期刊论文

Shu, S.-H.

中文

中草药

0253-2670

2023

54

12

3962-3970

10.7501/j.issn.0253-2670.2023.12.022

81872948:国家自然科学基金 2060302-1907-05:名贵中药资源可持续利用能力建设项目

本校为其他机构

目的 筛选与茯苓Poriacocos生长发育相关的细胞色素P450基因序列，进一步丰富对茯苓生长发育理解。方法通过分子克隆到1个茯苓细胞色素P450基因Pc CYP，并利用在线工具分析其序列，预测蛋白的理化性质、结构域等分子特征；利用MEGA5.0分别对氨基酸进行多序列比对和进化关系分析，并采用q RT-PCR方法检测不同发酵培养时间6、8、10d的相对表达量。结果 从茯苓中克隆到的Pc CYP存在可变性剪切，2个选择性剪切体PcCYP1和Pc CYP2CDS区长度分别为1590、1542bp，分别编码529、513个氨基酸。氨基酸序列分析发现其均具有保守的P450结构域，且具有信号肽和多个磷酸化修饰位点，属于分泌型的不稳定亲水蛋白...

Objective A cytochrome P450 gene sequence related to the growth and development of Poria cocos was screened and reported to further enrich the understanding of the growth and development of P. cocos. Methods In this study, a cytochorome P450 gene PcCYP was cloned from P. cocos, and some bioinformatics tools were used to predict the physical and chemical properties of the protein, as well as the molecular characteristics of the domain; MEGA 5.0 was used to perform multi-sequence alignment and phylogenetic analysis of amino acids. The gene expres...