Effect of rosmarinic acid covalent conjugation on the structure and allergenic capacity of ovalbumin

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成果类型：

期刊论文

作者：

Shang, Jieli;Zeng, Hao-Long;Jia, Binmei;Wang, Xuanpei;Xu, Lin;...

通讯作者：

Yang, Q

作者机构：

[Wang, Xuanpei; Gong, Zhiyong; Xu, Lin; Liu, Xin; Shang, Jieli; Fang, Min; Yang, Qing; Jia, Binmei] Wuhan Polytech Univ, Hubei Key Lab Proc & Transformat Agr Prod, Wuhan 430023, Peoples R China.

[Wang, Xuanpei; Gong, Zhiyong; Xu, Lin; Liu, Xin; Shang, Jieli; Fang, Min; Yang, Qing; Jia, Binmei] Wuhan Polytech Univ, Key Res Inst Humanities & Social Hubei Prov, Food Safety Res Ctr, Wuhan 430023, Peoples R China.

[Zeng, Hao-Long] Huazhong Univ Sci & Technol, Tongji Hosp, Tongji Med Coll, Dept Lab Med, Wuhan 430030, Peoples R China.

通讯机构：

[Yang, Q ] W

Wuhan Polytech Univ, Hubei Key Lab Proc & Transformat Agr Prod, Wuhan 430023, Peoples R China.

语种：

英文

关键词：

Ovalbumin;Rosmarinic acid;Allergenic capacity

期刊：

LWT

ISSN：

0023-6438

年：

2024

卷：

197

页码：

115869

DOI：

10.1016/j.lwt.2024.115869

基金类别：

Open Research Fund Program of Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education, China [NJZ2022009]

机构署名：

本校为第一且通讯机构

院系归属：

食品科学与工程学院

摘要：

Ovalbumin (OVA), a major allergen in egg -induced food allergy, is widely used in the food industry. This study investigated the structural and antigenic changes of OVA after covalent conjugation with rosmarinic acid (RA). The SDS-PAGE and RA binding equivalent analysis confirmed that both the radical and alkaline methods successfully prepared the OVA -RA conjugates. LC-MS/MS assay identified 20 and 15 modified sites of RA on OVA in the radical and alkaline OVA -RA conjugates, respectively. The structural changes of OVA were indicated by fluorescence spectroscopy, Fourier transform infrared (F...

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Effect of rosmarinic acid covalent conjugation on the structure and allergenic capacity of ovalbumin

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