作者机构：
[Zhang, Junqi; Yin, Wenhao; Jin, Shuqi; Deng, Wenwen; Wang, Xinping; Jiang, Qingyuan] State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, Hubei, 430042, China;[Yin, Wenhao] BravoVax Co., Ltd, Wuhan, Hubei, 430075, China;[Mao, Wuxiang] State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, Hubei, 430042, China. wxmao@hubu.edu.cn;[He, Ruyi] Pilot Base of Food Microbial Resources Utilization of Hubei Province, School of Life Science and Technology, Wuhan Polytechnic University, Wuhan, Hubei, 430023, China;[Qiao, Jie] Pilot Base of Food Microbial Resources Utilization of Hubei Province, School of Life Science and Technology, Wuhan Polytechnic University, Wuhan, Hubei, 430023, China. jieqiao@whpu.edu.cn
通讯机构：
[Wuxiang Mao; Yi Liu] S;[Jie Qiao] P;State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, China<&wdkj&>State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, China<&wdkj&>BravoVax Co., Ltd, Wuhan, China<&wdkj&>Pilot Base of Food Microbial Resources Utilization of Hubei Province, School of Life Science and Technology, Wuhan Polytechnic University, Wuhan, China<&wdkj&>State Key Laboratory of Esophageal Cancer Prevention & Treatment, Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou, China
摘要：
The CRISPR/Cas12a system has revolutionized molecular diagnostics, yet the direct detection of RNA, particularly those with complex structures, remains a significant challenge. Here, we present an updated SCas12a system, termed SCas12aV2, which enables precise, amplification-free detection of highly structured RNA molecules. By optimizing the length of scaffold RNA, targeting asymmetric structures, and utilizing dsDNA-ssDNA hybrid activators, we have significantly reduced steric hindrance in the detection system, thereby markedly enhancing both sensitivity and kinetics compared to traditional DNA activators. The SCas12aV2 assay achieves a detection limit of 246 aM for pooled activators and 10 pM for single-site targeting, demonstrating high specificity for single nucleotide polymorphisms (SNPs). It successfully identifies viable bacteria and SARS-CoV-2 infections in clinical samples. The assay is versatile and can be applied to various Cas12a orthologs, including thermostable CtCas12a. This work advances molecular diagnostics by improving the accuracy and efficiency of RNA detection. This study presents an updated split Cas12a system that enables amplification-free detection of the RNA with complex structures, thereby advancing point-of-care diagnostics.
期刊：
Current Microbiology,2025年82(4):183 ISSN：0343-8651
通讯作者：
Yamin Geng
作者机构：
[Hehong Wei; Ying Yao; Bingqing Ding] College of Energy and Environmental Engineering, Hebei University of Engineering, Handan, 056038, China;[Na Gao] Key Laboratory of Freshwater Aquaculture and Enhancement of Anhui Province, Fisheries Research Institute, Anhui Academy of Agricultural Sciences, Hefei, 230031, China;[Yaqi Liu] Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture (CAS), Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China;[Yaqi Liu] University of Chinese Academy of Sciences, Beijing, 100049, China;[Jingcheng Dai; Yangyang Sun] School of Life Science and Technology, Wuhan Polytechnic University, Wuhan, 430023, China
通讯机构：
[Yamin Geng] C;College of Energy and Environmental Engineering, Hebei University of Engineering, Handan, China
摘要：
A novel bacterial strain, designated Dechloromonas aquae ZY10(T), was isolated from the aquaculture water of grass carp. The colonies exhibited diameters ranging from approximately 1 to 3mm and were characterized by a creamy-white coloration, circular shape, smooth texture, translucency, and a convex profile. The cells were facultatively anaerobic and motile, utilizing a single polar flagellum for movement. They were rod-shaped, reproduced through binary fission, and were identified as Gram-negative, oxidase-negative, and catalase-positive. Optimal growth was observed between 37 and 40°C, within a pH range of 7.0-9.0, and at a NaCl concentration of 0% (w/v). The respiratory quinone was ubiquinone-8 (97.6%) and ubiquinone-7 (2.4%), and the major polar lipids were phosphatidylethanolamine (PE), aminophospholipid (APL), diphosphatidylglycerol (DPG), and phosphatidylglycerol (PG). Phylogenetic analysis based on 16S rRNA gene sequences suggested that ZY10(T) formed a lineage within the genus Dechloromonas and showed the highest 16S rRNA gene sequence similarity to "D. hankyongensis" XY25(T) (96.5%), followed by D. denitrificans ED1(T) (96.4%), D. hortensis MA-1(T) (96.1%) and D. agitata CKB(T) (96.0%) and Azonexus. caeni Slo-05(T) (95.5%). The predominant fatty acids of ZY10(T) were summed feature 3 (comprising C(16:1) ω6c and/or C(16:1) ω7c) and C(16:0). The whole genome of ZY10(T) was 3,568,927bp in size, including 3,275 protein-coding genes, 72 tRNA genes, and 15 rRNA genes, and the genomic DNA G + C content was 62.0mol%. The orthologous average nucleotide identity, average amino acid identity, and digital DNA-DNA hybridization values between ZY10(T) and other species within the family Azonexaceae were 78.0-81.5%, 71.8-73.9%, and 20.3-23.0%, respectively. Therefore, based on phenotypic, chemotaxonomic, and phylogenetic analyses, the isolated ZY10(T) (= MCCC 1K08699(T) = KCTC 72749(T)) is proposed as type strain of the novel species Dechloromonas aquae sp. nov.
期刊：
International Journal of Biological Macromolecules,2025年293:139340 ISSN：0141-8130
通讯作者：
Zhang, Rui;He, JR
作者机构：
[Chen, Ming; Pei, Xun; Yin, Jinjing; Zhang, Rui; Xiong, Sihui; Wu, Muci; He, Jingren] Wuhan Polytech Univ, Natl R&D Ctr Se rich Agr Prod Proc, Hubei Engn Res Ctr Deep Proc Green Se Rich Agr Pro, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.;[Oliveira, Helder; Mateus, Nuno] Univ Porto, Fac Sci, Dept Chem & Biochem, REQUIMTE LAQV, P-4169007 Porto, Portugal.;[Ye, Shuxin] Yun Hong Grp Co Ltd, Wuhan 430206, Hubei, Peoples R China.;[Zhang, Rui; He, Jingren] Wuhan Polytech Univ, Key Lab Deep Proc Major Grain & Oil, Hubei Key Lab Proc & Transformat Agr Prod, Minist Educ, Wuhan 430023, Peoples R China.;[He, Jingren; Zhang, Rui] Wuhan Polytech Univ, 36 Huanhu Middle Rd, Wuhan 430023, Peoples R China.
通讯机构：
[He, JR ; Zhang, R] W;Wuhan Polytech Univ, 36 Huanhu Middle Rd, Wuhan 430023, Peoples R China.
关键词：
Antioxidant activity;Antiproliferative capacity;Phenolics;Protein;Purple rice (Oryza sativa L.)
摘要：
Purple rice ( Oryza sativa L.) is a rich in endogenous phenolics and proteins. The naturally occurring interactions between phenolic compounds and proteins have been shown to have beneficial effects on human health. In this study, four protein fractions of purple rice (albumin, prolamin, globulin, and glutelin) were extracted, and both protein-free and protein-bound phenolics (PFP and PBP) were isolated from each protein fraction. The major phenolics compounds identified in different protein fraction included protocatechuic acid, vanillic acid, and ferulic acid. Additionally, the PFP in the albumin fraction exhibited the highest number of anthocyanin glycosides (7 types) among all phenolic compounds, while the remaining compounds were identified only as cornflower-3-glucoside and paeoniflorin-3-glucoside. Moreover, the in vitro antioxidant activity and cancer cell inhibitory effects of PFP and PBP in various protein fraction were investigated using chemiluminescence and cellular assays. The results demonstrated that the inhibitory effect of H₂O₂ was more pronounced than that of other free radicals (O₂ − and OH − ), with albumin and prolamin exhibiting heightened antioxidant activities. Notably, the PBP in various protein fractions showed a higher antiproliferative capacity than their corresponding PFP, indicating a potential synergistic effect of protein-phenolic interactions that differed between the two cell lines, MKN-28 and HT-29.
Purple rice ( Oryza sativa L.) is a rich in endogenous phenolics and proteins. The naturally occurring interactions between phenolic compounds and proteins have been shown to have beneficial effects on human health. In this study, four protein fractions of purple rice (albumin, prolamin, globulin, and glutelin) were extracted, and both protein-free and protein-bound phenolics (PFP and PBP) were isolated from each protein fraction. The major phenolics compounds identified in different protein fraction included protocatechuic acid, vanillic acid, and ferulic acid. Additionally, the PFP in the albumin fraction exhibited the highest number of anthocyanin glycosides (7 types) among all phenolic compounds, while the remaining compounds were identified only as cornflower-3-glucoside and paeoniflorin-3-glucoside. Moreover, the in vitro antioxidant activity and cancer cell inhibitory effects of PFP and PBP in various protein fraction were investigated using chemiluminescence and cellular assays. The results demonstrated that the inhibitory effect of H₂O₂ was more pronounced than that of other free radicals (O₂ − and OH − ), with albumin and prolamin exhibiting heightened antioxidant activities. Notably, the PBP in various protein fractions showed a higher antiproliferative capacity than their corresponding PFP, indicating a potential synergistic effect of protein-phenolic interactions that differed between the two cell lines, MKN-28 and HT-29.
期刊：
Journal of Colloid and Interface Science,2025年679(Pt B):569-577 ISSN：0021-9797
通讯作者：
Ren, Xiaohui;Ni, Hongwei
作者机构：
[Cao, Wenzhe; Zou, Haoran; Jiang, Xingxin; Zhang, Hua; Zhang, Tian] The State Key Laboratory of Refractories and Metallurgy, Key Laboratory for Ferrous Metallurgy and Resources Utilization of Ministry of Education & Hubei Provincial Key Laboratory for New Processes of Ironmaking and Steel Making, Faculty of Materials, Wuhan University of Science and Technology, Wuhan 430081, China;[Ren, Xiaohui] The State Key Laboratory of Refractories and Metallurgy, Key Laboratory for Ferrous Metallurgy and Resources Utilization of Ministry of Education & Hubei Provincial Key Laboratory for New Processes of Ironmaking and Steel Making, Faculty of Materials, Wuhan University of Science and Technology, Wuhan 430081, China. Electronic address: xhren@wust.edu.cn;[Ma, Feng; Chen, Rongsheng] School of Chemical Engineering and Technology, Wuhan University of Science and Technology, Wuhan, Hubei 430081, China;[Qiao, Hui] Hunan Key Laboratory for Micro-Nano Energy Materials and Devices, and School of Physics and Optoelectronic, Xiangtan University, Hunan 411105, China;[Zhang, Ye] Lab of Optoelectronic Technology for Low Dimensional Nanomaterials, School of Chemistry and Chemical Engineering, University of South China, Hengyang 421001, China
通讯机构：
[Ren, Xiaohui; Ni, Hongwei] T;The State Key Laboratory of Refractories and Metallurgy, Key Laboratory for Ferrous Metallurgy and Resources Utilization of Ministry of Education & Hubei Provincial Key Laboratory for New Processes of Ironmaking and Steel Making, Faculty of Materials, Wuhan University of Science and Technology, Wuhan 430081, China. Electronic address:
摘要：
The exploration of multiphases and 0D/2D heterojunction in transition metal phosphides (TMPs) and transition metal sulfides (TMDs) is of major interest for hydrogen evolution reaction (HER). Herein, a novel combination route where 0D mixed-phased 1T/2H molybdenum sulfide quantum dots (MoS 2 QDs) are uniformly anchored on the 2D CoP x nanosheets is developed. MoS 2 QDs and CoP x were prepared via hydrothermal method and mixed with different ratios (Mo:Co ratios of 2:1, 1:1, and 1:2) and annealed under different temperatures to modulate their application in acidic HER processes. Specifically, 2Mo/1Co exhibited advanced performance for HER in 0.5 M H 2 SO 4 solution and required 14 mV to deliver 10 mA cm −2 and revealed a descended Tafel slope of 75.42 mV dec −1 with 240 h stability except obvious deactivation. The successful design and construction of 0D/2D mixed-dimensional materials would broaden the application of MoS 2 and CoP x for electrocatalytic hydrogen evolution.
The exploration of multiphases and 0D/2D heterojunction in transition metal phosphides (TMPs) and transition metal sulfides (TMDs) is of major interest for hydrogen evolution reaction (HER). Herein, a novel combination route where 0D mixed-phased 1T/2H molybdenum sulfide quantum dots (MoS 2 QDs) are uniformly anchored on the 2D CoP x nanosheets is developed. MoS 2 QDs and CoP x were prepared via hydrothermal method and mixed with different ratios (Mo:Co ratios of 2:1, 1:1, and 1:2) and annealed under different temperatures to modulate their application in acidic HER processes. Specifically, 2Mo/1Co exhibited advanced performance for HER in 0.5 M H 2 SO 4 solution and required 14 mV to deliver 10 mA cm −2 and revealed a descended Tafel slope of 75.42 mV dec −1 with 240 h stability except obvious deactivation. The successful design and construction of 0D/2D mixed-dimensional materials would broaden the application of MoS 2 and CoP x for electrocatalytic hydrogen evolution.
摘要：
Investigations using hot compression tests on a new high-strength weathering steel revealed specific deformation behaviors across different conditions. These tests were performed at temperatures ranging from 850 to 1050°C and at strain rates from 0.01 to 5s(-1). Results indicated that a decrease in the deformation temperature combined with an increase in strain rate notably enhanced both the maximum stress and strain achieved. Notably, above 900°C and with strain rates below 0.1s(-1), the flow stress of the material reached a steady state at certain strain levels. At a strain rate of 1s(-1), irrespective of the temperature, the steel shows a continuous strain hardening behavior, achieving no stable flow stress state. Notably, when the true strain exceeds 0.8, an unusual increase in flow stress occurs, predominantly due to secondary work hardening effects. The microstructural changes in the deformed samples were examined using electron backscatter diffraction (EBSD), which helped elucidate the softening mechanisms inherent in this high-strength steel. Further, processing maps developed from true strains of 0.1-0.9, derived from the experimental flow stress data, suggest controlling the strain within 0.2-0.4 to minimize instability during hot working.
关键词：
substantive CSR motive attribution;symbolic CSR motive attribution;in-role green behavior;extra-role green behavior;green intrinsic motivation
摘要：
Employee green behavior plays an important role in the realization of corporate social responsibility (CSR) goals, and the employee motive attribution of CSR can affect employee green behavior. Therefore, it is important to understand how CSR motive attribution affects their green behavior. However, existing studies rarely establish a direct link between CSR motive attribution and green behavior. Based on the attribution theory, we establish a framework to explore the impact of CSR motive attributions on employees' green behaviors. To examine our theoretical model and research hypotheses, we conducted an experimental study (Study 1) and a multi-wave survey study (Study 2). The combined results show that (substantive and symbolic) CSR motive attributions positively influence in-role green behavior; however, for extra-role green behavior, substantive CSR motive attribution positively influences it, while symbolic CSR motive attribution negatively influences it. Green intrinsic motivation positively moderates the relationship between CSR motive attributions and in-role green behavior and positively moderates the relationship between substantive CSR motive attribution and extra-role green behavior but negatively moderates the relationship between symbolic CSR motive attribution and extra-role green behavior. This research contributes to the literature related to micro-CSR and provides explanations for the favorable and unfavorable environmental results brought on by substantive and symbolic CSR, respectively.
作者机构：
[Fang, Min] Key Laboratory of Edible Oil Quality and Safety, State Administration for Market Regulation, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, College of Food Science and Engineering, Wuhan Polytechnic University, 68Xuefu South Road, Changqing Garden, Wuhan, 430023, China. Electronic address: fangmin0227@126.com;[Gong, Zhiyong; Wang, Zhaojie; Xu, Lin; Fu, Qing; Yang, Qing] Key Laboratory of Edible Oil Quality and Safety, State Administration for Market Regulation, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, College of Food Science and Engineering, Wuhan Polytechnic University, 68Xuefu South Road, Changqing Garden, Wuhan, 430023, China;[Jiang, Xiaoming; Lu, Yuepeng; Yang, Yong] Wuhan Institute for Food and Cosmetic Control, 1137 Jinshan Avenue, Wuhan, 430012, China;[Wu, Yongning] Research Unit of Food Safety, Chinese Academy of Medical Sciences (No. 2019RU014), NHC Key Lab of Food Safety Risk Assessment, China National Center for Food Safety Risk Assessment (CFSA), Beijing 100022, China;[Liu, Xin] Key Laboratory of Edible Oil Quality and Safety, State Administration for Market Regulation, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, College of Food Science and Engineering, Wuhan Polytechnic University, 68Xuefu South Road, Changqing Garden, Wuhan, 430023, China. Electronic address: liuxinhook@whpu.edu.cn
通讯机构：
[Min Fang] K;Key Laboratory of Edible Oil Quality and Safety, State Administration for Market Regulation, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, College of Food Science and Engineering, Wuhan Polytechnic University, 68Xuefu South Road, Changqing Garden, Wuhan, 430023, China
摘要：
The aim of this work was to develop a fluorescence method based on the polydopamine-polyethyleneimine (PDA-PEI) copolymerization, which was subsequently applied for the determination of 3-monochloropropane-1,2-diol (3-MCPD) in food contact papers (FCMs). PEI could provide an alkaline environment and then react with dopamine (DA) to produce copolymers by Michael addition and Schiff-base reactions. This copolymer has a strong fluorescence emission at 527 nm. We found that amino groups of DA and PEI could also react with 3-MCPD in an alkaline medium, which improved the morphology and fluorescence intensity of PDA-PEI copolymers. The fluorescence intensity of the polymers was linear but inversely proportional to the concentration of 3-MCPD in the range of 10.0–500.0 μg kg−1 and the detection limit was 2 μg kg−1. The standard addition method was used in FCMs to demonstrate the practical applicability and the spiked recoveries ranged from 99.8 to 110.3 %. Finally, the levels of 3-MCPD in different FCMs (n = 70) were determined by the proposed method. The detection frequencies ranged from 25 % to 100 % and both the highest detection frequency and levels were observed in kitchen papers. More than half of the samples did not comply with the limits recommended by the German Federal Institute for Risk Assessment, suggesting that 3-MCPD released from FCMs is a major route of human exposure.
The aim of this work was to develop a fluorescence method based on the polydopamine-polyethyleneimine (PDA-PEI) copolymerization, which was subsequently applied for the determination of 3-monochloropropane-1,2-diol (3-MCPD) in food contact papers (FCMs). PEI could provide an alkaline environment and then react with dopamine (DA) to produce copolymers by Michael addition and Schiff-base reactions. This copolymer has a strong fluorescence emission at 527 nm. We found that amino groups of DA and PEI could also react with 3-MCPD in an alkaline medium, which improved the morphology and fluorescence intensity of PDA-PEI copolymers. The fluorescence intensity of the polymers was linear but inversely proportional to the concentration of 3-MCPD in the range of 10.0–500.0 μg kg−1 and the detection limit was 2 μg kg−1. The standard addition method was used in FCMs to demonstrate the practical applicability and the spiked recoveries ranged from 99.8 to 110.3 %. Finally, the levels of 3-MCPD in different FCMs (n = 70) were determined by the proposed method. The detection frequencies ranged from 25 % to 100 % and both the highest detection frequency and levels were observed in kitchen papers. More than half of the samples did not comply with the limits recommended by the German Federal Institute for Risk Assessment, suggesting that 3-MCPD released from FCMs is a major route of human exposure.
摘要：
Based on rigid poly (vinyl benzyl chloride) (PVB) and flexible ethylene vinyl alcohol (EVOH) main chains, anion exchange membranes (AEMs) are prepared using the acetal reaction and the Menshutkin reaction. The crosslinking of rigid and flexible backbones, along with the regulation of hydrophilicity/hydrophobicity by fluorinated side chains on EVOH, endows the resulting AEMs with good mechanical properties. The high ionic conductivity of AEMs originates from the effective ionic aggregation morphology, which is generated from the differences between the two main chains and the fluorinated side chains. Their non-polar main chains with stable acetal groups and β-H-free cations contribute to high alkaline stability of AEMs. Specifically, c IM25-TFBA75-EVOH-PVB possesses an ionic conductivity of 148.5 mS cm −1 at 80 °C, with a swelling degree of 12.3 %. The tensile strength and elongation at break of the sample in a fully hydrated state at 25 °C are 7.0 MPa and 12.2 %, respectively. After immersion in 1 M KOH at 80 °C for 1080 h, it almost retains 90 % of its mass, ion exchange capacity, and conductivity. In fuel cells, it achieves peak power densities of 895 mW cm −2 with H 2 /O 2 and 692 mW cm −2 with H 2 /CO 2 -free air at 80 °C.
Based on rigid poly (vinyl benzyl chloride) (PVB) and flexible ethylene vinyl alcohol (EVOH) main chains, anion exchange membranes (AEMs) are prepared using the acetal reaction and the Menshutkin reaction. The crosslinking of rigid and flexible backbones, along with the regulation of hydrophilicity/hydrophobicity by fluorinated side chains on EVOH, endows the resulting AEMs with good mechanical properties. The high ionic conductivity of AEMs originates from the effective ionic aggregation morphology, which is generated from the differences between the two main chains and the fluorinated side chains. Their non-polar main chains with stable acetal groups and β-H-free cations contribute to high alkaline stability of AEMs. Specifically, c IM25-TFBA75-EVOH-PVB possesses an ionic conductivity of 148.5 mS cm −1 at 80 °C, with a swelling degree of 12.3 %. The tensile strength and elongation at break of the sample in a fully hydrated state at 25 °C are 7.0 MPa and 12.2 %, respectively. After immersion in 1 M KOH at 80 °C for 1080 h, it almost retains 90 % of its mass, ion exchange capacity, and conductivity. In fuel cells, it achieves peak power densities of 895 mW cm −2 with H 2 /O 2 and 692 mW cm −2 with H 2 /CO 2 -free air at 80 °C.
摘要：
The metal-organic framework materials ZIF-8 and UiO-66-NH2, synthesized by hydrothermal methods, were characterized in detail and combined to catalyze the transfer hydrogenation of 5-hydroxymethylfurfural (5-HMF) to 2,5-di(hydroxymethyl)furan (2,5-DHMF) using isopropanol as the hydrogen source. The ZIF-8/UiO-66-NH2 catalyst mixture exhibited abundant Lewis acid-base sites and a hierarchical multiporous structure with a large specific surface area (564.0 m2/g). The conversion of 5-HMF reached 98.2 %, and the selectivity and yield of 2,5-DHMF were 96.2 % and 94.5 %, respectively. The recycling performance of ZIF-8/UiO-66-NH2 was also investigated, and the conversion of 5-HMF and selectivity of 2,5-DHMF significantly decreased to 78.4 % and 79.1 % in the second run, respectively. The loss of −NH2 groups was found to be the main reason for the decrease in activity. However, the catalytic activity of the recovered catalyst can be almost completely restored by the addition of a small amount of fresh UiO-66-NH2 and ZIF-8, and the conversion of 5-HMF and selectivity of 2,5-DHMF were increased to 89.4 % and 95.5 %, respectively. In the proposed reaction mechanism, Lewis acid-base sites play important roles and exhibit a synergistic catalytic effect in the selective conversion of 5-HMF to 2,5-DHMF.
The metal-organic framework materials ZIF-8 and UiO-66-NH2, synthesized by hydrothermal methods, were characterized in detail and combined to catalyze the transfer hydrogenation of 5-hydroxymethylfurfural (5-HMF) to 2,5-di(hydroxymethyl)furan (2,5-DHMF) using isopropanol as the hydrogen source. The ZIF-8/UiO-66-NH2 catalyst mixture exhibited abundant Lewis acid-base sites and a hierarchical multiporous structure with a large specific surface area (564.0 m2/g). The conversion of 5-HMF reached 98.2 %, and the selectivity and yield of 2,5-DHMF were 96.2 % and 94.5 %, respectively. The recycling performance of ZIF-8/UiO-66-NH2 was also investigated, and the conversion of 5-HMF and selectivity of 2,5-DHMF significantly decreased to 78.4 % and 79.1 % in the second run, respectively. The loss of −NH2 groups was found to be the main reason for the decrease in activity. However, the catalytic activity of the recovered catalyst can be almost completely restored by the addition of a small amount of fresh UiO-66-NH2 and ZIF-8, and the conversion of 5-HMF and selectivity of 2,5-DHMF were increased to 89.4 % and 95.5 %, respectively. In the proposed reaction mechanism, Lewis acid-base sites play important roles and exhibit a synergistic catalytic effect in the selective conversion of 5-HMF to 2,5-DHMF.
摘要：
In real-world food safety incidents, hazards are often diverse and coexist simultaneously. Mature single-target detection technologies, while effective, are insufficient for comprehensively evaluating the overall quality and safety of food. As a result, multi-target detection, which enables a more comprehensive assessment of multiple hazards in food, has emerged as a prominent research focus. Nanomaterials have gained significant attention in sensing technologies due to their unique advantages, such as high sensitivity and potential for point-of-care detection. Furthermore, nanomaterials hold great promise for multi-target detection technologies because of their versatile properties, including fluorescence and other characteristics that make them suitable for developing a wide range of detection methods. On the basis of systematically summarizing the research achievements of multi-targets sensing in recent five years, this paper categorizes multi-targets sensing nanoprobes construction strategies into five types according to recognition/interaction relationships between the sensing units and targets. In addition, this review also elaborates some practical cases to further explain the design strategy, detection process and detection system of multi-targets sensing nanoprobes, which provides a novel and directional guidance for food detection and food industry management. Finally, according to the shortcomings of current multi-targets sensing nanoprobes, the prospect and challenges of multi-targets sensing nanoprobes are given.
In real-world food safety incidents, hazards are often diverse and coexist simultaneously. Mature single-target detection technologies, while effective, are insufficient for comprehensively evaluating the overall quality and safety of food. As a result, multi-target detection, which enables a more comprehensive assessment of multiple hazards in food, has emerged as a prominent research focus. Nanomaterials have gained significant attention in sensing technologies due to their unique advantages, such as high sensitivity and potential for point-of-care detection. Furthermore, nanomaterials hold great promise for multi-target detection technologies because of their versatile properties, including fluorescence and other characteristics that make them suitable for developing a wide range of detection methods. On the basis of systematically summarizing the research achievements of multi-targets sensing in recent five years, this paper categorizes multi-targets sensing nanoprobes construction strategies into five types according to recognition/interaction relationships between the sensing units and targets. In addition, this review also elaborates some practical cases to further explain the design strategy, detection process and detection system of multi-targets sensing nanoprobes, which provides a novel and directional guidance for food detection and food industry management. Finally, according to the shortcomings of current multi-targets sensing nanoprobes, the prospect and challenges of multi-targets sensing nanoprobes are given.
摘要：
The effects of different phenolic compounds (Epigallocatechin gallate, EGCG; Catechin, CC; Tannic acid, TA) at different phenol hydroxyl concentrations on the gel strength, water holding capacity (WHC), water distribution, and micro-morphology of surimi gels were investigated. Breaking force and deformation of surimi gels treated with EGCG, CC, and TA were increased by 20%, 15%, 12% and 14%, 7%, 4%, compared with untreated surimi gel. Polyphenols increased the WHC of surimi gels and induced the conversion of α-helix to β-sheet in proteins, which was beneficial to the gelation of surimi gel. Surimi gels treated with polyphenols had a complete three-dimensional network structure and relatively uniform and small pores, especially EGCG cross-linked surimi gel. Molecular docking indicated that the interactions between EGCG, CC, TA and myosin heavy chain II A were mainly hydrogen bond and hydrophobic interaction, and their binding energies were −37, −31, and −26 kJ/mol. The binding energy of polyphenols with myosin heavy chain II A correlates with their cross-linking ability with proteins, and the lower the binding energy is, the stronger the cross-linking ability is. This study provides important information to elucidate the mechanism of phenolic compounds regulating the physical properties of surimi gel.
The effects of different phenolic compounds (Epigallocatechin gallate, EGCG; Catechin, CC; Tannic acid, TA) at different phenol hydroxyl concentrations on the gel strength, water holding capacity (WHC), water distribution, and micro-morphology of surimi gels were investigated. Breaking force and deformation of surimi gels treated with EGCG, CC, and TA were increased by 20%, 15%, 12% and 14%, 7%, 4%, compared with untreated surimi gel. Polyphenols increased the WHC of surimi gels and induced the conversion of α-helix to β-sheet in proteins, which was beneficial to the gelation of surimi gel. Surimi gels treated with polyphenols had a complete three-dimensional network structure and relatively uniform and small pores, especially EGCG cross-linked surimi gel. Molecular docking indicated that the interactions between EGCG, CC, TA and myosin heavy chain II A were mainly hydrogen bond and hydrophobic interaction, and their binding energies were −37, −31, and −26 kJ/mol. The binding energy of polyphenols with myosin heavy chain II A correlates with their cross-linking ability with proteins, and the lower the binding energy is, the stronger the cross-linking ability is. This study provides important information to elucidate the mechanism of phenolic compounds regulating the physical properties of surimi gel.
作者机构：
[Li, Wenzhen] Jockey Club School of Public Health and Primary Care of the Chinese University of Hong Kong, Hong Kong SAR, China. wenzhenli@cuhk.edu.hk;[Li, Wenzhen] Shenzhen Research Institute of the Chinese University of Hong Kong, Shenzhen, China. wenzhenli@cuhk.edu.hk;[Zhao, Zhiya] Department of Social Medicine and Health Management, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, Hubei, China;[Chen, Dajie] Department of Health Services and Management, Wuhan Polytechnic University, Wuhan, 430030, Hubei, China;[Kwan, Mei-Po] Department of Geography and Resource Management, The Chinese University of Hong Kong, Hong Kong SAR, China
关键词：
Anxiety;Depression;Health locus of control;Health risk behavior
摘要：
We aimed to assess the association of health locus of control with anxiety and depression, and explore the mediating effects of health risk behaviors. A multi-stage cluster random sampling method was used among Chinese college students. Logistic regression models were used to explore the associations of health locus of control with anxiety and depression. Structural equation models were used to explore the mediation roles of health risk behaviors in the associations of health locus of control with anxiety and depression. A total of 3,951 college students were included in this study. Internality was associated with lower prevalence of depression (OR = 0.94, 95% CI, 0.91-0.97), powerful others externality was also associated with lower prevalence of anxiety and depression (0.92, 0.88-0.96; 0.93, 0.89-0.96), while chance externality was associated with higher risk of anxiety and depression (1.13, 1.08-1.18; 1.24, 1.20-1.28). The mediated proportion of health risk behaviors in associations of internality, powerful others externality, chance externality with anxiety was 7.55%, 2.37% and 2.18%, respectively. The mediated proportion of health risk behaviors in associations of powerful others externality, chance externality with depression was 10.48% and 2.14%, respectively. Health locus of control is associated with anxiety and depression that are mediated by health risk behaviors.
摘要：
This study aimed to investigate the effects of maternal glycerol monolaurate complex (GML) and antibiotic (acetylisovaleryltylosin tartrate, ATLL) supplementation during late gestation and lactation on the reproductive performance of sows and the growth performance of piglets. In total, 64 pregnant sows were randomly divided into control, antibiotic, 0.1% GML, and 0.2% GML groups. The GML shortened their delivery interval and farrowing duration. The ATLL increased the level of malondialdehyde (MDA) in sows and piglets and enhanced glutathione peroxidase (GSH-Px) in piglets, while reducing the tumor necrosis factor-α (TNF-α) level in sows. The GML tended to increase milk protein in the colostrum and decreased the TNF-α of sows at lactation. Meanwhile, 0.2% GML increased the serum total superoxide dismutase (T-SOD) activity and interleukin-6 level in weaned piglets and decreased the TNF-α level in sows and weaned piglets. Furthermore, ATLL decreased the microbial diversity of sows, and GML tended to increase the microbial diversity of sows and piglets. The ATLL group had an increased relative abundance of Bacteroidota in weaned piglets. The GML decreased the relative abundance of Peptostreptococcales-Tissierellales, Proteobacteria, and the harmful bacteria Romboutsia in sows. Compared with the ATLL group, the 0.2% GML reduced the relative abundance of Bacteroidota in weaned piglets. Interestingly, both ATLL and GML supplementation decreased the relative abundance of harmful bacteria Peptostreptococcaceae in sows. Correlation analysis also found positive effects of ATLL and GML in anti-inflammatory and antioxidant aspects. In conclusion, GML enhanced reproductive and growth performance by improving antioxidant and anti-inflammatory status and maintaining intestinal flora balance, making it a promising alternative to ATLL in future applications.
摘要：
Simple Summary: Intestinal health is related to the healthy and efficient breeding of piglets, which needs to be focused on in the post-antibiotic era. Microecological agents play an important role in improving the intestinal health of piglets; however, many of the mechanisms have not been characterized. In the present study, we present an updated report of Lactobacillus reuteri postbiotics on the growth performance, intestinal flora structure and plasma metabolome of weaned piglets. Our outcomes demonstrate that Lactobacillus reuteri postbiotics improve the antioxidant function and reduce the mortality of piglets by regulating the structure of intestinal flora and upregulating the content of coenzyme Q10 in serum. Our findings provide an important theoretical basis for the application of Lactobacillus reuteri postbiotics in piglet production and provide new data for the healthy and efficient breeding of piglets. Probiotics and their postbiotics have the potential to improve the health and growth performance of piglets, which has brought them widespread attention in the post-antibiotic era. In the present study, the effects of dietary supplementation of Lactobacillus reuteri postbiotics on the growth performance, intestinal flora structure and plasma metabolome of weaned piglets were investigated. A total of 816 healthy male piglets with uniform weight were divided into two treatment groups: piglets in the control (CTR) group were fed with a basic diet, and the ones in the LAC group were fed with the basic diet supplemented with 500 mg/kg Lactobacillus reuteri postbiotics. There were six replicates in each group and 68 piglets in each replicate. The animal trial lasted for 30 days. The feces and blood of piglets were collected for investigation, and the growth performance during the trial was counted. Our outcomes show that dietary supplementation with Lactobacillus reuteri postbiotics had no effect on the growth performance of piglets; however, it reduced the mortality rate of piglets by 6.37%. The levels of total superoxide dismutase in the serum, propionic acid and butyric acid in the feces were elevated, and the content of malondialdehyde in the serum was decreased with Lactobacillus reuteri postbiotics-treated piglets (p < 0.05). The fecal flora sequencing results show that the relative abundance of Firmicutes and monoglobus was upregulated, and the relative abundance of Bacteroides was downregulated with Lactobacillus reuteri postbiotics-treated piglets (p < 0.05). In addition, the levels of propionic acid and butyric acid in the feces were positively correlated with the relative abundance of Firmicutes and negatively correlated with the relative abundance of Bacteroides (p < 0.05). The plasma metabolome results show that dietary supplementation with Lactobacillus reuteri postbiotics raised the level of coenzyme Q10 in the serum, and the abundance of coenzyme Q10 was positively correlated with the relative abundance of Firmicutes and the level of total superoxide dismutase in the serum. In conclusion, dietary supplementation with Lactobacillus reuteri postbiotics contributed to improving the antioxidant function and reducing the mortality of piglets by regulating the structure of intestinal flora and upregulating the content of coenzyme Q10 in serum.
通讯机构：
[Liu, P ; Liu, Y ; Liu, P] T;TianGong Univ, Sch Mat Sci & Engn, State Key Lab Separat Membranes & Membrane Proc, Tianjin 300387, Peoples R China.;Tiangong Univ, Sch Chem, Tianjin 300387, Peoples R China.;Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430023, Peoples R China.;Tiangong Univ, Cangzhou Inst, Cangzhou 061000, Peoples R China.
摘要：
Correction for ‘Potentiating light-harvesting tactics through an A–D–A structure: repolarization of tumor-associated macrophages through phototherapy’ by Pai Liu et al. , J. Mater. Chem. B , 2024, 12 , 7870–7878, https://doi.org/10.1039/D4TB00814F.
摘要：
Arabica coffee, as one of the world's three native coffee species, requires rational planning for its growing areas to ensure ecological and sustainable agricultural development. This study aims to establish a decision-making framework using Geographic Information Systems (GIS) and Multi-Criteria Decision-Making (MCDM), with a focus on assessing the habitat suitability of Arabica coffee in Yunnan Province, China. The impacts of climate, topography, soil, and socio-economic factors were considered by selecting 13 criteria through correlation analysis. Indicator weights were determined using the Best-Worst Method (BWM), while weighted processing was conducted using the Base-Criterion Method (BCM). Sensitivity analysis was performed to verify the accuracy and stability of the model. Additionally, several decision models were evaluated to investigate regionalizing Arabica coffee habitats in Yunnan. The results highlighted that minimum temperature during the coldest month is crucial for evaluation purposes. The BWM-GIS model identified suitable areas comprising 13.55% of the total area as most suitable, 27.46% as suitable, and 59.00% as unsuitable, whereas corresponding values for the BCM-GIS model were 9.97%, 30.43%, and 59.59%. Despite employing different decision-making methods, both models yielded similar and consistent results. The suitable areas mainly encompass Dehong, Pu'er, Lincang, Xishuangbanna, Baoshan, southern Chuxiong, eastern Honghe, southern Yuxi, and parts of Wenshan. BWM-GIS achieved an area under curve (AUC) value of 0.891, while BCM-GIS obtained an AUC value of 0.890, indicating the stability and reliability of the models. Among them, the evaluation process of BCM-GIS was simpler and more realistic. Therefore, it has high feasibility and practical value in practical application. The findings from this study provide a significant scientific foundation for optimizing Yunnan Province.
期刊：
Surface and Coatings Technology,2025年503:132030 ISSN：0257-8972
通讯作者：
Changzhe He<&wdkj&>Mao Zhang
作者机构：
[Ying Wang; Changzhe He; Shuobin Chen; Jian Yu; Cheng Nie; Zhigang Hu; Bin Li; Junsheng Yang] School of Mechanical Engineering, Wuhan Polytechnic University, Wuhan 430023, China;[Yuhua Peng] Artificial Intelligence Institute, Wuchang Institute of Technology, Wuhan 430223, China;[Mao Zhang; Pan Gong] State Key Laboratory of Materials Processing and Die & Mould Technology, School of Materials Science and Engineering, Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan 430074, China
通讯机构：
[Changzhe He; Mao Zhang] S;School of Mechanical Engineering, Wuhan Polytechnic University, Wuhan 430023, China<&wdkj&>State Key Laboratory of Materials Processing and Die & Mould Technology, School of Materials Science and Engineering, Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan 430074, China
摘要：
This study comprehensively investigates the corrosion mechanism of laser-cladded FeCoNiCrCu high-entropy alloy (HEA) coatings fabricated with varying initial powder particle sizes in a 3.5 % NaCl solution. Subsequent to orthogonal experimental optimization, optimal laser cladding parameters were obtained to generate fine coatings with exceptional quality. It's noteworthy that finer initial powder particles possess higher specific surface energy, promoting the formation of coatings with fewer defects. Aside from that, steady increases in corrosion current density and decreases in corrosion potential were observed with coarser initial powder particles, accompanied by lower charge transfer resistance. Corrosion preferentially initiates at surface defects, with more severe defects directly degrading corrosion resistance. The Cu accumulation at these defects generates a less extensively protective passivation film, which hinders the formation of Cr oxides. As evidently demonstrated by XPS analysis, smaller powder particles form protective films with more Cr oxides and fewer Cu oxides in comparison with those formed by larger particles. To sum up, coatings prepared from finer initial powder particle sizes display superior corrosion resistance.
This study comprehensively investigates the corrosion mechanism of laser-cladded FeCoNiCrCu high-entropy alloy (HEA) coatings fabricated with varying initial powder particle sizes in a 3.5 % NaCl solution. Subsequent to orthogonal experimental optimization, optimal laser cladding parameters were obtained to generate fine coatings with exceptional quality. It's noteworthy that finer initial powder particles possess higher specific surface energy, promoting the formation of coatings with fewer defects. Aside from that, steady increases in corrosion current density and decreases in corrosion potential were observed with coarser initial powder particles, accompanied by lower charge transfer resistance. Corrosion preferentially initiates at surface defects, with more severe defects directly degrading corrosion resistance. The Cu accumulation at these defects generates a less extensively protective passivation film, which hinders the formation of Cr oxides. As evidently demonstrated by XPS analysis, smaller powder particles form protective films with more Cr oxides and fewer Cu oxides in comparison with those formed by larger particles. To sum up, coatings prepared from finer initial powder particle sizes display superior corrosion resistance.
关键词：
Gel properties;Gelatinizing properties;Microstructure;Wheat starch;gWPI
摘要：
Glycated whey protein isolate (gWPI) was produced by dry thermal reaction between whey protein isolate (WPI) and lactose, and its influence on the gelatinization, gel properties, and microstructure changes of wheat starch (WS) was systematically studied. Results showed that gWPI significantly inhibited starch gelatinization and improved starch gel properties. RVA and DSC analysis showed that gWPI decreased viscosity and gelatinization enthalpy (ΔH) in a concentration-dependent manner. When gWPI concentration was 12 %, the peak viscosity and ΔH decreased by 36 cP and 3.11 J/g, respectively. gWPI competed with WS for water and inhibited WS water absorption and expansion. Rheological results showed that the viscoelasticity of the gel decreased by adding gWPI and that of WS-gWPI was a pseudoplastic fluid with shear-thinning behavior. In addition, gWPI covered the WS surface in the form of hydrogen bonds, which inhibited the leaching of amylose, thereby reducing the particle size of the gelatinized starch and its iodine binding ability. AFM results showed that gWPI combined with WS weakened the aggregation of starch molecular chains and decreased the height of starch. In conclusion, gWPI can be used as a gelatinizing regulator to improve starch properties.
Glycated whey protein isolate (gWPI) was produced by dry thermal reaction between whey protein isolate (WPI) and lactose, and its influence on the gelatinization, gel properties, and microstructure changes of wheat starch (WS) was systematically studied. Results showed that gWPI significantly inhibited starch gelatinization and improved starch gel properties. RVA and DSC analysis showed that gWPI decreased viscosity and gelatinization enthalpy (ΔH) in a concentration-dependent manner. When gWPI concentration was 12 %, the peak viscosity and ΔH decreased by 36 cP and 3.11 J/g, respectively. gWPI competed with WS for water and inhibited WS water absorption and expansion. Rheological results showed that the viscoelasticity of the gel decreased by adding gWPI and that of WS-gWPI was a pseudoplastic fluid with shear-thinning behavior. In addition, gWPI covered the WS surface in the form of hydrogen bonds, which inhibited the leaching of amylose, thereby reducing the particle size of the gelatinized starch and its iodine binding ability. AFM results showed that gWPI combined with WS weakened the aggregation of starch molecular chains and decreased the height of starch. In conclusion, gWPI can be used as a gelatinizing regulator to improve starch properties.
摘要：
Lignin is the only natural polymer compound containing a benzene ring on earth, and its conversion to monophenolic compounds is attracting more attention. Cu-dopped CuCo 2 O 4 is synthesized and further used to catalyze the oxidative conversion of lignin to monophenolic compounds. It is found that the conversion of lignin is affected by the molar ratio of Cu to Co, the amounts of catalyst and H 2 O 2 , reaction temperature and time, and CuCo 2 O 4 exhibits excellent catalytic performance. Under the optimized reaction conditions, the total yield of monophenolic compounds reaches 21.7%. CuCo 2 O 4 also possesses good recyclable performance, and the total yield of monophenolic compounds slightly drops to 17.6% after four cycles. A plausible mechanism for the conversion of lignin to monophenolic compounds is proposed. During the depolymerization of lignin, C O and C C bonds are broken to form monophenols. This work provides an effective catalyst for the conversion of lignin to monophenol and expands the way of high-value utilization of biomass.
