The suitable conditions of phospholipase C enzyme activity and the optimization solution of rapeseed oil degumming was explored. The phospholipase C enzymatic activity conditions were detected by using egg yolk agar plate method and NPPC method; On the basis of single factor experiments, reaction temperature, pH, time and enzyme concentration were optimized by the Box-Behnken design and response surface. The optimum reaction temperature and pH of phospholipase C were 57 ℃ and 7.0 respectively; The best optimization scheme for enzymatic degummi...
