期刊论文

Yan, Da-Zhong

英文

World Journal of Microbiology and Biotechnology

0959-3993

2015

31

2

371-377

10.1007/s11274-014-1789-7

Natural Science Foundation of Hubei Province of ChinaNatural Science Foundation of Hubei Province [2008CDB067]; National Natural Science Foundation of China (NSFC)National Natural Science Foundation of China (NSFC) [31270112]

本校为第一且通讯机构

A consortium comprised of an engineered Escherichia coli DH5α and a natural pentachlorophenol (PCP) degrader, Sphingobium chlorophenolicum ATCC 39723, was assembled for degradation of hexachlorobenzene (HCB), a persistent organic pollutant. The engineered E. coli strain, harbouring a gene cassette (camA + camB + camC) that encodes the F87W/Y96F/L244A/V247L mutant of cytochrome P-450cam (CYP101), oxidised HCB to PCP. The resulting PCP was then further completely degraded by ATCC 39723. The results showed that almost 40 % of 4 μM HCB was ...