期刊论文

中文

食品工业

1004-471X

2014

35

7

28-31

“十二五”农村领域国家科技计划课题“油料低温制油关键技术和装备研究与示范”（2011BAD02B02）；

本校为第一机构

利用中性蛋白酶对油茶籽蛋白进行酶解的工艺研究。以pH、酶解温度、酶解时间、加酶量、底物浓度为单因素进行了单因素酶解研究,再在单因素基础上以水解度为响应值对酶解条件进行响应面分析,得到最佳工艺条件为:酶解温度53.5℃、pH 7、加酶量4 364.0 U/g底物、酶解时间4 h,底物浓度2.9%。

Neutral protease is used to enzymatically hydrolyze Camellia oleifera seed protein. Relationships among the degree of hydrolysis （DH）, substrate concentration, enzyme/substrate, duration time, temperature and pH were studied thoroughly. The response surface methodology was adopted to obtain the optimum conditions of the hydrolysis with neutral protease as enzyme/substmte of 4 364.0 U/g, duration time of 4 h, temperature of 53.5 ℃...