期刊论文

中文

武汉轻工大学学报

2095-7386

2016

35

4

37-42

10.3969/j.issn.2095-7386.2016.04.007

武汉市科技人才培育计划创新团队项目（2015070504020222）；

本校为第一机构

黄酮类物质具有优良的防腐抗菌效果,但从植物中提取和纯化技术尚不成熟,为此探讨了油竹干黄酮提取液的大孔树脂纯化工艺。通过7种不同型号大孔树脂对黄酮粗提液的吸附与解吸实验,优选出AB-8型大孔树脂;并得出油竹干黄酮提取液的最佳纯化条件为:1.0 mg/m L黄酮水溶液,上样液p H为6.0,上样速度为1.0 m L/min,最大上样量为170 m L;采用浓度为60%的乙醇进行洗脱,洗脱速度为1.5 m L/min,其用量为120 m L。此条件下纯化效果最佳,竹干黄酮样品纯度由21.3%提高到50.85%。

Flavonoids have excellent antiseptic and antibacterial effect,but the relevant abstraction and purification technology is not mature yet,so this study investigated the purification process of total flavonoids from the extracting solution of Bambusa surrecta by macroporous resin. AB-8 macroporous resin was selected to isolate and purify crude flavonoids extract from seven macroporous resin via adsorption and desorption experiments,and the optimal parameters are as follows： 1. 0 mg / m L crude flavonoids solution with p H value of 6. 0,the flow ...