期刊论文

中文

武汉轻工大学学报

2095-7386

2013

2

6-10

10.3969/j.issn.1009-4881.2013.02.002

湖北省教育厅2010年高校产学研合作重大项目（C2010040）.

本校为第一机构

利用聚砜卷式超滤、纳滤膜,对鲟鱼鱼皮胶原蛋白肽酶解液精制、脱盐处理,优化其超滤、纳滤条件,以达到脱盐和精制的效果。结果表明,利用3 000 Da超滤膜对鲟鱼胶原蛋白肽精制最优工艺条件为:底物浓度为20 g/L,pH为7,压力为0.2 MPa,温度为20℃;纳滤膜对鲟鱼胶原蛋白肽脱盐最优工艺条件为:底物浓度25 g/L,pH为7,压力为0.5 MPa,温度为20℃。在此条件下,超滤蛋白得率达到80%,纳滤脱盐率达到95.9%以上,短肽回收率高达96.2%,冻干后,蛋白质含量为91%,通过HPLC检测,分子量1 000 Da以下的含量高达97.89%。

The whole research study was aimed at purifying and desalting for sturgeon fish skin collagen peptiae enzymolysis loquid, by means of uhrafiltration and nanofiltration in the different conditions. The results showed that the optimum ultrafihration conditions was substrate concentration is 20 g/L, pH = 7, pressure 0. 2 MPa, temperature is 20℃; and the optimal nanofiltration desalination technology parameters were gained at material liquid concentration 25 g/L, pH = 7, pressure 0.5 MPa, temperature is 20~C. In this conditions, protein yield reac...