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DNA barcoding coupled with high resolution melting for rapid identification of Ardisia gigantifolia and its toxic adulterants

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成果类型:
期刊论文
作者:
Zhao, Bo;Xiong, Chao;Wu, Lan;Xiang, Li;Shi, Yuhua;...
通讯作者:
Sun, W.;Chen, S.
作者机构:
[Sun, Wei; Shi, Yuhua; Zhao, Bo; Wu, Lan; Xiang, Li; Xiong, Chao; Chen, Shilin] China Acad Chinese Med Sci, Inst Chinese Mat Med, Key Lab Beijing Identificat & Safety Evaluat Chin, Beijing, Peoples R China.
[Xiong, Chao] Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Dept Food & Drug Resources & Hlth Food Innovat, Wuhan, Hubei, Peoples R China.
通讯机构:
[Wei Sun; Shilin Chen] K
Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, PR China<&wdkj&>Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, PR China
语种:
英文
关键词:
Ardisia gigantifolia;DNA barcoding;ITS2 and psbA-trnH;Bar-HRM;Rapid identification
期刊:
Biotechnology & Biotechnological Equipment
ISSN:
1310-2818
年:
2021
卷:
35
期:
1
页码:
641-649
基金类别:
This work was supported by the Major Scientific and Technological Special Project for “Significant New Drugs Creation” under Grant (2014ZX09201021), and the project of improving the basic scientific research ability of young and middle-aged teachers in Guangxi Universities in 2020 (2020KY12031).
机构署名:
本校为其他机构
院系归属:
生命科学与技术学院
摘要:
Ardisia gigantifolia is a medicinal plant; its herbal medicinal product has played important roles in the ways of life of people in China. However, commercial products of this herbal medicine are frequently adulterated with Rhododendron molle root and Clerodendrum cyrtophyllum stem. In fact, R. molle has hepatotoxic effects which could pose health issues to the end user. In this study, we applied DNA barcoding coupled with high resolution melting (Bar-HRM) to distinguish A. gigantifolia and adulterant products. Two DNA barcode loci (ITS2 and ps...

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