期刊论文

Sui, Zhiwei

英文

FOODBORNE PATHOGENS AND DISEASE

1535-3141

2021

18

5

346-353

10.1089/fpd.2020.2894

This study was supported by grant 2017YFF0204602 from the National Key Research and Development Program of China, grant 2019MK114 from the Science and Technology Program of the State Administration for Market Regulation, grant AKY1958 and grant AKY1818 from the National Institute of Metrology, P. R. China, grant 2018KJ34 from Nanjing Customs Project, and grant JC2018094 from Nan-tong Customs Project.

本校为其他机构

A flow cytometry (FCM)-based method was developed for the faster detection of Staphylococcus aureus in milk and milk powder. Viable S. aureus cells were recognized by highly selective, fluorescently labeled antibodies and Propidium Iodide, and then analyzed by FCM. Using a 5-h pre-enrichment period, the method could detect low numbers of S. aureus cells in 6 h, with a limit of detection of 7.50 cells/mL in milk and 8.30 cells/g in milk powder. The established method was compared with the plate-based method using 75 ultra-high-temperature-treate...