The article discussed the applied study on the extraction of lentinan with Ultrasonic assistive technologies and comfirmed the best techniques for the extraction of lentinan from the rest of edodes. Orthogonal test was adopted to review the influence on the lentinan content of extracting solution worked by the factors that include Ultrasonicwave time, Ultrasonicwave frequency, Ultrasonicwave temperature, the ratio of material and water. And then compared with the traditional extraction way, it showed that the best technic conditions were as fol...