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Precise amplification-free detection of highly structured RNA with an enhanced SCas12a assay

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成果类型:
期刊论文
作者:
Zhang, Junqi;Yin, Wenhao;Jiang, Qingyuan;Mao, Wuxiang;Deng, Wenwen;...
通讯作者:
Wuxiang Mao<&wdkj&>Jie Qiao<&wdkj&>Yi Liu
作者机构:
[Zhang, Junqi; Yin, Wenhao; Jin, Shuqi; Deng, Wenwen; Wang, Xinping; Jiang, Qingyuan] State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, Hubei, 430042, China
[Yin, Wenhao] BravoVax Co., Ltd, Wuhan, Hubei, 430075, China
[Mao, Wuxiang] State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, Hubei, 430042, China. wxmao@hubu.edu.cn
[He, Ruyi] Pilot Base of Food Microbial Resources Utilization of Hubei Province, School of Life Science and Technology, Wuhan Polytechnic University, Wuhan, Hubei, 430023, China
[Qiao, Jie] Pilot Base of Food Microbial Resources Utilization of Hubei Province, School of Life Science and Technology, Wuhan Polytechnic University, Wuhan, Hubei, 430023, China. jieqiao@whpu.edu.cn
通讯机构:
[Wuxiang Mao; Yi Liu] S
[Jie Qiao] P
State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, China<&wdkj&>State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, China<&wdkj&>BravoVax Co., Ltd, Wuhan, China<&wdkj&>Pilot Base of Food Microbial Resources Utilization of Hubei Province, School of Life Science and Technology, Wuhan Polytechnic University, Wuhan, China<&wdkj&>State Key Laboratory of Esophageal Cancer Prevention & Treatment, Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou, China
语种:
英文
期刊:
Communications Biology
ISSN:
2399-3642
年:
2025
卷:
8
期:
1
页码:
366
机构署名:
本校为第一且通讯机构
摘要:
The CRISPR/Cas12a system has revolutionized molecular diagnostics, yet the direct detection of RNA, particularly those with complex structures, remains a significant challenge. Here, we present an updated SCas12a system, termed SCas12aV2, which enables precise, amplification-free detection of highly structured RNA molecules. By optimizing the length of scaffold RNA, targeting asymmetric structures, and utilizing dsDNA-ssDNA hybrid activators, we have significantly reduced steric hindrance in the detection system, thereby markedly enhancing both sensitivity and kinetics compared to traditional ...

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