期刊论文

中文

武汉轻工大学学报

2095-7386

2011

30

4

8-11+16

10.3969/j.issn.1009-4881.2011.04.003

本校为第一机构

以鲜切莲藕为研究对象,试验探讨多酚氧化酶( PPO)活性测定的最佳条件、抑制剂对多酚氧化酶的影响,并研究鲜切莲藕在贮藏期间相关指标的变化规律.结果表明:邻苯二酚为底物时,最适波长为410 nm、pH值为7.0、温度为35℃、底物浓度0.09 mol/L及抑制剂组合为Vc浓度0.20％、亚硫酸钠浓度0.10％、柠檬酸浓度0.20％、EDTA浓度0.25％.鲜切莲藕在贮藏期间,褐变度呈上升趋势,水分含量、可溶性蛋白质含量、PPO活性呈先上升后下降的趋势.

The test discusses the optimal experimental conditions determining polyphenol oxidase（PPO） activity,effects of inhibitors on polyphenol oxidase and study on the related index changes of fresh-cut lotus root during storage using the fresh-cut lotus root as the research object.The results showed that when using catechol as substratethe,the most suitable wave length is 410 nm,pH value is 7.0,the temperature is 35 ℃,concentration of substrate 0.09 mol/L and the factor browning inhibition comboination is that the concentration of the Vc is 0.20%,...