期刊论文

Binding Effects of Chinese Hamster Ovary Cells Transfected with Very LDL Receptor and Its Mutants Deleted Different Repeats with β-Very LDL

中文

中国动脉硬化杂志

1007-3949

2002

10

1

6-9

10.3969/j.issn.1007-3949.2002.01.002

国家自然科学基金 （39670 1 62 ）资助；

本校为第一机构

为了探索极低密度脂蛋白受体配体结合域中8个重复序列在结合脂蛋白中的作用。利用基因缺失诱变的方法，构建了不同重复序列缺失的极低密度脂蛋白受体缺失突变真核表达载体，并将各重组体转移至体外培养的中国仓鼠卵巢细胞。通过逆转录-聚合酶链反应检测到外源基因的表达。转染细胞与DiI标记的β-极低密度脂蛋白结合实验发现：重复序列1，2缺失的极低密度脂蛋白受体结合β-极低密度脂蛋白的能力明显降低。为进一步研究极低密度蛋白受体结构与功能的关系奠定了基础。

Aim To dissect the contribution of each repeat of the ligand binding domain of the VLDL receptor to ligand binding. Methods Several VLDLR recombinants lacking different repeat(s) were constructed by the method of oligonucle-otide-directed mutagenesis and then introduced into CHO cells by transfecdon . The ability of the mutant receptor to bind with (3-VT.DT. was measured. Results The result of the binding experiments showed that repeat 1 and 2 are the most important re-peats required for (3- VLDL binding . Conclusion These result...