AbstractConvertible cellulose and lignin were simultaneously isolated from wheat straw using a two-stage process via simply varying temperature and H2SO4 concentration. At the first-stage, cellulose was obtained by pretreating wheat straw at lower temperature and acid concentration using an organosolv process. The purity, yield and recovery rate of cellulose reached 86.8 wt%, 55.2 and 92.8% at 150°C with 1 wt% H2SO4. At the second stage, the residual liquid was further treated at higher temperature and acid concentration, giving 17.4% lignin y...