期刊论文

Effects of TNF-α on murine skeletal muscle myoblasts

中文

武汉轻工大学学报

2095-7386

2015

3

31-36

10.3969/j.issn.2095-7386.2015.03.007

本校为第一机构

肿瘤坏死因子（TNF-α）是一种炎症相关的细胞因子。通过基因组PCR对Maml1基因敲除和MAML1转基因小鼠进行了鉴定,通过去除血清和增加TNF-α来检测小鼠骨骼肌成肌细胞的抗饥饿能力。通过分化培养基诱导、TNF或staurosporine处理来研究成肌细胞肌管形成能力。结果表明,TNF-α促进了成肌细胞抗饥饿的能力,促进成肌细胞分化形成更多、更长、更持久的肌管。同样,MAML1基因也能够促进肌管形成。对进一步了解TNF-α对骨骼肌成肌细胞生理功能的影响提供了借鉴。

Tumor necrosis factor , TNF, is a kind of cytokine related to inflammation .The genome PCRs were per-formed to identify the genotypes of Maml 1-knock-out and MAML1-transgenic mice .The cellular anti-starvation abil-ity was detected by removing serum and adding TNF-α, and myotube formation ability was detected by exchanging with differentiation medium and adding TNF or staurosporine .The results indicate that TNF-αhelps murine skeletal muscle myoblasts the ability of anti-starvation and promotes their differentiation to form more myotubes and ...