期刊论文

Improving the thermotolerance of mannanase through molecular improvement

中文

湖北农业科学

0439-8114

2023

62

12

151-153,162

10.14088/j.cnki.issn0439-8114.2023.12.027

湖北省技术创新重大项目(2018ABA093)。

本校为第一机构

以来源于嗜热放线菌(Thermobifida fusca)的甘露聚糖酶基因(KJ806638)为研究对象,利用易错PCR方法对其进行改造,获得了耐热性进一步提高的甘露聚糖酶。筛选获得的N16I突变株,其最适反应温度从72...展开更多 以来源于嗜热放线菌(Thermobifida fusca)的甘露聚糖酶基因(KJ806638)为研究对象,利用易错PCR方法对其进行改造,获得了耐热性进一步提高的甘露聚糖酶。筛选获得的N16I突变株,其最适反应温度从72.5℃提升到77.5℃。差示扫描荧光定量(DSF)检测结果显示,酶蛋白质的表观解链温度Tm从61.9℃提升到63.2℃。收起

The mannanase gene(KJ806638)from Thermobifida fusca was used as the research object,and it was modified by the er⁃ror-prone PCR method,and the heat resistance of mannanase was further improved.The optimum reaction temperature of N16I mutant was increased from 72.5℃to 77...MORE The mannanase gene(KJ806638)from Thermobifida fusca was used as the research object,and it was modified by the er⁃ror-prone PCR method,and the heat resistance of mannanase was further improved.The optimum reaction temperature of N16I mutant was increased from 72.5℃to ...