Glucose oxidase (GOX) holds significant application value in food, animal husbandry, and medical fields. However, its industrial use is limited by inherently insufficient thermostability and suboptimal catalytic efficiency. To address this challenge, a semi-rational design workflow was developed in this study, which combines multi-strategy computational screening with single-site saturation mutagenesis. Strategy I integrates molecular docking, co-evolutionary analysis, and consensus residue identification to enhance catalytic efficiency. Strate...
