期刊论文

Zhang, SJ;Zhou, A

英文

Virus Research

0168-1702

2022

314

198738

10.1016/j.virusres.2022.198738

Author contributions A.Z generated the pig genome-wide gRNA library; J.H.J designed and conducted the experiments, wrote this paper. Y.W and G.L analyzed the data from the deep sequencing of survival cells; A.S, Y.S, A.S and K.W helped in the write up and editing of manuscript. S.H.Z supplied the PK15-Cas9 cell line. S.Z designed the experiments and acquired the funding. This study was supported from the National Natural Science Foundation of China (31872328, 31572367).

本校为通讯机构

Porcine reproductive and respiratory syndrome (PRRS), a viral infection caused by PRRS virus (PRRSV) can result in severe reproductive failure, and respiratory disease in the pigs thus causing enormous economic losses to the global swine industry. Although the cellular receptors for PRRSV have been identified, but mechanisms underlying PPRSV replication remain obscure. Here, we have performed a genome-scale CRISPR/Cas9 knockout screen in the pig kidney cells with PRRSV. Several genes were found to be highly enriched post-PRRSV selection, just like KxDL Motif Containing 1(KXD1), Proteasome 26S ...