This paper does research on the condition of zein film formation and its fresh preservation effect. Zein was extracted from corn gluten meal. The best condition of zein film formation was studied by single factor analysis method. The fresh preservation effect of zein film to sliced apples was also observed. The results showed that optimal film formation condition was that the ratio of zein to 80% ethanol was 1:10. The properties of transparency ,waterproofing and antioxidation of zein film...