National Natural Science Foundation of China [32072281]; Shandong Provincial Natural Science Foundation [ZR2024MC212]; Youth Innovation Program of the Chinese Academy of Agricultural Sciences [Y2024QC12]
机构署名：
本校为其他机构
院系归属：
食品科学与工程学院
摘要：
Starch degradation plays a central role in fruit ripening. The β-amylase (Bam) catalysts starch degradation of apple fruit. However, the molecular mechanism regulating Bam gene expression in apples remains unclear. Using yeast one-hybrid library screening, we identified a transcription factor MdGTL1a that directly binds to the MdBam8 promoter. This bind was verified by using an electrophoretic mobility shift assay and confirmed to enhance the promotor activity of MdBam8 by promoter-β-glucuronidase transactivation assay. Transient over-express...
