The purpose of the paper is to build a module of small molecular receptor fragments eharaetering normal VLDL - R binding function and study the mechanism of VLDL - R binding to ligand. The genes of LBR1- 3 and LBR1-8 were amplified by PCR and the products were inserted into plasmid pET28a （ ＋ ） seperately. The recombinant plasmids were transformed into competent E. coli strain： Rosetta. Recombinant proteins were induced by IPTG. The fusion proteins were purified of Ni^＋ - NTA chromatography. These results lay the foundation of the receptor...
