Using biodiesel as raw material,the unsaturated fatty acid methyl esters were enriched by the method of secondary cooling crystallization, and results were determined by gas chromatograph. Compared to the raw materi-al ,the enriched liquid oil after the secondary cooling crystallization has higher content of unsaturated fatty acid methyl esters and lower freezing point. The content of unsaturated fatty acid methyl esters increased from 61.22% to 87.91% , and the freezing point decreased from 8. 5 ℃ to -4.1 ℃. The enriched liquid oil can not o...