Precise amplification-free detection of highly structured RNA with an enhanced SCas12a assay

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成果类型：

期刊论文

作者：

Zhang, Junqi;Yin, Wenhao;Jiang, Qingyuan;Mao, Wuxiang*;Deng, Wenwen;...

通讯作者：

Mao, Wuxiang;Liu, Y;Qiao, J

作者机构：

[Mao, Wuxiang; Jin, Shuqi; Zhang, Junqi; Yin, Wenhao; Liu, Yi; Deng, Wenwen; Wang, Xinping; Jiang, Qingyuan] Hubei Univ, Sch Life Sci, State Key Lab Biocatalysis & Enzyme Engn, Wuhan 430042, Hubei, Peoples R China.

[Yin, Wenhao; Liu, Yi] BravoVax Co Ltd, Wuhan 430075, Hubei, Peoples R China.

[Qiao, Jie; He, Ruyi; Qiao, J] Wuhan Polytech Univ, Sch Life Sci & Technol, Pilot Base Food Microbial Resources Utilizat Hubei, Wuhan 430023, Hubei, Peoples R China.

[Qiao, Jie; Qiao, J] Zhengzhou Univ, Affiliated Hosp 1, Dept Oral & Maxillofacial Surg, State Key Lab Esophageal Canc Prevent & Treatment, Zhengzhou 450001, Peoples R China.

通讯机构：

[Qiao, J ] W

[Mao, WX; Liu, Y ] H

Hubei Univ, Sch Life Sci, State Key Lab Biocatalysis & Enzyme Engn, Wuhan 430042, Hubei, Peoples R China.

BravoVax Co Ltd, Wuhan 430075, Hubei, Peoples R China.

Wuhan Polytech Univ, Sch Life Sci & Technol, Pilot Base Food Microbial Resources Utilizat Hubei, Wuhan 430023, Hubei, Peoples R China.

语种：

英文

期刊：

Communications Biology

ISSN：

2399-3642

年：

2025

卷：

期：

页码：

366

DOI：

10.1038/s42003-025-07806-5

基金类别：

National Key Research and Development Program of China [2022YFC2304304]; Science and Technology Innovation Talent Plan of Hubei Province [2023DJC136]; Open Funding Project of the State Key Laboratory of Esophageal Cancer Prevention [K2022-008]; Open Funding Project of the State Key Laboratory of Biocatalysis and Enzyme Engineering [SKLBEE2022022]; Wuhan Polytechnic University [2022RZ031]

机构署名：

本校为通讯机构

摘要：

The CRISPR/Cas12a system has revolutionized molecular diagnostics, yet the direct detection of RNA, particularly those with complex structures, remains a significant challenge. Here, we present an updated SCas12a system, termed SCas12aV2, which enables precise, amplification-free detection of highly structured RNA molecules. By optimizing the length of scaffold RNA, targeting asymmetric structures, and utilizing dsDNA-ssDNA hybrid activators, we have significantly reduced steric hindrance in the detection system, thereby markedly enhancing both sensitivity and kinetics compared to traditional ...

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Precise amplification-free detection of highly structured RNA with an enhanced SCas12a assay

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