期刊论文

Screening and identification of high yield protease producing Bacillus strains

中文

武汉轻工大学学报

Journal of Wuhan Polytechnic University

1009-4881

2014

33

1

6-10

10.3969/j.issn.2095-7386.2014.01.002

本校为第一机构

通过透明圈法从土壤中筛选出20株能分泌胞外蛋白酶的芽孢杆菌,并使用福林-酚试剂法测定了其中透明圈(H/C)值较大的6株和三种市购枯草芽孢杆菌发酵菌液的中性蛋白酶活性.选择中性蛋白酶活性最强的CDY-5菌株(23.44U/mL)及透明圈(H/C)值最大的CDY-1菌株(3.75),进行16S rRNA基因测序后,通过MEGA软件构建系统发育树得出菌株CDY-1为巨大芽孢杆菌,CDY-5为枯草芽孢杆菌.

Abstract ：Twenty extracellular proteases producing Bacillus strains were screened and identified from soil using transparent circle method. Six of them showed larger transparent circle diameter and other three commercially avail- able Bacillus strains were further inoculated in fermentation culture, and the protease activity in the supematant were detected by forint phenol reagent. Sequencing the 16 S rRNA of the strains showed the highest neutral protease activity CDY-5 （23.44 U/mL） and the largest transparent circle diameter CDY-1 （3.75）...