期刊论文

中文

食品工业

1004-471X

2016

37

9

184-187

国家粮食局粮食公益性行业科研专项（201313012-03）；

本校为第一机构

为了使裂壶藻（Schizochytrium limacinum）突变株能够高产DHA,需要对培养条件进行优化。首先通过单因素试验考察接种量、培养温度、装液量和培养基初始pH等因素对二十二碳六烯酸产量的影响,再运用L9（34）正交试验确定培养条件为:接种量6%,培养温度26℃,初始pH 6.5,装液量60 mL/500 mL。在此条件下,油脂产量为15.11 g/L,DHA产量为6.18 g/L。优化后的DHA产量比以前提高了10.92%。该优化结果在同类研究中处于相对较高水平,对于工业化生产提供了科学依据。

In order to make Schizochytrium limacinummutant strain produce more DHA, cultivation condition needs to be optimized. First, investigate the influence of inoculation quantity, culture temperature, loading capacity, initial pH of medium and other factors to the production of DHA through single factor test. Then, use L9（3^4） orthogonal experiment to ensure the cultivation condition： inoculation quantity 4%, culture temperature 26 ℃, loading capacity 60 mL/500 mL, and initial pH 6.5. At these conditions, the production of oil is 15.11 g/L, the...