期刊论文

Chen, S.

中文

应用与环境生物学报

1006-687X

2012

18

3

450-454

10.3724/SP.J.1145.2012.00450

国家自然科学基金项目（No.30970036）资助~~；

本校为其他机构

聚γ-谷氨酸( γ-PGA)是一种应用前景良好的生物高分子材料.比较了蔗糖诱导的枯草芽胞杆菌果聚糖蔗糖酶基因(SacB)启动子和地衣芽胞杆菌α-淀粉酶基凶启动子对γ-PGA降解酶基因ywtD在地衣芽胞杆菌中加强表达的影响.分别用SacB基因启动子和α-淀粉酶启动子构建了穿梭表达载体pHY300-SYT和pHY300-PYT,通过电转化地衣芽胞杆菌WX-02获得重组子SYT和PYT.酶活测定结果显示SYT和PYT中γ-PGA降解酶基因ywtD得到加强表达,摇瓶发酵结果显示两个重组菌株的γ-PGA相对分子质量都由1 000 000～1 200 000降低为800 000～900 000,PYT的γ-PGA产量较对照菌株PLK提高了33％,由13.50 g L-1提高到17.97 g L-1,而SYT...

Poly-γ-glutamate (γ-PGA) is a biopolymer material that has a good application prospect. The effect of two promoters on the ywtD gene expression in Bacillus licheniformis WX-02 was investigated in this study to obtain highly expressed y-PGA depolymerase YwtD. Two recombinants of B. licheniformis WX-02, SYT and PYT, in which ywtD gene was expressed from the control of SacB promoter and a-amylase gene promoter, respectively, were constructed. The enzyme activity assay showed that the expression of YwtD proteins were enhanced in the recombinants ...