Construction and characterization of a chimeric lysin ClyV with improved bactericidal activity against Streptococcus agalactiae in vitro and in vivo

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作者信息关键词期刊信息基础信息归属信息摘要

成果类型：

期刊论文

作者：

Huang, Li;Luo, Dehua;Gondil, Vijay S.;Gong, Yujing;Jia, Minghui;...

通讯作者：

Hu, Shencai;Yang, Hang;Wei, Hongping

作者机构：

[Huang, Li; Yan, Dazhong; Hu, Shencai] Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Wuhan 430023, Peoples R China.

[Luo, Dehua; Gong, Yujing; He, Jin; Jia, Minghui] Huazhong Agr Univ, Coll Life Sci & Technol, State Key Lab Agr Microbiol, Wuhan 430070, Peoples R China.

[Yang, H; Wei, Hongping; Yang, Hang; Gondil, Vijay S.] Chinese Acad Sci, Ctr Emerging Infect Dis, Wuhan Inst Virol, CAS Key Lab Special Pathogens & Biosafety, Wuhan 430071, Peoples R China.

通讯机构：

[Hu, Shencai] W

[Yang, H; Wei, HP] C

Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Wuhan 430023, Peoples R China.

Chinese Acad Sci, Ctr Emerging Infect Dis, Wuhan Inst Virol, CAS Key Lab Special Pathogens & Biosafety, Wuhan 430071, Peoples R China.

语种：

英文

关键词：

Bacteriophage lysin;Chimeric lysin;GBS;Therapeutics;Susceptibility

期刊：

Applied Microbiology and Biotechnology

ISSN：

0175-7598

年：

2020

卷：

104

期：

页码：

1609-1619

DOI：

10.1007/s00253-019-10325-z

基金类别：

This study was funded by the Wuhan Yellow Crane Talents (Science) Program (to HY), the Youth Innovation Promotion Association CAS (to HY), and the National Natural Science Foundation of China (No. 31770192 to HY and No. 31570175 to HW).

机构署名：

本校为第一且通讯机构

院系归属：

生命科学与技术学院

摘要：

The emergence of antibiotic-resistant beta-hemolytic Streptococcus agalactiae strains poses increasing threat to human beings globally. As an attempt to create a novel lysin with improved activity against S. agalactiae, a chimeric lysin, ClyV, was constructed by fusing the enzymatically active domain (EAD) from PlyGBS lysin (GBS180) and the cell wall binding domain (CBD) from PlyV12 lysin (V12CBD). Plate lysis assay combined with lytic kinetic analysis demonstrated that ClyV has improved activity than its parental enzymatic domain GBS180 agains...

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Construction and characterization of a chimeric lysin ClyV with improved bactericidal activity against Streptococcus agalactiae in vitro and in vivo

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