期刊论文

中文

武汉轻工大学学报

2095-7386

2015

34

1

21-25,36

10.3969/j.issn.2095-7386.2015.01.006

本校为第一机构

建立一个高效的分离和分化小鼠成肌细胞的方案,以便探讨MAML1在转基因小鼠中肌管的形成和肌肉发生中的作用。使用细胞分化方法鉴定MAML1在肌肉发生中的作用。使用Western blot检测肌肉发生的标志蛋白肌球蛋白Myosin的表达。研究发现,传代次数少于5次及卫星细胞对成肌细胞在体外形成正确的肌管非常重要。不传代直接进行诱导分化,能促使成肌细胞形成更多的肌管。在MAML1转基因小鼠中,MAML1通过促进形成更多、更粗壮而长的肌管,提高肌球蛋白的蛋白水平来促进肌肉发生。这些MAML1转基因的肌管能够持续存在超过5天,然后才会降解。

Myoblast is a type of embryonic progenitor cell that gives rise to myocytes. MAML1（ mastermind- like1） is a co- activator of NOTCH1 in the process of activation of NOTCH1 target genes. The purpose of this study is to develop an efficient protocol to isolate and differentiate murine myoblasts and to explore the roles of MAML1 in myotube formation or myogenesis in transgenic animals. PCRs were performed to identify MAML1- transgenic mice,immunofluoresencence was performed to identify the expression of myoblast representative protein Myo D,and c...