Tetracycline immunoantigens（ TC-BSA ）and Chlortetracycline immunoantigen （ CTC-BSA ）were prepared via glutaric dialdehyde method and succinic acid method , then the different immunoantigens were injected into New Zealand rabbits in order to prepare different polyclonal antibodies. The special antibodies towards TC and CTC were identified by indirect enzyme-linked immunosorbent assay（ ELISA ）.It was proved that the serum titers of the two kinds of antibodies all reached 1：320000, which provided good immunoantigen for next preparation of t...
