chain n-3 (ω-3) polyunsaturated fatty acids exert beneficial effects in neuroendocrine dysfunctions in animal models and clinical trials. However, the mechanism(s) underlying the beneficial effects remains to be elucidated. We hypothesized that dietary treatment with fish oil (FO) could mitigate LPS-induced activation of the hypothalamic-pituitary-adrenal (HPA) axis through inhibition of Toll-like receptor 4 and nucleotide-binding oligomerization domain protein signaling pathways. Twentyfour weaned pigs were used in a 2 × 2 factorial design, and the main factors consisted of diet (5% corn oil vs. 5% FO) and immunological challenge (saline vs. LPS). After 21 d of dietary treatment with 5% corn oil or FO diets, pigs were treated with saline or LPS. Blood samples were collected at 0 (preinjection), 2, and 4 h postinjection, and then pigs were humanely killed by intravenous injection of 40 mg/kg body weight sodium pentobarbital for tissue sample collection. FO led to enrichment of eicosapentaenoic acid and docosahexaenoic acid and total n-3 polyunsaturated fatty acids in hypothalamus, pituitary gland, adrenal gland, spleen, and thymus. FO decreased plasma adrenocorticotrophin and cortisol concentrations as well as mRNA expressions of hypothalamic corticotropin releasing hormone and pituitary proopiomelanocortin. FO also reduced mRNA expression of tumor necrosis factor-α in hypothalamus, adrenal gland, spleen, and thymus, and of cyclooxygenase 2 in hypothalamus. Moreover, FO downregulated the mRNA expressions of Toll-like receptor 4 (TLR4) and its downstream molecules, including cluster differentiation factor 14,myeloid differentiation factor 2, myeloid differentiation factor 88, interleukin- 1 receptor-associated kinase 1, tumor necrosis factor-α receptor-associated factor 6, and nuclear factor kappa-light-chainenhancer of activated B cells p65, and also decreased the mRNA expressions of nucleotide-binding oligomerization domain 1, nucleotide-binding oligomerization domain 2, and their adaptor molecule receptor-interacting serine/threonine-protein kinase 2. These results suggested that FOattenuates the activation of the HPA axis induced by LPS challenge. The beneficial effects of FO on the HPA axis may be associated with decreasing the production of brain or peripheral proinflammatory cytokines through inhibition of TLR4 and nucleotide-binding oligomerization domain protein signaling pathways. © 2013 American Society for Nutrition.
语种：
英文
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Fish Oil Increases Muscle Protein Mass and Modulates Akt/FOXO, TLR4, and NOD Signaling in Weanling Piglets After Lipopolysaccharide Challenge
作者：
Liu, Yulan* ;Chen, Feng;Odle, Jack;Lin, Xi;Zhu, Huiling;...
期刊：
The Journal of Nutrition ,2013年143(8):1331-1339 ISSN：0022-3166
通讯作者：
Liu, Yulan
作者机构：
[Liu, Yulan; Shi, Haifeng; Hou, Yongqing; Chen, Feng; Zhu, Huiling] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan, Peoples R China.;[Odle, Jack; Lin, Xi] N Carolina State Univ, Dept Anim Sci, Lab Dev Nutr, Raleigh, NC 27695 USA.;[Yin, Jingdong] China Agr Univ, Coll Anim Sci & Technol, State Key Lab Anim Nutr, Beijing 100094, Peoples R China.
通讯机构：
[Liu, Yulan] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan, Peoples R China.
摘要：
Proinflammatory cytokines play a key role in the pathophysiology of muscle atrophy. In addition, n3 polyunsaturated fatty acids (PUFAs) exert an inhibitory effect on proinflammatory cytokines affecting many inflammatory diseases. We hypothesized that dietary supplementation of fish oil could attenuate lipopolysaccharide (LPS)-induced muscle atrophy.Weanling pigswere used in a 2 × 2 factorial design and themain factors included diet (5% corn oil or 5%fish oil) and immunological challenge (LPS or saline). After 21 d of treatment with either fish oil or corn oil, pigs received an i.p. injection of either saline or LPS. At 4 h postinjection, blood and muscle samples were obtained. Fish oil led to enrichment of eicosapentaenoic acid, docosahexaenoic acid, and total n3 PUFAs in muscles. Fish oil increased muscle protein mass, indicated by a higher protein:DNA ratio in gastrocnemius and longissimus dorsi (LD) muscles. In addition, fish oil increased Akt1 mRNA abundance and decreased Forkhead Box O (FOXO) 1 and FOXO4 mRNA abundance. Fish oil also increased phosphorylation of Akt and FOXO1 in gastrocnemius and LD muscles. Fish oil decreased the mRNA abundance of muscle atrophy F-box (MAFbx) and muscle RING finger 1 in gastrocnemius and LD muscles. Moreover, fish oil reduced the plasma tumor necrosis factor (TNF) a, muscle TNFa, and prostaglandin E2 concentrations, and muscle TNFa and cyclooxygenase 2 (COX2) mRNA abundance. Finally, fish oil downregulated the mRNA abundance ofmuscle toll-like receptor (TLR4) and its downstream signalingmolecules [myeloid differentiation factor 88 (MyD88), TNFa receptor-associated factor 6 (TRAF6), and NF-kB p65], and nucleotide-binding oligomerization domain protein (NOD1), NOD2, and their adaptor molecule [receptor-interacting serine/threonine-protein kinase 2 (RIPK2)]. These results indicate fish oil may suppress muscle proinflammatory cytokine production via regulation of TLR and NOD signaling pathways and therefore improve muscle protein mass, possibly through maintenance of Akt/FOXO signaling. © 2013 American Society for Nutrition.
语种：
英文
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鱼油对脂多糖刺激仔猪肠黏膜能量代谢的影响
作者：
王秀英;刘玉兰;李藏兰;陈逢;朱惠玲;...
期刊：
中国畜牧杂志 ,2013年49(23):22-25 ISSN：0258-7033
作者机构：
1. 武汉轻工大学动物营养与饲料科学湖北省重点实验室;2. 中国农业大学动物科技学院;3. 福建高龙实业有限公司;[李藏兰] 中国农业大学;[丁斌鹰; 侯永清; 李爽; 王秀英; 朱惠玲; 陈逢; 刘玉兰; 石海峰] 武汉轻工大学
关键词：
鱼油;脂多糖;肠黏膜;能量代谢;仔猪
摘要：
试验研究鱼油对脂多糖(LPS)刺激仔猪肠黏膜能量代谢的影响。选用32头(28±3)d(8.91±0.74)kg的杜洛克×长白×大白仔猪,采用2×2因子设计,主因子包括日粮处理(5%鱼油或5%玉米油)和免疫应激(注射LPS或生理盐水),试验期19 d。试验第19天,每日粮组一半的猪注射100μg/kg BW的LPS,另一半注射生理盐水作对照。注射LPS 4 h后屠宰仔猪,取小肠样品待测。结果表明:鱼油对肠黏膜能量代谢的影响与LPS刺激不存在显著的互作关系;LPS刺激导致结肠三磷酸腺苷(ATP)水平显著下降(P<0.05);鱼油显著提高了结肠三磷酸腺苷(ATP)、一磷酸腺苷(AMP)、腺苷酸池(TAN)水平(P<0.05),对结肠AMP/ATP比值有提高的趋势(P<0.10),鱼油也显著降低了结肠能荷(EC)水平(P<0.05);鱼油和LPS刺激对空肠和回肠能量代谢指标均无显著影响。以上研究结果显示,鱼油可以缓解LPS应激引起的仔猪结肠黏膜能量代谢障碍,从而保护肠黏膜屏障。
语种：
中文
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Fish oil attenuates liver injury caused by LPS in weaned pigs associated with inhibition of TLR4 and nucleotide-binding oligomerization domain protein signaling pathways:
作者：
Chen, Feng;Liu, Yulan* ;Zhu, Huiling;Hong, Yu;Wu, Zhifeng;...
期刊：
INNATE IMMUNITY ,2013年19(5):504-515 ISSN：1753-4259
通讯作者：
Liu, Yulan
作者机构：
[Liu, Yulan; Wu, Zhifeng; Li, Quan; Hou, Yongqing; Chen, Feng; Hong, Yu; Ding, Binying; Yi, Dan; Zhu, Huiling; Chen, Hongbo] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
通讯机构：
[Liu, Yulan] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
关键词：
Fish oil;lipopolysaccharide;weaned pigs;liver injury;pro-inflammatory cytokines;Toll-like receptor 4;nucleotide-binding oligomerization domain protein
摘要：
This study evaluated whether fish oil exerted a hepatoprotective effect in a LPS-induced liver injury model via regulation of TLR4 and nucleotide-binding oligomerization domain protein (NOD) signaling pathways. Twenty-four piglets were used in a 2 × 2 factorial design, and the main factors included diet (5% corn oil or 5% fish oil) and immunological challenge (LPS or saline). Fish oil resulted in enrichment of eicosapentaenoic acid, docosahexaenoic acid and total (n-3) polyunsaturated fatty acids in liver. Less severe liver injury was observed in pigs fed fish oil, as evidenced by improved serum biochemical parameters and less severe histological liver damage. In addition, higher expression of liver tight junction proteins, and lower hepatocyte proliferation and higher hepatocyte apoptosis were observed in pigs fed fish oil. The improved liver integrity in pigs fed fish oil was concurrent with reduced hepatic mRNA expression of TLR4, myeloid differentiation factor 88, IL-1 receptor-associated kinase 1 and TNF-α receptor-associated factor 6, and NOD1, NOD2 and receptor-interacting serine/threonine-protein kinase 2, as well as reduced hepatic protein expression of NF-κB p65, leading to reduced hepatic pro-inflammatory mediators. These results indicate that fish oil improves liver integrity partially via inhibition of TLR4 and NOD signaling pathways under an inflammatory condition. © 2013 The Author(s).
语种：
英文
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Effect of l-arginine on intestinal mucosal immune barrier function in weaned pigs after Escherichia coli LPS challenge:
作者：
Zhu, H. L.;Liu, Y. L.* ;Xie, X. L.;Huang, J. J.;Hou, Y. Q.
期刊：
INNATE IMMUNITY ,2013年19(3):242-252 ISSN：1753-4259
通讯作者：
Liu, Y. L.
作者机构：
[Zhu, H. L.; Liu, Y. L.; Hou, Y. Q.; Xie, X. L.; Huang, J. J.] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
通讯机构：
[Liu, Y. L.] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
关键词：
Lipopolysaccharide;arginine;intestinal mucosal immune barrier function;weaned pigs
摘要：
The effects of l-arginine (Arg) supplementation on intestinal mucosal immune barrier function in weaned pigs after Escherichia coli LPS challenge were evaluated. Twenty-four weaned pigs were allotted to four treatments including: (i) non-challenged control; (ii) LPS-challenged control; (iii) LPS + 0.5% Arg; and (iv) LPS + 1.0% Arg. On d 16, pigs in the LPS, LPS + 0.5% Arg and LPS + 1.0% Arg groups were challenged by injection with 100 μg/kg of body mass LPS, whereas the control group were given sterile saline. At 48 h post-challenge, all pigs were sacrificed for evaluation of small intestinal morphology and mucosal immune barrier function. In the jejunum and ileum, LPS caused villous atrophy and intestinal morphology disruption, whereas 0.5% or 1.0% Arg supplementation mitigated villus atrophy and intestinal morphology impairment caused by LPS challenge. Arg (0.5%) supplementation increased the numbers of IgA-secreting cells, CD8+ and CD4+ T cells in the ileum (P < 0.05). Arg supplementation prevented the elevation of mast cell numbers induced by LPS challenge (P < 0.05). Dietary supplementation of Arg caused a decreased lymphocyte apoptosis of Peyer's patches in pigs challenged by LPS (P < 0.05). These results indicated that Arg supplementation protects and enhances intestinal mucosal immune barrier function and maintains intestinal integrity in weaned pigs after E. coli LPS challenge. © The Author(s) 2012 Reprints and permissions: sagepub.co.uk/journalsPermissions.nav.
语种：
英文
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Effects of Oral Administration of Spermine on the Development of Small Intestine and Growth Performance of Weaned Pigs
作者：
Kang, Ping;Wang, Meng;Hou, Yongqing* ;Yin, Yulong;Ding, Binying;...
期刊：
Journal of Animal and Veterinary Advances ,2012年11(15):2782-2787 ISSN：1680-5593
通讯作者：
Hou, Yongqing
作者机构：
[Liu, Yulan; Kang, Ping; Hou, Yongqing; Wang, Lei; Qiu, Yinsheng; Wang, Meng; Yin, Yulong; Ding, Binying; Yi, Dan; Zhu, Huiling] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Yin, Yulong] Chinese Acad Sci, Inst Subtrop Agr, Key Lab Agroecol Proc Subtrop Reg, Changsha 410125, Hunan, Peoples R China.;[Gong, Joshua] Agr & Agri Food Canada, Guelph Food Res Ctr, Guelph, ON N1G 5C9, Canada.
通讯机构：
[Hou, Yongqing] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
关键词：
D-xylose;Growth performance;Intestinal morphology;Intestinal protein/DNA;Piglets;Spermine
摘要：
The effects of spermine on the development of small intestine and growth performance of newly weaned pigs were investigated. Thirty pigs were used and the treatment of spermine was through oral administration for the 1 st 3 days after weaning with the dose of 0, 300, 600, 900 or 1200 umol per pig daily. The administration of spermine improved Average Daily Feed Intake (ADFI) and Average Daily Gam (ADG) in the periods 0-14 (p<0.05) but hadno significant effects on the ratio of Feed to Gain (F/G) and protein/DNA contents of the duodenal or ileal mucosa. The protein/DNA content of the jejunum was significantly increased at the doses of 300 and 600 umol day"1 spermine but was declined at the higher doses. The villus height in the duodenum (p<0.01) and ileum (p<0.05) increased with an increase in spermine doses. The crypt depth was reduced by spermine in the jejunum and the ratio of villus height/crypt depth was increased by spermine in the duodenum, jejunum and ileum (p<0.05). Additionally, the treatment of spermine at tested doses showed no effect on D-xylose absorption by the small intestine. These results indicate that oral administration of spermine for 3 days after weaning increased ADG and ADFI, enhanced the development of the small intestinal mucosa and improved intestinal morphology. © Medwell Journals, 2012.
语种：
英文
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Protective effects of N-acetylcysteine on intestinal functions of piglets challenged with lipopolysaccharide
作者：
Hou, Yongqing* ;Wang, Lei;Zhang, Wei;Yang, Zhenguo;Ding, Binying;...
期刊：
Amino Acids ,2012年43(3):1233-1242 ISSN：0939-4451
通讯作者：
Hou, Yongqing
作者机构：
[Liu, Yulan; Zhang, Wei; Hou, Yongqing; Yang, Zhenguo; Wang, Lei; Qiu, Yinsheng; Ding, Binying; Zhu, Huiling] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.
通讯机构：
[Hou, Yongqing] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
关键词：
N-acetylcysteine;Intestinal functions;Piglets;Lipopolysaccharide
摘要：
The neonatal small intestine is susceptible to damage by endotoxin, but effective methods for prevention and treatment are lacking. N-acetylcysteine (NAC) is a widely used precursor of L-cysteine for animal cells and plays an important role in protecting cells against oxidative stress. This study was conducted with the lipopolysaccharide (LPS)-challenged piglet model to determine the effects of NAC on intestinal function. Eighteen piglets were randomly allocated into control, LPS and LPS + NAC groups. The control and LPS groups were fed a corn- and soybean meal-based diet, and the LPS + NAC group was fed the basal diet +500 mg/kg NAC. On days 10, 13 and 20 of the trial, the LPS and LPS + NAC groups received intraperitoneal administration of LPS (100 lg/kg BW), whereas the control piglets received saline. On day 20 of the trial, D-xylose (0.1 g<kg BW) was orally administrated to all piglets 2 h after LPS or saline injection, and blood samples were collected 1 h thereafter. One hour blood xylose test was used to measure intestinal absorption capacity and mucosal integrity, and diamine oxidase (DAO) was used as a marker of intestinal injury. On day 21 of the trial, pigs were killed to obtain the intestinal mucosa. Compared to the control, LPS challenge reduced (P<0.05) the concentrations of D-xylose (a marker of intestinal absorption) in plasma, activities of DAO in the jejunal mucosa, the ratio of villus height to crypt depth in the jejunal mucosa, RNA/DNA and protein/DNA in the jejunal and ileal mucosae, while increasing (P<0.05) DAO activity in plasma and caspase-3 expression in the intestinal mucosa. The adverse effects of LPS were partially ameliorated (P<0.05) by NAC supplementation. Moreover, NAC prevented the LPS-induced decrease in claudin-1 and occludin expression in the jejunal and ileal mucosae. Collectively, these results indicate that dietary NAC supplementation alleviates the mucosal damage and improves the absorptive function of the small intestine in LPS-challenged piglets. © 2011 Springer-Verlag.
语种：
英文
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Dietary L-arginine supplementation alleviates liver injury caused by Escherichia coli LPS in weaned pigs:
作者：
Li, Quan;Liu, Yulan* ;Che, Zhengquan;Zhu, Huiling;Meng, Guoquan;...
期刊：
INNATE IMMUNITY ,2012年18(6):804-814 ISSN：1753-4259
通讯作者：
Liu, Yulan
作者机构：
[Che, Zhengquan; Liu, Yulan; Li, Quan; Meng, Guoquan; Hou, Yongqing; Chen, Feng; Yin, Yulong; Zhu, Huiling] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.;[Liu, Yulan; Yin, Yulong] Chinese Acad Sci, Inst Subtrop Agr, Changsha, Hunan, Peoples R China.
通讯机构：
[Liu, Yulan] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
关键词：
Arginine;weaned pigs;lipopolysaccharide;Toll-like receptor 4;nuclear factor-kappa B;liver injury
摘要：
This study was conducted to evaluate whether dietary supplementation with L-arginine (Arg) could attenuate Escherichia coli LPS-induced liver injury through the TLR4 signaling pathway in weaned pigs. Eighteen weaned pigs were allotted to three treatments: non-challenged control, LPS challenged control and LPS-+-0.5% Arg. On d 18, pigs were injected with LPS at 100-μg/kg of body weight (BW) or sterile saline. Blood samples were obtained at 4-h post-injection. Pigs were then sacrificed for the collection of liver samples. Arg supplementation (0.5%) alleviated liver morphological impairment, including hepatocyte caryolysis, karyopycnosis and fibroblast proliferation induced by LPS challenge; it mitigated the increase of serum aspartate aminotransferase and alkaline phosphatase activities induced by LPS (P-<-0.05); it prevented the increase of hepatic TNF-α, malondialdehyde contents and mast cell number induced by LPS administration (P-<-0.05); and it attenuated the elevation of hepatic NF-κB and TLR4-positive cell percentages (P-<-0.05). These results indicate that Arg supplementation has beneficial effects in attenuating hepatic morphological and functional injury induced by LPS challenge in piglets. Additionally, it is possible that the protective effects of Arg on the liver are associated with a decreased release of liver pro-inflammatory cytokines and free radicals through inhibiting TLR4 signaling. © The Author(s) 2012 Reprints and permissions: sagepub.co.uk/journalsPermissions.nav.
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英文
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Effect of three mycotoxin adsorbents on growth performance, nutrient retention and meat quality in broilers fed on mould-contaminated feed
作者：
Liu, Y. L.* ;Meng, G. Q.;Wang, H. R.;Zhu, H. L.;Hou, Y. Q.;...
期刊：
British Poultry Science ,2011年52(2):255-263 ISSN：0007-1668
通讯作者：
Liu, Y. L.
作者机构：
[Liu, Y. L.; Zhu, H. L.; Hou, Y. Q.; Ding, B. Y.; Wang, H. R.; Meng, G. Q.] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Wang, W. J.] S Cent Univ Nationalities, Coll Life Sci, Wuhan 430074, Peoples R China.
通讯机构：
[Liu, Y. L.] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
摘要：
1. A study was conducted to investigate the effects of an esterified glucomannan (EGM), a hydrated sodium calcium aluminosilicate (HSCAS) and a compound mycotoxin adsorbent (CMA) on performance, nutrient retention and meat quality in broilers fed on mould-contaminated feed. Mouldcontaminated diets were prepared by replacing half of the non-contaminated maize in the basal diets with mould-contaminated maize, which contained 450·6 mg/kg of aflatoxin B1, 68·4 mg/kg of ochratoxin A and 320·5 mg/kg of T-2 toxin. 2. The mould-contaminated diet significantly decreased body weight gain (BWG) between 10 and 21d, feed intake (FI) between 35 and 42d, the apparent retention of crude lipid and phosphorus, and the lightness (L*) value of breast and thigh muscle. It also significantly increased the redness (a*) and yellowness (b*) value in breast muscle and the b* value in thigh muscle. 3. The addition of 0 2% HSCAS significantly increased FI between 35 and 42d and the apparent retention of phosphorus. Supplementation with 0 1% CMA in the contaminated diet significantly improved BWG from 10 to 21d, and increased FI from 35 to 42d and from 10 to 42d. CMA also significantly increased the apparent retention of crude lipid, crude protein, ash and phosphorus. All three mycotoxin-adsorbent treatments significantly improved the L* values of breast and thigh muscle when compared with the mould-contaminated group. Supplementation with 0·1% CMA in the contaminated diet significantly decreased b* value and improved tenderness in thigh muscle. 0·05% EGM significantly decreased b* value of thigh muscle compared to mould-contaminated group. 4. The results indicated that mycotoxins in contaminated feed retard growth, nutrient retention and meat quality, whereas the addition of 0·05% EGM, 0·2% HSCAS or 0·1% CMA prevents the adverse effects of mycotoxins to varying extents, with 0 1% CMA being the most effective adsorbent treatment. © 2011 British Poultry Science Ltd.
语种：
英文
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Alpha-ketoglutarate and intestinal function
作者：
Hou, Yongqing* ;Wang, Lei;Ding, Binying;Liu, Yulan;Zhu, Huiling;...
期刊：
Frontiers in Bioscience-Landmark ,2011年16(3):1186-1196 ISSN：2768-6701
通讯作者：
Hou, Yongqing
作者机构：
[Liu, Yulan; Kang, Ping; Hou, Yongqing; Liu, Jian; Wang, Lei; Li, Yongtang; Ding, Binying; Zhu, Huiling] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;Chinese Acad Sci, Inst Subtrop Agr, Changsha 410125, Peoples R China.;China Agr Univ, State Key Lab Anim Nutr, Beijing 100193, Peoples R China.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.
通讯机构：
[Hou, Yongqing] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
关键词：
Alpha-ketoglutarate;Gut function;Intestine;Lipopolysaccharide;Piglets;Review
摘要：
Alpha-ketoglutarate (AKG) is an intermediate of the Krebs cycle which bridges amino acid metabolism with glucose oxidation in animals. Of particular interest is the conversion of AKG into glutamate by mitochondrial glutamate dehydrogenase in the gastrointestinal tract where glutamate has multiple physiological functions (including regulation of cell function, neurotransmission, and gastric emptying). Additionally, AKG stimulates the initiation of catabolism of branched-chain amino acids (BCAA) via BCAA transaminase in enterocytes. Oxidation of AKG also provides large amounts of ATP and modulates cellular redox state in the small intestine. Translating the basic research into practice, results of recent studies indicate that dietary supplementation with AKG alleviates oxidative stress and injury in intestinal mucosal cells, while improving intestinal mucosal integrity and absorption of nutrients in endotoxin-challenged pigs. The beneficial effects of AKG are associated with increased activation of the mTOR signaling pathway and net protein synthesis. Thus, AKG is a novel and promising supplement in diets to improve intestinal health in animals and possibly humans.
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The protective effects of different mycotoxin adsorbents against blood and liver pathological changes induced by mold-contaminated feed in broilers
作者：
Che, Zhengquan;Liu, Yulan* ;Wang, Huirong;Zhu, Huiling;Hou, Yongqing;...
期刊：
Animal Bioscience ,2011年24(2):250-257 ISSN：2765-0189
通讯作者：
Liu, Yulan
作者机构：
[Che, Zhengquan; Liu, Yulan; Hou, Yongqing; Ding, Binying; Wang, Huirong; Zhu, Huiling] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
通讯机构：
[Liu, Yulan] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
关键词：
Che, Zhengquan;Liu, Yulan;Wang, Huirong;Zhu, Huiling;Hou, Yongqing;Ding, Binying;Mycotoxin;Mycotoxin Adsorbents;Blood Parameters;Liver Morphology;Broilers
摘要：
An experiment was conducted to determine the effects of different mycotoxin adsorbents including esterified glucomannan (EGM), hydrated sodium calcium aluminosilicate (HSCAS) and compound mycotoxin adsorbent (CMA) on performance, blood parameters, and liver pathological changes in broilers fed mold-contaminated feed. Two hundred and forty 10-day-old broilers were randomly assigned to one of the five dietary treatments including: I) control diet; ii) mold-contaminated diet; iii) moldcontaminated diet+0.05% EGM; iv) mold-contaminated diet+0.2% HSCAS; v) mold-contaminated diet+0.1% CMA. At 35-days-old, blood and liver tissue samples were collected for analysis. 0.1% CMA improved ADG and ADFI during 10-42 d compared to the moldcontaminated group (p<0.05). The mold-contaminated diet increased total white blood cell (WBC) number, haemoglobin (Hgb) concentration, hematocrit (Hct) level, serum aspartate aminotransferase (AST) and ?-glutamyl transferase (GGT) activities, and decreased red blood cell (RBC) number and serum globulin (GLB) and urea nitrogen (BUN) concentrations (p<0.05). The three mycotoxin adsorbents alleviated the alteration of RBC, WBC, Hgb and AST caused by the mold-contaminated diet. Furthermore, 0.1% CMA increased GLB concentration and decreased Hct level and GGT activity (p<0.05). Liver superoxide dismutase (SOD) activity was reduced, and myeloperoxidase (MPO) activity was increased by the mold-contaminated diet (p<0.05). Both EGM and HSCAS prevented the increase of MPO activity (p<0.05). Liver lesion, including severe vacuolar degeneration of hepatocytes, was observed in chicks fed the mold-contaminated diet. 0.05% EGM prevented these effects except for biliary hyperplasia and mild vacuolar degeneration. 0.2% HSCAS showed medium vacuolar degeneration of hepatocytes. Liver of broilers fed 0.1% CMA revealed a mild vacuolar degeneration. These results indicate that a mold-contaminated diet results in adverse effects on blood parameters and liver morphology. 0.05% EGM and 0.2% HSCAS partially alleviated the adverse effects. However, 0.1% CMA almost completely ameliorated the adverse effects.
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英文
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Effects of α-ketoglutarate on energy status in the intestinal mucosa of weaned piglets chronically challenged with lipopolysaccharide
作者：
Hou, Yongqing* ;Yao, Kang;Wang, Lei;Ding, Binying;Fu, Dabo;...
期刊：
British Journal of Nutrition ,2011年106(3):357-363 ISSN：0007-1145
通讯作者：
Hou, Yongqing
作者机构：
[Liu, Yulan; Kang, Ping; Hou, Yongqing; Liu, Jian; Wang, Lei; Li, Yongtang; Ding, Binying; Fu, Dabo; Zhu, Huiling] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Yao, Kang; Yin, Yulong] Chinese Acad Sci, Inst Subtrop Agr, Changsha 410125, Hunan, Peoples R China.;[Yao, Kang; Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.;[Wu, Guoyao] China Agr Univ, State Key Lab Anim Nutr, Beijing 100193, Peoples R China.
通讯机构：
[Hou, Yongqing] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
关键词：
alpha-Ketoglutarate;Intestinal mucosae;AMP-activated protein kinase signalling;Piglets;Lipopolysaccharide
摘要：
The present study determined whether α-ketoglutarate (AKG) might affect the expression of AMP-activated protein kinase (AMPK) and energy status in the intestinal mucosa of piglets challenged with Escherichia coli lipopolysaccharide (LPS). A total of eighteen piglets (weaned at 21 d of age) were allocated to one of three treatments: (1) non-challenged (control); (2) LPS-challenged (LPS); (3) LPS+1 % AKG (LPS+AKG). Piglets in the control and LPS groups were fed a maize-and soyabean meal-based diet, and the LPS+AKG group was fed the basal diet supplemented with 1 % AKG. On days 10, 12, 14 and 16 of the trial, piglets in the LPS and LPS+AKG groups were challenged with LPS (80 Î1/4g/kg body weight), whereas piglets in the control group received the same volume of sterile saline. Pigs were euthanised 24 h after the last administration of LPS or saline to obtain intestinal mucosae for biochemical analysis. Compared with the control group, LPS administration decreased (P < 0•05) the oxidation of AKG, oleic acid, glutamine and glucose in enterocytes, decreased concentrations of ATP in the duodenal and jejunal mucosae and decreased adenylate energy charge (AMP:ATP ratio) in the jejunal and ileal mucosae. Additionally, LPS treatment reduced (P < 0•05) mucosal concentrations of phosphorylated AMPK in the jejunum and ileum as well as acetyl-CoA carboxylase in all segments of the small intestine. The adverse effects of LPS were reversed by AKG. Collectively, these results indicate that dietary supplementation with 1 % AKG beneficially modulates the AMPK signalling pathway to improve energy status in the small intestine of LPS-challenged piglets. © 2011 The Authors.
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Effects of astragalus polysaccharides, achyranthes bidentata polysaccharides, and acantbepanax senticosus saponin on the performance and immunity in weaned pigs
作者：
Kang, P.;Xiao, H. L.;Hou, Y. Q.* ;Ding, B. Y.;Liu, Y. L.;...
期刊：
Animal Bioscience ,2010年23(6):750-756 ISSN：2765-0189
通讯作者：
Hou, Y. Q.
作者机构：
[Hou, Y. Q.; Liu, Y. L.; Zhu, H. L.; Ding, B. Y.; Hu, Y.; Hu, Q. Z.; Kang, P.; Yin, Y. L.; Xiao, H. L.] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Hou, Y. Q.] Chinese Acad Sci, Inst Subtrop Agr, Changsha 410125, Hunan, Peoples R China.
通讯机构：
[Hou, Y. Q.] C;Chinese Acad Sci, Inst Subtrop Agr, Changsha 410125, Hunan, Peoples R China.
关键词：
Astragalus Polysaccharides;Achyranthes bidentata Polysaccharides;Acantbepanax senticosus Saponin;Growth Performance;Immunity;Weaned Pigs
摘要：
Two trials were conducted to study the effects of two Chinese herbal polysaccharides, Astragalus polysaccharides (APS) and Achyranthes bidentata polysaccharides (ABPS), and one Chinese herbal saponin, Acantbepanax senticosus saponin (ASS), on the immunity and growth performance of weaned pigs. Experiment 1 was a 14-day growth assay, in which 32 weaned pigs were randomly allocated to one of four dietary treatments: i) 0.05% talcum powder control; ii) 0.05% APS; iii) 0.05% mixture of APS and ASS in a 1:1 ratio by weight; and iv) 0.05% mixture of APS, ASS, and ABPS in a ratio of 1:1:1 by weight. Blood samples were collected on day 14 to determine plasma parameters. Feed intake, body weight gain, and feed efficiency were also determined. Experiment 2 was a 21-day immunity assay, in which 16 weaned pigs were randomly allotted to one of two dietary treatments: i) 0.05% talcum powder control; and ii) 0.05% mixture of APS and ASS in a 1:1 ratio by weight. On day 21, pigs were challenged with lipopolysaccharide (LPS) and 3 h later blood samples were collected and analyzed for lymphocyte proliferation as well as interleukin 6 (IL-6), insulin-like growth factor 1 (IGF-I), growth hormone (GH), and Cortisol levels. In Experiment 1, feeding Chinese herbal polysaccharides and saponin increased growth performance of the pigs. The effects of the mixture of APS and ASS were especially notable, as there was a significant improvement in growth performance compared with the control (p<0.05). The plasma concentration of immunoglobulin G (IgG), nitric oxide (NO), and nitric oxide synthase (NOS) were increased in all treatments groups, with the mixture of APS and ASS increasing the level of IgG and NOS significantly (p<0.05), compared with the control. There was no difference in the NO level between the control and treatment groups (p>0.05). In Experiment 2, Chinese herbal polysaccharides and saponin showed immunostimulating effects. The level of Cortisol, GH, and IGF-I were significantly increased (p>0.05), and the level of IL-6 showed a significant decrease (p<0.05) in the APS and ASS treatment after the LPS challenge. The mixture of APS and ASS could stimulate the blood lymphocyte proliferation significantly whether the LPS was injected or not (p<0.05). These results show that Chinese herbal extracts can improve growth performance and stimulate immunity of weaned pigs. A mixture of APS and ASS, compared with APS alone, could be a new kind of immunostimulant for weaned pigs, which could result in greater positive effects on their growth performance and immunity.
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英文
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脯氨酸对脂多糖多次刺激的仔猪生长性能及血液生化指标的影响
作者：
张丽丽;胡尧;侯永清;丁斌鹰;王蕾;...
期刊：
饲料工业 ,2010年31(13):11-13 ISSN：1001-991X
作者机构：
武汉工业学院动物科学与营养工程学院,湖北省动物营养与饲料科学重点实验室,430023;[丁斌鹰; 张丽丽; 侯永清; 胡尧; 王蕾; 朱惠玲; 刘玉兰] 武汉工业学院
关键词：
脯氨酸;脂多糖;生长性能;血液生化指标;仔猪
摘要：
试验研究了脯氨酸(pmline,PRO)对脂多糖(LPS)多次刺激的仔猪生长性能、血液生化指标的影响.选取来源一致、体重相近[(9.13±0.85)kgl的健康断奶仔猪36头,采用2×3因子试验设计.设免疫应激和PRO添加量2个因子,其中免疫应激设2个水平:无应激(不注射LPS,注射相同剂量的生理盐水)和LPS刺激(腹膜注射100 μg/kgBW LPS);PRO添加量设3个水平:0.05%和1.0%.共6个处理组,每个处理6个重复,每重复1头仔猪,试验期20 d.试验前10 d,未进行LPS处理,于试验的第10、13和20 d对应激组仔猪腹膜注射100μg/kgBW LPS,无应激组仔猪注射相应剂量的生理盐水.试验第10、20 d空腹称重,并于注射LPS 3 h后前腔静脉采血,观测有关指标.试验结果显示:①LPS刺激降低了仔猪生长性能、提高了血浆中尿素氮,对过氧化氢酶(CAT)、丙二醛(MDA)和超氧化物歧化酶(SOD)均无影响.②日粮添加PRO可以显著降低血浆BUN和MDA,提高血浆SOD,以0.5%的效果较好.在本次试验条件下,LPS刺激显著降低了仔猪的生长性能,提高了血浆中尿素氮水平,日粮添加0.5%的PRO可提高机体抗氧化能力和降低血浆中尿素氮水平;添加脯氨酸对LPS刺激导致的生长抑制没有缓解作用.
语种：
中文
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Effects of rosiglitazone, an agonist of the peroxisome proliferator- activated receptor γ, on intestinal damage induced by Escherichia coli lipopolysaccharide in weaned pigs
作者：
Fan, Wei;Liu, Yulan* ;Wu, Zhifeng;Hong, Yu;Zhu, Huiling;...
期刊：
AMERICAN JOURNAL OF VETERINARY RESEARCH ,2010年71(11):1331-1338 ISSN：0002-9645
通讯作者：
Liu, Yulan
作者机构：
[Liu, Yulan; Wu, Zhifeng; Hou, Yongqing; Fan, Wei; Han, Jie; Hong, Yu; Yin, Yulong; Zhu, Huiling] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei Province, Peoples R China.;[Yin, Yulong] Chinese Acad Sci, Inst Subtrop Agr, Changsha 410125, Hunan, Peoples R China.
通讯机构：
[Liu, Yulan] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei Province, Peoples R China.
摘要：
Objective - To determine whether rosiglitazone, an agonist of the peroxisome proliferator-activated receptor (PPAR) γ, could alleviate intestinal damage induced by Escherichia coli lipopolysaccharide (LPS) in weaned pigs. Animals - 18 weaned pigs (mean ± SD age, 28 ± 3 days). Procedures - Pigs were allocated to 3 treatments (6 pigs/treatment). Control pigs were injected IP with dimethyl sulfoxide and then injected 30 minutes later with sterile saline (0.9% NaCl) solution, LPS-treated pigs were injected IP with dimethyl sulfoxide and then injected 30 minutes later with LPS (100 μ g/kg, IP), and rosiglitazone plus LPS-treated pigs were injected with rosiglitazone (3 mg/kg, IP) and then injected 30 minutes later with LPS (100 μ g/kg, IP). Pigs were euthanized 3 hours after challenge exposure, and samples of the small intestines were collected for histologic, biochemical, and immunohistochemical examination. Results - Rosiglitazone alleviated LPS-induced intestinal damage, which was manifested as a lower crypt depth in the duodenum and a higher villus height-to-crypt depth ratio in the duodenum, jejunum, and ileum. Rosiglitazone also mitigated inhibition of crypt cell proliferation in the jejunum and ileum induced by LPS injection. Pretreatment with rosiglitazone significantly increased the number of cells that stained for PPAR? and significantly decreased the number of cells that stained for inducible nitric oxide synthase. Conclusions and Clinical Relevance - Rosiglitazone alleviated intestinal damage induced by LPS injection in weaned pigs. The protective effects of rosiglitazone on the intestines may be associated with inhibition of intestinal proinflammatory mediators, such as inducible nitric oxide synthase.
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英文
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Up-regulated expression of peroxisome proliferator-activated receptor γ in the hypothalamic–pituitary–adrenal axis of weaned pigs after Escherichia coli lipopolysaccharide challenge
作者：
Liu, Yu Lan* ;Shi, Jun Xia;Lu, Jing;Che, Zheng Quan;Zhu, Hui Ling;...
期刊：
VETERINARY JOURNAL ,2010年184(2):230-235 ISSN：1090-0233
通讯作者：
Liu, Yu Lan
作者机构：
[Shi, Jun Xia; Yin, Yu Long; Che, Zheng Quan; Hou, Yong Qing; Zhu, Hui Ling; Liu, Hong Ming; Liu, Yu Lan; Lu, Jing; Zhao, Sheng Jun; Ding, Bin Ying] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Yin, Yu Long] Chinese Acad Sci, Inst Subtrop Agr, Changsha 410125, Hunan, Peoples R China.
通讯机构：
[Liu, Yu Lan] W;Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
关键词：
Peroxisome proliferator-activated receptor gamma;Lipopolysaccharide;Hypothalamic-pituitary-adrenal axis;Pigs
摘要：
The expression of peroxisome proliferator-activated receptor gamma (PPAR gamma) was investigated in the hypothalamic-pituitary-adrenal (HPA) axis of weaned pigs after injection with 100 mu g/kg bodyweight Escherichia coli lipopolysaccharide (LPS) (n = 6) and control pigs injected with sterile saline (n = 6). LPS increased PPAR gamma mRNA and protein expression in the hypothalamus (23.8 and 3.1-fold relative to controls, respectively), pituitary gland (9.2 and 2.0-fold, respectively) and adrenal gland (3.5 and 2.3-fold, respectively) (P < 0.05). LPS also induced an increase in PPAR gamma immunohistochemical staining in the hypothalamus (1.3-fold), adenohypophysis (1.3-fold), adrenal cortex (1.4-fold) and adrenal medulla (1.6-fold) (P < 0.05). Concurrent with up-regulated expression of PPAR gamma, LPS increased the concentrations of plasma corticotrophin-releasing hormone (2.1-fold) and adrenocorticotrophin (1.4-fold) (P < 0.05). LPS also induced elevations of interleukin 6 and tumour necrosis factor alpha mRNA levels in the hypothalamus (4.0 and 3.2-fold, respectively), pituitary gland (20.7 and 5.1-fold, respectively) and adrenal gland (3.9 and 3.3-fold, respectively) (P < 0.05). PPAR gamma may play a role in the regulation of neuroendocrine responses associated with immunological stress in pigs. (C) 2009 Elsevier Ltd. All rights reserved.
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英文
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Dietary α-ketoglutarate supplementation ameliorates intestinal injury in lipopolysaccharide-challenged piglets
作者：
Hou, Yongqing* ;Wang, Lei;Ding, Binying;Liu, Yulan;Zhu, Huiling;...
期刊：
Amino Acids ,2010年39(2):555-564 ISSN：0939-4451
通讯作者：
Hou, Yongqing
作者机构：
[Liu, Yulan; Hou, Yongqing; Liu, Jian; Wang, Lei; Li, Yongtang; Ding, Binying; Zhu, Huiling] Wuhan Polytech Univ, Hubei key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.;[Wu, Xin; Yin, Yulong] Chinese Acad Sci, Inst Subtrop Agr, Changsha 410125, Hunan, Peoples R China.;[Wu, Guoyao] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.
通讯机构：
[Hou, Yongqing] W;Wuhan Polytech Univ, Hubei key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China.
关键词：
alpha-Ketoglutarate;Intestine;mTOR signaling;Piglets;Lipopolysaccharide
摘要：
Neonates are at increased risk for inflammatory bowel disease, but effective prevention and treatments are currently limited. This study was conducted with the lipopolysaccharide (LPS)-challenged piglet model to determine the effects of dietary supplementation with α-ketoglutarate (AKG) on the intestinal morphology and function. Eighteen 24-day-old pigs (weaned at 21 days of age) were assigned randomly to control, LPS, and LPS + AKG groups. The piglets in the control and LPS groups were fed a corn-and soybean meal-based diet, whereas the LPS + AKG group was fed the basal diet supplemented with 1% AKG. On days 10, 12, 14, and 16, piglets in the LPS and LPS + AKG groups received intraperitoneal administration of LPS (80 μg/kg BW), whereas piglets in the control group received the same volume of saline. On day 16, D-xylose was orally administrated to all pigs at the dose of 0.1 g/kg BW, 2 h after LPS or saline injection, and blood samples were collected 3 h thereafter. Twenty-four hours post-administration of LPS or saline, pigs were killed to obtain intestinal mucosae for analysis. Compared with the control group, LPS challenge reduced (P < 0.05) protein levels, the ratio of villus height to crypt depth, and the ratio of phosphorylated mTOR to total mTOR in duodenal, jejunal, and ileal mucosa. These adverse effects of LPS were attenuated (P > 0.05) by AKG supplementation. Moreover, AKG prevented the LPS-induced increase in intestinal HSP70 expression. Collectively, these novel results indicate that dietary supplementation with 1% AKG activates the mTOR signaling, alleviates the mucosal damage, and improves the absorptive function of the small intestine in LPS-challenged piglets. The findings not only help understand the mode of AKGs actions in the neonatal gut but also have important implications for infant nutrition under inflammatory conditions. © Springer-Verlag 2010.
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英文
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N-乙酰半胱氨酸对脂多糖应激仔猪生长性能及血浆生化指标的影响
作者：
胡尧;张丽丽;侯永清;丁斌鹰;朱惠玲;...
期刊：
动物营养学报 ,2010年22(4):1007-1011 ISSN：1006-267X
作者机构：
武汉工业学院,动物营养与饲料科学湖北省重点实验室,武汉,430023
关键词：
N-乙酰半胱氨酸;脂多糖;生长性能;表皮生长因子;仔猪
摘要：
本文旨在研究N-乙酰半胱氨酸(NAC)对脂多糖(LPS)应激仔猪生长性能及血浆生化指标的影响.选取来源一致、平均体重(11.58±0.26)kg的24头健康仔猪,随机分成2组,每组6个重复,每重复2头仔猪.试验分2个阶段,第1～9天对照组饲喂基础饲粮,试验组饲喂在基础饲粮中添加500mg/kg NAC.试验第10～20天分别将2个组同一重复猪中1头分出单独饲养,于第10天、第13天和第20天,1头仔猪腹膜下注射100mg/kg LPS,另一头仔猪注射等体积生理盐水.结果显示:1)饲粮中添加NAC可以缓解LPS刺激导致的日增重下降(P<0.05);2)饲粮中长时间添加NAC能极显著提高仔猪血浆谷胱甘肽水平(P<0.01),显著提高血浆超氧化物歧化酶活性(P<0.05),显著提高血浆表皮生长因子水平(P<0.05).由此可见,饲粮中添加NAC可缓解LPS应激导致的抑制仔猪生长.
语种：
中文
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罗格列酮和双酚A型环氧树脂对脂多糖应激断奶仔猪白细胞分类计数和血液生化指标的影响
作者：
刘玉兰;鲁晶;石君霞;侯永清;朱惠玲;...
期刊：
动物营养学报 ,2010年22(1):145-150 ISSN：1006-267X
作者机构：
[丁斌鹰; 侯永清; 石君霞; 鲁晶; 朱惠玲; 刘玉兰] 动物营养与饲料科学湖北省重点实验室
关键词：
罗格列酮;双酚A型环氧树脂;断奶仔猪;脂多糖应激
摘要：
试验研究了过氧化物酶体增殖物激活受体γ(PPARγ)的激动剂和抑制剂对脂多糖(LPS)应激断奶仔猪白细胞分类计数和血液生化指标的影响.选取24头健康断奶仔猪,分为4组,每组6个重复,每个重复1头猪.4个组分别为:对照组;LPS组;罗格列酮(ROS,PPARγ激动剂)+LPS组;双酚A型环氧树脂(BADGE,PPARγ抑制剂)+ROS+LPS组.注射LPS或生理盐水3 h后,前腔静脉采血待测.结果显示:1)ROS缓解了LPS导致的断奶仔猪血液白细胞总数和淋巴细胞数量的降低(P<0.05),BADGE阻断了ROS的缓解作用(P<0.05);2)ROS缓解了LPS导致的断奶仔猪血浆碱性磷酸酶活性的升高(P<0.05)及总蛋白、白蛋白和球蛋白含量的降低(P<0.10),BADGE阻断了ROS的缓解作用(P<0.10).结果表明,ROS在一定程度上缓解LPS应激导致的断奶仔猪白细胞总数和淋巴细胞数量的下降及部分血液生化指标的改变,而BADGE阻断了ROS的缓解作用.
语种：
中文
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