期刊论文

中文

武汉轻工大学学报

2095-7386

2019

38

1

17-21

10.3969/j.issn.2095-7386.2019.01.003

31572572:国家自然科学基金 2017y41:武汉轻工大学校立科研项目

本校为第一机构

为了探讨黄芩苷对副猪嗜血杆菌（HPS）诱导后猪血管内皮细胞黏附分子E-选择素（E-selectin）、细胞间黏附分子1（ICAM-1）和血管细胞黏附分子（VCAM-1）的影响,体外培养猪血管内皮细胞,接种副猪嗜血杆菌。用ELISA法检测细胞培养液中E-selectin、ICAM-1和VCAM-1释放量;用Q-RT PCR法检测血管内皮细胞内E-selectin、ICAM-1和VCAM-1的mRNA表达水平。结果表明:HPS感染猪血管内皮细胞后,细胞培养液中E-selectin、ICAM-1、VCAM-1的释放量和细胞中E-selectin、ICAM-1、VCAM-1的mRNA表达水平都极显著地上调（P

In order to investigate the effect of baicalin on the expression of vascular endothelial cell adhesion molecules(E-selectin,ICAM-1,VCAM-1)induced by Haemophilus parasuis(HPS),porcine vascular endothelial cells were co-cultured with Haemophilus parasuis.The amount of E-selectin,ICAM-1 and VCAM-1 in cell culture medium was detected by ELISA.The mRNA expression levels of E-selectin,ICAM-1 and VCAM-1 in vascular endothelial cells were detected by Q-RT PCR.The results showed that after HPS infection with porcine vascular endothelial cells,the releas...