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Recombinant Escherichia coli Strains with Inducible Campylobacter jejuni Single Domain Hemoglobin CHb Expression Exhibited Improved Cell Growth in Bioreactor Culture

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成果类型:
期刊论文
作者:
Xu, Li;Xiong, Wei;Yang, Jiang-Ke*;Li, Jia;Tao, Xing-Wu
通讯作者:
Yang, Jiang-Ke
作者机构:
[Xu, Li; Li, Jia; Tao, Xing-Wu; Yang, Jiang-Ke; Xiong, Wei] Wuhan Polytech Univ, Coll Biol & Pharmaceut Engn, Wuhan, Peoples R China.
通讯机构:
[Yang, Jiang-Ke] W
Wuhan Polytech Univ, Coll Biol & Pharmaceut Engn, Wuhan, Peoples R China.
语种:
英文
关键词:
Hemoglobin;Oxygen;Cell growth;Dissolved oxygen;Polymerase chain reaction;Reverse transcriptase-polymerase chain reaction;Gene expression;Recombination-based assay
期刊:
PLOS ONE
ISSN:
1932-6203
年:
2015
卷:
10
期:
3
页码:
e0116503
基金类别:
Science and Technology Supporting Program of Hubei Province; Science and Technology Research Program of the Education Department of Hubei Province [D20131703]; Chinese Ministry of EducationMinistry of Education, China [212118]
机构署名:
本校为第一且通讯机构
院系归属:
生命科学与技术学院
摘要:
Maintaining an appropriate concentration of dissolved oxygen in aqueous solution is critical for efficient operation of a bioreactor, requiring sophisticated engineering design and a system of regulation to maximize oxygen transfer from the injected air bubbles to the cells. Bacterial hemoglobins are oxygen-binding proteins that transfer oxygen from the environment to metabolic processes and allow bacteria to grow even under microaerophilic conditions. To improve the oxygen utilization efficiency of cells and overcome the oxygen shortage in bioreactors, the gene coding for the Campylobacter je...

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