期刊论文

中文

武汉轻工大学学报

2095-7386

2007

26

1

1-3,10

10.3969/j.issn.1009-4881.2007.01.001

湖北省教育厅重点科研计划项目（2003A007）.

本校为第一机构

通过三亲本杂交和抗药性平板筛选方法，分别获得了带有发光基因和抗药性标记的大豆根瘤菌和巨大芽孢杆菌，并分别接种到黄姜废渣有机肥料中，检测了它们在室温保存条件下的定植数量及盆栽条件下的肥效。结果表明，接入菌剂4周后，黄姜废渣有机肥中的根瘤菌和巨大芽孢杆菌的活菌数分别达到5．9×10^8cfu／g和3．4×10^8cfu／g。大豆施用舍有根瘤菌的生物有机肥后，比施有机肥植株干重增加了24．9％，植株的干重、结瘤数、根瘤鲜重都达到显著性差异。黑麦草施用含巨大芽孢杆菌的生物机肥比施有机肥干重增加了6％，但未达到显著性差异水平。

Sinorhizobium fredii H5 marked by htxAB genes were obtained by tri-parents transconjugating and the Bacillus megaterium B3-KS strain carried Km and Sm resistance was screened. Those strains were inoculated into diosgenin solid fertilize separately, and the numbers of bacteria and the effect of fertilize by pot plant experiments were checked. The results indicated that the numbers of H5 （ LuxAB ） and B3-KS reached about 5.9 ×10^8cfu/g and 3.4× 10^8cfu/g each other in diosgenin solid fertilize after four weeks. The number of nodules, weight o...