[Xiao, Anhong; Bi, Jie; Liu, Xiaodan; Shu, Zaixi; Dai, Huang; Wang, Jiahua] Wuhan Polytech Univ, Minist Educ, Key Lab Deep Proc Major Grain & Oil, Wuhan 430023, Peoples R China.
通讯机构：
[Jiahua Wang] C
College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, 430023, People's Republic of China<&wdkj&>Key Laboratory for Deep Processing of Major Grain and Oil (Wuhan Polytechnic University), Ministry of Education, Wuhan, 430023, People's Republic of China
Key Laboratory for Deep Processing of Major Grain and Oil (Wuhan Polytechnic University) [2019GYBQGDKFA01, 2020JYBQGDKFB10]
机构署名：
本校为第一机构
院系归属：
食品科学与工程学院
摘要：
There is an urgent need to measure aflatoxin B(1) (AFB(1)) in food to prevent contaminated food consumption. In this work, a novel colorimetric enzyme-linked immunosorbent assay (ELISA) was developed for the detection of AFB(1) using curcumin as a colorimetric indicator. An indirect competitive enzyme-label immunoassay was developed using urease and rabbit anti-mouse immunoglobulin G labeled with gold nanoparticles as the signal-transduction tag. Urease catalyzed the hydrolysis of urea to produce ammonia, which increased the pH of the solution....
